Dietary fiber, resistant to host-mediated digestion in the small intestine due to lack of endogenous enzymes, impacts many facets of animal health and is associated with gut development especially in young monogastric...Dietary fiber, resistant to host-mediated digestion in the small intestine due to lack of endogenous enzymes, impacts many facets of animal health and is associated with gut development especially in young monogastrics. Furthermore, it can be used as in-feed antibiotic alternative. Chicory （Cichorium intybus L.） forage with high content of pectin （uronic acids as building blocks） is a novel class of dietary fiber that is chemically different from cereal grains （with high content of arabinoxylans）. In the present study, we investigated effects of dietary inclusion of chicory forage on digestibility, gut morphology and microbiota in broilers and young pigs. In the chicken experiment, 160 1-d old broiler chicks were fed 3 nutritionally balanced diets for 30 d including a cereal-based diet and 2 diets with part of the cereals substituted with 60 and 120 g/k9 chicory forage （CF60 and CF120], whereas in the pig experiment, 18 seven-wk old Yorkshire pigs were fed 3 diets for 18 d including a cereal-based diet and 2 diets with 80 and 160 g/kg chicory forage inclusion （CF80 and CF160）. Our results showed that young pigs were capable to utilize chicory forage well with higher total tract apparent digestibility （TTAD） of all fiber fractions, particularly uronic acid, compared with the control （P 〈 0.01）. In contrast, a decreased TTAD of all fiber fractions was observed in chickens fed on diet CF120 （P 〈 0.05）. Moreover, diet induced changes in gut morphology were observed in the large intestine of chickens. The alteration of cecal mucosal thickness was further positively correlated with TTAD of non-starch polysaccharides （NSP） and its constituent sugars （P 〈 0.05）. In addition, in pigs, terminal restriction fragment length polymorphism （T-RFLP） analysis of intestinal microbiota revealed substantial dietary effects （cereal control diet vs. chicory forage inclusion） on the relative abundance of 2 dominant bacterial phylotypes （Prevotella sp. vs. Roseburia sp.） respectively （P 〈 0.05）. In conclusion, our data showed that chicory forage （Cichorium intybus L.）, a novel dietary fiber source in animal nutrition, have potential beneficial properties as fiber ingredient in diets for both pigs and chickens.展开更多
A total of 294 one-day-old Cobb broiler chickens were used to investigate the effects of four Lactobacillus strains on gut microbial profile and production performance. The six dietary treatments, each with 7 replicat...A total of 294 one-day-old Cobb broiler chickens were used to investigate the effects of four Lactobacillus strains on gut microbial profile and production performance. The six dietary treatments, each with 7 replicates were: 1) basal diet(negative control), 2) one of four strains of Lactobacillus(tentatively identified as Lactobacillus johnsonii, Lactobacillus crispatus, Lactobacillus salivarius and an unidentified Lactobacillus sp.) and 3) basal diet with added zinc-bacitracin(ZnB, 50 mg/kg). Results showed that the addition of probiotic Lactobacillus spp. to the feed did not significantly improve weight gain, feed intake and feed conversion rate(FCR) of broiler chickens raised in cages during the 6-week experimental period,but tended to increase the number of total anaerobic bacteria in the ileum and caeca, and the number of lactic acid bacteria and lactobacilli in the caeca; and to significantly increase the small intestinal weight(jejunum and ileum). Furthermore, all 4 probiotics tended to reduce the number of Enterobacteria in the ileum, compared with the control treatments. The probiotics did not affect the p H and the concentrations of short chain fatty acids(SCFA) and lactic acid in both the ileum and caeca.展开更多
At birth the piglet＇s immune system is immature and it is dependent upon passive maternal protection until weaning.The piglet＇s mucosal immune system develops over the first few weeks but has not reached maturity at...At birth the piglet＇s immune system is immature and it is dependent upon passive maternal protection until weaning.The piglet＇s mucosal immune system develops over the first few weeks but has not reached maturity at weaning ages which are common on commercial farms. At weaning piglets are presented with a vast and diverse range of microbial and dietary/environmental antigens. Their ability to distinguish between antigens and mount a protective response to potential pathogens and to develop tolerance to dietary antigens is critical to their survival and failure to do so is reflected in the high incidence of morbidity and mortality in the post-weaning period. A growing recognition that the widespread use of antibiotics to control infection during this critical period should be controlled has led to detailed studies of those factors which drive the development of the mucosal immune system, the role of gut microbiota in driving this process, the origin of the bacteria that colonise the young piglet＇s intestine and the impact of rearing environment. This review briefly describes how the mucosal immune system is equipped to respond ＂appropriately＂ to antigenic challenge and the programmed sequence by which it develops. The results of studies on the critical interplay between the host immune system and gut microbiota are discussed along with the effects of rearing environment. By comparing these with results from human studies on the development of allergies in children, an approach to promote an earlier maturation of the piglet immune system to resist the challenges of weaning are outlined.展开更多
Colonization and development of the gut microbiome is a crucial consideration for optimizing the health and performance of livestock animals. This is mainly attributed to the fact that dietary and management practices...Colonization and development of the gut microbiome is a crucial consideration for optimizing the health and performance of livestock animals. This is mainly attributed to the fact that dietary and management practices greatly influence the gut microbiota, subsequently leading to changes in nutrient utilization and immune response. A favorable microbiome can be implanted through dietary or management in-terventions of livestock animals, especially during early life. In this review, we explore all the possible factors (for example gestation, colostrum, and milk feeding, drinking water, starter feed, inoculation from healthy animals, prebiotics/probiotics, weaning time, essential oil and transgenesis), which can influence rumen microbiome colonization and development. We discuss the advantages and disadvantages of potential strategies used to manipulate gut development and microbial colonization to improve the production and health of newborn calves at an early age when they are most susceptible to enteric disease. Moreover, we provide insights into possible interventions and their potential effects on rumen development and microbiota establishment. Prospects of latest techniques like transgenesis and host genetics have also been discussed regarding their potential role in modulation of rumen microbiome and subsequent effects on gut development and performance in neonatal ruminants.展开更多
基金supported by Formas(Swedish Research Council for Environment,Agricultural Sciences and Spatial Planning)funding from the Swedish Foundation Cerealia FoU
文摘Dietary fiber, resistant to host-mediated digestion in the small intestine due to lack of endogenous enzymes, impacts many facets of animal health and is associated with gut development especially in young monogastrics. Furthermore, it can be used as in-feed antibiotic alternative. Chicory （Cichorium intybus L.） forage with high content of pectin （uronic acids as building blocks） is a novel class of dietary fiber that is chemically different from cereal grains （with high content of arabinoxylans）. In the present study, we investigated effects of dietary inclusion of chicory forage on digestibility, gut morphology and microbiota in broilers and young pigs. In the chicken experiment, 160 1-d old broiler chicks were fed 3 nutritionally balanced diets for 30 d including a cereal-based diet and 2 diets with part of the cereals substituted with 60 and 120 g/k9 chicory forage （CF60 and CF120], whereas in the pig experiment, 18 seven-wk old Yorkshire pigs were fed 3 diets for 18 d including a cereal-based diet and 2 diets with 80 and 160 g/kg chicory forage inclusion （CF80 and CF160）. Our results showed that young pigs were capable to utilize chicory forage well with higher total tract apparent digestibility （TTAD） of all fiber fractions, particularly uronic acid, compared with the control （P 〈 0.01）. In contrast, a decreased TTAD of all fiber fractions was observed in chickens fed on diet CF120 （P 〈 0.05）. Moreover, diet induced changes in gut morphology were observed in the large intestine of chickens. The alteration of cecal mucosal thickness was further positively correlated with TTAD of non-starch polysaccharides （NSP） and its constituent sugars （P 〈 0.05）. In addition, in pigs, terminal restriction fragment length polymorphism （T-RFLP） analysis of intestinal microbiota revealed substantial dietary effects （cereal control diet vs. chicory forage inclusion） on the relative abundance of 2 dominant bacterial phylotypes （Prevotella sp. vs. Roseburia sp.） respectively （P 〈 0.05）. In conclusion, our data showed that chicory forage （Cichorium intybus L.）, a novel dietary fiber source in animal nutrition, have potential beneficial properties as fiber ingredient in diets for both pigs and chickens.
文摘A total of 294 one-day-old Cobb broiler chickens were used to investigate the effects of four Lactobacillus strains on gut microbial profile and production performance. The six dietary treatments, each with 7 replicates were: 1) basal diet(negative control), 2) one of four strains of Lactobacillus(tentatively identified as Lactobacillus johnsonii, Lactobacillus crispatus, Lactobacillus salivarius and an unidentified Lactobacillus sp.) and 3) basal diet with added zinc-bacitracin(ZnB, 50 mg/kg). Results showed that the addition of probiotic Lactobacillus spp. to the feed did not significantly improve weight gain, feed intake and feed conversion rate(FCR) of broiler chickens raised in cages during the 6-week experimental period,but tended to increase the number of total anaerobic bacteria in the ileum and caeca, and the number of lactic acid bacteria and lactobacilli in the caeca; and to significantly increase the small intestinal weight(jejunum and ileum). Furthermore, all 4 probiotics tended to reduce the number of Enterobacteria in the ileum, compared with the control treatments. The probiotics did not affect the p H and the concentrations of short chain fatty acids(SCFA) and lactic acid in both the ileum and caeca.
文摘At birth the piglet＇s immune system is immature and it is dependent upon passive maternal protection until weaning.The piglet＇s mucosal immune system develops over the first few weeks but has not reached maturity at weaning ages which are common on commercial farms. At weaning piglets are presented with a vast and diverse range of microbial and dietary/environmental antigens. Their ability to distinguish between antigens and mount a protective response to potential pathogens and to develop tolerance to dietary antigens is critical to their survival and failure to do so is reflected in the high incidence of morbidity and mortality in the post-weaning period. A growing recognition that the widespread use of antibiotics to control infection during this critical period should be controlled has led to detailed studies of those factors which drive the development of the mucosal immune system, the role of gut microbiota in driving this process, the origin of the bacteria that colonise the young piglet＇s intestine and the impact of rearing environment. This review briefly describes how the mucosal immune system is equipped to respond ＂appropriately＂ to antigenic challenge and the programmed sequence by which it develops. The results of studies on the critical interplay between the host immune system and gut microbiota are discussed along with the effects of rearing environment. By comparing these with results from human studies on the development of allergies in children, an approach to promote an earlier maturation of the piglet immune system to resist the challenges of weaning are outlined.
基金supported by the Southwest Medical University(SWMU)grant No.42-00040149 that was awarded to Dr.Ahmad Ud Din
文摘Colonization and development of the gut microbiome is a crucial consideration for optimizing the health and performance of livestock animals. This is mainly attributed to the fact that dietary and management practices greatly influence the gut microbiota, subsequently leading to changes in nutrient utilization and immune response. A favorable microbiome can be implanted through dietary or management in-terventions of livestock animals, especially during early life. In this review, we explore all the possible factors (for example gestation, colostrum, and milk feeding, drinking water, starter feed, inoculation from healthy animals, prebiotics/probiotics, weaning time, essential oil and transgenesis), which can influence rumen microbiome colonization and development. We discuss the advantages and disadvantages of potential strategies used to manipulate gut development and microbial colonization to improve the production and health of newborn calves at an early age when they are most susceptible to enteric disease. Moreover, we provide insights into possible interventions and their potential effects on rumen development and microbiota establishment. Prospects of latest techniques like transgenesis and host genetics have also been discussed regarding their potential role in modulation of rumen microbiome and subsequent effects on gut development and performance in neonatal ruminants.
