A novel, simple, rapid, economic and hypersensitive method of measuring Cr3＋ is described in this paper. Cr3＋ could be oxidated to H2CrO6, which is extracted by n-C5H11OH, so a trace amount of chromium is measured d...A novel, simple, rapid, economic and hypersensitive method of measuring Cr3＋ is described in this paper. Cr3＋ could be oxidated to H2CrO6, which is extracted by n-C5H11OH, so a trace amount of chromium is measured directly by spectrometers, Sandra sensitivity is S=6.40×10^-3 ug.cm-2.展开更多
目的探讨高分辨率食管测压对于胃食管反流病食管运动功能诊断的应用价值。方法收集宁夏医科大学总医院2015年9月—2019年9月,伴有典型胃食管反流症状并经胃镜、24 h pH测定等相关检查诊断为胃食管反流病的患者共38例,收集无反流症状、...目的探讨高分辨率食管测压对于胃食管反流病食管运动功能诊断的应用价值。方法收集宁夏医科大学总医院2015年9月—2019年9月,伴有典型胃食管反流症状并经胃镜、24 h pH测定等相关检查诊断为胃食管反流病的患者共38例,收集无反流症状、胃镜及24 h pH测定未见明显异常的正常对照组共22例,分析比较其高分辨率食管测压相关指标。结果胃食管反流病患者食管下括约肌平均静息压(LESP)、远端收缩积分(DCI)、收缩前沿速度(CFV)与对照组比较差异均有统计学意义(P均<0.05)。GERD患者pH<4的总时间百分比、立位pH<4的时间百分比、卧位pH<4的时间百分比、pH<4的反流次数、最长反流持续时间、反流持续≥5 min次数、Demeester评分与对照组相比差异均有统计学意义(P均<0.05)。GERD患者食管下括约肌长度(LESL)、LESP、DCI、CFV、食团内部压力(IBP)、远端潜伏期(DL)指标中,部分患者存在单项或多项指标异常,总异常率78.95%,三项以上指标异常率39.47%。结论胃食管反流病患者存在食管下括约肌功能及食管体部运动功能异常,食管运动功能异常时高度提示抗反流屏障机制受损而存在病理性反流,高分辨率食管测压可以作为辅助检查用以明确GERD患者是否存在食管运动功能障碍。展开更多
A multiplexed targeted proteomic assay using a mTRAQ-MRM/MS-based approach was developed and assessed to systematically quantify the relative expressions of five candidate plasma apolipoproteins that have been previou...A multiplexed targeted proteomic assay using a mTRAQ-MRM/MS-based approach was developed and assessed to systematically quantify the relative expressions of five candidate plasma apolipoproteins that have been previously shown to be dysregulated in neuropsychiatric disorders and cognitive dysfunction:apolipoprotein H(APOH),apolipoprotein J(APOJ),apolipoprotein A4(APOA4),apolipoprotein E(APOE),and apolipoprotein D(APOD).The peptides and transitions of each APO were carefully selected according to the tandem MS signals acquired on a TripleTOFTM 5600,followed by optimization of the declustering potential and collision energy voltages for transitions on a QTRAP 5500.Our results showed that the collision energies of mTRAQ-labeled peptides were approximately 15%–20%higher than corresponding non-labeled peptides.Through optimized transitions and parameters,we analyzed the relative abundances of the five APOs in human plasma with and without depletion of high abundant proteins.The results indicated that the MRM signals of four target APOs were significantly increased after depletion,while the MRM signal of one APO,APOD,was decreased.Furthermore,the relative abundances of the five target APOs in healthy human plasma were stable,and the ranking of these proteins according to their MS responses changed slightly.Therefore,we deduced that the rank order of the MS signals for these target proteins can be developed as a diagnostic signature for diseased plasma.展开更多
文摘A novel, simple, rapid, economic and hypersensitive method of measuring Cr3＋ is described in this paper. Cr3＋ could be oxidated to H2CrO6, which is extracted by n-C5H11OH, so a trace amount of chromium is measured directly by spectrometers, Sandra sensitivity is S=6.40×10^-3 ug.cm-2.
文摘目的探讨高分辨率食管测压对于胃食管反流病食管运动功能诊断的应用价值。方法收集宁夏医科大学总医院2015年9月—2019年9月,伴有典型胃食管反流症状并经胃镜、24 h pH测定等相关检查诊断为胃食管反流病的患者共38例,收集无反流症状、胃镜及24 h pH测定未见明显异常的正常对照组共22例,分析比较其高分辨率食管测压相关指标。结果胃食管反流病患者食管下括约肌平均静息压(LESP)、远端收缩积分(DCI)、收缩前沿速度(CFV)与对照组比较差异均有统计学意义(P均<0.05)。GERD患者pH<4的总时间百分比、立位pH<4的时间百分比、卧位pH<4的时间百分比、pH<4的反流次数、最长反流持续时间、反流持续≥5 min次数、Demeester评分与对照组相比差异均有统计学意义(P均<0.05)。GERD患者食管下括约肌长度(LESL)、LESP、DCI、CFV、食团内部压力(IBP)、远端潜伏期(DL)指标中,部分患者存在单项或多项指标异常,总异常率78.95%,三项以上指标异常率39.47%。结论胃食管反流病患者存在食管下括约肌功能及食管体部运动功能异常,食管运动功能异常时高度提示抗反流屏障机制受损而存在病理性反流,高分辨率食管测压可以作为辅助检查用以明确GERD患者是否存在食管运动功能障碍。
基金supported by the National Basic Research Program of China(2009CB918300)the National Natural Science Foundation of China(31271189 and 81101009)
文摘A multiplexed targeted proteomic assay using a mTRAQ-MRM/MS-based approach was developed and assessed to systematically quantify the relative expressions of five candidate plasma apolipoproteins that have been previously shown to be dysregulated in neuropsychiatric disorders and cognitive dysfunction:apolipoprotein H(APOH),apolipoprotein J(APOJ),apolipoprotein A4(APOA4),apolipoprotein E(APOE),and apolipoprotein D(APOD).The peptides and transitions of each APO were carefully selected according to the tandem MS signals acquired on a TripleTOFTM 5600,followed by optimization of the declustering potential and collision energy voltages for transitions on a QTRAP 5500.Our results showed that the collision energies of mTRAQ-labeled peptides were approximately 15%–20%higher than corresponding non-labeled peptides.Through optimized transitions and parameters,we analyzed the relative abundances of the five APOs in human plasma with and without depletion of high abundant proteins.The results indicated that the MRM signals of four target APOs were significantly increased after depletion,while the MRM signal of one APO,APOD,was decreased.Furthermore,the relative abundances of the five target APOs in healthy human plasma were stable,and the ranking of these proteins according to their MS responses changed slightly.Therefore,we deduced that the rank order of the MS signals for these target proteins can be developed as a diagnostic signature for diseased plasma.
