建立了分散固相萃取-超高效液相色谱-串联质谱法准确测定城市污水中六溴环十二烷(HBCD)和三-(2,3-二溴丙基)异氰脲酸酯(TBC)的方法.对萃取剂用量、萃取时间、淋洗剂、洗脱剂及色谱分离和质谱测定等条件进行了优化.优化后方法为:待测水...建立了分散固相萃取-超高效液相色谱-串联质谱法准确测定城市污水中六溴环十二烷(HBCD)和三-(2,3-二溴丙基)异氰脲酸酯(TBC)的方法.对萃取剂用量、萃取时间、淋洗剂、洗脱剂及色谱分离和质谱测定等条件进行了优化.优化后方法为:待测水样加入同位素标记的回收率内标,用0.4 g Silica、C18和HLB(1∶1∶1)混合填料分散固相萃取,回收萃取剂后用5 m L的10%的甲醇水溶液淋洗,8 m L甲醇洗脱,氮吹浓缩,加入同位素标记的进样内标测定.方法在1—200μg·L-1范围线性关系良好,HBCD和TBC的线性相关系数R2>0.9986,检出限为0.6—1.2 ng·L-1.应用该方法在某大型污水处理厂各工艺段污水中检测出了α-HBCD,浓度水平为1.3—5.4 ng·L-1.该方法操作简单、灵敏度高,尤其适合城市污水复杂基质中HBCD和TBC的分析.展开更多
To investigate the genotoxicity and reveal the potential toxicological mechanisms of Hexabromocyclododecane （HBCD）, human breast cells HBL-100 were exposed to a sequence of HBCD concentrations （0, 5, 10, and 50 mg/...To investigate the genotoxicity and reveal the potential toxicological mechanisms of Hexabromocyclododecane （HBCD）, human breast cells HBL-100 were exposed to a sequence of HBCD concentrations （0, 5, 10, and 50 mg/L） for 24 h. With a series of zymology and molecular biology methods, we found that HBCD induced dose-dependent oxidative stress on HBL-100 DNA. As revealed in q RT-PCR, activated prognostic factor ATM down-regulated tumor suppressor gene BRCA1 and prompted DNA repair genes h OGG1 and h MTH1 expression in lower concentrations of HBCD （〈 10 mg/L）. However, DNA repair were inhibited as well as cell proliferation rate by higher concentrations of HBCD （50 mg/L）. The results inferred that the genotoxicity of HBCD was dose-dependent and related to DNA repair pathway.展开更多
为了解六溴环十二烷(HBCDs)在海洋沉积物中的好氧微生物降解特性,在以HBCDs为唯一碳源的无机培养基中添加海洋沉积物悬浮液,利用HPLC-MS/MS测定HBCDs降解率,并驯化分离得到HBCDs降解菌株,进行菌种鉴定.结果显示:海洋沉积物好氧微生物对H...为了解六溴环十二烷(HBCDs)在海洋沉积物中的好氧微生物降解特性,在以HBCDs为唯一碳源的无机培养基中添加海洋沉积物悬浮液,利用HPLC-MS/MS测定HBCDs降解率,并驯化分离得到HBCDs降解菌株,进行菌种鉴定.结果显示:海洋沉积物好氧微生物对HBCDs的降解半衰期为38-61 d;在120 d中HBCDs共降解了72%;从海洋沉积物中富集、分离得到5株能够以HBCDs为唯一碳源的降解菌株,分别命名为CTF711-A、CTF711-C、CTF711-D、CTF711-E和CTF711-X,结合形态学观察、生理生化试验和16S r DNA序列分析,将其依次鉴别为假单胞菌属、红球菌属、不动杆菌属、微杆菌属和盐单胞菌属,除假单胞菌属外,其余菌株对HBCDs的降解能力还尚未见报道.同时将这5株菌分别培养与混合培养,混合菌株对HBCDs降解率最高.本研究报道了5株不同属的HBCDs好氧降解菌株,且混合菌株对HBCDs降解效果较好,结果可为HBCDs的生物修复提供理论和应用依据.展开更多
文摘建立了分散固相萃取-超高效液相色谱-串联质谱法准确测定城市污水中六溴环十二烷(HBCD)和三-(2,3-二溴丙基)异氰脲酸酯(TBC)的方法.对萃取剂用量、萃取时间、淋洗剂、洗脱剂及色谱分离和质谱测定等条件进行了优化.优化后方法为:待测水样加入同位素标记的回收率内标,用0.4 g Silica、C18和HLB(1∶1∶1)混合填料分散固相萃取,回收萃取剂后用5 m L的10%的甲醇水溶液淋洗,8 m L甲醇洗脱,氮吹浓缩,加入同位素标记的进样内标测定.方法在1—200μg·L-1范围线性关系良好,HBCD和TBC的线性相关系数R2>0.9986,检出限为0.6—1.2 ng·L-1.应用该方法在某大型污水处理厂各工艺段污水中检测出了α-HBCD,浓度水平为1.3—5.4 ng·L-1.该方法操作简单、灵敏度高,尤其适合城市污水复杂基质中HBCD和TBC的分析.
基金supported by the National Natural Science Foundation of China(No.41406088)The open fund of Key Laboratory for Ecological Environment in Coastal Areas,State Oceanic Administration(201506)
文摘To investigate the genotoxicity and reveal the potential toxicological mechanisms of Hexabromocyclododecane （HBCD）, human breast cells HBL-100 were exposed to a sequence of HBCD concentrations （0, 5, 10, and 50 mg/L） for 24 h. With a series of zymology and molecular biology methods, we found that HBCD induced dose-dependent oxidative stress on HBL-100 DNA. As revealed in q RT-PCR, activated prognostic factor ATM down-regulated tumor suppressor gene BRCA1 and prompted DNA repair genes h OGG1 and h MTH1 expression in lower concentrations of HBCD （〈 10 mg/L）. However, DNA repair were inhibited as well as cell proliferation rate by higher concentrations of HBCD （50 mg/L）. The results inferred that the genotoxicity of HBCD was dose-dependent and related to DNA repair pathway.
文摘为了解六溴环十二烷(HBCDs)在海洋沉积物中的好氧微生物降解特性,在以HBCDs为唯一碳源的无机培养基中添加海洋沉积物悬浮液,利用HPLC-MS/MS测定HBCDs降解率,并驯化分离得到HBCDs降解菌株,进行菌种鉴定.结果显示:海洋沉积物好氧微生物对HBCDs的降解半衰期为38-61 d;在120 d中HBCDs共降解了72%;从海洋沉积物中富集、分离得到5株能够以HBCDs为唯一碳源的降解菌株,分别命名为CTF711-A、CTF711-C、CTF711-D、CTF711-E和CTF711-X,结合形态学观察、生理生化试验和16S r DNA序列分析,将其依次鉴别为假单胞菌属、红球菌属、不动杆菌属、微杆菌属和盐单胞菌属,除假单胞菌属外,其余菌株对HBCDs的降解能力还尚未见报道.同时将这5株菌分别培养与混合培养,混合菌株对HBCDs降解率最高.本研究报道了5株不同属的HBCDs好氧降解菌株,且混合菌株对HBCDs降解效果较好,结果可为HBCDs的生物修复提供理论和应用依据.
