The nutritional values of different species of sea cucumber are greatly concerned because of their dietary and curative properties. In this study, two species of sea cucumber, Holothuria edulis, a low-valued noncommer...The nutritional values of different species of sea cucumber are greatly concerned because of their dietary and curative properties. In this study, two species of sea cucumber, Holothuria edulis, a low-valued noncommercial species, and Holothuria scabra, a high-valued commercial species were selected to compare its proximate composition and fatty acids. H. edulis a prevalent species in coastal water of Sabah is not commercially importance like H. scabra. Sea cucumbers were captured live from the Sabah marine habitat. All samples were immediately eviscerated, freeze-dried and stored at 4°C in until analyzed. Silylating agent N, O-Bis(trimethylsilyl) trifluoroacetamide (BSTFA) was used to derivitization of fatty acid prior to gas chromatography-mass spectrometry (GC-MS) analysis. Proximate compositions (%), such as moisture, crude protein, crude lipids and ash were carried using standard methods. Major fatty acids in H. edulis and H. scabra were saturated fatty acid (SFA) accounted for 83.95% and 98.60%, respectively and dominated with Palmitic acids. Polyunsaturated fatty acid (PUFA), arachidonic acid of 16.05% was found only in H. edulis, but absent H. scabra. Proximate compositions (dry weight) were varied greatly within these two species. Moisture, crude protein, crude lipids and ash of 85.5%, 70.5%, 1.37% and 1.27% respectively were obtained in H. edulis. On the other hand 84.5% of moisture, 51.2% of crude protein, 0.27% of crude lipids and 4.44% of ashes were determined in H. scabra. Significantly higher protein (p H. edulis compare to H. scabra could be the choice of option for the utilization of this non commercial species as nutraceutical industry and also alternatives to reduce the pressure on heavily exploited species of H. scabra.展开更多
A fragment with about 798 bp of cab gene was amplified from the first strand of Moso(Phyllostachys edulis)cDNA through RT-PCR method,named as cab-PhE4(cab gene 4 from Ph.edulis).The cab-PhE4(GenBank accession number:E...A fragment with about 798 bp of cab gene was amplified from the first strand of Moso(Phyllostachys edulis)cDNA through RT-PCR method,named as cab-PhE4(cab gene 4 from Ph.edulis).The cab-PhE4(GenBank accession number:EF405878)gene encodes 265 amino acid.The bioinformatics analysis indicated that the protein encoded by cab-PhE4 has a chlorophyll a/b-binding domain(64th-232nd position),a protein kinase C phosphorylation site(33rd-35th position),a N-myristoylation site(169th-174th position),and an involucrin repeat(207th-216th position).The amino acid sequence of cab-PhE4 showed high similarity with the cab genes of Zea mays,Triticum aestivum,Musa acuminata,Panax ginseng and Oryza sativa,more than 90%,respectively.展开更多
The light-harvesting chlorophyll a/b-protein complex plays an important role in photosynthesis of plants. A full-length cDNA of light-harvesting chlorophyll a/b （cab） gene was cloned from the first strand of Moso （...The light-harvesting chlorophyll a/b-protein complex plays an important role in photosynthesis of plants. A full-length cDNA of light-harvesting chlorophyll a/b （cab） gene was cloned from the first strand of Moso （Phyllostachys edulis） cDNA through RT-PCR and RACE methods, named as cabPhEIO （cab gene 10 from Ph. edulis）. The length of cab- PhEIO （GenBank accession number： EU118754） is 1 151 bp, which contains an open reading frame encoding 283 amino acids from 81st to 932nd position. The bioinformatics analysis indicated that the protein encoded by cab-PhElO had a chlorophll a/b binding domain （83rd -247th position）, two protein kinase C-phosphorylation sites, three Nmyristoylation sites and a yia A/B double helix domain.The amino acid sequence of cab-PhElO showed high similarity with the cab genes of Oryza sativa, Zea mays, Hordeum vulgare, and Vitis vinifera, more than 80%, respectively, which indicated that cab-PhElO gene belongs to lhcb5 gene family.展开更多
为了探究不同肥料对毛竹[Phyllostachys edulis(Carr.)H. de Lehaie]林出笋和新竹生长的影响,为毛竹林的精准施肥和科学经营提供理论依据。在江西省宜春市铜鼓县选择生长良好,具有代表性的毛竹纯林,在样地经垦复后,采用随机区组设计,设...为了探究不同肥料对毛竹[Phyllostachys edulis(Carr.)H. de Lehaie]林出笋和新竹生长的影响,为毛竹林的精准施肥和科学经营提供理论依据。在江西省宜春市铜鼓县选择生长良好,具有代表性的毛竹纯林,在样地经垦复后,采用随机区组设计,设置毛竹专用肥(ZYF)、矿渣肥(KZF)和不施肥(CK)处理,每个处理3次重复,共9块20 m×20 m的样地,研究不同肥料处理对毛竹出笋和新竹生长的影响。结果表明:两种肥料均在一定程度上提高了出笋数、退笋数和成竹数。毛竹专用肥处理出笋数和退笋数最高,分别较对照高出19.48%和51.17%,矿渣肥处理较对照处理分别高出16.05%和16.96%;而矿渣肥处理的成竹数最高,其次为毛竹专用肥处理,二者分别比对照高175、108株/hm^2。不同施肥处理间,竹笋地径总体呈毛竹专用肥处理最高,其次分别为矿渣肥和对照处理,而笋高在不同处理间的波动较小。2种肥料处理显著提高了新竹胸径和大径材毛竹的比例,并以毛竹专用肥处理效果更为明显。毛竹专用肥处理新竹枝下高显著高于对照处理,而矿渣肥与对照处理间无显著差异。结果表明毛竹专用肥更有利于作为以生产竹笋、大径材竹为目的的毛竹林培肥措施,矿渣肥对促进毛竹林优质、丰产的短期肥效低于毛竹专用肥,可能与其为缓释肥有关。展开更多
文摘The nutritional values of different species of sea cucumber are greatly concerned because of their dietary and curative properties. In this study, two species of sea cucumber, Holothuria edulis, a low-valued noncommercial species, and Holothuria scabra, a high-valued commercial species were selected to compare its proximate composition and fatty acids. H. edulis a prevalent species in coastal water of Sabah is not commercially importance like H. scabra. Sea cucumbers were captured live from the Sabah marine habitat. All samples were immediately eviscerated, freeze-dried and stored at 4°C in until analyzed. Silylating agent N, O-Bis(trimethylsilyl) trifluoroacetamide (BSTFA) was used to derivitization of fatty acid prior to gas chromatography-mass spectrometry (GC-MS) analysis. Proximate compositions (%), such as moisture, crude protein, crude lipids and ash were carried using standard methods. Major fatty acids in H. edulis and H. scabra were saturated fatty acid (SFA) accounted for 83.95% and 98.60%, respectively and dominated with Palmitic acids. Polyunsaturated fatty acid (PUFA), arachidonic acid of 16.05% was found only in H. edulis, but absent H. scabra. Proximate compositions (dry weight) were varied greatly within these two species. Moisture, crude protein, crude lipids and ash of 85.5%, 70.5%, 1.37% and 1.27% respectively were obtained in H. edulis. On the other hand 84.5% of moisture, 51.2% of crude protein, 0.27% of crude lipids and 4.44% of ashes were determined in H. scabra. Significantly higher protein (p H. edulis compare to H. scabra could be the choice of option for the utilization of this non commercial species as nutraceutical industry and also alternatives to reduce the pressure on heavily exploited species of H. scabra.
基金This project is supported by"948"introduction project(2004-4-60,2005-4-38)
文摘A fragment with about 798 bp of cab gene was amplified from the first strand of Moso(Phyllostachys edulis)cDNA through RT-PCR method,named as cab-PhE4(cab gene 4 from Ph.edulis).The cab-PhE4(GenBank accession number:EF405878)gene encodes 265 amino acid.The bioinformatics analysis indicated that the protein encoded by cab-PhE4 has a chlorophyll a/b-binding domain(64th-232nd position),a protein kinase C phosphorylation site(33rd-35th position),a N-myristoylation site(169th-174th position),and an involucrin repeat(207th-216th position).The amino acid sequence of cab-PhE4 showed high similarity with the cab genes of Zea mays,Triticum aestivum,Musa acuminata,Panax ginseng and Oryza sativa,more than 90%,respectively.
基金This project is supported by "948" Introduction Project (2004-4-60, 2005-4-38)
文摘The light-harvesting chlorophyll a/b-protein complex plays an important role in photosynthesis of plants. A full-length cDNA of light-harvesting chlorophyll a/b （cab） gene was cloned from the first strand of Moso （Phyllostachys edulis） cDNA through RT-PCR and RACE methods, named as cabPhEIO （cab gene 10 from Ph. edulis）. The length of cab- PhEIO （GenBank accession number： EU118754） is 1 151 bp, which contains an open reading frame encoding 283 amino acids from 81st to 932nd position. The bioinformatics analysis indicated that the protein encoded by cab-PhElO had a chlorophll a/b binding domain （83rd -247th position）, two protein kinase C-phosphorylation sites, three Nmyristoylation sites and a yia A/B double helix domain.The amino acid sequence of cab-PhElO showed high similarity with the cab genes of Oryza sativa, Zea mays, Hordeum vulgare, and Vitis vinifera, more than 80%, respectively, which indicated that cab-PhElO gene belongs to lhcb5 gene family.
