通过观察卵巢组织中不同发育阶段的各级卵泡的形态和数量,并结合促性腺激素和性激素的测定能够更好地评估卵巢功能。本研究目的在于改进现有的卵巢组织切片及染色方法,建立更快观察和评价卵巢组织中各级卵泡数量和质量的方法。取动情前...通过观察卵巢组织中不同发育阶段的各级卵泡的形态和数量,并结合促性腺激素和性激素的测定能够更好地评估卵巢功能。本研究目的在于改进现有的卵巢组织切片及染色方法,建立更快观察和评价卵巢组织中各级卵泡数量和质量的方法。取动情前期、动情期、动情后期和动情间期雌性C57BL/6J小鼠卵巢,用4%多聚甲醛固定,梯度蔗糖脱水,OCT(optimal cutting temperature compound)包埋,冰冻切片(厚7μm),快速H&E染色后进行观察。结果显示,本方法能够分辨次级卵泡、窦前、窦状和排卵前卵泡,虽然不能区分和分辨始基卵泡和初级卵泡,但是其它观察结果与复杂的方法相当。以上结果提示,快速和改进的小鼠卵巢冰冻切片和H&E染色方法可以与激素分析结合,用于小鼠模型中的卵巢发育和功能研究。展开更多
Objective:To compare the efficacy of three different tissue stains,namely haematoxylin and eosin(H&E),periodic-acid Schiff(PAS)and immunohistochemical(1HC)stains for detection of Entamoeba histolytica(E.histolytic...Objective:To compare the efficacy of three different tissue stains,namely haematoxylin and eosin(H&E),periodic-acid Schiff(PAS)and immunohistochemical(1HC)stains for detection of Entamoeba histolytica(E.histolytica)trophozoites in abscessed liver tissues of hamster.Methods:Amoebic liver abscess was experimentally induced in a hamster by injecting 1×10~6of axenically cultured virulent E.histolytica trophozoites(HM1-IMSS strain)into the portal vein.After a week post-inoculation,the hamster was sacrificed and the liver tissue sections were stained with H&E,PAS and IHC stains to delect the amoebic trophozoite.Results:The three stains revealed tissue necrosis and amoebic trophozoites,but with varying clarity.H&E and PAS stained the trophozoites pink and magenta,respectively,however it was difficult to differentiate the stained trophozoites from the macrophages because of their similarity in size and morphology.On the other hand,IHC stain revealed distinct brown appearance of the trophozoites in the infected liver tissues.Conclusions:It can be concluded that out of the three stains.IHC is the best for identification of E.histolytica trophozoites in tissue sections.展开更多
在基于Mesh-under的IPv6低功耗无线个域网(IPv6over low-power wireless personal area networks,6LoWPAN)中,针对传输路径上中间节点重传缓存溢出导致重传数据分片丢失,造成网络性能下降等问题,提出一种基于Mesh-under的备用缓存机制...在基于Mesh-under的IPv6低功耗无线个域网(IPv6over low-power wireless personal area networks,6LoWPAN)中,针对传输路径上中间节点重传缓存溢出导致重传数据分片丢失,造成网络性能下降等问题,提出一种基于Mesh-under的备用缓存机制。本文所提机制根据传输路径上各节点重传缓存使用情况及数据分片剩余跳数等信息,设置动态重传缓存门限,并为超过该门限的节点从其邻居节点中挑选合适的备用缓存节点,从而完成数据分片的缓存与重传过程,达到均衡使用各节点重传缓存的目的。结果表明,所提机制能够有效避免重传缓存溢出,减小网络能耗,同时进一步提高目的端重组成功率。展开更多
基金supported by Australian NHMRC and The University of Queensland
文摘通过观察卵巢组织中不同发育阶段的各级卵泡的形态和数量,并结合促性腺激素和性激素的测定能够更好地评估卵巢功能。本研究目的在于改进现有的卵巢组织切片及染色方法,建立更快观察和评价卵巢组织中各级卵泡数量和质量的方法。取动情前期、动情期、动情后期和动情间期雌性C57BL/6J小鼠卵巢,用4%多聚甲醛固定,梯度蔗糖脱水,OCT(optimal cutting temperature compound)包埋,冰冻切片(厚7μm),快速H&E染色后进行观察。结果显示,本方法能够分辨次级卵泡、窦前、窦状和排卵前卵泡,虽然不能区分和分辨始基卵泡和初级卵泡,但是其它观察结果与复杂的方法相当。以上结果提示,快速和改进的小鼠卵巢冰冻切片和H&E染色方法可以与激素分析结合,用于小鼠模型中的卵巢发育和功能研究。
基金Supported by a grant from Universiti Sains Malaysia(grant No.1001/PPSK/813009)received financial support through the USM Fellowship
文摘Objective:To compare the efficacy of three different tissue stains,namely haematoxylin and eosin(H&E),periodic-acid Schiff(PAS)and immunohistochemical(1HC)stains for detection of Entamoeba histolytica(E.histolytica)trophozoites in abscessed liver tissues of hamster.Methods:Amoebic liver abscess was experimentally induced in a hamster by injecting 1×10~6of axenically cultured virulent E.histolytica trophozoites(HM1-IMSS strain)into the portal vein.After a week post-inoculation,the hamster was sacrificed and the liver tissue sections were stained with H&E,PAS and IHC stains to delect the amoebic trophozoite.Results:The three stains revealed tissue necrosis and amoebic trophozoites,but with varying clarity.H&E and PAS stained the trophozoites pink and magenta,respectively,however it was difficult to differentiate the stained trophozoites from the macrophages because of their similarity in size and morphology.On the other hand,IHC stain revealed distinct brown appearance of the trophozoites in the infected liver tissues.Conclusions:It can be concluded that out of the three stains.IHC is the best for identification of E.histolytica trophozoites in tissue sections.
文摘在基于Mesh-under的IPv6低功耗无线个域网(IPv6over low-power wireless personal area networks,6LoWPAN)中,针对传输路径上中间节点重传缓存溢出导致重传数据分片丢失,造成网络性能下降等问题,提出一种基于Mesh-under的备用缓存机制。本文所提机制根据传输路径上各节点重传缓存使用情况及数据分片剩余跳数等信息,设置动态重传缓存门限,并为超过该门限的节点从其邻居节点中挑选合适的备用缓存节点,从而完成数据分片的缓存与重传过程,达到均衡使用各节点重传缓存的目的。结果表明,所提机制能够有效避免重传缓存溢出,减小网络能耗,同时进一步提高目的端重组成功率。
