The Paramesotriton caudopunctatus species group is mainly distributed in the karst mountain ecosystems of Guizhou, China. Although some species have been included in previous phylogenetic studies, the evolutionary rel...The Paramesotriton caudopunctatus species group is mainly distributed in the karst mountain ecosystems of Guizhou, China. Although some species have been included in previous phylogenetic studies, the evolutionary relationships and divergence-time of members of this species group as a whole remain unexplored. In this study, we report the sequencing of one protein coding mitochondrial gene fragment(ND2) and one nuclear gene(POMC), and use a combination of phylogenetic analyses and coalescent simulations to explore the cryptic diversity and evolutionary history of the P. caudopunctatus species group. Phylogenetic relationships revealed that the P. caudopunctatus species group is composed of two major groups, i. e., East Clade and Western-South Clade. The divergence-time and ancestral area estimation suggested that the P. caudopunctatus species group likely originated in the Doupeng Mountains in Guizhou, China at 12.34 Ma(95% HPD: 8.30–14.73), and intraspecific divergence began at about 2.17 Ma(95% HPD: 1.39–2.97). This timing coincides with the orogenesis of the Miaoling Mountains during the Late Miocene to early Pleistocene. The delimitation of species in the P. caudopunctatus species group supports the existence of the currently identified species, and consensus was confirmed across methods for the existence of least to two cryptic species within what has been traditionally considered to be P. caudopunctatus species group. This study is of significance for understanding the species formation, dispersal, and diversity of the tailed amphibians in the karst mountains ecosystem of Guizhou and the role of the Miaoling Mountains as a geographical barrier to species dispersal.展开更多
The widely spread Carboniferous-Permian coal seam group in southern China has great potential for coalbed methane resources,but the extensively developed tectonically deformed coal seriously restricts its development....The widely spread Carboniferous-Permian coal seam group in southern China has great potential for coalbed methane resources,but the extensively developed tectonically deformed coal seriously restricts its development.Taking the Dahebian block in western Guizhou as the study area,the geological model of coalbed methane reservoirs in the tectonically deformed coal seam group was established,and the spatial distribution pattern of model parameters was clarified by clustering algorithms and factor analysis.The facies model suggests that the main coal body structures in Nos.1,4,and 7 coal seams are cataclastic coal and granulated coal,whereas the No.11 coal seam is dominated by granulated coal,which has larger thicknesses and spreads more continuously.The in situ permeability of primary undeformed coal,cataclastic coal,granulated coal,and mylonitized coal reservoirs are 0.333 mD,0.931 mD,0.146 mD,and 0.099 mD,respectively,according to the production performance analysis method.The property model constructed by facies-controlled modeling reveals that Nos.1,4,and 7 coal seams have a wider high-permeability area,but the gas content is lower;the high-permeability area in the No.11 coal seam is more limited,but the gas content is higher.The results of the self-organizing map neural network and K-means clustering indicate that the geological model can be divided into 6 clusters,the model parameter characteristics of the 6 clusters are summarized by data analysis in combination with 6 factors extracted by factor analysis,and the application of data analysis results in multi-layer coalbed methane co-development is presented.This study provides ideas for the geological modeling in the tectonically deformed coal seam group and its data analysis.展开更多
调查贵州9个世居少数民族线粒体DNA Region V以及Y染色体DYS287位点多态性情况,为贵州相关少数民族的遗传背景研究提供可参考的分子遗传学数据。采用PCR-聚丙烯酰胺凝胶(PCR-SDS PAGE)技术、PCR-琼脂糖凝胶电泳技术检测线粒体RegionV 9...调查贵州9个世居少数民族线粒体DNA Region V以及Y染色体DYS287位点多态性情况,为贵州相关少数民族的遗传背景研究提供可参考的分子遗传学数据。采用PCR-聚丙烯酰胺凝胶(PCR-SDS PAGE)技术、PCR-琼脂糖凝胶电泳技术检测线粒体RegionV 9 bp序列的缺失、Y染色体DYS287位点多态性。结果显示在本次研究的9个少数民族中线粒体DNA的Region V发现2种多态性,即标准型和缺失性,仡佬族、畲族、壮族、仫佬族、毛南族、蒙古族、回族、羌族、满族线粒体DNA Region V 9 bp缺失频率分别为:26.6%、25%、20%、18.3%、13.3%、10%、8.3%、6.7%、5%;Y染色体DYS287多态显示:仡佬族、仫佬族YAP+携带率分别为20%、1.67%,其余民族全部显示为YAP-。研究获得贵州9个民族人群线粒体DNA Region V及Y染色体DYS287位点多态数据,为各民族群体遗传分析及起源等提供相关遗传背景资料。展开更多
基金supported by the programs of the Strategic Priority Research Program B of the Chinese Academy of Sciences (CAS) (No. XDB31000000)the National Natural Science Foundation of China (Grant No. 31460091)+2 种基金the Na tional Animal Collection Resource Center, China (Grant No. 2005DKA21402)the Application of Amphibian Natural Antioxidant Peptides as Cosmetic Raw Material Antioxidants (QKZYD [2020]4002)the National Top Discipline Construction Project of Guizhou Province,Geography in Guizhou Normal University (No. 85 2017 Qianjiao Keyan Fa)。
文摘The Paramesotriton caudopunctatus species group is mainly distributed in the karst mountain ecosystems of Guizhou, China. Although some species have been included in previous phylogenetic studies, the evolutionary relationships and divergence-time of members of this species group as a whole remain unexplored. In this study, we report the sequencing of one protein coding mitochondrial gene fragment(ND2) and one nuclear gene(POMC), and use a combination of phylogenetic analyses and coalescent simulations to explore the cryptic diversity and evolutionary history of the P. caudopunctatus species group. Phylogenetic relationships revealed that the P. caudopunctatus species group is composed of two major groups, i. e., East Clade and Western-South Clade. The divergence-time and ancestral area estimation suggested that the P. caudopunctatus species group likely originated in the Doupeng Mountains in Guizhou, China at 12.34 Ma(95% HPD: 8.30–14.73), and intraspecific divergence began at about 2.17 Ma(95% HPD: 1.39–2.97). This timing coincides with the orogenesis of the Miaoling Mountains during the Late Miocene to early Pleistocene. The delimitation of species in the P. caudopunctatus species group supports the existence of the currently identified species, and consensus was confirmed across methods for the existence of least to two cryptic species within what has been traditionally considered to be P. caudopunctatus species group. This study is of significance for understanding the species formation, dispersal, and diversity of the tailed amphibians in the karst mountains ecosystem of Guizhou and the role of the Miaoling Mountains as a geographical barrier to species dispersal.
基金supported by the National Natural Science Foundation of China(Grant No.41727801)the Geological Exploration Foundation of Guizhou Province(No.208-9912-JBN-UTSO)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘The widely spread Carboniferous-Permian coal seam group in southern China has great potential for coalbed methane resources,but the extensively developed tectonically deformed coal seriously restricts its development.Taking the Dahebian block in western Guizhou as the study area,the geological model of coalbed methane reservoirs in the tectonically deformed coal seam group was established,and the spatial distribution pattern of model parameters was clarified by clustering algorithms and factor analysis.The facies model suggests that the main coal body structures in Nos.1,4,and 7 coal seams are cataclastic coal and granulated coal,whereas the No.11 coal seam is dominated by granulated coal,which has larger thicknesses and spreads more continuously.The in situ permeability of primary undeformed coal,cataclastic coal,granulated coal,and mylonitized coal reservoirs are 0.333 mD,0.931 mD,0.146 mD,and 0.099 mD,respectively,according to the production performance analysis method.The property model constructed by facies-controlled modeling reveals that Nos.1,4,and 7 coal seams have a wider high-permeability area,but the gas content is lower;the high-permeability area in the No.11 coal seam is more limited,but the gas content is higher.The results of the self-organizing map neural network and K-means clustering indicate that the geological model can be divided into 6 clusters,the model parameter characteristics of the 6 clusters are summarized by data analysis in combination with 6 factors extracted by factor analysis,and the application of data analysis results in multi-layer coalbed methane co-development is presented.This study provides ideas for the geological modeling in the tectonically deformed coal seam group and its data analysis.
文摘调查贵州9个世居少数民族线粒体DNA Region V以及Y染色体DYS287位点多态性情况,为贵州相关少数民族的遗传背景研究提供可参考的分子遗传学数据。采用PCR-聚丙烯酰胺凝胶(PCR-SDS PAGE)技术、PCR-琼脂糖凝胶电泳技术检测线粒体RegionV 9 bp序列的缺失、Y染色体DYS287位点多态性。结果显示在本次研究的9个少数民族中线粒体DNA的Region V发现2种多态性,即标准型和缺失性,仡佬族、畲族、壮族、仫佬族、毛南族、蒙古族、回族、羌族、满族线粒体DNA Region V 9 bp缺失频率分别为:26.6%、25%、20%、18.3%、13.3%、10%、8.3%、6.7%、5%;Y染色体DYS287多态显示:仡佬族、仫佬族YAP+携带率分别为20%、1.67%,其余民族全部显示为YAP-。研究获得贵州9个民族人群线粒体DNA Region V及Y染色体DYS287位点多态数据,为各民族群体遗传分析及起源等提供相关遗传背景资料。