[Objectives]To accurately determine the naringin content in Rhizoma Drynariae.[Methods]The high performance liquid chromatography(HPLC)method was applied in the determination of the naringin content in Rhizoma Drynari...[Objectives]To accurately determine the naringin content in Rhizoma Drynariae.[Methods]The high performance liquid chromatography(HPLC)method was applied in the determination of the naringin content in Rhizoma Drynariae.The sample was sonicated at room temperature.The mobile phase was 0.1%phosphoric acid-acetonitrile(75∶25),detected by diode array detector at the wavelength of 284 nm,and quantified by external standard method.[Results]The linearity of naringin was good in the concentration range of 5-500μg/mL with a correlation coefficient of 0.9999.[Conclusions]This method has good linearity,easy operation,correctness and reproducibility as required,and is expected to provide a method for the determination of naringin content in Rhizoma Drynariae.展开更多
<strong>Objective:</strong> To investigate the potential mechanism of Drynariae Rhizoma-Epimedii Folium in the treatment of osteoarthritis (OA) based on network pharmacology. <strong>Methods:</str...<strong>Objective:</strong> To investigate the potential mechanism of Drynariae Rhizoma-Epimedii Folium in the treatment of osteoarthritis (OA) based on network pharmacology. <strong>Methods:</strong> The potential active constituents and targets of Drynariae Rhizoma-Epimedii Folium were screened through the traditional Chinese medicine (TCM) systems pharmacology database and analysis platform (TCMSP). Genecards database is used to find relevant targets of OA. The targets of “Drynariae Rhizoma-Epimedii Folium” were mapped to the targets of OA, and used Cytoscape software to build a “drug-ingredient-target-di- sease” regulatory network and protein protein interaction (PPI) network. R software was used to analyze the Gene ontology (GO) function and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment of traditional Chinese medicine-disease targets. <strong>Results:</strong> Thirty-four effective ingredients and 130 traditional Chinese medicine-disease targets were screened out for the treatment of OA. The GO functions of traditional Chinese medicine-disease targets mainly included cytokine activity, cytokine receptor binding, nuclear receptor activity, transcription factor activity, proximal promoter DNA-binding transcription activator activity, DNA-binding transcription activator activity, phosphatase binding and so on. KEGG pathways involved in traditional Chinese medicine-disease targets mainly included TLR4 signaling pathway, TNF signaling pathway, IL-17 signaling pathway, MAPK signaling pathway, PI3K/AKT signaling pathway, apoptotic signaling pathway and so on. <strong>Conclusion:</strong> Network pharmacology may predict the multiple targets and multiple signaling pathways in Drynariae Rhizoma-Epimedii Folium treatment for OA, providing new ideas for future research.展开更多
Objective:To study the effect of rhizoma drynariae extract on osteoblast viability and proliferation as well as related gene expression. Methods:SD rats were selected, and the osteoblasts in the femur were isolated, c...Objective:To study the effect of rhizoma drynariae extract on osteoblast viability and proliferation as well as related gene expression. Methods:SD rats were selected, and the osteoblasts in the femur were isolated, cultured and divided into the rhizoma drynariae group that were treated with 0.02 g/mL rhizoma drynariae extract and the control group that were treated with serum-free medium;24 h, 36 h and 48 h after treatment, CCK-8 kits were used to determine osteoblast proliferation activity, and fluorescent quantitative PCR kits were used to determine the mRNA expression of osteogenic activity-related genes and proliferation-related genes.Results:24 h, 36 h and 48h after treatment, osteoblast proliferation activity of rhizoma drynariae group was significantly higher than that of control group;Runx2, OPN, OCN, ALP, OPG, PI3K, AKT, EKR1/2, CyclinD1, CDK2, CDK4 and E2F mRNA expression in osteoblasts of rhizoma drynariae group were significantly higher than those of control group. Conclusion:Rhizoma drynariae extract can promote osteoblast proliferation, increase the osteogenetic activity of osteoblasts and also accelerate the cell cycle process through the PI3K/AKT and ERK1/2 signaling pathways.展开更多
文摘[Objectives]To accurately determine the naringin content in Rhizoma Drynariae.[Methods]The high performance liquid chromatography(HPLC)method was applied in the determination of the naringin content in Rhizoma Drynariae.The sample was sonicated at room temperature.The mobile phase was 0.1%phosphoric acid-acetonitrile(75∶25),detected by diode array detector at the wavelength of 284 nm,and quantified by external standard method.[Results]The linearity of naringin was good in the concentration range of 5-500μg/mL with a correlation coefficient of 0.9999.[Conclusions]This method has good linearity,easy operation,correctness and reproducibility as required,and is expected to provide a method for the determination of naringin content in Rhizoma Drynariae.
文摘<strong>Objective:</strong> To investigate the potential mechanism of Drynariae Rhizoma-Epimedii Folium in the treatment of osteoarthritis (OA) based on network pharmacology. <strong>Methods:</strong> The potential active constituents and targets of Drynariae Rhizoma-Epimedii Folium were screened through the traditional Chinese medicine (TCM) systems pharmacology database and analysis platform (TCMSP). Genecards database is used to find relevant targets of OA. The targets of “Drynariae Rhizoma-Epimedii Folium” were mapped to the targets of OA, and used Cytoscape software to build a “drug-ingredient-target-di- sease” regulatory network and protein protein interaction (PPI) network. R software was used to analyze the Gene ontology (GO) function and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment of traditional Chinese medicine-disease targets. <strong>Results:</strong> Thirty-four effective ingredients and 130 traditional Chinese medicine-disease targets were screened out for the treatment of OA. The GO functions of traditional Chinese medicine-disease targets mainly included cytokine activity, cytokine receptor binding, nuclear receptor activity, transcription factor activity, proximal promoter DNA-binding transcription activator activity, DNA-binding transcription activator activity, phosphatase binding and so on. KEGG pathways involved in traditional Chinese medicine-disease targets mainly included TLR4 signaling pathway, TNF signaling pathway, IL-17 signaling pathway, MAPK signaling pathway, PI3K/AKT signaling pathway, apoptotic signaling pathway and so on. <strong>Conclusion:</strong> Network pharmacology may predict the multiple targets and multiple signaling pathways in Drynariae Rhizoma-Epimedii Folium treatment for OA, providing new ideas for future research.
基金Natural Science Foundation of Inner Mongolia No:2013MS0752.
文摘Objective:To study the effect of rhizoma drynariae extract on osteoblast viability and proliferation as well as related gene expression. Methods:SD rats were selected, and the osteoblasts in the femur were isolated, cultured and divided into the rhizoma drynariae group that were treated with 0.02 g/mL rhizoma drynariae extract and the control group that were treated with serum-free medium;24 h, 36 h and 48 h after treatment, CCK-8 kits were used to determine osteoblast proliferation activity, and fluorescent quantitative PCR kits were used to determine the mRNA expression of osteogenic activity-related genes and proliferation-related genes.Results:24 h, 36 h and 48h after treatment, osteoblast proliferation activity of rhizoma drynariae group was significantly higher than that of control group;Runx2, OPN, OCN, ALP, OPG, PI3K, AKT, EKR1/2, CyclinD1, CDK2, CDK4 and E2F mRNA expression in osteoblasts of rhizoma drynariae group were significantly higher than those of control group. Conclusion:Rhizoma drynariae extract can promote osteoblast proliferation, increase the osteogenetic activity of osteoblasts and also accelerate the cell cycle process through the PI3K/AKT and ERK1/2 signaling pathways.
