The abundance,distribution,and enrichment mechanism of harmful trace elements in coals from Guizhou,Southwestern China

Coal seams can enrich a variety of harmful trace elements under specific geological conditions.The spatial distribution of harmful trace elements in coal is extremely uneven,and the distribution characteristics of each element content are different.The harmful elements released in the process of coal mining and utilization will cause serious harm to the environment and the human body.It is of great resource significance to study the geochemistry of coal that affects the enrichment and distribution characteristics of harmful trace elements.Based on the domestic and foreign literature on coal geochemistry in Guizhou published by previous investigators,this study counted 1097 sample data from 23 major coal-producing counties in Guizhou Province,systematically summarized the relevant research results of harmful trace elements in the coal of Guizhou,and revealed the overall distribution and enrichment characteristics of harmful trace elements in the coal of Guizhou.The results show that the average contents of Cd,Pb,Se,Cu,Mo,U,V,As,Hg,and Cr in coal of Guizhou are higher than those in Chinese coal and world coal.A variety of harmful trace elements in the coal of Guizhou have high background values,especially in Liupanshui,Xingyi and Qianbei coalfield.The enrichment of various harmful trace elements in the Late Permian coal in Guizhou is mainly related to the combined action of various geological and geochemical factors.The supply of terrigenous debris and sedimentary environment may be the basic background of the enrichment of harmful elements in western Guizhou,while low-temperature hydrothermal activity and volcanic ash deposition may be the main reasons for the enrichment of harmful elements in southwestern Guizhou.

Major Diseases and Pests of Blueberry in Guizhou Province and Progress in Their Prevention and Control

As the blueberry industry continues to evolve,the effective control of its diseases and pests has become an essential component of local agricultural development.This paper provides a comprehensive overview of the principal types of blueberry diseases and pests in Guizhou Province,along with the corresponding control measures,in order to serve as a valuable reference for blueberry growers.

Tourism Mode of Tea Culture Museum in Guizhou:A Case Study of Meitan Modern Tea Culture Museum Settlement

Since the 14 th Five-Year Plan,tea culture has become very popular.However,due to the lack of brand awareness and overall planning,and the mechanical application of the model outside the province and other tourism products,Guizhou Tea Culture Tourism presents a"strong tea,light culture,weak tourism".In view of this,in order to realize the positioning goal of"building a national demonstration zone for the integration of tea culture and tourism"in the 14 th Five-Year Plan of Guizhou tea industry,and explore the development mode of integration of tea culture and tourism with Guizhou characteristics,this study takes museum tourism as the breakthrough point,Meitan Tea Museum settlement as the research object,and collects Meitan Tea Museum tourism resources.This paper uses SWOT analysis to evaluate the Meitan Tea Culture Museum Tourism model,and provides some recommendations for the development of tea culture museum tourism in Guizhou Province.

Ancient Zhenyuan, Gateway to East Guizhou

This ancient city has a history going back over 2,000 years.It has witnessed the fusion of multiple ethnic cultures including the Miao,Dong,and Han,and hosts a number of cultural heritage sites.THE old town of Zhenyuan is located in Zhenyuan County,Qiandongnan Miao and Dong Autonomous Prefecture in southwest China’s Guizhou Province.Covering an area of just around three square kilometers,it sits on the banks of the Wuyang River and is surrounded by mountains.Zhenyuan has been a county for more than 2,281 years and was the prefectural capital during the Yuan(1271-1368)and Qing(1644-1911)dynasties.It is a multicultural region,with Miao,Dong,and other ethnic minorities making up nearly half of the population.

Chinese New Year Folk Culture Adds Impetus to Guizhou's Colorful Tourism Economy

During the Chinese New Year in 2024,a series of traditional folk activities were held all over Guizhou,attracting a large number of travelers,with the number of received travelers and comprehensive income of Guizhou reaching a new high.

Machine learning-based automatic construction of earthquake catalog for reservoir areas in multiple river basins of Guizhou province,China

Large reservoirs have the risk of reservoir induced seismicity.Accurately detecting and locating microseismic events are crucial when studying reservoir earthquakes.Automatic earthquake monitoring in reservoir areas is one of the effective measures for earthquake disaster prevention and mitigation.In this study,we first applied the automatic location workflow(named LOCFLOW)to process 14-day continuous waveform data from several reservoir areas in different river basins of Guizhou province.Compared with the manual seismic catalog,the recall rate of seismic event detection using the workflow was 83.9%.Of the detected earthquakes,88.9%had an onset time difference below 1 s,81.8%has a deviation in epicenter location within 5 km,and 77.8%had a focal depth difference of less than 5 km,indicating that the workflow has good generalization capacity in reservoir areas.We further applied the workflow to retrospectively process continuous waveform data recorded from 2020 to the first half of 2021 in reservoir areas in multiple river basins of western Guizhou province and identified five times the number of seismic events obtained through manual processing.Compared with manual processing of seismic catalog,the completeness magnitude had decreased from 1.3 to 0.8,and a b-value of 1.25 was calculated for seismicity in western Guizhou province,consistent with the b-values obtained for the reservoir area in previous studies.Our results show that seismicity levels were relatively low around large reservoirs that were impounded over 15 years ago,and there is no significant correlation between the seismicity in these areas and reservoir impoundment.Seismicity patterns were notably different around two large reservoirs that were only impounded about 12 years ago,which may be explained by differences in reservoir storage capacity,the geologic and tectonic settings,hydrogeological characteristics,and active fault the reservoir areas.Prominent seismicity persisted around two large reservoirs that have been impounded for less than 10 years.These events were clustered and had relatively shallow focal depths.The impoundment of the Jiayan Reservoir had not officially begun during this study period,but earthquake location results suggested a high seismicity level in this reservoir area.Therefore,any seismicity in this reservoir area after the official impoundment deserves special attention.

Species Composition and Diversity of Fagus longipetiolata in Leigong Mountain, Guizhou Province

[Objectives]To understand the species composition and diversity of Fagus longipetiolata community.[Methods]The F.longipetiolata community in Guizhou Province was investigated by typical plot method.[Results]There were 107 species of vascular plants belonging to 79 genera and 51 families in Leigong Mountain F.longipetiolata community,including 3 species of pteridophytes belonging to 3 genera and 3 families,104 species of spermatophytes belonging to 76 genera and 48 families,including 3 species of gymnosperms belonging to 3 genera and 3 families,and 101 species of angiosperms belonging to 73 genera and 45 families.The types of Leigong Mountain F.longipetiolata community were divided into 7 formations,and the top 10 important species of each formation were Fagaceae,followed by Lauraceae and Ericaceae.The change trend of tree layer richness index was formation I>formation IV>formation VI>formation III>formation VII>formation II>formation V;the dominance index was formation I>formation IV>formation V>formation VI>formation VII>formation III>formation II;H diversity index was formation V>formation II>formation I>formation III>formation VII>formation VI>formation IV;the evenness index is formation II>formation III>formation VI>formation VII>formation IV>formation I>formation V;the total diversity index of the community,formation I(3.67)was the highest,formation V(2.74)was the lowest,manifested as formation I>formation III>formation VI>formation II>formation IV>formation VII>formation V.[Conclusions]The community stability is closely related to its species composition,and the background species is the basis for the survival of the community;the existence of rare species can further enhance the community diversity index and enhance the community stability.

Problems and Recommendations for Export of Guizhou Tea in the Context of Rural Revitalization Strategy

As a traditional advantageous industry in Guizhou and an important export product,tea plays an important role in the rural revitalization strategy.This paper first introduces the current situation of tea industry and export in Guizhou,then analyzes the problems that arise in the export process,and finally comes up with corresponding countermeasures and recommendations for the export development of tea,hoping to provide certain guidance and reference to the development of tea industry in Guizhou Province.

Coordinated Development of Tourism Economy and Ecological Environment Coupling in Mountainous Provinces:A Case Study of Guizhou

The degree of coupling coordination can reflect the intensity of interaction between multiple-indicator system,and can characterize the harmonious and consistent degree of benign correlation between them.It is an effective measurement method for analyzing the level of coordinated development of tourism economy and ecological environment coupling.Guizhou,a mountainous province,is taken as the research object in this paper.Using entropy method and coupling coordination model,the coordinated development level of tourism economy and ecological environment coupling during 2011-2020 is measured and analyzed,and the coordinated development strategies for the tourism economy and ecological environment coupling in Guizhou,a mountainous province,are proposed.

Temporal and Spatial Variation of Summer Extreme High Temperature in Guizhou Province from 1970 to 2020

Based on the daily maximum temperature data of 31 meteorological observation stations and some statistical methods, the temporal and spatial characteristics of summer extreme high temperature in Guizhou province from 1970 to 2020 are analyzed. The results indicate that: 1) The threshold of extreme high temperature (EHT) in summer in Guizhou province had a large spatial difference, with decreasing characteristics from the northeast to the southwest, it was negatively correlated with the altitude. 2) In most parts of Guizhou province, the extreme high temperature days (EHTD) in summer can reach about 4.2 d, the lowest EHTD occurred in the southernmost part. From June to August, the EHTD gradually increased, especially in Central and eastern parts of Guizhou province. However, the extreme high temperature intensity (EHTI) displayed similar distribution characteristics in summer, June, July and August, with larger value in the northeast part and lower value in the southwest part of Guizhou. 3) EHTD had a rising trend in almost stations, except for the PZ station, the increased range and intensity gradually increased from June to August. But the EHTI had a larger spatial difference, especially in June, it declined in most parts of Guizhou, the declined scope and intensity gradually decreased in July, and completely increased in August, this made EHTI show an increasing trend in summer in most parts the Guizhou province. 4) The averaged EHTD increased by 0.62 d/10a (p 0.1), the significant increase also occurred in August, but it increased insignificantly in June and July. The averaged EHTI had insignificant increase in summer and the three months. In general, the EHTD and EHTI increased in most parts of Guizhou province during the period of 1970-2020, this may be related to the changes of them in August.

Geological characterization and exploration potential of shale gas in the Carboniferous Jiusi Formation,northern Guizhou and Yunnan provinces,SW China

Dark mudstones and shales of the Carboniferous Jiusi Formation are widely developed in northern Guizhou and Yunnan provinces, SW China. However, the distribution, reservoir characterization, and exploration potential of organic-rich shales in this area are yet to be quantified, thus limiting the prospect of shale gas in this area. This study investigates the basic geological conditions of Jiusi shale gas, using core data, well-logs, and some other test data, obtaining the following results. The organic-rich shales are mainly composed of deltaic-to-shallow-shelf deposits, with thickness ranging from 0 to 450 m, and above 350 m around the subsidence center. The organic matter is mainly type Ⅱ kerogen with TOC content of mostly 1%–2%, indicating a moderate maturity. The argillaceous shale reservoirs are indicative of strong heterogeneity, high clay minerals content, low porosity, low permeability, high specific surface area, and relatively developed secondary porosity. The gas-log anomaly intervals obtained from the survey wells have a cumulative thickness that is apparently greater than 200 m, and a few shale intervals showing high desorbed and adsorbed gas contents. Due to complex structures in the study area, conditions responsible for shale gas occurrence and trapping are generally moderate. However, areas having wide and gentle folds with moderate depth of burial reveals relatively favorable conditions of hydrocarbon traps. In contrast with typical marine-continental transitional shales, the Jiusi shale have better geological conditions for shale gas preservation. The analysis of the geological framework and hydrocarbon potential of Carboniferous Jiusi Formation provide more insight for the exploration of Carboniferous shale gas in southern China.

伪狂犬病病毒Guizhou-DY株的分离鉴定及致病性研究

通过采集疑似猪伪狂犬病流产胎儿病料，采用细胞接毒分离培养、病毒蚀斑纯化、病毒的形态结构观察、易感动物接种试验和病毒核酸鉴定分离到1株伪狂犬病病毒（PRV），命名为PRVGuizhou—DY株（GenBank登录号为JX417716）；为了摸清其理化特性，进一步对Guizhou—DY分离株进行了耐热、耐酸及脂溶性敏感性试验、核酸类型鉴定及病毒毒价测定，同时进行病毒致病性试验。分离鉴定结果表明：病料接种Vero细胞盲传至第2代时产生明显CPE，盲传至第4代可出现稳定的CPE；病毒粒子在电镜下呈椭圆形、有囊膜、直径为120～180nm，核内可见呈晶格状排列的病毒包涵体；病毒悬液接种家兔能引起家兔奇痒、麻痹死亡；理化特性研究表明：该病毒是一种耐热不耐酸且对氯仿敏感的DNA病毒，gE全基因序列与PRVGZ—Z1株、Fa株及Ea株的相似性分别为97．60A、98．9％和99．4％，其TCID50为10-9.71·100μL-1，PFu为109·mL-1. 攻毒组猪体温升高，临床症状较为明显，在攻毒后第5天开始出现中和抗体，第21天达到最高。该研究可为PRV病原学研究提供参考。

Influence of Diversity of Prototypical Ethnic Culture in Diversity of Glutinous Rice in Southeast of Guizhou

There were quite abundant prototypical ethnic cultures and plant diversity in Qiandongnan （ Southeast of Guizhou） ,where the primitive natural ecology was also perfectly conserved. It was honored by World Native Culture Fund as One of the Eighteen Ecological and Cultural Conservations in the World. The prototypical ethnic culture of Southeast of Guizhou, as a stable life-style and economic culture type of the local people, came into being in the process of the local minorities＇ communicating, blending and struggling against the natural environment in the long course of history. It could be seen from this research that there were our preliminary research that there are quite abundant resources in Southeast of Guizhou in terms of prototypical ethnic cultures and genetic diversity of glutinous rice. A full reflection of the culture of Glutinous Rice could be seen in the local minority people＇s daily life, production, custom, religious belief, etc. These showed that the formation and great vitality of the genetic diversity of glutinous rice was not only determined by the diversity of local prototypical ethnic cultures but also to its own biotic condition and its adaptation to natural ecology. It was showed that prototypical ethnic cultures had positive impact on the conservation and utilization of Glutinous Rice diversity.

New Macroalgal Fossils from Middle Cambrian Kaili Biota in Guizhou Province, China

Abundant and well-preserved remains of noncalcareous algae and soft-bodied metazoans were collected from Middle Cambrian Kaili biota in Taijiang county, Guizhou Province, China. These remains provide further evidence for the wide geographic distribution of many Burgess Shale taxa. Among the algae, 5 genera (including two new genera) and 5 species are described. They are Marpolia spissa Walcott, Acinocricus stichus Conway Morris and Robison, Udotealga erecta Yang, Eosargassum sawata Yang, and Rhizophyton zhaoyuanlongii Yang. Contrasting the macroalgal fossil assemblage in the Kaili biota with one in the Burgess Shale biota, it is clear that similarity of the Kaili biota and the Burgess Shale biota is reflected by the same content of not only the soft-bodied metazoans, but also the noncalcareous algae.

Genetic Diversity Analysis of Three Guizhou Local Pig Breeds Using Microsatellite DNA Markers

[Objective] The research aimed to provide the genetic basis for the protection, development and utilization of Guizhou local pig breeds. [Method] From 27 pairs of porcine microsatellite primers recommended by Food and Agriculture Organization of the United Nations （FAO） and international Society for Animal Genetics （ISAG）, six pairs （S0155, SW240, IGF1, SW951, SW857, SW24） were selected for microsatellite DNA detection of three Guizhou local pig breeds, including Nuogu Pig, Kele Pig and Guanling Pig. Subsequently, their genetic diversities were analyzed. [ Result] The three pig breeds were high polymorphic at the six microsatellite loci （PIC 〉0.5）. The Nei＇s standard genetic distance of them was 0.206 3 -0.481 5. The genetic distance between Nuogu Pig and Kele Pig Was the closest, and that between Nuogu Pig and Guanling Pig was the furthest. [ Conclusion] The three Guizhou local pig breeds are in high genetic diversities. Nuogu Pig is a special type of Kele Pig, an excellent Chinese local pig breed.

Diagnosis of Mixed Infection of CSFV, PRV and E.coli in a Modern Pig Farm of Guizhou Province

In order to understand the incidence reasons in a scaled pig farm of Guizhou Province, the infected pigs were diagnosed with epidemiological investigation, clinical observation, pathological diagnosis, enzyme-linked immunosorbent assay （ELISA）, PCR/RT-PCR and bacterial isolation and identification. The results showed that the pigs were infected mixedly with classical swine fever virus （CSFV）, pseudorabies virus （PRV） and E. coli. Therefore, some measures should be adopted to control the incidence of CSF, PR and colibacillosis in the pig farm.

Resources of Wild Ornamental Ferns in Guizhou Province and Their Utilization

Through investigating the wild ornamental ferns in Guizhou Province, it is found that there are totally 312 species (including variations), 111 genera and 49 families of such resources in this region, which are classified into terrestrial fern, epiphytic fern, saxicolous fern, vine fern, tree fern and aquatic fern according to their ecological types. The application of ornamental ferns in garden landscaping and development countermeasures are analyzed in this study.

伪狂犬病病毒Guizhou-T1株的分离鉴定及其遗传变异研究

为了解贵州猪伪狂犬病病毒(PRV)流行株的病原形态特征、致病性及遗传变异情况,本实验对收集自贵州规模化猪场伪狂犬病净化过程中PCR检测的阳性病料样品,采用细胞接毒分离培养、病毒的形态结构观察和易感动物接种试验对其病原进行鉴定。结果显示,本研究分离到一株PRV,命名为PRV Guizhou-T1株;并对其进行病毒效价的测定及g E基因核苷酸和氨基酸序列差异性分析。结果显示:PRV Guizhou-T1株gE基因序列与GZ-Z1株和Guizhou-DY株同源性分别为97.5%和99.3%,其TCID_(50)为10^(-10.11)/100μL;PRV Guizhou-T1株在gE基因编码的氨基酸序列第48和第496位均有天冬氨酸(Asp D)的插入,与PRV变异株变异位点一致,且在其第48位缺失酪氨酸(Tyr Y)。表明本研究分离得到了一株PRV变异株。本研究可为贵州PRV病原学的研究提供参考。

Correlation Between the Animal Community Structure and Environmental Factors in Dongbei Cave and Shuijiang Cave of Guizhou Province

Mollusks, arthropods and chordates which were visible to the naked eye were observed and collected in Dongbei and Shuijiang caves of Libo county five times between February and July from 2002 to 2005. Four hundred and forty samples from Dongbei Cave were classified into three phyla, five classes, 10 orders, 20 families and 39 species or groups of species. Four hundred and ninety-eight samples from Shuijiang Cave were classified into three phyla, six classes, 11 orders, 20 families and 25 species or groups of species. Six animal communities were identified in the light belt of the two caves according to their species types and numbers of individuals in the light belt of two cave. The communities which have the highest values of species richness community diversity, maximum diversity, evenness, dominance and community similarity are respectively： B （4. 1059）, H （2.4716）, B （3.3322）, E （0.9042）, C （0.3442） and A - C （0. 5251）. The community diversity and correlation of environmental factors were also studied. The temperature, humidity, content of CO2 and N2, content of organic matter and some inorganic salts in soil were analyzed by Pearson correlation. The results showed that the content of organic matter in soil is positively related to species number, species richness and maximum community diversity, with correlations of 0. 885, 0.909, 0. 868 respectively （two-tailed significance test, P ≤ 0. 05）, and significantly positively related to diversity, with the coefficient of 0.611, （two-tailed significance test, P ≤ 0. 1）. This suggests that the content of organic matter in soil is one of the important elements influencing the community variation of cave animals. Outside the cave, temperature and humidity are important factors impacting on community diver- sity. As the temperature in all seasons in the cave is stable and the humidity is always high （above 90% ）, the temperature and humidity have weak correlation to community diversity in caves.

Temporal and Spatial Distribution Characteristics of Potential Evapotranspiration and Its Sensitivity to Meteorological Factors in Guizhou Province

[Objective] The study aims to discuss the changes of potential evapotran- spiration and its sensitivity to meteorological factors in Guizhou Province, so as to provide important references for assessment of water resources, research of agri- cultural water conservancy and climate change. [Method] Temporal and spatial dis- tribution characteristics of potential evapotranspiration in Guizhou Province from 1961 to 2010 were analyzed, and the sensitivity of potential evapotranspiration to meteo- rological factors in Guizhou Province was studied through correlation analysis. [Re- sult] On the whole, potential evapotranspiration in Guizhou Province was higher in the southwest and the west compared with the northeast and the east. In various seasons, it was the highest in summer, followed by spring and autumn, while it was the lowest in winter. In recent 40 years, annual potential evapotranspiration showed an obvious decreasing trend in most stations of Guizhou Province. The main meteo- rological factors influencing changes of potential evapotranspiration in Guizhou Province were sunshine duration, daily maximum temperature, and daily average relative humidity. [Conclusion] Daily average temperature was not the main meteoro- logical factor affecting changes of potential evapotranspiration in Guizhou Province, while sunshine duration, daily maximum temperature, and daily average relative hu- midity had important effects on potential evapotranspiration in Guizhou Province.