The F3′5′H gene family plays an important role in regulating anthocyanin biosynthesis,abiotic stress,and hormone signaling.In this study,14 F3′5′H genes were identified from the blueberry genome.The chromosomal di...The F3′5′H gene family plays an important role in regulating anthocyanin biosynthesis,abiotic stress,and hormone signaling.In this study,14 F3′5′H genes were identified from the blueberry genome.The chromosomal distribution,physicochemical properties,F3′5′H domain,conserved motifs,cis-acting elements,and intron/exon compositions were analyzed.The functional prediction analysis of these VcF3′5′Hs indicated that their biological functions included light response and other secondary metabolites.The results of qRT-PCR showed that VcF3′5′Hs(especially VcF3′5′H4)were highly expressed at the ripening stage.Subcellular localization revealed that VcF3′5′H4 may be located in the endoplasmic reticulum.Co-expression analysis showed that the VcF3′5′H gene family was related to anthocyanin.This research provides an overview of the blueberry F3′5′H family and helps verify the role of these genes in regulating anthocyanin biosynthesis.展开更多
利用SMART(switching mechanism at 5’end of the RNA transcript)技术构建了红肉猕猴桃品种‘红阳’(Actinidia chinesis cv‘Hongyang’)内果皮组织的全长cDNA文库,此文库的构建有助于克隆与次生代谢相关的基因,特别是红肉猕...利用SMART(switching mechanism at 5’end of the RNA transcript)技术构建了红肉猕猴桃品种‘红阳’(Actinidia chinesis cv‘Hongyang’)内果皮组织的全长cDNA文库,此文库的构建有助于克隆与次生代谢相关的基因,特别是红肉猕猴桃花青素特异合成代谢的基因。文库滴度为6.7×10^4cfu/mL,库容为2.72×10^8cfu/mL,文库重组率99.8%,插入片段多数分布在700~1000bp。随机挑选1014个克隆进行测序,测序成功963个,经过序列拼接去除低质量序列后获得632个unigenes,包括92个contigs和540个singletons,获得已知功能unigenes共441个。从所测克隆中得到一个花青素途径的结构基因AcF3H,其cDNA序列长1369bp(GenBank登录号:FJ542819),CDS区为1101bp,编码366个氨基酸的多肽。与拟南芥、葡萄及龙胆已知乃日氨基酸序列比对,发现该基因十分保守。通过RT—PCR技术对苍溪栽培的不同发育时期‘红阳’猕猴桃果实AcF3H基因的表达进行了分析。结果表明,果肉转色前AcF3H基因表达量较高,而转色初期表达量降低,此后随着果实着色加深表达量维持在较高水平。展开更多
基金funded by the National Natural Science Foundation of China(Grant No.31760205)Guangdong Basic and Applied Basic Research Foundation of Guangdong Province-Yuehui Joint Foundation(Grant No.2022A1515111095)+1 种基金the Professor and Doctoral Research Project(Grant No.2022JB021)in Huizhou Universitythe Project of the Department of Education of Guangdong Province(Grant No.2018KTSCX214).
文摘The F3′5′H gene family plays an important role in regulating anthocyanin biosynthesis,abiotic stress,and hormone signaling.In this study,14 F3′5′H genes were identified from the blueberry genome.The chromosomal distribution,physicochemical properties,F3′5′H domain,conserved motifs,cis-acting elements,and intron/exon compositions were analyzed.The functional prediction analysis of these VcF3′5′Hs indicated that their biological functions included light response and other secondary metabolites.The results of qRT-PCR showed that VcF3′5′Hs(especially VcF3′5′H4)were highly expressed at the ripening stage.Subcellular localization revealed that VcF3′5′H4 may be located in the endoplasmic reticulum.Co-expression analysis showed that the VcF3′5′H gene family was related to anthocyanin.This research provides an overview of the blueberry F3′5′H family and helps verify the role of these genes in regulating anthocyanin biosynthesis.
文摘利用SMART(switching mechanism at 5’end of the RNA transcript)技术构建了红肉猕猴桃品种‘红阳’(Actinidia chinesis cv‘Hongyang’)内果皮组织的全长cDNA文库,此文库的构建有助于克隆与次生代谢相关的基因,特别是红肉猕猴桃花青素特异合成代谢的基因。文库滴度为6.7×10^4cfu/mL,库容为2.72×10^8cfu/mL,文库重组率99.8%,插入片段多数分布在700~1000bp。随机挑选1014个克隆进行测序,测序成功963个,经过序列拼接去除低质量序列后获得632个unigenes,包括92个contigs和540个singletons,获得已知功能unigenes共441个。从所测克隆中得到一个花青素途径的结构基因AcF3H,其cDNA序列长1369bp(GenBank登录号:FJ542819),CDS区为1101bp,编码366个氨基酸的多肽。与拟南芥、葡萄及龙胆已知乃日氨基酸序列比对,发现该基因十分保守。通过RT—PCR技术对苍溪栽培的不同发育时期‘红阳’猕猴桃果实AcF3H基因的表达进行了分析。结果表明,果肉转色前AcF3H基因表达量较高,而转色初期表达量降低,此后随着果实着色加深表达量维持在较高水平。