Sedum alfredii Hance has been identified as zinc(Zn) and cadmium(Cd) co-hyperaccumulator.In this paper the relationships of Zn or Cd hyperaccumulation to the generation and the role of H2O2 in Sedum alfredii H.were ex...Sedum alfredii Hance has been identified as zinc(Zn) and cadmium(Cd) co-hyperaccumulator.In this paper the relationships of Zn or Cd hyperaccumulation to the generation and the role of H2O2 in Sedum alfredii H.were examined.The results show that Zn and Cd contents in the shoots of Sedum alfredii H.treated with 1000 μmol/L Zn2+ and/or 200 μmol/L Cd2+ increased linearly within 15 d.Contents of total S,glutathione(GSH) and H2O2 in shoots also increased within 15 d,and then decreased.Total S and GSH contents in shoots were higher under Cd2+ treatment than under Zn2+ treatment.However,reverse trends of H2O2 content in shoots were obtained,in which much higher H2O2 content was observed in Zn2+-treated shoots than in Cd2+-treated shoots.Similarly,the microscopic imaging of H2O2 accumulation in leaves using H2O2 probe technique showed that much higher H2O2 accumulation was observed in the Zn2+-treated leaf than in the Cd2+-treated one.These results suggest that there are different responses in the generation of H2O2 upon exposure to Zn2+ and Cd2+ for the hyperaccumulator Sedum alfredii H.And this is the first report that the generation of H2O2 may play an important role in Zn hyperaccumulation in the leaves.Our results also imply that GSH may play an important role in the detoxification of dissociated Zn/Cd and the generation of H2O2.展开更多
Taking annual Heracl'eum moellendorffii Hance as the material,this research studied the epidemic dynamics of H.moellendorffii Hance powdery mildew and plant growth by using the interactive methods of soil moisture...Taking annual Heracl'eum moellendorffii Hance as the material,this research studied the epidemic dynamics of H.moellendorffii Hance powdery mildew and plant growth by using the interactive methods of soil moisture and soil organic matter.The results showed that the interaction of higher soil moisture content(60%-80%)and soil organic matter content(75-100 g•kg^(-1))had few diseases,dosease index and area under disease progression curve(AUDPC)compared with those of other treatments,thus could effectivley control powdery mildew disease.Moreover,higher soil moisture and organic matter content increased the yield,contents of soluble sugar,soluble protein,vitamin C and coumarin in H.moellendorffii Hance.展开更多
The total flavonoid contents in cultural materials and natural plant of Hypericum sampsonii Hance were analysed and comparedwith that in cultural materials of H. perforatum Linn. The results showed that the total flav...The total flavonoid contents in cultural materials and natural plant of Hypericum sampsonii Hance were analysed and comparedwith that in cultural materials of H. perforatum Linn. The results showed that the total flavonoid contents reached 5.416%-5.868% in H.sampsonii calli cultured for 30 d, which werehigher than that ofnatural plantof thisspecies (4.325%-4.527%) and approached to cultural materials of H. perforatum .展开更多
基金Project (Nos. 30630046 and 20777068) supported by the National Natural Science Foundation of China
文摘Sedum alfredii Hance has been identified as zinc(Zn) and cadmium(Cd) co-hyperaccumulator.In this paper the relationships of Zn or Cd hyperaccumulation to the generation and the role of H2O2 in Sedum alfredii H.were examined.The results show that Zn and Cd contents in the shoots of Sedum alfredii H.treated with 1000 μmol/L Zn2+ and/or 200 μmol/L Cd2+ increased linearly within 15 d.Contents of total S,glutathione(GSH) and H2O2 in shoots also increased within 15 d,and then decreased.Total S and GSH contents in shoots were higher under Cd2+ treatment than under Zn2+ treatment.However,reverse trends of H2O2 content in shoots were obtained,in which much higher H2O2 content was observed in Zn2+-treated shoots than in Cd2+-treated shoots.Similarly,the microscopic imaging of H2O2 accumulation in leaves using H2O2 probe technique showed that much higher H2O2 accumulation was observed in the Zn2+-treated leaf than in the Cd2+-treated one.These results suggest that there are different responses in the generation of H2O2 upon exposure to Zn2+ and Cd2+ for the hyperaccumulator Sedum alfredii H.And this is the first report that the generation of H2O2 may play an important role in Zn hyperaccumulation in the leaves.Our results also imply that GSH may play an important role in the detoxification of dissociated Zn/Cd and the generation of H2O2.
基金Supported by the National Key Research and Development Program of China(2016YFC0500307-06)。
文摘Taking annual Heracl'eum moellendorffii Hance as the material,this research studied the epidemic dynamics of H.moellendorffii Hance powdery mildew and plant growth by using the interactive methods of soil moisture and soil organic matter.The results showed that the interaction of higher soil moisture content(60%-80%)and soil organic matter content(75-100 g•kg^(-1))had few diseases,dosease index and area under disease progression curve(AUDPC)compared with those of other treatments,thus could effectivley control powdery mildew disease.Moreover,higher soil moisture and organic matter content increased the yield,contents of soluble sugar,soluble protein,vitamin C and coumarin in H.moellendorffii Hance.
文摘The total flavonoid contents in cultural materials and natural plant of Hypericum sampsonii Hance were analysed and comparedwith that in cultural materials of H. perforatum Linn. The results showed that the total flavonoid contents reached 5.416%-5.868% in H.sampsonii calli cultured for 30 d, which werehigher than that ofnatural plantof thisspecies (4.325%-4.527%) and approached to cultural materials of H. perforatum .
