Major Diseases and Pests of Blueberry in Guizhou Province and Progress in Their Prevention and Control

As the blueberry industry continues to evolve,the effective control of its diseases and pests has become an essential component of local agricultural development.This paper provides a comprehensive overview of the principal types of blueberry diseases and pests in Guizhou Province,along with the corresponding control measures,in order to serve as a valuable reference for blueberry growers.

New Macroalgal Fossils from Middle Cambrian Kaili Biota in Guizhou Province, China

Abundant and well-preserved remains of noncalcareous algae and soft-bodied metazoans were collected from Middle Cambrian Kaili biota in Taijiang county, Guizhou Province, China. These remains provide further evidence for the wide geographic distribution of many Burgess Shale taxa. Among the algae, 5 genera (including two new genera) and 5 species are described. They are Marpolia spissa Walcott, Acinocricus stichus Conway Morris and Robison, Udotealga erecta Yang, Eosargassum sawata Yang, and Rhizophyton zhaoyuanlongii Yang. Contrasting the macroalgal fossil assemblage in the Kaili biota with one in the Burgess Shale biota, it is clear that similarity of the Kaili biota and the Burgess Shale biota is reflected by the same content of not only the soft-bodied metazoans, but also the noncalcareous algae.

Diagnosis of Mixed Infection of CSFV, PRV and E.coli in a Modern Pig Farm of Guizhou Province

In order to understand the incidence reasons in a scaled pig farm of Guizhou Province, the infected pigs were diagnosed with epidemiological investigation, clinical observation, pathological diagnosis, enzyme-linked immunosorbent assay （ELISA）, PCR/RT-PCR and bacterial isolation and identification. The results showed that the pigs were infected mixedly with classical swine fever virus （CSFV）, pseudorabies virus （PRV） and E. coli. Therefore, some measures should be adopted to control the incidence of CSF, PR and colibacillosis in the pig farm.

Correlation Between the Animal Community Structure and Environmental Factors in Dongbei Cave and Shuijiang Cave of Guizhou Province

Mollusks, arthropods and chordates which were visible to the naked eye were observed and collected in Dongbei and Shuijiang caves of Libo county five times between February and July from 2002 to 2005. Four hundred and forty samples from Dongbei Cave were classified into three phyla, five classes, 10 orders, 20 families and 39 species or groups of species. Four hundred and ninety-eight samples from Shuijiang Cave were classified into three phyla, six classes, 11 orders, 20 families and 25 species or groups of species. Six animal communities were identified in the light belt of the two caves according to their species types and numbers of individuals in the light belt of two cave. The communities which have the highest values of species richness community diversity, maximum diversity, evenness, dominance and community similarity are respectively： B （4. 1059）, H （2.4716）, B （3.3322）, E （0.9042）, C （0.3442） and A - C （0. 5251）. The community diversity and correlation of environmental factors were also studied. The temperature, humidity, content of CO2 and N2, content of organic matter and some inorganic salts in soil were analyzed by Pearson correlation. The results showed that the content of organic matter in soil is positively related to species number, species richness and maximum community diversity, with correlations of 0. 885, 0.909, 0. 868 respectively （two-tailed significance test, P ≤ 0. 05）, and significantly positively related to diversity, with the coefficient of 0.611, （two-tailed significance test, P ≤ 0. 1）. This suggests that the content of organic matter in soil is one of the important elements influencing the community variation of cave animals. Outside the cave, temperature and humidity are important factors impacting on community diver- sity. As the temperature in all seasons in the cave is stable and the humidity is always high （above 90% ）, the temperature and humidity have weak correlation to community diversity in caves.

The Historical Status of Guizhou Province on Glutinous Rice Cultivation Sphere of China

Guizhou Province is part of the glutinous rice cultivation sphere in our country.Its rice production and germplasm resources play an important role.The southeast region is the centre of the Guizhou glutinous rice cultivation and characterized by waxy wo,which is treasure of the original farming culture of southeast Guizhou region and an important symbol for ethnic cultural identity of the region.

Dynamic Change and Simulation and Prediction of the Karst Rocky Desertification Sensitivity in Guizhou Province

Sensitivity evaluation of rocky desertification is to recognize the areas prone to be rock-deserted,so as to assess the sensitivity degree of rocky desertification to human activities.To perform the sensitivity evaluation of rocky desertification,we should be on the basis of the mechanism of rocky desertification formation for analyzing its regional distribution difference in the sensitivity and further for finding out the rocky desertification-susceptive areas and their possible degrees.Employing remote sensing and geological information system techniques,we regarded the 1∶ 50 000 topographic map and the TM image in 2000 of Guizhou Province,together with the hydrologic map,soil map,geologic map,rock formation map,administrative map and other geological information as data sources to generate the degree data of rocky desertification sensitivity of Guizhou Province in 1962 and 2000,based on which we determined the corresponding degree transfer matrix and adopted Markov model to simulate the dynamic change process of rocky desertification sensitivity and its future evolution trend.In various sensitivity types of rocky desertification in Guizhou Province,only the insensitive type showed a declined acreage,from the proportion of total land acreage of 61.35% in 1962 to 55.55% in 2000.Various sensitivity types inter-conversed,resultantly the acreage of mildly sensitive type increased most.The prediction results showed that the acreage of insensitive type of rocky desertification in Guizhou Province will assume a remarkable decline trend,of mildly sensitive type will present a remarkably increasing trend and of slightly sensitive type basically will maintain a stable level in the future tens of years.Acreages of both highly sensitive and extremely sensitive type of rocky desertification expanded to some extent,suggesting that the rocky desertification risk in Guizhou Province is in an aggravating process.

Human Driving Forces:Analysis of Rocky Desertification in Karst Region in Guanling County,Guizhou Province

Karst rocky desertification is one of the major ecological and environmental problems that threaten the sustainable development of southwestern China. It is caused by irrational and intensive land-use patterns in karst geo-ecological environment. Therefore, it is vital to identify how human forces work on this degraded environment. Based on the soil erosion information in 2000 and remote sensing images of Guanling County collected in 2000 and 2007, four grades of karst rocky desertification data in 14 villages of Guanling County were extracted. Impacts of population, affluence, and other human forces on karst rocky desertification were analyzed using STIRPAT model. The results show that:1) Factors of population and affluence had strong influence on karst rocky desertification. In the STIRPAT model analysis, the population and affluence coefficients were positive, indicating that the increase in population and affluence would lead to more serious desertification. 2) Factors of farmer correlated with karst rocky desertification negatively, especially the way of viewing the relationship between people and nature, and the level of knowledge about rocky desertification. Government behavior was not a significant factor in this analysis. 3) The findings provide evidence that STIRPAT model can be used to analyze the relationship between human driving forces and rocky desertification.

Rocky Desertification Risk Zone Delineation in Karst Plateau Area:A Case Study in Puding County,Guizhou Province

Karst rocky desertification is a geo-ecological problem in Southwest China. The rocky desertification risk zone delineation could be used as a guide for the regional and hierarchical rocky desertification management and prevention. We chose the middle and lower reaches of the Houzhai underground basin on the karst plateau in Puding County, Guizhou Province, China as the study area and selected land use type, elevation, slope, aspect, lithology and settlement buffer as the main driving factors of the rocky desertification. The potential risk of rocky desertification was quantifed with the factor-weights union method and statistical analysis method. Five grades of rocky desertification risk were delineated based on Geographic Information System. The extremely low, low, moderate, high and extremely high rocky desertification risk zones accounted for 5.01%, 44.17%, 33.92%, 15.59% and 1.30%, respectively. As a whole, the rocky desertification risk level was moderate because the area of low and moderate rocky desertification risk zones occupied 78.09% of the study area. However, more than half of the area （about 50.81%） was predicted to have moderate rocky desertification risk and above, indicating that the study area was subject to rocky desertification. Rocky desertification risk was higher in the southeast and lower in the northwest of the study area. Distinct differences in the distribution of rocky desertification risk zones corresponding to different factors have been found.

A New Species of the Asian Toad Genus Megophrys sensu lato(Amphibia: Anura: Megophryidae) from Guizhou Province, China

We describe a new species of the genus Megophrys sensu lato from Guizhou Province, China. Molecular phylogenetic analyses based on mitochondrial DNA and nuclear DNA sequences all strongly supported the new species as an independent lineage in Megophrys(Panophrys) clade. The new species is distinguished from its congeners by a combination of the following morphological characteristics:(1) small body size with SVL < 38.8 mm in male and SVL < 42.3 mm in female;(2) vomerine teeth absent;(3) tongue not notched behind;(4) a small horn-like tubercle at the edge of each upper eyelid;(5) tympanum distinctly visible, rounded;(6) two metacarpal tubercles in hand;(7) relative finger lengths: II < I < V < III;(8) toes with rudimentary webbing at bases;(9) heels overlapping when thighs are positioned at right angles to the body;(10) tibiotarsal articulation reaching the level between tympanum to eye when leg stretched forward;(11) an internal single subgular vocal sac in male;(12) in breeding male, the nuptial pads with black nuptial spines on the dorsal bases of the first and second fingers.

Analysis on Temporal and Spatial Variation Characteristics of Potential Evapotranspiration in Guizhou Province

[Objective] The aim was to analyze temporal and spatial variation charac- teristics of potential evapotranspiration in Guizhou Province. [Method] According to information from 19 meteorological stations of Guizhou Province from 1960 to 2007, with the formulas of optimizational Penman-Monteith and SEBAL net radiation mod- el, the reference crop evapotranspiration ETo was calculated. Also with the spatial interpolation method of Arcgis 9.3, climate tendency rate statistics, K-M test, wavelet analysis and so on, the related regional differentiation and spatial and temporal change characteristics between meteorological factors from each monitoring station and ETo were analyzed. [Result] The results show that the correlation of potential evapotranspiration and meteorological factors in Guizhou Province demonstrates re- gional differentiation; there was no direct connection between the correlation of po- tential evapotranspiration and meteorological factors and the amount of meteorologi- cal factors in the year; there were 3 cycles in potential evapotranspiration, namely, 1 year, 5 years and 10 years, with 3 mutation points, respectively, in 1965, 1984 and 1999, mainly affected by air temperature, precipitation and solar radiation. [Conclusion] The research is of great significance in developing irrigation approach- es, adjusting agricultural structure and ecological construction.

Organic Geochemistry of Sedimentary Rock-hosted Disseminated Gold Deposits in Southwestern Guizhou Province, China

Sedimentary rock-hosted disseminated gold (SRHDG) deposits in the Youjiang-Nanpanjiang Basin, southwestern Guizhou Province are commonly hosted by the same fold crests that commonly contain a remarkable amount of organic material. The total organic carbon (TOC) contents of the ores and host rocks are usually less than 1%. The reflectance of vitrinite and pyrobitumen in the ores and the host rocks ranges from 1.5% to 4.5%, often in the range of 2% to 3%. In the Lannigou deposit, the reflectance of vitrinite and pyrobitumen in the ores is usually somewhat higher than those within the host rocks, indicating a hydrothermal impact on the organic matter in the altered host rocks. On the contrary, the estimated maximum paleotemperatures of the Getang and Zimudang deposits are higher than the homogenization temperatures of the fluid inclusions in the ores, signifying that the organic matter maturation predated Au mineralization. No correlation between the organic matter contents and Au concentrations were recognized in the ores. However, the most striking observation is that there is a positive correlation between the S2 (a parameter of Rock-Eval analysis), Au and As contents of the ores in the Lannigou deposit. Organic matter maturation and migration is apparent from the TOC vs. HCI diagram. Furthermore, group analysis of the dichloromethane extractable organic component of the ores and host rocks shows that the maturation degree of the organic matter in the ores is slightly higher than that of the host rocks in the Lannigou gold deposit. However, the compositions of their alkanes, steranes and terpenes, which serve as biomarkers, are quite similar; this suggests that the organic matter found in the ores and host rocks has a common marine source. Organic matter probably contributed to the preconcentration of Au in the host rocks. Hydrocarbons in the system, on the other hand, clearly contributed to the emplacement of the gold mineralization through thermal sulfate reduction. Organic matter in the solution might have increased the potential of the hydrothermal solution to transport Au.

Quality Characteristics of Tobacco Leaves with Different Aromatic Styles from Guizhou Province,China

The relationships between chemical components and quality indexes were studied in the tobacco leaves with different aromatic styles. A total of 16 chemical components, 4 quality indexes, and 6 smoking quality indexes from 366 tobacco leaf samples with 4 different types of aroma from Guizhou Province, China, were subjected to principal component analysis and stepwise regression analysis. The tobacco leaves with different types of aroma showed remarkable difference in the contents of chemical components, quality indexes, and smoking quality indexes. The first principal factors （carbohydrates and nitrogen-containing compounds） of the chemical composition of the leaf were similar among different types of aroma, which showed that the quality of the leaf was mainly influenced by carbohydrates and nitrogen-containing compounds and their ratios. The factors for the second through the seventh principal components varied largely among varioUs aromatic types, suggesting the contribution of other chemical components to the leaf quality. In addition, the smoking quality of four different aromatic leaves showed significant correlation with the different chemical components. The quality of tobacco leaves with different types of aroma was influenced by multiple factors, especially ecological conditions and culture techniques, which may provide guidance for directive cultivation of high-quality tobacco leaves.

Emplacement Ages and Geochemical Characteristics of Grabbroic Intrusions and Prospecting Orientation of Related Deposit in Luodian,Guizhou Province

Emplacement ages, geochemical characteristics and analysis of continental dynamics on gabbroic intrusions in Luodian County, Guizhou Province, have been discussed based on studies of isotopic chronology （the whole-rock Sm-Nd and Rb-Sr isochron methods）, major elements, trace elements and PGE. Intrusive activities of the gabbroic intrusions in the study area took place during the Late Yanshanian Orogenic Movement （the Cretaceous Period）, as indicated by the Sm-Nd isochron ages （t）=（99.6±4.5） （2σ） Ma and by the Rb-Sr isochron ages t=（97±1.6） （2σ） Ma. The gabbroic intrusions are attached to mafic rocks in cal-alkaline basaltic series. They occurred as dikes and might be formed under an extensional background of the continent. Differentiation of their magmatic crystallization resulted in obvious zonation of petrography. In the gabbroic intrusions of this study, large ion lithophile elements and LREE are enriched, and the chondrite-normalized REE distribution pattern is leftward inclined without anomalies of JCe or JEu, and there are high concentrations of PGE and ratios of Pd/Ir （averaging 4.21）. All of these imply that their source areas may be basaltic magma in the upper mantle with high-level partial melting, derived from EMl-type enriched mantle. It is different from Emeishan basalt, which may be related to the upper mantle at low-grade partial melting. Emplacement mechanism of the gabbroic intrusions in this study may suppose to be asthenosphere upheaving as an isolated hot wave in the presence of mantle fluid, resulting in basaltic magma intruded into the continental crust as a diapiric intrusion. Therefore, uplifting of faulting-block and extensional deformation could take place in the shallow part of the continental crust while vertical amassing and accretion of magmatic materials in the deep part of the continental crust. These special processes could supposed to be a special background of continental dynamics for this large-scale epithermal metallogenic domain, such as Au, As, Sb and Hg deposits, and vertical uplifting of the Yunnan-Guizhou Plateau in the Cretaceous Period.

The Discovery of a Crinoid Community in the LateTriassic Zhuganpo Formation of Guizhou Provinceand Its Geological Significance

: Recently a rich and well—preserved crinoid community including Traumatocrinus and Encrinus has been found in the Late Triassic Zhuganpo Formation in Guanling and Xingyi, Guizhou province, China. Among the fossils Traumatocrinus is the richest, and most of it occurs as clusters, with each cluster containing 3–42 crinoid branches. Study of the stem and calyx of Traumatocrinus shows that the number of stem-joints (columnals) is equal to the total stem length × K (5.85 per cm × stem—diameter). There are about 376 first- and second—order columnals on the whole stem. This number seems to coincide with the number of days in a year at that time. According to the present study of the palaeoecological environment of the crinoid community, it is considered that the reproduction and preservation of the crinoid community were controlled by the Late Triassic regression and the restricted bay of an interior sea behind the S—shaped shoal zone.

Spatial-temporal Changes of Vegetation Cover in Guizhou Province, Southern China

Guizhou Province is an important karst area in the world and a fragile ecological area in China. Ecological risk assessment is very necessary to be conducted in this region. This study investigates different characteristics of the spatial-temporal changes of vegetation cover in Guizhou Province of Southern China using the data set of SPOT VEGETATION(1999–2015) at spatial resolution of 1-km and temporal resolution of 10-day. The coefficient of variation, the Theil-Sen median trend analysis, and the Mann-Kendall test are used to investigate the spatial-temporal change of vegetation cover and its future trend. Results show that: 1) the spatial distribution pattern of vegetation cover in Guizhou Plateau is high in the east whereas low in the west. The average annual normalized difference vegetation index(NDVI) from west to east is higher than that from south to north. 2) Average annual NDVI improved obviously in the past 17 years. The growth rate of average annual NDVI is 0.028/10 yr, which is slower than that of vegetation in the country(0.048/10 yr) from 1998 to 2007. Average annual NDVI in karst area is lower than that in non-karst area. However, the growing rate of average annual NDVI in karst area(0.030/10 yr) is faster than that in non-karst area(0.023/10 yr), indicating that vegetation coverage increases more rapidly in karst area. 3) Vegetation coverage in the study area is stable overall, but fluctuates in the local scales. 4) Vegetation coverage presents a continuous increasing trend. The Hurst exponent of NDVI in different vegetation types has an obvious threshold in various elevations. 5) The proportion of vegetation cover with sustainable increase is higher than that of vegetation cover with sustainable decrease. The improvement in vegetation cover may expand to most parts of the study area.

The importance of non-carbonate mineral weathering as a soil formation mechanism within a karst weathering profile in the SPECTRA Critical Zone Observatory, Guizhou Province,China

Soil degradation, including rocky desertification,of the karst regions in China is severe. Karst landscapes are especially sensitive to soil degradation as carbonate rocks are nutrient-poor and easily eroded. Understanding the balance between soil formation and soil erosion is critical for long-term soil sustainability, yet little is known about the initial soil forming processes on karst terrain. Herein we examine the initial weathering processes of several types of carbonate bedrock containing varying amounts of non-carbonate minerals in the SPECTRA Critical Zone Observatory, Guizhou Province, Southwest China. We compared the weathering mechanisms of the bedrock to the mass transfer of mineral nutrients in a soil profile developed on these rocks and found that soil formation and nutrient contents are strongly dependent upon the weathering of interbedded layers of more silicate-rich bedrock(marls). Atmospheric inputs from dust were also detected.

Metallogenic Model of Bauxite in Central Guizhou Province:an Example of Lindai Deposit

The bauxites in central Guizhou are hosted by the Lower Carboniferous Jiujialu Formation.Geochemistrial characteristics of the Lindai bauxite deposit indicate that the underlying Shilengshui Formation dolomite is the precursor rock of mineral resources.Weathering simulation experiments show that Si is most likely to migrate with groundwater,the migration rate of which is several magnitude higher than Al and Fe under nature conditions （pH=3-9）.The neutral and acid nonreducing condition is the most conducive to the Al rich and Si removal,while the acid reducing conditions is the most conducive to the Al rich and Fe removal.In the process of bauxite formation,coal beds overlying the Al-bearing rock series or other rock formation rich in organic materials can produce acid reducing groundwater,which are important for the bauxite formation.Finally,propose the metallogenic model of the bauxite in central Guizhou Province and put forward three new words which are ＂original bauxite material＂,＂bauxite material＂ and ＂original bauxite＂.

GIS-based spatial differentiation of ethnic minority villages in Guizhou Province,China

The protection and development problems in ethnic minority villages have received great attention from the Chinese government.This study aims to systematically identify the spatial differentiation characteristics of ethnic minority villages and their influencing factors in Guizhou Province.Applying ArcGIS and GeoDa,a total of 1,014 ethnic minority villages in the four batches announced by the Ethnic and Religious Affairs Commission of Guizhou Province(272in the first batch,240 in the second batch,255 in the third batch,and 247 in the fourth batch)served as the research objects.Utilizing the economic development data in Guizhou Statistical Yearbook 2018-2020 for analysis,the coupling calculation of the spatial layout and economic development level of ethnic minority villages was conducted.The results indicate that the number of ethnic minority village points varies in spatial distribution with remarkable agglomeration.The spatial equilibrium distribution of ethnic minority villages in the province is imbalanced.The kernel density is characterized by independent single kernel centers with sporadic distributions of subcenters and an echelon distribution whole.The spatial complexity distribution indicates that ethnic minority villages in Guizhou possess unequal characteristics,and the fractal of the system structure is more complicated.The findings reveal that the spatial distribution of ethnic minority characteristic villages in Guizhou is highly consistent with that of ethnic minorities in Guizhou.The distribution is the combined results of geographical factors and socialeconomic factors.Most ethnic minority characteristic villages in Guizhou Province are located in areas with complex terrain,inconvenient transportation,and relatively backward economic development.Based on the current geospatial distribution of ethnic minority villages,the present study will provide remarkable implications for the Chinese government to implement the Rural Revitalization Strategy from several key aspects.The Chinese government should establish a protection and development mechanism in ethnic minority villages and strive to enhance the characteristics of ethnic cultural industries.Meanwhile,the"integration of culture and tourism"should be taken as the development strategy while the industry should support rural economic development and help eliminate poverty.This is of crucial practical significance to national unity and rural revitalization in China.

Climatic Characteristics of Hail Disaster and Division of Defense Region in Guizhou Province

[Objective] The aim was to study the climatic characteristics of hail disaster from 1984 to 2006 in Guizhou Province,China.[Method] Through statistical analysis,the climatic characteristics and disaster characteristics of hail from 1984 to 2006 in Guizhou Province were annalyzed and compared with previous results,then the classification standards of hail disaster were established,finally the defense zoning map of hail disaster in Guizhou Province was made.[Result] According to the statistical analysis on the historical data of hail disaster from 1984 to 2006,the frequency hail was 1 243 times in Guizhou in 23 years,that is,annual average frequency reached 54 times;annual average hailing days were 0.1-2.7 d in Guizhou Province,and the average frequency of hail was 3.8 times during each hail process;the frequency of hail was below 30 times in 52% of years and 30-80 times in 35% of years among 23 years.Hail could appear in the whole year,and 74% of hails occurred in spring,while winter had the fewest times.The results above were basically consistent with those of previous studies.Hail disaster mainly resulted in casualties and direct economic loss,and was divided into four grades according to direct economic loss,namely Grade I(extremely severe hail disaster),Grade II(severe hail disaster),Grade III(comparatively severe hail disaster) and Grade IV(medium hail disaster).The defense zoning map of hail disaster in Guizhou Province was made based on the frequency of hail and severe hail disaster,that is,the defense region at Grade I mainly distributed in Liupanshui City(in western Guizhou) and southern Guizhou;the defense region at Grade II was in the west,south and northeast of Guizhou Province,while the rest was at Grade III.[Conclusion] The study was of reference value for the defense of hail disaster in Guizhou Province.

Risk factors for pertussis among children hospitalized for pertussis during 2016-2017, in Guizhou Province of China: a case-control study

Aim:Pertussis is a respiratory tract infection,the vaccine for which was introduced in the Expanded Programme on Immunization(EPI)in Guizhou Province of China(hereafter referred as Guizhou)in the 1980s.This vaccine rapidly decreased incidence rates of pertussis in the province,however,despite the wide high coverage of the diphtheria,tetanus and acellular pertussis(DTaP)combined vaccine,there has been a resurgence of pertussis since 2014.Even with this recent increase in disease transmission,risk factors for pertussis infection have not been evaluated in Guizhou.We aimed to provide information on pertussis risk factors and insight on designing targeted pertussis control policies and measures through this study.Methods:A 1:2 matched retrospective case-control study was conducted between 2016 and 2017,involving infants and children younger than 6 years old and parents of the participants.The enrolled cases included clinical and laboratory confirmed pertussis cases according to the WHO-recommended pertussis definition.Controls were selected from children in the same neighborhood who were not diagnosed with pertussis prior to our investigation and did not exhibit any clinical manifestations of pertussis.Results:The household size[Matched odds ratio(OR_(m)=1.4,95%confidence interval(Cl):1.1-1.7]and household members with antecedent cough(OR_(m)=3.6,95%CI:1.8-7.2)were significantly associated with their child’s pertussis onset.The parents’occupations were significantly associated with their child’s pertussis onset(ORm=9.4,95%CI:1.6-54.8,for mother side;ORm=4.5,95%CI:1.2-16.5,for father side),when they worked in the business and/or service industry.Having family members with a history of cough was an independent risk factor for pertussis[Adjusted odds ratio(AOR)=43.6,95%CI:2.7-694.0].Besides,the parents’demographic characteristics and DTaP doses were not found to be independent factors.Conclusion:Household exposure is an important risk factor for pertussis infection in infants and young children and should therefore be considered as a major factor during formulation of pertussis control policies and measures.