Specific nucleotide variations in the E2 DNA sequence were looked for in samples with an intact human papillomavirus (HPV) 16 episomal E2 DNA. Ninety two women, 76 with invasive cervical carcinoma and 16 with cervical...Specific nucleotide variations in the E2 DNA sequence were looked for in samples with an intact human papillomavirus (HPV) 16 episomal E2 DNA. Ninety two women, 76 with invasive cervical carcinoma and 16 with cervical intraepithelial neoplasia (CIN) were recruited. HPV DNA typing was performed by polymerase chain reaction (PCR) based restriction fragment length polymorphism (RFLP). Intact episomal E2 DNA of HPV 16 was detected by PCR. Important nucleotide variations in samples with amplifiable E2DNA were detected by RFLP. Nucleotide sequencing was performed on representative samples to confirm RFLP findings. A total of 89 (96.7% ) women were positive for HPV DNA. Of these, 56 (63% ) were positive for HPV 16, and of these, 38 (68% ) were positive for intact episomal HPV 16 E2 DNA while 18 (32% )- were negative. Samples with intact episomal HPV 16 E2DNA sequences were grouped into four different digestion profiles I to IV based on RFLP patterns. Digestion patterns revealed absence of any sequence variations in samples with digestion profile I and presence of a 2983 A- G variation in those with profile II. Samples with digestion profiles III and IV revealed three variations in the hinge region (3516 C- A, 3538 A- C, 3566 T- G) and two in the DNA binding domain (3684 C- A, 3694 T- A) of the E2 sequence. Sequencing performed on representative samples confirmed RFLP findings. PCR RFLP helped in the identification of important HPV 16 E2 sequence variations, circumventing the need for sequencing. The presence of the nucleotide variations in positions that could alter the biological and immunological functions of the E2 protein combined with its increased occurrence in this study bring out the importance of these variations.展开更多
目的研究深圳城区育龄妇女与高危型人乳头瘤病毒(high risk human papillomavirus,HR-HPV)感染率和宫颈糜烂患病率的年龄分布特点及相关性。方法 2004年3月～2008年3月期间对深圳市城区育龄妇女1943例进行宫颈癌筛查,包括问卷调查、妇...目的研究深圳城区育龄妇女与高危型人乳头瘤病毒(high risk human papillomavirus,HR-HPV)感染率和宫颈糜烂患病率的年龄分布特点及相关性。方法 2004年3月～2008年3月期间对深圳市城区育龄妇女1943例进行宫颈癌筛查,包括问卷调查、妇科检查,收集宫颈脱落细胞。采用第二代杂交捕获(HC-2)法检测13种高危型HPV。结果深圳城区妇女HR-HPV DNA阳性381例,阳性率19.6%。各年龄组感染率之间有显著性差异(χ2=28.67,P<0.01)。宫颈糜烂患病率为41.8%,从年龄分布来看,不同年龄组间宫颈糜烂患病率有显著性差异(χ2=81.08,P<0.001)。其中患病率最高的是25～29岁组55.2%。在各年龄组轻度及中度宫颈糜烂患病率差异无显著性(P>0.05)。但在重度宫颈糜烂百分比中25～29岁组重度糜烂患病率差异有显著性(P<0.05)。宫颈光滑、宫颈糜烂患者的HR-HPV感染率分别是15.8%及24.8%,宫颈糜烂患者感染HR-HPV的OR值为1.569,95%CI(1.310～1.878)。结论①深圳城区妇女生殖道HR-HPV感染的年龄分布存在着两个感染高峰年龄段(25～29岁及55～60岁),宫颈糜烂患病率存在一个患病高峰年龄段(25～29岁);②25～29岁组是患重度宫颈糜烂的高发年龄段;③宫颈糜烂是年轻女性感染生殖道HR-HPV的高危因素。展开更多
文摘Specific nucleotide variations in the E2 DNA sequence were looked for in samples with an intact human papillomavirus (HPV) 16 episomal E2 DNA. Ninety two women, 76 with invasive cervical carcinoma and 16 with cervical intraepithelial neoplasia (CIN) were recruited. HPV DNA typing was performed by polymerase chain reaction (PCR) based restriction fragment length polymorphism (RFLP). Intact episomal E2 DNA of HPV 16 was detected by PCR. Important nucleotide variations in samples with amplifiable E2DNA were detected by RFLP. Nucleotide sequencing was performed on representative samples to confirm RFLP findings. A total of 89 (96.7% ) women were positive for HPV DNA. Of these, 56 (63% ) were positive for HPV 16, and of these, 38 (68% ) were positive for intact episomal HPV 16 E2 DNA while 18 (32% )- were negative. Samples with intact episomal HPV 16 E2DNA sequences were grouped into four different digestion profiles I to IV based on RFLP patterns. Digestion patterns revealed absence of any sequence variations in samples with digestion profile I and presence of a 2983 A- G variation in those with profile II. Samples with digestion profiles III and IV revealed three variations in the hinge region (3516 C- A, 3538 A- C, 3566 T- G) and two in the DNA binding domain (3684 C- A, 3694 T- A) of the E2 sequence. Sequencing performed on representative samples confirmed RFLP findings. PCR RFLP helped in the identification of important HPV 16 E2 sequence variations, circumventing the need for sequencing. The presence of the nucleotide variations in positions that could alter the biological and immunological functions of the E2 protein combined with its increased occurrence in this study bring out the importance of these variations.
