目的:血管生成是肿瘤生长和转移的关键介质,CDC25B在包括三阴性乳腺癌(Triple-Negative Breast Cancer,TNBC)等恶性肿瘤作为肿瘤癌基因,但其对血管生成的生物学作用知之甚少,本研究旨在探讨CDC25B在TNBC血管生成中的确切功能和作用机制...目的:血管生成是肿瘤生长和转移的关键介质,CDC25B在包括三阴性乳腺癌(Triple-Negative Breast Cancer,TNBC)等恶性肿瘤作为肿瘤癌基因,但其对血管生成的生物学作用知之甚少,本研究旨在探讨CDC25B在TNBC血管生成中的确切功能和作用机制。方法:生信数据库分析CDC25B及其上游调控分子miR-141-3p在TNBC肿瘤组织中的表达,采用qRT-PCR分析CDC25B和miR-141-3p在TNBC细胞系中的表达。利用CCK-8和血管形成实验分析HUVEC细胞的增殖和血管形成能力,并通过Western blot检测VEGFA、VEGFR-2和VEGFR-3蛋白的表达。双荧光素酶报告实验被用于探索CDC25B和miR-141-3p之间的特异性相互作用。结果:本研究发现CDC25B在TNBC中表达上调,其高表达可以激活VEGF信号通路,沉默CDC25B后显著抑制了HUVEC细胞的增殖和血管生成,并降低了VEGFA、VEGFR-2和VEGFR-3蛋白的表达。此外,miR-141-3p在TNBC中表达下调,可以靶向抑制CDC25B的表达。过表达CDC25B可以逆转miR-141-3p过表达对HUVEC细胞增殖和血管生成的抑制作用。结论:miR-141-3p靶向CDC25B抑制VEGF通路抑制TNBC血管生成,为miR-141-3p/CDC25B/VEGF通路可能作为TNBC抗血管生成治疗的新选择提供理论依据。展开更多
Enhanced formation of HCFC141b hydrate was studied with HCFC141b emulsion before the hydrate formation. Tergitol TMN6 was selected as emulgator, HCFC141b and water were introduced into an airtight emulsifier at the mo...Enhanced formation of HCFC141b hydrate was studied with HCFC141b emulsion before the hydrate formation. Tergitol TMN6 was selected as emulgator, HCFC141b and water were introduced into an airtight emulsifier at the molecular ratio of 17:1 and emulsified at the temperature of 14℃ under the stirring speed of 4000～6400 r/min. And HCFC141b hydrate formed and decomposed with the change of programmed temperature. The experimental results showed that with the new method HCFC141b hydrate began to form in the time of 30 min (in glycol-water bath) or 100 min (in air bath) even in static state, and under the same condition with conventional method (non-emulsion) the hydrate could not form, at least it difficultly formed. And in dynamic state HCFC141b emulsion formed hydrate in 20 min earlier than the mixed liquid did, liquid from hydrate decomposition was emulsified after brief stirring, and formed hydrate again more easily than the brand-new emulsion.展开更多
In order to design a kind of heat exchanger suitable to the indirect-touched gas hydrate cool storage vessel, a visual observation of HCFC141b gas hydrate formation/decomposition process was presented through a self-d...In order to design a kind of heat exchanger suitable to the indirect-touched gas hydrate cool storage vessel, a visual observation of HCFC141b gas hydrate formation/decomposition process was presented through a self-designed small-scale visualization apparatus of gas hydrate cool storage. Based on the shooted photos and recorded temperatures, the formation/decomposition process of HCFC141b are described, some characteristics are concluded, and some suggestions of designing heat exchanger are indicated according to the specific characteristics of HCFC141b gas hydrate formation/decomposition process.展开更多
文摘目的:血管生成是肿瘤生长和转移的关键介质,CDC25B在包括三阴性乳腺癌(Triple-Negative Breast Cancer,TNBC)等恶性肿瘤作为肿瘤癌基因,但其对血管生成的生物学作用知之甚少,本研究旨在探讨CDC25B在TNBC血管生成中的确切功能和作用机制。方法:生信数据库分析CDC25B及其上游调控分子miR-141-3p在TNBC肿瘤组织中的表达,采用qRT-PCR分析CDC25B和miR-141-3p在TNBC细胞系中的表达。利用CCK-8和血管形成实验分析HUVEC细胞的增殖和血管形成能力,并通过Western blot检测VEGFA、VEGFR-2和VEGFR-3蛋白的表达。双荧光素酶报告实验被用于探索CDC25B和miR-141-3p之间的特异性相互作用。结果:本研究发现CDC25B在TNBC中表达上调,其高表达可以激活VEGF信号通路,沉默CDC25B后显著抑制了HUVEC细胞的增殖和血管生成,并降低了VEGFA、VEGFR-2和VEGFR-3蛋白的表达。此外,miR-141-3p在TNBC中表达下调,可以靶向抑制CDC25B的表达。过表达CDC25B可以逆转miR-141-3p过表达对HUVEC细胞增殖和血管生成的抑制作用。结论:miR-141-3p靶向CDC25B抑制VEGF通路抑制TNBC血管生成,为miR-141-3p/CDC25B/VEGF通路可能作为TNBC抗血管生成治疗的新选择提供理论依据。
基金Supported by Guangzhou Center for Gas Hydrate Research, Chinese Academy of Sciences (No.CASHYD0807s6)
文摘Enhanced formation of HCFC141b hydrate was studied with HCFC141b emulsion before the hydrate formation. Tergitol TMN6 was selected as emulgator, HCFC141b and water were introduced into an airtight emulsifier at the molecular ratio of 17:1 and emulsified at the temperature of 14℃ under the stirring speed of 4000～6400 r/min. And HCFC141b hydrate formed and decomposed with the change of programmed temperature. The experimental results showed that with the new method HCFC141b hydrate began to form in the time of 30 min (in glycol-water bath) or 100 min (in air bath) even in static state, and under the same condition with conventional method (non-emulsion) the hydrate could not form, at least it difficultly formed. And in dynamic state HCFC141b emulsion formed hydrate in 20 min earlier than the mixed liquid did, liquid from hydrate decomposition was emulsified after brief stirring, and formed hydrate again more easily than the brand-new emulsion.
基金supported by the National Natural Science Foundation of China (No. 50176051, No. 59836230)the Satate Key Development Program for Basic Research of China (No. 2000026306).
文摘In order to design a kind of heat exchanger suitable to the indirect-touched gas hydrate cool storage vessel, a visual observation of HCFC141b gas hydrate formation/decomposition process was presented through a self-designed small-scale visualization apparatus of gas hydrate cool storage. Based on the shooted photos and recorded temperatures, the formation/decomposition process of HCFC141b are described, some characteristics are concluded, and some suggestions of designing heat exchanger are indicated according to the specific characteristics of HCFC141b gas hydrate formation/decomposition process.
