PCR-RFLP Polymorphism of ANGPTL4 Gene in Guizhou Yellow Cattle

[ Objective] The paper was to study the genetic effects of ANGPTIA gene in Guizhou yellow cattle. [ Method ] The polymorphisms of ANGPT/A gene in 46 individuals of Guarding yellow cattle and Sinan yellow cattle were detected using PCR-RFLP assay. [Result] A fragment of 260 bp was amplified from AN- GPTIA gene. After digestion by restriction endonuclease MluCl, two genotypes, AA and AB, were produced whereas BB genotype was not found. A mutation of 205T→C was observed through sequencing of different genotypes. AA genotype was dominant in both yellow cattle breeds, and A allele frequency was higher than B allele frequency. X2 test results showed that the two cattle breeds were at Hardy-Weinberg equilibrium state in the digestion site （ P 〉 0.05 ）. [Condusion] The effective molecular markers of Guizbou yellow cattle, such as growth trait and intramuscular fat content, are obtained, which will provide a theoretical basis for mo- lecular breeding of meat performance of Guizhou yellow cattle.

Polymorphism of POMC Gene in Guizhou Yellow Cattle

[ Objective ] The paper was to explore the effect of pro-epiomelanocortin （POMC） on feeding behavior and energy homeostasis of animals. [ Method ] Using DNA sequencing technology, polymorphisms of POMC gene in 92 individuals of Guizhou yellow cattle were studied. [ Result ] Five mutation sites, 138 C 〉 T, 430 C 〉 T, 644 C 〉 T, 668C 〉 T, 693A 〉 G, were found in the 870 bp amplification product. Polymorphism results showed that except the mutation site 430 C 〉 T that was deviated from the Hardy-Weinberg equilibrium （HWE） state （ P 〈 0.05 ）, the rest mutation sites were at the HWE state （ P 〉 0. 05 ） ; the peptide infor- mation content （PIC） of five mutation sites were moderate polymorphism. [ Conclusion] The POMC gene of Guizhon Yellow Cattle was abundant in genetic diversity, with great selection potential, which could be used as a potential genetic marker of cattle growth traits.

Improvement Effects of Guizhou Local Yellow Cattle Crossbred with Fleckvieh

[Objective] The aim was to discuss the improvement effect of Guizhou local yellow cattle crossbred with Fleckvieh. [Method] With Fleckvieh as male parent, they were crossbred with Guizhou local yellow cattle （ ♀ ）, and hybridized cattle of Simmental × local yellow cattle （ ♀ ） to determine production performance, slaughter performance and lactation performance of their offspring. And they were compared with Guizhou local yellow cattle. [ Result ] The growth, slaughter and lactation performance of offspring of F1 Fleckvieh （ ♂ ） × Simmental （ ♂ ） × local yellow cattle （ Q ） proved optimal, followed by offspring of F1 Fleckvieh （ ♂ ） × local yellow cattle （ ♀） and offspring of Guizhou local yellow cattle. [ Conclusion] Offspring of improved local yellow cattle crossbred with Fleckvieh （ ♂ ） and hybridized cattle of Fleckvieh × Simmental × local yellow cattle enjoy significant heterosis and the economic benefits of cattle farming could be significantly enhanced.