The abundance,distribution,and enrichment mechanism of harmful trace elements in coals from Guizhou,Southwestern China

Coal seams can enrich a variety of harmful trace elements under specific geological conditions.The spatial distribution of harmful trace elements in coal is extremely uneven,and the distribution characteristics of each element content are different.The harmful elements released in the process of coal mining and utilization will cause serious harm to the environment and the human body.It is of great resource significance to study the geochemistry of coal that affects the enrichment and distribution characteristics of harmful trace elements.Based on the domestic and foreign literature on coal geochemistry in Guizhou published by previous investigators,this study counted 1097 sample data from 23 major coal-producing counties in Guizhou Province,systematically summarized the relevant research results of harmful trace elements in the coal of Guizhou,and revealed the overall distribution and enrichment characteristics of harmful trace elements in the coal of Guizhou.The results show that the average contents of Cd,Pb,Se,Cu,Mo,U,V,As,Hg,and Cr in coal of Guizhou are higher than those in Chinese coal and world coal.A variety of harmful trace elements in the coal of Guizhou have high background values,especially in Liupanshui,Xingyi and Qianbei coalfield.The enrichment of various harmful trace elements in the Late Permian coal in Guizhou is mainly related to the combined action of various geological and geochemical factors.The supply of terrigenous debris and sedimentary environment may be the basic background of the enrichment of harmful elements in western Guizhou,while low-temperature hydrothermal activity and volcanic ash deposition may be the main reasons for the enrichment of harmful elements in southwestern Guizhou.

Ancient Zhenyuan, Gateway to East Guizhou

This ancient city has a history going back over 2,000 years.It has witnessed the fusion of multiple ethnic cultures including the Miao,Dong,and Han,and hosts a number of cultural heritage sites.THE old town of Zhenyuan is located in Zhenyuan County,Qiandongnan Miao and Dong Autonomous Prefecture in southwest China’s Guizhou Province.Covering an area of just around three square kilometers,it sits on the banks of the Wuyang River and is surrounded by mountains.Zhenyuan has been a county for more than 2,281 years and was the prefectural capital during the Yuan(1271-1368)and Qing(1644-1911)dynasties.It is a multicultural region,with Miao,Dong,and other ethnic minorities making up nearly half of the population.

Chinese New Year Folk Culture Adds Impetus to Guizhou's Colorful Tourism Economy

During the Chinese New Year in 2024,a series of traditional folk activities were held all over Guizhou,attracting a large number of travelers,with the number of received travelers and comprehensive income of Guizhou reaching a new high.

Major Diseases and Pests of Blueberry in Guizhou Province and Progress in Their Prevention and Control

As the blueberry industry continues to evolve,the effective control of its diseases and pests has become an essential component of local agricultural development.This paper provides a comprehensive overview of the principal types of blueberry diseases and pests in Guizhou Province,along with the corresponding control measures,in order to serve as a valuable reference for blueberry growers.

伪狂犬病病毒Guizhou-DY株的分离鉴定及致病性研究

通过采集疑似猪伪狂犬病流产胎儿病料，采用细胞接毒分离培养、病毒蚀斑纯化、病毒的形态结构观察、易感动物接种试验和病毒核酸鉴定分离到1株伪狂犬病病毒（PRV），命名为PRVGuizhou—DY株（GenBank登录号为JX417716）；为了摸清其理化特性，进一步对Guizhou—DY分离株进行了耐热、耐酸及脂溶性敏感性试验、核酸类型鉴定及病毒毒价测定，同时进行病毒致病性试验。分离鉴定结果表明：病料接种Vero细胞盲传至第2代时产生明显CPE，盲传至第4代可出现稳定的CPE；病毒粒子在电镜下呈椭圆形、有囊膜、直径为120～180nm，核内可见呈晶格状排列的病毒包涵体；病毒悬液接种家兔能引起家兔奇痒、麻痹死亡；理化特性研究表明：该病毒是一种耐热不耐酸且对氯仿敏感的DNA病毒，gE全基因序列与PRVGZ—Z1株、Fa株及Ea株的相似性分别为97．60A、98．9％和99．4％，其TCID50为10-9.71·100μL-1，PFu为109·mL-1. 攻毒组猪体温升高，临床症状较为明显，在攻毒后第5天开始出现中和抗体，第21天达到最高。该研究可为PRV病原学研究提供参考。

Influence of Diversity of Prototypical Ethnic Culture in Diversity of Glutinous Rice in Southeast of Guizhou

There were quite abundant prototypical ethnic cultures and plant diversity in Qiandongnan （ Southeast of Guizhou） ,where the primitive natural ecology was also perfectly conserved. It was honored by World Native Culture Fund as One of the Eighteen Ecological and Cultural Conservations in the World. The prototypical ethnic culture of Southeast of Guizhou, as a stable life-style and economic culture type of the local people, came into being in the process of the local minorities＇ communicating, blending and struggling against the natural environment in the long course of history. It could be seen from this research that there were our preliminary research that there are quite abundant resources in Southeast of Guizhou in terms of prototypical ethnic cultures and genetic diversity of glutinous rice. A full reflection of the culture of Glutinous Rice could be seen in the local minority people＇s daily life, production, custom, religious belief, etc. These showed that the formation and great vitality of the genetic diversity of glutinous rice was not only determined by the diversity of local prototypical ethnic cultures but also to its own biotic condition and its adaptation to natural ecology. It was showed that prototypical ethnic cultures had positive impact on the conservation and utilization of Glutinous Rice diversity.

Resources of Wild Ornamental Ferns in Guizhou Province and Their Utilization

Through investigating the wild ornamental ferns in Guizhou Province, it is found that there are totally 312 species (including variations), 111 genera and 49 families of such resources in this region, which are classified into terrestrial fern, epiphytic fern, saxicolous fern, vine fern, tree fern and aquatic fern according to their ecological types. The application of ornamental ferns in garden landscaping and development countermeasures are analyzed in this study.

New Macroalgal Fossils from Middle Cambrian Kaili Biota in Guizhou Province, China

Abundant and well-preserved remains of noncalcareous algae and soft-bodied metazoans were collected from Middle Cambrian Kaili biota in Taijiang county, Guizhou Province, China. These remains provide further evidence for the wide geographic distribution of many Burgess Shale taxa. Among the algae, 5 genera (including two new genera) and 5 species are described. They are Marpolia spissa Walcott, Acinocricus stichus Conway Morris and Robison, Udotealga erecta Yang, Eosargassum sawata Yang, and Rhizophyton zhaoyuanlongii Yang. Contrasting the macroalgal fossil assemblage in the Kaili biota with one in the Burgess Shale biota, it is clear that similarity of the Kaili biota and the Burgess Shale biota is reflected by the same content of not only the soft-bodied metazoans, but also the noncalcareous algae.

Genetic Diversity Analysis of Three Guizhou Local Pig Breeds Using Microsatellite DNA Markers

[Objective] The research aimed to provide the genetic basis for the protection, development and utilization of Guizhou local pig breeds. [Method] From 27 pairs of porcine microsatellite primers recommended by Food and Agriculture Organization of the United Nations （FAO） and international Society for Animal Genetics （ISAG）, six pairs （S0155, SW240, IGF1, SW951, SW857, SW24） were selected for microsatellite DNA detection of three Guizhou local pig breeds, including Nuogu Pig, Kele Pig and Guanling Pig. Subsequently, their genetic diversities were analyzed. [ Result] The three pig breeds were high polymorphic at the six microsatellite loci （PIC 〉0.5）. The Nei＇s standard genetic distance of them was 0.206 3 -0.481 5. The genetic distance between Nuogu Pig and Kele Pig Was the closest, and that between Nuogu Pig and Guanling Pig was the furthest. [ Conclusion] The three Guizhou local pig breeds are in high genetic diversities. Nuogu Pig is a special type of Kele Pig, an excellent Chinese local pig breed.

Temporal and Spatial Distribution Characteristics of Potential Evapotranspiration and Its Sensitivity to Meteorological Factors in Guizhou Province

[Objective] The study aims to discuss the changes of potential evapotran- spiration and its sensitivity to meteorological factors in Guizhou Province, so as to provide important references for assessment of water resources, research of agri- cultural water conservancy and climate change. [Method] Temporal and spatial dis- tribution characteristics of potential evapotranspiration in Guizhou Province from 1961 to 2010 were analyzed, and the sensitivity of potential evapotranspiration to meteo- rological factors in Guizhou Province was studied through correlation analysis. [Re- sult] On the whole, potential evapotranspiration in Guizhou Province was higher in the southwest and the west compared with the northeast and the east. In various seasons, it was the highest in summer, followed by spring and autumn, while it was the lowest in winter. In recent 40 years, annual potential evapotranspiration showed an obvious decreasing trend in most stations of Guizhou Province. The main meteo- rological factors influencing changes of potential evapotranspiration in Guizhou Province were sunshine duration, daily maximum temperature, and daily average relative humidity. [Conclusion] Daily average temperature was not the main meteoro- logical factor affecting changes of potential evapotranspiration in Guizhou Province, while sunshine duration, daily maximum temperature, and daily average relative hu- midity had important effects on potential evapotranspiration in Guizhou Province.

Correlation Between the Animal Community Structure and Environmental Factors in Dongbei Cave and Shuijiang Cave of Guizhou Province

Mollusks, arthropods and chordates which were visible to the naked eye were observed and collected in Dongbei and Shuijiang caves of Libo county five times between February and July from 2002 to 2005. Four hundred and forty samples from Dongbei Cave were classified into three phyla, five classes, 10 orders, 20 families and 39 species or groups of species. Four hundred and ninety-eight samples from Shuijiang Cave were classified into three phyla, six classes, 11 orders, 20 families and 25 species or groups of species. Six animal communities were identified in the light belt of the two caves according to their species types and numbers of individuals in the light belt of two cave. The communities which have the highest values of species richness community diversity, maximum diversity, evenness, dominance and community similarity are respectively： B （4. 1059）, H （2.4716）, B （3.3322）, E （0.9042）, C （0.3442） and A - C （0. 5251）. The community diversity and correlation of environmental factors were also studied. The temperature, humidity, content of CO2 and N2, content of organic matter and some inorganic salts in soil were analyzed by Pearson correlation. The results showed that the content of organic matter in soil is positively related to species number, species richness and maximum community diversity, with correlations of 0. 885, 0.909, 0. 868 respectively （two-tailed significance test, P ≤ 0. 05）, and significantly positively related to diversity, with the coefficient of 0.611, （two-tailed significance test, P ≤ 0. 1）. This suggests that the content of organic matter in soil is one of the important elements influencing the community variation of cave animals. Outside the cave, temperature and humidity are important factors impacting on community diver- sity. As the temperature in all seasons in the cave is stable and the humidity is always high （above 90% ）, the temperature and humidity have weak correlation to community diversity in caves.

Diagnosis of Mixed Infection of CSFV, PRV and E.coli in a Modern Pig Farm of Guizhou Province

In order to understand the incidence reasons in a scaled pig farm of Guizhou Province, the infected pigs were diagnosed with epidemiological investigation, clinical observation, pathological diagnosis, enzyme-linked immunosorbent assay （ELISA）, PCR/RT-PCR and bacterial isolation and identification. The results showed that the pigs were infected mixedly with classical swine fever virus （CSFV）, pseudorabies virus （PRV） and E. coli. Therefore, some measures should be adopted to control the incidence of CSF, PR and colibacillosis in the pig farm.

Construction of Tourism Development Mode for Guizhou Province Based on Sustainable Development

External environment for rural tourism development in Guizhou Province was analyzed from the perspectives of social economy,culture and ecology,and a sustainable mode was constructed.

Analysis of Grassland Productivity and Carrying Capacity of Natural Warm-temperature Tussock in the Central Region of Guizhou Province

[Objective] This study aimed to investigate the influences of grazing and fencing on grassland productivity and carrying capacity of subtropical natural warmtemperature tussock. [Method] With the natural warm temperature tussock in Longli County of Guizhou Province as study area, monthly, seasonal and annual dynamics of grassland productivity were investigate continuously during 2010 -2012, and the data were analyzed. [Result] Under grazing and fencing conditions, grassland present biomass and forage growth of warm temperature tussock in Longli County of Guizhou Province both increased first and decreased, reaching the maximum from August to October. Fencing significantly improved the productivity of natural grassland but had little effect on the vegetation composition. [Conclusion] From the perspective of grass-livestock balance, the suitable stocking capacity of warm-temperature tussock in the central region of Guizhou Province was 3.45 -4.66 sheep/hm2 , which has high ecological efficiency and can be borne by farmers and herdsmen.

Tourist Products Design of National Village Based on Experience——A Case Study of Minority Villages of Southeast Guizhou,China

On the basis of resources features of minority villages in Southeast Guizhou and tourists' experience needs,the paper proposed that national villages would increasingly be the ideal destination of cultural experience tourism under the background of experience economy,and products design centering on experience need was the common problem confronted tourism development of national villages.Through analyzing four aspects of tourist resources which were natural ecology and pastoral scenery,tangible national culture,process and event national culture,and intangible culture,the paper proposed a general method for design of tourist products of national villages.The design should be conducted by following steps of "theme extraction—image shaping—scene design—atmosphere creation—program arrangement—implication design—publicity marketing".It should establish brand image of tourist products of national villages,so as to help tourists realize "image establishment—purchasing impulse—participation into interaction—profound experience—expectation achievement",and to constantly increase the attraction power of tourist products of national villages and comprehensive economic benefit of tourism.

The Historical Status of Guizhou Province on Glutinous Rice Cultivation Sphere of China

Guizhou Province is part of the glutinous rice cultivation sphere in our country.Its rice production and germplasm resources play an important role.The southeast region is the centre of the Guizhou glutinous rice cultivation and characterized by waxy wo,which is treasure of the original farming culture of southeast Guizhou region and an important symbol for ethnic cultural identity of the region.

伪狂犬病病毒Guizhou-T1株的分离鉴定及其遗传变异研究

为了解贵州猪伪狂犬病病毒(PRV)流行株的病原形态特征、致病性及遗传变异情况,本实验对收集自贵州规模化猪场伪狂犬病净化过程中PCR检测的阳性病料样品,采用细胞接毒分离培养、病毒的形态结构观察和易感动物接种试验对其病原进行鉴定。结果显示,本研究分离到一株PRV,命名为PRV Guizhou-T1株;并对其进行病毒效价的测定及g E基因核苷酸和氨基酸序列差异性分析。结果显示:PRV Guizhou-T1株gE基因序列与GZ-Z1株和Guizhou-DY株同源性分别为97.5%和99.3%,其TCID_(50)为10^(-10.11)/100μL;PRV Guizhou-T1株在gE基因编码的氨基酸序列第48和第496位均有天冬氨酸(Asp D)的插入,与PRV变异株变异位点一致,且在其第48位缺失酪氨酸(Tyr Y)。表明本研究分离得到了一株PRV变异株。本研究可为贵州PRV病原学的研究提供参考。

Genetic Polymorphism of Mitochondrial DNA in Dong,Gelao,Tujia,and Yi Ethnic Populations from Guizhou,China

To reveal the genetic structures and relationships of the four ethnic populations from the maternal inheritance and explore the origins and migrations of nationalities, the genetic polymorphism of mtDNA in Dong, Gelao, Tujia, and Yi populations from Guizhou was studied by direct sequencing of hypervariable segment Ⅰ （HVS Ⅰ ） and PCR-RFLP of coding region. Thirty-seven （sub-） haplogroups were identified in the classification tree of mtDNA haplogroups. Haplogroup distributions and principal component （PC） analysis showed that the Dong has high frequencies of south-prevalent haplogroups, which indicates that it is a typically southern population. The Yi harbors high frequencies of the south-prevalent and northern-prevalent haplogroups, which demonstrates that it inherits the maternal characteristics from both southern and northern populations. The Yi and Gelao cluster together, the reason for which might be that their ancestries frequently underwent gene exchanges and mixtures.

Discussion on Production Situation of Main Crops and Related Issues in Guizhou from 1949 to 2008

[Objective]In order to get acquainted with the history and situation of agricultural production in Guizhou Province and find out the differences.[Method]The planting area,production and the yield per unit area of rice,wheat,maize,potato,soybean,rape and tobacco in Guizhou Province from 1949 to 2008 were reviewed to discuss initially on some issues of agricultural production in Guizhou Province.[Result]Planting area and production of rice,wheat and maize were reduced in recent years,the yield per unit area of the main crops were in low level,the area of cultivated land declined year by year with poor quality,the population grew excessively,annual average net income of peasants were low,and the water resources in rural area were low in utilization.[Conclusion]The research provided reference and consult for solving problems of agriculture,rural areas and farmers.

Resource Distribution and Exploitation of Edible Ferns in Guizhou

64 species （including 5 varieties）, 30 genera and 24 families of the edible ferns in Guizhou are preliminartiy confirmed, according to the combination of spot investigation and refereuce analySis：the species, genera and families account for 8.13%, 19.74% and 44.44% of ferns in Guizhou, respectively. The floristic composition characters and resource distribution of 23 kinds of the edible ferns in Guizhou are described.