[Objectives]To study the effect and mechanism of Gynura divaricate(L.)DC(GD)on non-alcoholic fatty liver disease(NAFLD).[Methods]Male mice were randomly divided into 2 groups:normal group and model group.The mice were...[Objectives]To study the effect and mechanism of Gynura divaricate(L.)DC(GD)on non-alcoholic fatty liver disease(NAFLD).[Methods]Male mice were randomly divided into 2 groups:normal group and model group.The mice were fed with high-fat diet(HFD)for 4 weeks to induce NAFLD in the model group.The successfully modeled mice were divided into model group,positive drug group,GD high dose group,and GD low dose group.After 4 weeks of administration,the liver index,serum AST,ALT and blood lipid levels,liver tissue pathological changes,antioxidant enzymes,non-enzymatic antioxidants and inflammatory factors levels were measured in each group,and the expression of NF-κB,Nrf-2 and HO-1 in liver tissues were compared.[Results]GD significantly reduced the serum AST,ALT and blood lipid levels,increased enzyme antioxidant and non-enzymatic antioxidant content,reduced the steatosis and inflammatory infiltration of liver cells,down-regulated the level of inflammatory factors,and inhibited the expression of NF-κB,Nrf-2 and HO-1 in liver tissue.[Conclusions]GD has a protective effect on NAFLD in mice and its mechanism may be related to the regulation of NF-κB and Nrf-2/HO-1 pathways.展开更多
The pyrrolizidine alkaloids(PAs) in leaf of Gynura divaricata(L.) DC.was analyzed by LC-MSn technique.The result shows that two hepatotoxic pyrrolizidine alkaloids(HPAs) are detected from the methanol extract of G.div...The pyrrolizidine alkaloids(PAs) in leaf of Gynura divaricata(L.) DC.was analyzed by LC-MSn technique.The result shows that two hepatotoxic pyrrolizidine alkaloids(HPAs) are detected from the methanol extract of G.divaricata leaf,with relative molecular weight of 335 and 365,respectively and lower content.The constituent with relative molecular weight 335 is a retronecine-type alkaloid,and identified as integerrimine;another constituent with relative molecular weight 365 is an otonecine-type alkaloid,speculated to be isomer of usaramine or 18-hydroxyinterrimine.展开更多
AIM: To investigate the chemical constituents of aerial parts of Gynura divaricata(L.)DC.METHODS: Chemical constituents were isolated with multi-chromatographic methods.Their structures were determined by spectral ana...AIM: To investigate the chemical constituents of aerial parts of Gynura divaricata(L.)DC.METHODS: Chemical constituents were isolated with multi-chromatographic methods.Their structures were determined by spectral analysis and chemical evidence.RESULTS: 10 compounds were obtained and identified as quercetin(Ⅰ),3-O-β-Dglucopyranosyl quercetin(Ⅱ),3-O-β-D-(6→1) glucopyranosyl-αL-rhamnosyl quercetin(Ⅲ),3-O-β-D-(6→1)glucopyranosyl-α-L-rhamnosyl kaemferol(Ⅳ),epi-friedelinol(Ⅴ),epi-friedelinol acetatd(Ⅵ),β-sitosterol(Ⅶ),stigmasterol(Ⅷ),adenosine(Ⅸ) and uridine(Ⅹ).CONCLUSION: All these compounds were isolated from this plant for the first time.展开更多
The dynamic change of total flavonoids content in above-ground part of Gynura bicolor (Willd. ) DC. and G. divaricata (L. ) DC. from two locations was researched. The result shows that the total flavonoids content in ...The dynamic change of total flavonoids content in above-ground part of Gynura bicolor (Willd. ) DC. and G. divaricata (L. ) DC. from two locations was researched. The result shows that the total flavonoids content in G. bicolor and G. divaricata leaf is lower in June and July,higher in the beginning and the middle of August and then has a certain decrease with a great fluctuation. Total flavonoids content in leaf of G. bicolor reaches the highest with a value of 12.031 mg·g-1 at the 15th August,and that in leaf of G. divaricata-1(from Zhejiang of China) and G. divaricata-2 (from South America) is the highest at the 1st August with a value of 17.381 and 19.935 mg·g-1,respectively. The total flavonoids content in leaf of G. bicolor and G. divaricata is higher than that in stem. Except leaf of G. divaricata-2,the total flavonoids content in stem and leaf appears decreasing trend from upper to lower part gradually. Therefore,taking total flavonoids content for an index,it is suitable to harvest upper leaf of G. bicolor,upper leaf and stem of G. divaricata-1,and whole leaf of G. divaricata-2 in August.展开更多
文摘[Objectives]To study the effect and mechanism of Gynura divaricate(L.)DC(GD)on non-alcoholic fatty liver disease(NAFLD).[Methods]Male mice were randomly divided into 2 groups:normal group and model group.The mice were fed with high-fat diet(HFD)for 4 weeks to induce NAFLD in the model group.The successfully modeled mice were divided into model group,positive drug group,GD high dose group,and GD low dose group.After 4 weeks of administration,the liver index,serum AST,ALT and blood lipid levels,liver tissue pathological changes,antioxidant enzymes,non-enzymatic antioxidants and inflammatory factors levels were measured in each group,and the expression of NF-κB,Nrf-2 and HO-1 in liver tissues were compared.[Results]GD significantly reduced the serum AST,ALT and blood lipid levels,increased enzyme antioxidant and non-enzymatic antioxidant content,reduced the steatosis and inflammatory infiltration of liver cells,down-regulated the level of inflammatory factors,and inhibited the expression of NF-κB,Nrf-2 and HO-1 in liver tissue.[Conclusions]GD has a protective effect on NAFLD in mice and its mechanism may be related to the regulation of NF-κB and Nrf-2/HO-1 pathways.
文摘The pyrrolizidine alkaloids(PAs) in leaf of Gynura divaricata(L.) DC.was analyzed by LC-MSn technique.The result shows that two hepatotoxic pyrrolizidine alkaloids(HPAs) are detected from the methanol extract of G.divaricata leaf,with relative molecular weight of 335 and 365,respectively and lower content.The constituent with relative molecular weight 335 is a retronecine-type alkaloid,and identified as integerrimine;another constituent with relative molecular weight 365 is an otonecine-type alkaloid,speculated to be isomer of usaramine or 18-hydroxyinterrimine.
文摘AIM: To investigate the chemical constituents of aerial parts of Gynura divaricata(L.)DC.METHODS: Chemical constituents were isolated with multi-chromatographic methods.Their structures were determined by spectral analysis and chemical evidence.RESULTS: 10 compounds were obtained and identified as quercetin(Ⅰ),3-O-β-Dglucopyranosyl quercetin(Ⅱ),3-O-β-D-(6→1) glucopyranosyl-αL-rhamnosyl quercetin(Ⅲ),3-O-β-D-(6→1)glucopyranosyl-α-L-rhamnosyl kaemferol(Ⅳ),epi-friedelinol(Ⅴ),epi-friedelinol acetatd(Ⅵ),β-sitosterol(Ⅶ),stigmasterol(Ⅷ),adenosine(Ⅸ) and uridine(Ⅹ).CONCLUSION: All these compounds were isolated from this plant for the first time.
文摘The dynamic change of total flavonoids content in above-ground part of Gynura bicolor (Willd. ) DC. and G. divaricata (L. ) DC. from two locations was researched. The result shows that the total flavonoids content in G. bicolor and G. divaricata leaf is lower in June and July,higher in the beginning and the middle of August and then has a certain decrease with a great fluctuation. Total flavonoids content in leaf of G. bicolor reaches the highest with a value of 12.031 mg·g-1 at the 15th August,and that in leaf of G. divaricata-1(from Zhejiang of China) and G. divaricata-2 (from South America) is the highest at the 1st August with a value of 17.381 and 19.935 mg·g-1,respectively. The total flavonoids content in leaf of G. bicolor and G. divaricata is higher than that in stem. Except leaf of G. divaricata-2,the total flavonoids content in stem and leaf appears decreasing trend from upper to lower part gradually. Therefore,taking total flavonoids content for an index,it is suitable to harvest upper leaf of G. bicolor,upper leaf and stem of G. divaricata-1,and whole leaf of G. divaricata-2 in August.
