Naturally occurring mutations in surface proteins of Hepatitis B virus(HBV) usually result in altered hepatitis B surface antigen(HBsAg) secretion efficiency.In the present study,we reported two conserved residues,M75...Naturally occurring mutations in surface proteins of Hepatitis B virus(HBV) usually result in altered hepatitis B surface antigen(HBsAg) secretion efficiency.In the present study,we reported two conserved residues,M75 and M103 with respect to HBsAg,mutations of which not only attenuated HBsAg secretion(M75 only),but also suppressed HBV genome replication without compromising the overlapping p-gene product.We also found M75 and M103 can initiate truncated surface protein(TSPs) synthesis upon over-expression of full-length surface proteins,which may possibly contribute to HBV genome replication.However,attempts to rescue replicationdefective HBV mutant by co-expression of TSPs initiated from M75 or M103 were unsuccessful,which indicated surface proteins rather than the putative TSPs were involved in regulation of HBV genome replication.展开更多
目的:探讨低水平HBV-DNA乙肝患者乙肝病毒外膜大蛋白(hepatitis B virus large surface protein,LHBs)的表达及其意义,从而探讨LHBs是否可以用作乙型肝炎e抗原(hepatitis B e antigen,HBeAg)阴性乙型肝炎患者病毒复制程度的判定指标。方...目的:探讨低水平HBV-DNA乙肝患者乙肝病毒外膜大蛋白(hepatitis B virus large surface protein,LHBs)的表达及其意义,从而探讨LHBs是否可以用作乙型肝炎e抗原(hepatitis B e antigen,HBeAg)阴性乙型肝炎患者病毒复制程度的判定指标。方法:对83例HBeAg阴性的慢性乙型肝炎患者血清,采用ELISA进行LHBs检测,全自动免疫分析仪检测HBeAg,实时荧光定量PCR方法检测HBV DNA。分析HBV DNA无拷贝数组、低拷贝数组及高拷贝数组中LHBs的检出率以及HBV DNA不同拷贝数组与LHBs的相关性。结果:在HBV DNA无拷贝数组,LHBs的阳性率为6.38%;低拷贝数组,LHBs的阳性率为56.25%,吸光度值与拷贝数对数值没有显著的相关性(r=0.25,P=0.36);高拷贝数组,阳性率为95%,吸光度值与拷贝数对数值有良好相关性(r=0.90,P<0.0001)。结论:检测HBeAg阴性慢性乙型肝炎患者血清LHBs,有助于判断患者体内HBV的复制程度,但是否能够作为HBeAg阴性乙型肝炎抗病毒治疗终点的有效判定指标,尚有待进一步探讨。展开更多
基金supported by the National Basic Research Program of China(2007CB512900)
文摘Naturally occurring mutations in surface proteins of Hepatitis B virus(HBV) usually result in altered hepatitis B surface antigen(HBsAg) secretion efficiency.In the present study,we reported two conserved residues,M75 and M103 with respect to HBsAg,mutations of which not only attenuated HBsAg secretion(M75 only),but also suppressed HBV genome replication without compromising the overlapping p-gene product.We also found M75 and M103 can initiate truncated surface protein(TSPs) synthesis upon over-expression of full-length surface proteins,which may possibly contribute to HBV genome replication.However,attempts to rescue replicationdefective HBV mutant by co-expression of TSPs initiated from M75 or M103 were unsuccessful,which indicated surface proteins rather than the putative TSPs were involved in regulation of HBV genome replication.
文摘目的:探讨低水平HBV-DNA乙肝患者乙肝病毒外膜大蛋白(hepatitis B virus large surface protein,LHBs)的表达及其意义,从而探讨LHBs是否可以用作乙型肝炎e抗原(hepatitis B e antigen,HBeAg)阴性乙型肝炎患者病毒复制程度的判定指标。方法:对83例HBeAg阴性的慢性乙型肝炎患者血清,采用ELISA进行LHBs检测,全自动免疫分析仪检测HBeAg,实时荧光定量PCR方法检测HBV DNA。分析HBV DNA无拷贝数组、低拷贝数组及高拷贝数组中LHBs的检出率以及HBV DNA不同拷贝数组与LHBs的相关性。结果:在HBV DNA无拷贝数组,LHBs的阳性率为6.38%;低拷贝数组,LHBs的阳性率为56.25%,吸光度值与拷贝数对数值没有显著的相关性(r=0.25,P=0.36);高拷贝数组,阳性率为95%,吸光度值与拷贝数对数值有良好相关性(r=0.90,P<0.0001)。结论:检测HBeAg阴性慢性乙型肝炎患者血清LHBs,有助于判断患者体内HBV的复制程度,但是否能够作为HBeAg阴性乙型肝炎抗病毒治疗终点的有效判定指标,尚有待进一步探讨。
