Effect of lactobacillus on the gut microflora and barrier function of the rats with abdominal infection

AIM:To investigate the effect of probiotics supplemented by gut on the tight junctions of epithelial cells, barrier function and the microflora of rats with abdominal infection. METHODS: After the model of cecal ligation and perforation established, SD rats were divided into two groups: parenteral nutrition (PN) group and PIM+probiotics (probiotics) group, PN solution was supplemented by neck vein and probiotics was delivered via the jejunostomy tube for five days. Vena cava blood and the homogenated tissue of liver, lung and mesenteric lymph nodes were cultured to determine the bacterial translocation rate (BTR). The ultra-structure of epithelial tight junctions and microvilli of the gut were observed by electron microscopy; occluding expression was measured by indirect-immune fluorescence method; anaerobic bacterial growth by anaerobic culture and DNA fingerprint of bacterial colonies of the feces by PCR. RESULTS: The quantity of lactobacteria and bifydobacteria in probiotics group was higher than that of PN group. The profiles of DNA fingerprint expression in probiotics group were similar to that in the normal group, a new 16S rDNA sequence appeared in the profile in PN group. The occludin expression, the integrality of the gut epithelial tight junction and microvilli in probiotics group were improved as compared with PN group. The BTR and endotoxin in blood were reduced more significantly in probiotics group as compared with PN group. CONCLUSION: The probiotics could improve the gut microflora disturbance, increase occludin expression, maintain the gut epithelial tight junction and decrease the bacterial translocations rate.

Impacts of dietary supplementation of bamboo vinegar and charcoal powder on growth performance,intestinal morphology,and gut microflora of large-scale loach Paramisgurnus dabryanus

Although accumulating data demonstrate that dietary supplementation of bamboo vinegar and charcoal powder(BVC)can significantly strengthen the immunity and boost the growth of domestic animals,its application potency still awaits verification in commercial fish species such as the large-scale loach Paramisgurnus dabryanus.The impacts of 90-day dietary supplementation of 1%and 2%BVC on survival and growth performance of the loach,and on the intestinal morphological characteristics and gut microflora were analyzed.Our data show that the large-scale loach supplied with BVC at the experimental doses had significant higher survival rates and better growth performance(indicated by greater weight gain(1.13–1.14 times),higher specific growth rate(1.04 times),and lower feed conversion ratio(0.88–0.89 times))compared to that of the control(P<0.05).Histological examination revealed significant longer villus(3.22–5.54 times),deeper crypt(1.77–1.87 times),and thicker muscle(1.59–3.17 times)in the intestines of large-scale loach fed with BVC(P<0.05).Furthermore,we found that the gut microflora consisted of significantly fewer proportions of potential pathogenic bacterial species(Aeromonas veronii and Escherichia coli),but significantly greater proportions of beneficial microbes(Lactococus raffinolactis and Faecalibacterium prausnitzii).Therefore,dietary intake of BVC can promote intestinal tract development and optimize gut microflora,by which the survival and growth of large-scale loach may be improved.

Study on the interaction between β-carotene and gut microflora using an in vitro fermentation model

β-Carotene,a typical non-oxygenated carotenoid,is the most efficient source of retinol(VA).The low bio-availability ofβ-carotene lead to large accumulation in colon;however,the relationship betweenβ-carotene and gut microflora remains unclear.This study intends to explore the interaction betweenβ-carotene and gut microflora using an in vitro fermentation model.After 24 h fermentation,the degradation rate ofβ-carotene was(64.28±6.23)%,which was 1.46 times that of the group without gut microflora.Meanwhile,the production of VA was nearly 2 times that of the group without gut microflora,indicating that the gut microflora can metabolizeβ-carotene into VA.β-Carotene also influences the production of short-chain fatty acids(SCFAs),the production of total SCFAs in 0.5 mg/mLβ-carotene(BCM)group was(44.00±1.16)mmol/L,which was 2.26 times that of the blank control(BLK)group.Among them,the production of acetic acid in BCM group was(19.06±0.82)mmol/L,which was 2.64 time that of the BLK group.Furthermore,β-carotene significantly affected the structure and composition of gut microflora,increasing the abundance of Roseburia,Parasutterella and Lachnospiraceae,and decreasing the abundance of Dialister,Collinsella and Enterobacter(P<0.05).This study provides a new way to understand howβ-carotene works in human body with gut microflora.

Research progress on adaptive modifications of the gut microflora and regulation of host glucose and lipid metabolism by cold stimulation

The gut microflora is a combination of all microbes in intestine and their microenvironment,and its change can sensitively reflect the relevant response of the body to external environment and remarkably affect body's metabolism as well.Recent studies have found that cold exposure affects the body's gut microflora,which can lead to changes in the body's metabolism of glucose and lipid.This review summarizes recent research on the effects of cold exposure on gut microbes and metabolism of glucose and lipid,aiming to provide some new ideas on the approaches and measures for the prevention and treatment of diabetes and obesity.

Evaluation of the effects of simulated in vitro digestion by Gastrodia elata on biological activity and gut microflora regulation

Gastrodia elata (G. elata ) is a traditional Chinese herbal medicine. In recent years, G. elata has been widely used in food and pharmaceutical industries because of its excellent health benefits. However, the stability and bioactivity of phenolic compounds from G. elata after in vitro gastrointestinal digestion remain unknown. In this study, the aqueous extract of G. elata rhizomes was used to simulate gastrointestinal digestion and colonic fermentation in vitro . This study aimed to evaluate the bioaccessibility during the simulation of gastrointestinal and colonic fermentation and to clarify the effects of different digestion stages on the anti-oxidant and enzyme inhibitory activities and intestinal health of samples in vitro . The results indicated that the total polyphenol and flavonoid contents after colonic fermentation for 72 h were 5.48- and 3.24- fold higher than those without digestion, respectively. Except for gastrodigenin and ferulic acid, the contents and bioaccessibility of the other phenols after fermentation were higher than those in the small-intestine stage. Antioxidant activity was positively correlated with the total polyphenol content. The inhibitory activities of pancrelipase and cholinesterase were enhanced by gastric digestion and colonic fermentation. The sample promoted the production of Lactobacillus and decreased the abundance of pathogenic bacteria. Moreover, the content of short-chain fatty acids also exhibited an upward trend. Therefore, G. elata has the potential to develop into a health food with anti-oxidant, anti-obesity, and intestinal health functions.

Dietary organic acid and fiber sources affect performance,intestinal morphology, immune responses and gut microflora in broilers

This experiment was designed to investigate the effects of a dietary organic acid(OA) mixture and 2 fiber sources on performance, intestinal morphology, immune responses and gut microflora in broilers. A total of 390 one-day-old broiler chicks(Ross 308) were allocated to 6 dietary treatments with 5 replicate pens and 13 chicks each based on a factorial arrangement(2 x 3) in a completely randomized design. The experiment lasted 42 d. The following experimental diets and as well as their interaction were considered: a basal diet supplemented with or without OA(0 or 1 g/kg) and 2 fiber sources(sugar beet pulp[soluble fiber] or rice hull [insoluble fiber];0 or 30 g/kg). Dietary supplementation of OA increased daily weight gains of broilers across the entire rearing period(P < 0.05). The dietary fibrous materials did not affect the performance of broilers. Antibody titer against influenza disease virus was higher in birds fed diets containing rice hull compared with other experimental groups(P < 0.05). The population of Lactobacillus bacteria was greater in birds fed OA-added diets without or with 30 g/kg rice hull supplementation compared with other experimental groups(P < 0.05). In conclusion, dietary supplemental OA improved performance of broilers, and dietary supplemental OA with rice hull enhanced humoral immune responses.

A Comparative Study on Rat Intestinal Epithelial Cells and Resident Gut Bacteria (ii) Effect of Arsenite

Objective In order to use facultative gut bacteria as an alternate to animals for the initial gastrointestinal toxicity screening of heavy metals, a comparative study on rat intestinal epithelial cells and resident gut bacteria was undertaken. Methods in vitro growth rate of four gut bacteria, dehydrogenase （DHA） and esterase （EA） activity test, intestinal epithelial and bacterial cell membrane enzymes and in situ effect of arsenite were analysed. Results Growth profile of mixed resident population of gut bacteria and pure isolates of Escherichia coli, Pseudomonas sp., Lactobacillus sp., and Staphylococcus sp. revealed an arsenite （2-20 ppm） concentration-dependent inhibition. The viability pattern of epithelial cells also showed similar changes. DHA and EA tests revealed significant inhibition （40%-72%） with arsenite exposure of 5 and 10 ppm in isolated gut bacteria and epithelial cells. Decrease in membrane alkaline phosphatase and Ca^2＋-Mg^2＋-ATPase activities was in the range of 33%-55% in four bacteria at the arsenite exposure of 10 ppm, whereas it was 60%-65% in intestinal epithelial villus cells, in situ incubation of arsenite using intestinal loops also showed more or less similar changes in membrane enzymes of resident gut bacterial population and epithelial cells. Conclusion The results indicate that facultative gut bacteria can be used as suitable in vitro model for the preliminary screening of arsenical gastrointestinal cytotoxic effects.

冠突散囊菌发酵液对高脂饮食大鼠肥胖和肠道菌群的影响

本研究旨在探究突散囊菌发酵液对肥胖大鼠脂质代谢和肠道菌群紊乱的影响,为其开发利用提供科学依据。SD大鼠被随机分为正常组、模型组和冠突散囊菌发酵液干预的低、中、高剂量组,以及血脂康阳性对照组。通过高脂喂养建立食源性肥胖大鼠模型,正常组和模型组给予无菌水灌胃,各剂量组灌胃不同剂量冠突散囊菌发酵液。实验为期8周,末次给药后收集粪便和血清,用于肠道菌群分析和相关生化指标测定,并摘取肝脏进行病理学观察。结果显示,模型组大鼠体质量和Lee′s指数显著增加,血脂发生异常,肠道菌群多样性显著降低。冠突散囊菌发酵液显著降低了肥胖大鼠血清中总胆固醇、甘油三酯和低密度脂蛋白胆固醇的含量,提高了高密度脂蛋白胆固醇的含量。测序结果亦显示,冠突散囊菌发酵液显著提高大鼠肠道菌群的多样性,上调瘤胃球菌NK4A214_group、UCG-005、Christensenellaceae_R-7_group等菌群的丰度,下调拟杆菌属Bacteroides和Turicibacter的丰度。LefSe分析还显示,冠突散囊菌发酵液可显著增加大鼠肠道中乳酸菌Lactobacillus丰度,提高肠道的防御功能。综上所述,冠突散囊菌发酵液能够有效改善高脂饮食肥胖大鼠的脂代谢及肠道菌群紊乱。

纳米塑料和酮康唑对霍甫水丝蚓肠道的影响

采用亚急性毒性试验方法,研究原始及不同官能团修饰的聚苯乙烯纳米塑料(NPs)和环境相关浓度酮康唑(KCZ)共暴露条件下霍甫水丝蚓的生物累积、组织病理学及肠道微生物响应.结果表明,共暴露7d,3种NPs均能被水丝蚓吸收并累积在体内,氨基修饰NPs的累积水平最低(2.78ng/mg),羧基修饰NPs的累积水平最高(22.85ng/mg).共存NPs显著减少了KCZ的生物累积,抑制率在第7d达到最高(88.9%~94.8%).KCZ单独及与NPs共暴露诱导了霍甫水丝蚓的肠道损伤,出现表皮和肠细胞退化、表皮和肠细胞增生、表皮表面不规则和产氯组织变性等病理变化.3种NPs的共存都显著降低了KCZ对胃蛋白酶和纤维素酶活性的抑制;而氨基修饰NPs的共存使D乳酸盐含量显著下降,羧基修饰NPs的共存使二胺氧化酶活性显著下降.KCZ单独暴露显著增加了水丝蚓肠道微生物的多样性,官能团修饰NPs的共存使微生物多样性降低,并改变了门水平的丰度和优势菌属,尤其是氨基修饰NPs共存时优势菌属均为致病菌,增加了水丝蚓感染的风险.

饲粮中添加油莎豆或油莎豆粕对妊娠后期母猪繁殖性能、血清免疫指标和粪便微生物组成的影响

本试验旨在研究饲粮中添加油莎豆或油莎豆粕对妊娠后期母猪繁殖性能、血清免疫指标、胎盘活性因子、粪便短链脂肪酸含量和粪便微生物组成的影响。本试验选择胎次、体况和妊娠期一致的初产妊娠母猪70头,随机分为7组,分别为对照组(CK组)、5%油莎豆添加组(5%TN组)、10%油莎豆添加组(10%TN组)、15%油莎豆添加组(15%TN组)、10%油莎豆粕添加组(10%TNM组)、15%油莎豆粕添加组(15%TNM组)、20%油莎豆粕添加组(20%TNM组),每组10个重复,每个重复1头猪。试验从母猪妊娠第70天开始至分娩结束。结果表明:1)与对照组相比,5%TN组和20%TNM组的产程显著缩短(P<0.05);10%TN组和10%TNM组的健仔率显著提高(P<0.05)。2)与对照组相比,10%TN组、15%TN组和10%TNM组血清白细胞介素-6(IL-6)含量显著降低(P<0.05);油莎豆添加组、10%TNM组和15%TNM组血清白细胞介素-10(IL-10)含量显著升高(P<0.05);油莎豆添加组、油莎豆粕添加组血清肿瘤坏死因子-α(TNF-α)和内毒素含量均显著降低(P<0.05);油莎豆添加组血清连蛋白含量显著降低(P<0.05);油莎豆添加组胎盘组织中一氧化氮(NO)、血管内皮生成因子(VEGF)含量和内皮型一氧化氮合酶(eNOS)活性显著提升(P<0.05)。3)与对照组相比,油莎豆添加组、油莎豆粕添加组粪便中的丙酸含量显著升高(P<0.05);10%TN组和10%TNM组粪便中的丁酸含量显著升高(P<0.05);10%TNM组和20%TNM组粪便中的异丁酸和异戊酸含量显著升高(P<0.05)。4)在属水平粪便微生物组成上,与对照组相比,10%TN组毛螺菌科NK4A136群、狄氏副拟杆菌属、巨球型菌属、g_Lachnoclostridium、普雷沃氏菌科UCG-004群相对丰度显著升高(P<0.05);10%TNM组g_Lachnoclostridium、普雷沃氏菌科UCG-004群相对丰度显著升高(P<0.05)。综上所述,饲粮中添加10%油莎豆或10%油莎豆粕可以显著提升妊娠后期母猪的健仔率和血清免疫指标;饲粮中添加油莎豆可以显著提升母猪胎盘活性因子的含量或活性;饲粮中添加10%油莎豆或10%油莎豆粕可以调控母猪肠道微生物组成,增加肠道中短链脂肪酸含量。

推拿不同部位对孤独症谱系障碍模型鼠行为学及肠道菌群的影响

目的探究不同部位的推拿对丙戊酸(VPA)诱导的孤独症谱系障碍(ASD)模型鼠行为学和肠道菌群的影响。方法将20只怀孕12.5天的Sprague Dawley(SD)雌性大鼠随机分为VPA模型组[腹腔注射VPA(600 mg/kg),n=15]和生理盐水组(腹腔注射等量生理盐水,n=5)。将注射生理盐水的子代雄鼠作为对照组,将注射VPA的子代雄鼠随机分为VPA组、背部组和腹部组(n=7)。在出生后第21天,通过三箱社交、旷场实验和埋珠实验观察四组大鼠的社交能力、焦虑行为和刻板行为,并开始对背部组和腹部组进行推拿,每天2次,持续14天。第35天,再次进行行为学检测,并取肠道内容物进行物种组成与结构分析、标志物种与差异物种分析。结果(1)行为学结果显示,与VPA组相比,背部组大鼠社交时间和旷场中心区运动时间显著增加(P<0.05),埋珠数量明显减少(P<0.01),说明其社交、焦虑和刻板行为得到改善;与VPA组相比,腹部组大鼠埋珠数量显著减少(P<0.05),刻板行为改善;埋珠实验中,背部组较腹部组埋珠数量更少,刻板行为改善更显著(P<0.05),而三箱社交和旷场实验两组结果无显著差异(P>0.05)。(2)肠道微生物检测结果显示,在多样性分析中,与VPA组相比,背部组和腹部组肠道菌群丰富度显著增加(P<0.05),多样性增加(背部组P<0.05,腹部组P<0.01);在差异性分析中,在门水平上,与VPA组相比,腹部组厚壁菌门相对丰度呈现较为明显的降低趋势(P<0.05);在属水平上,与VPA组相比,背部组和腹部组的乳杆菌属相对丰度较VPA组显著下降(P<0.05);标志物种分析中,与VPA组相比,背部组布劳特氏菌属显著增加(P<0.05)。结论推拿能对ASD模型大鼠行为学和肠道菌群结构产生影响;推拿背部对ASD模型鼠的社交能力、焦虑行为和刻板行为疗效显著,推拿腹部仅对刻板行为有明显效果;推拿背部与推拿腹部均使ASD模型鼠肠道菌群丰富度和多样性增加,其中推拿腹部对肠道微生物多样性的改善更显著,并使物种分布更均匀。

阻塞性睡眠呼吸暂停低通气综合征与肠道菌群关系的研究进展

阻塞性睡眠呼吸暂停低通气综合征(OSAHS)是一种在睡眠时发生间歇性缺氧和复氧损伤的呼吸疾病。OSAHS与肠道菌群密切相关,可诱发肠道菌群紊乱、肠道代谢产物改变、肠道屏障受损和全身炎症反应;反过来,肠道菌群紊乱又可促进OSAHS进展,然而相关机制尚未完全阐明。本文就OSAHS与肠道菌群的相关研究进展进行综述。

基于脑肠轴探讨肠道菌群调控腹泻型肠易激综合征的研究进展

腹泻型肠易激综合征是以内脏高敏感性为主要发病机制的一种肠道功能紊乱性疾病,主要临床表现为精神刺激引发的腹痛腹泻,与中医学中肝郁脾虚型泄泻症状高度相似。近期有研究指出,在腹泻型肠易激综合征发病机制中,脑肠轴紊乱导致的内脏高敏感性是关键,肠道菌群失调亦会引起和加剧内脏高敏感性。文章就中枢和外周调控内脏高敏性的角度分析了肠道菌群对脑肠轴5-HT的调控作用,进一步丰富腹泻型肠易激综合征的中医药治疗理论,为临床诊疗提供新思路。

人体肠道微生物多样性和功能研究进展

人体肠道中庞大而复杂的微生物群落对人体自身代谢表型有深远的影响。肠道微生物群落在亚种或菌株水平上表现出极大的多样性。利用微生物分子生态学、元基因组学和代谢组学研究方法,发现肠道微生物与宿主表现出共进化的特点,肠道微生物群落及其基因组为宿主提供了互补的遗传和代谢功能,表现出互惠共生关系。但是,肠道微生物群落中影响宿主代谢表型的关键功能菌鉴定及其作用模式问题仍然悬而未决,综合运用多种高通量研究方法和多维数据分析方法可能成为解决这个问题的突破口。

松花粉对慢性应激大鼠肠道微生态的影响

目的观察慢性应激对大鼠肠道微生态的影响及松花粉的保健作用。方法采用大鼠慢性应激动物模型,3种刺激因素噪声、强迫游泳和头低位束缚随机作用于大鼠,实验期7周左右。用选择性培养基平皿计数法检测大鼠粪便菌群中几种代表性菌种的数量;用酶联免疫法测定大鼠血清中应激激素的含量。结果慢性应激实验对照组大鼠,粪便中以益生菌双歧杆菌为主的原籍菌数量减少,而过路菌肠杆菌和肠球菌相应增多,即发生了肠道微生态紊乱的现象;进食含5%松花粉饲料的实验组大鼠双歧杆菌数量减少不明显。结论在慢性应激条件下,大鼠发生了以原籍菌双歧杆菌减少为特征的肠道微生态紊乱的现象,食用松花粉能够有效减轻慢性应激因素导致的大鼠肠道微生态紊乱。

草鱼和银鲫肠道产消化酶细菌的研究

检测了分别从草鱼(Ctenopharyngodon idellus)和银鲫(Carassius auratus gibelio)肠道中分离的180株细菌的蛋白酶、脂肪酶、淀粉酶和纤维素酶的产酶能力。结果显示,两种鱼肠道内可分泌胞外消化酶的细菌包括Aero-monas(气单胞菌属,Aer.)、Vibrio(弧菌属,Vib.)、Bacillus(芽孢杆菌属,Bac.)、Pseudomonas(假单胞菌属,Pse.)四个种属的细菌,Aer.在其中占主要优势,45.71%的Aer.可分泌胞外消化酶。草鱼可分泌上述四种胞外消化酶的菌株共有33株,占肠道菌总数的36.67%;银鲫43株,占47.78%。产酶菌的分布上,草鱼中肠内产消化酶细菌数量显著多于前肠和后肠(P<0.05),前、中、后肠分别是6株、20株和7株;银鲫中肠和后肠数量差异不显著,前肠分布最少。草鱼分泌蛋白酶、脂肪酶、淀粉酶和纤维素酶的菌株分别有21株(23.33%)、10株(11.11%)、30株(33.33%)和16株(17.78%)。银鲫肠道内未检测到可分泌纤维素酶的细菌,蛋白酶、脂肪酶和淀粉酶菌株的数量分别是21株、37株和17株。可见鱼类肠道细菌对食饵消化有重要作用。

乳铁蛋白素B和天蚕素P1对投喂大肠杆菌断奶仔猪生长及肠道微生物区系的影响

本研究旨在探讨投喂大肠杆菌后乳铁蛋白素B(Lfcin B)和天蚕素P1(Cec P1)对断奶仔猪生长及肠道微生物区系影响。选用48头28日龄断奶的德国大白杂交二代断奶仔猪[(7.18±1.32)kg],根据体重、窝别、性别将其随机分成4组:对照组、产肠毒素组(ETEC)、乳铁蛋白素组(Lfcin B)、天蚕素组(Cec P1),每组2个重复,每个重复6头猪,试验期为12 d。结果表明,1)试验期间对照组、ETEC组、Lfcin B组、Cec P1组仔猪体重及平均日增重均差异不显著(P>0.05)。2)ETEC组仔猪腹泻率显著高于其他组(P<0.05),而其他组之间差异不显著(P>0.05)。在进行第1次攻毒后,即仔猪29日龄,ETEC组、Lfcin B组和Cec P1组仔猪的粪便干物质均显著下降(P<0.05),第2次攻毒后的第2天,即仔猪31日龄,Lfcin B组和Cec P1组的仔猪分别饲喂了Lfcin B和Cec P1,对照组仔猪的粪便干物质仍显著高于ETEC组和LfcinB组(P<0.05),但已与Cec P1组差异不显著(P>0.05)。3)各组仔猪的肠道微生物区系差异不显著(P>0.05)。由此可知,各组仔猪生长及肠道微生物区系差异不显著,但Cec P1来源于猪肠道微生物寄生线虫,比Lfcin B更能适应肠道环境,更有利于仔猪健康。

茶多酚体内吸收、分布、代谢和排泄研究进展

茶多酚是茶叶中重要的活性物质,具有多种保健功效,例如预防代谢综合症、癌症以及神经退行性疾病等。茶多酚体内的吸收、分布、代谢和排泄(ADME)与茶多酚的健康功效紧密相关。本文综述了近年来荼多酚体内ADME的研究结果,表明EGCG在人体内的相对生物利用度并不低于小鼠,灌胃给药后肠道中的EGCE含量最高,而在脑组织中的分布极少;同时可以发现肝脏是体内EGCG的主要处置器官,之后通过胆汁排泄。对于2种儿荼素而言,非酯型儿茶素(EC和EGC)口服后在体内相对生物利用度高于酯型儿茶素(EGCG和ECG)。系统地比较了荼多酚在人体及其他动物模型中的代谢规律,为进一步研究和开发茶多酚提供参考。

谷物膳食纤维——戊聚糖与肠道菌群调节研究进展

流行病学研究表明,谷物膳食纤维对慢性代谢性疾病,如肥胖、Ⅱ型糖尿病、心脑血管疾病以及结肠癌等具有预防作用。戊聚糖又称为阿拉伯木聚糖,是谷物膳食纤维的主要组成部分。戊聚糖在结肠部位被特定的具有戊聚糖水解酶的细菌所降解,能够增殖肠道中的双歧杆菌和乳杆菌等有益菌。不同程度的聚合度、取代度、交联度以及侧链的分布均会影响戊聚糖的益生特性。戊聚糖所具有的生理功效不仅取决于多糖分子,还源于与其相连的阿魏酸在肠道中缓慢而持续的释放和吸收,并发挥其抗氧化功能。

肠道微生物菌群生物转化天然多酚类化合物研究进展

多酚经人体摄入后,由肠道微生物产生的酶分解为更易被人体吸收的酚酸等小分子物质,增加了多酚的生物利用度和活性。这一特性在促进人体健康和食品加工方面有一定的应用潜力。本文综述多酚化合物的分类、多酚在食品中的分布和吸收利用、肠道微生物对多酚的生物转化产物及形成机制等问题,以期为开发益于人体健康的高质量食品和功能性食品提供依据。