[Objectives]To study the effect and mechanism of Gynura divaricate(L.)DC(GD)on non-alcoholic fatty liver disease(NAFLD).[Methods]Male mice were randomly divided into 2 groups:normal group and model group.The mice were...[Objectives]To study the effect and mechanism of Gynura divaricate(L.)DC(GD)on non-alcoholic fatty liver disease(NAFLD).[Methods]Male mice were randomly divided into 2 groups:normal group and model group.The mice were fed with high-fat diet(HFD)for 4 weeks to induce NAFLD in the model group.The successfully modeled mice were divided into model group,positive drug group,GD high dose group,and GD low dose group.After 4 weeks of administration,the liver index,serum AST,ALT and blood lipid levels,liver tissue pathological changes,antioxidant enzymes,non-enzymatic antioxidants and inflammatory factors levels were measured in each group,and the expression of NF-κB,Nrf-2 and HO-1 in liver tissues were compared.[Results]GD significantly reduced the serum AST,ALT and blood lipid levels,increased enzyme antioxidant and non-enzymatic antioxidant content,reduced the steatosis and inflammatory infiltration of liver cells,down-regulated the level of inflammatory factors,and inhibited the expression of NF-κB,Nrf-2 and HO-1 in liver tissue.[Conclusions]GD has a protective effect on NAFLD in mice and its mechanism may be related to the regulation of NF-κB and Nrf-2/HO-1 pathways.展开更多
文摘[Objectives]To study the effect and mechanism of Gynura divaricate(L.)DC(GD)on non-alcoholic fatty liver disease(NAFLD).[Methods]Male mice were randomly divided into 2 groups:normal group and model group.The mice were fed with high-fat diet(HFD)for 4 weeks to induce NAFLD in the model group.The successfully modeled mice were divided into model group,positive drug group,GD high dose group,and GD low dose group.After 4 weeks of administration,the liver index,serum AST,ALT and blood lipid levels,liver tissue pathological changes,antioxidant enzymes,non-enzymatic antioxidants and inflammatory factors levels were measured in each group,and the expression of NF-κB,Nrf-2 and HO-1 in liver tissues were compared.[Results]GD significantly reduced the serum AST,ALT and blood lipid levels,increased enzyme antioxidant and non-enzymatic antioxidant content,reduced the steatosis and inflammatory infiltration of liver cells,down-regulated the level of inflammatory factors,and inhibited the expression of NF-κB,Nrf-2 and HO-1 in liver tissue.[Conclusions]GD has a protective effect on NAFLD in mice and its mechanism may be related to the regulation of NF-κB and Nrf-2/HO-1 pathways.
