目的将HA1077联合自体MSCs治疗慢性皮肤溃疡创面,观察能否促进创面愈合。方法体外分离人MSCs。慢性皮肤创面病人4例,15个创面,随机分为对照组、HA1077组、细胞移植组。对照组,基本外科换药治疗。HA1077组创面局部外用0.5 ml HA1077注射...目的将HA1077联合自体MSCs治疗慢性皮肤溃疡创面,观察能否促进创面愈合。方法体外分离人MSCs。慢性皮肤创面病人4例,15个创面,随机分为对照组、HA1077组、细胞移植组。对照组,基本外科换药治疗。HA1077组创面局部外用0.5 ml HA1077注射液。细胞治疗组除HA1077治疗外,还局部应用骨髓间充质干细胞。治疗后3、7、14 d计算愈合指数（WCI）。结果应用HA1077复合MSCs细胞组,在14 d时的创面愈合指数为36.32±9.92,效果好于单纯应用HA1077组和对照组,而HA1077组与对照组差别不大。结论HA1077可促进MSCs细胞修复慢性皮肤创面。展开更多
AIM：To investigate the effects of dexamethasone（DEX） and 1-（5-isoquinolinesulfonyl）-homopiperazine（HA1077） on actin cytoskeleton and β-catenin in cultured human trabecular meshwork（HTM） cells.METHODS： The H...AIM：To investigate the effects of dexamethasone（DEX） and 1-（5-isoquinolinesulfonyl）-homopiperazine（HA1077） on actin cytoskeleton and β-catenin in cultured human trabecular meshwork（HTM） cells.METHODS： The HTM cells were separated from human eyeball and cultured in vitro.They were divided into control group,DEX（1×10^-6mol/L） group,HA1077（3×10^-5mol/L）group,and DEX（1×10^-6mol/L） and HA1077（3×10^-5mol/L）group.Actin cytoskeleton and β-catenin in HTM cells of the four groups were examined by immunofluorescence and Western blot analyses.RESULTS： In DEX group,there were reorganization of actin cytoskeleton and formation of cross linked actin networks（CLANs）,which were partially reversed in DEX and HA1077 group.DEX treatment also induced an increased expression of β-catenin,which was obviously reduced in DEX and HA1077 group.Meanwhile,the cultured HTM cells in HA1077 group had lower expression of β-catenin than that in the control group. CONCLUSION： Our results show that HA1077 can reverse the changes of actin organization and expression of β-catenin induced by DEX in cultured HTM cells,suggesting that HA1077 may play an important role in increasing outflow and reducing intraocular pressure.展开更多
文摘目的将HA1077联合自体MSCs治疗慢性皮肤溃疡创面,观察能否促进创面愈合。方法体外分离人MSCs。慢性皮肤创面病人4例,15个创面,随机分为对照组、HA1077组、细胞移植组。对照组,基本外科换药治疗。HA1077组创面局部外用0.5 ml HA1077注射液。细胞治疗组除HA1077治疗外,还局部应用骨髓间充质干细胞。治疗后3、7、14 d计算愈合指数（WCI）。结果应用HA1077复合MSCs细胞组,在14 d时的创面愈合指数为36.32±9.92,效果好于单纯应用HA1077组和对照组,而HA1077组与对照组差别不大。结论HA1077可促进MSCs细胞修复慢性皮肤创面。
基金Supported by the Natural Science Foundation of China (No.81300768 No.81371048+4 种基金 No.81670853)Science and Technology Department of Sichuan Province of China (No.2015HH0031)Health and Family Planning Commission of Sichuan Province of China (No.100539 No. 090505 No.090514)
文摘AIM：To investigate the effects of dexamethasone（DEX） and 1-（5-isoquinolinesulfonyl）-homopiperazine（HA1077） on actin cytoskeleton and β-catenin in cultured human trabecular meshwork（HTM） cells.METHODS： The HTM cells were separated from human eyeball and cultured in vitro.They were divided into control group,DEX（1×10^-6mol/L） group,HA1077（3×10^-5mol/L）group,and DEX（1×10^-6mol/L） and HA1077（3×10^-5mol/L）group.Actin cytoskeleton and β-catenin in HTM cells of the four groups were examined by immunofluorescence and Western blot analyses.RESULTS： In DEX group,there were reorganization of actin cytoskeleton and formation of cross linked actin networks（CLANs）,which were partially reversed in DEX and HA1077 group.DEX treatment also induced an increased expression of β-catenin,which was obviously reduced in DEX and HA1077 group.Meanwhile,the cultured HTM cells in HA1077 group had lower expression of β-catenin than that in the control group. CONCLUSION： Our results show that HA1077 can reverse the changes of actin organization and expression of β-catenin induced by DEX in cultured HTM cells,suggesting that HA1077 may play an important role in increasing outflow and reducing intraocular pressure.
