Nano-hydroxyapatite/poly（e-caprolactone） （nHA/PCL） composite materials are among the best candidates for application in bone tissue engineering. As the main technique to fabricate porous scaffolds, electrospilming...Nano-hydroxyapatite/poly（e-caprolactone） （nHA/PCL） composite materials are among the best candidates for application in bone tissue engineering. As the main technique to fabricate porous scaffolds, electrospilming produce scaffolds with unsatisfactory mechanical strength and limited pore size for cdi infiltration. Micron-sized fiber assembly with higher mechanical strength is qualified to structure hybrid scaffolds. In this study, nHA/PCL monofilament fibers with different mass ratios were fabricated through melt-spinning. Transmission electron microscope （TEM） was used to observe the aggregation between nHA parfides. Other characterizations including scanning electron microscopy （SEM）, attenuated total reflection Fourier transform infrared spectroscopy （ATR.FTIR） and X-ray diffraction （XRD） were done to discuss the morphology, components and crystallization of the nHA/PCL composite fibers, respectively. The influence of nHA/PCL mass ratio on the tensile properties and water contact angle of composite fibers was also studied. The SEM images show the homogeneous dispersion of nano partides in the polymer matrix. Besides, nHA content increases the tensile strength, initial modulus and hydrophillcity of the composite fibers under the premise of spinnability. This kind of fibers is strong enough to fabricate fiber assembly which may have potential application in bone tissue engineering.展开更多
The resection of malignant osteosarcoma often results in large segmental bone defects,and the residual cells can facilitate recurrence.Consequently,the treatment of osteosarcoma is a major challenge in clinical practi...The resection of malignant osteosarcoma often results in large segmental bone defects,and the residual cells can facilitate recurrence.Consequently,the treatment of osteosarcoma is a major challenge in clinical practice.The ideal goal of treatment for osteosarcoma is to eliminate it thoroughly,and repair the resultant bone defects as well as avoid bacterial infections.Herein,we fabricated a selenium/strontium/zinc-doped hydroxyapatite(Se/Sr/Zn-HA)powder by hydrothermal method,and then employed it with polycaprolactone(PCL)as ink to construct composite scaffolds through 3D printing,and finally introduced them in bone defect repair induced by malignant osteosarcoma.The resultant composite scaffolds integrated multiple functions involving anti-tumor,osteogenic,and antibacterial potentials,mainly attributed to the anti-tumor effects of SeO_(3)^(2-),osteogenic ef-fects of Sr^(2+)and Zn^(2+),and antibacterial effects of SeO_(3)^(2-)and Zn^(2+).In vitro studies confirmed that Se/Sr/Zn-HA leaching solution could induce apoptosis of osteosarcoma cells,differentiation of MSCs,and proliferation of MC3T3-E1 while showing excellent antibacterial properties.In vivo tests demonstrated that Se/Sr/Zn-HA could significantly suppress tumors after 8 days of injection,and the Se/Sr/Zn-HA-PCLs scaffold repaired femoral defects effectively after 3 months of implantation.Summarily,the Se/Sr/Zn-HA-PCLs composite scaffolds developed in this study were effective for tumor treatment,bone defect repair,and post-operative anti-infection,which provided a great potential to be a facile therapeutic material for osteosarcoma resection.展开更多
基金Science and Technology Support Program of Shanghai,China(No.16441903803)National Postdoctoral Foundation,China(No.2016M590299)
文摘Nano-hydroxyapatite/poly（e-caprolactone） （nHA/PCL） composite materials are among the best candidates for application in bone tissue engineering. As the main technique to fabricate porous scaffolds, electrospilming produce scaffolds with unsatisfactory mechanical strength and limited pore size for cdi infiltration. Micron-sized fiber assembly with higher mechanical strength is qualified to structure hybrid scaffolds. In this study, nHA/PCL monofilament fibers with different mass ratios were fabricated through melt-spinning. Transmission electron microscope （TEM） was used to observe the aggregation between nHA parfides. Other characterizations including scanning electron microscopy （SEM）, attenuated total reflection Fourier transform infrared spectroscopy （ATR.FTIR） and X-ray diffraction （XRD） were done to discuss the morphology, components and crystallization of the nHA/PCL composite fibers, respectively. The influence of nHA/PCL mass ratio on the tensile properties and water contact angle of composite fibers was also studied. The SEM images show the homogeneous dispersion of nano partides in the polymer matrix. Besides, nHA content increases the tensile strength, initial modulus and hydrophillcity of the composite fibers under the premise of spinnability. This kind of fibers is strong enough to fabricate fiber assembly which may have potential application in bone tissue engineering.
基金supported by the National Natural Science Foundation of China(52071277,21802009)Jiangsu Provincial Key Research and Development Program(CN)(BE2019608)+4 种基金China Postdoctoral Science Foundation(2022M721685)Jiangsu Health Commission Medical Research Program(2020158)National Facility for Translational Med-icine(Shanghai)Open Program(TMSK-2021-304)2020 Nanjing In-ternational Science and Technology Cooperation ProgramNanjing Medical Science and Technology Development Key Program(ZKX18041).
文摘The resection of malignant osteosarcoma often results in large segmental bone defects,and the residual cells can facilitate recurrence.Consequently,the treatment of osteosarcoma is a major challenge in clinical practice.The ideal goal of treatment for osteosarcoma is to eliminate it thoroughly,and repair the resultant bone defects as well as avoid bacterial infections.Herein,we fabricated a selenium/strontium/zinc-doped hydroxyapatite(Se/Sr/Zn-HA)powder by hydrothermal method,and then employed it with polycaprolactone(PCL)as ink to construct composite scaffolds through 3D printing,and finally introduced them in bone defect repair induced by malignant osteosarcoma.The resultant composite scaffolds integrated multiple functions involving anti-tumor,osteogenic,and antibacterial potentials,mainly attributed to the anti-tumor effects of SeO_(3)^(2-),osteogenic ef-fects of Sr^(2+)and Zn^(2+),and antibacterial effects of SeO_(3)^(2-)and Zn^(2+).In vitro studies confirmed that Se/Sr/Zn-HA leaching solution could induce apoptosis of osteosarcoma cells,differentiation of MSCs,and proliferation of MC3T3-E1 while showing excellent antibacterial properties.In vivo tests demonstrated that Se/Sr/Zn-HA could significantly suppress tumors after 8 days of injection,and the Se/Sr/Zn-HA-PCLs scaffold repaired femoral defects effectively after 3 months of implantation.Summarily,the Se/Sr/Zn-HA-PCLs composite scaffolds developed in this study were effective for tumor treatment,bone defect repair,and post-operative anti-infection,which provided a great potential to be a facile therapeutic material for osteosarcoma resection.
