Introduction: The prevention of transmission of infections transmitted by blood transfusion depends on the correct selection of donors and the performance of adequate serological tests for the detection of pathogens, ...Introduction: The prevention of transmission of infections transmitted by blood transfusion depends on the correct selection of donors and the performance of adequate serological tests for the detection of pathogens, in particular viral hepatitis B and C viruses. The main objective of our study was to evaluate the seroprevalences of viral markers B and C in blood donors in the Agadir region. The secondary objectives were to evaluate these prevalences according to the socio-demographic characteristics of the donors and to evaluate the seroprevalence of other markers, in particular the human immunodeficiency virus. Methods: A retrospective analysis of files from the blood donor registers of the Agadir regional transfusion center, covering the period from 1 January to 31 December 2020, was conducted. Results: The study included 13,092 donors, the mean age was 34 ± 11, the M/F sex ratio was 2.6. The overall seroprevalences of HBsAg, anti-HCV, anti-HIV were respectively: 1.3%, 0.2% and 0.3% respectively. For HBsAg, the prevalence was higher in men (p = 0.007). The prevalence of anti-HCV was higher in rural areas than in urban areas (p Conclusion: The low prevalences of viral markers B and C in our study compared to those recorded in the general Moroccan population reflect the effectiveness of preventive measures with regard to donor selection.展开更多
目的了解无偿献血人群丙型肝炎病毒(HCV)感染者中隐匿性乙肝感染(OBI)率,为进一步阐明HCV/OBI的作用机制提供科学依据。方法采用盖立复PROCLEIX TIGRIS、罗氏cobas s 201和罗氏E601等仪器对2011年1月—2015年7月广州无偿献血者中的933(...目的了解无偿献血人群丙型肝炎病毒(HCV)感染者中隐匿性乙肝感染(OBI)率,为进一步阐明HCV/OBI的作用机制提供科学依据。方法采用盖立复PROCLEIX TIGRIS、罗氏cobas s 201和罗氏E601等仪器对2011年1月—2015年7月广州无偿献血者中的933(人)份HCV感染者标本做HBV DNA和抗-HBc检测,比较HCV单纯感染者和HCV/OBI共感染者之间性别、年龄、民族和转氨酶的差异;巢式PCR扩增HCV/OBI共感染者HCV C区和S区,并构建进化树和基因分型,荧光定量PCR检测HCV和HBV的病毒载量;另外,随机选取同期本地HCV 6a基因型的44名单纯HCV感染者和16名HCV/HBV共感染者最为对照组,通过巢式PCR扩增各组HCV C区,并对3组对象的HCV核心蛋白氨基酸序列做比对分析。结果本组HCV感染的献血者标本中,有6(人)份HBV DNA和抗-HBc阳性,亦即OBI感染率为6.43‰(6/933);该6份HCV/OBI共感染标本的HCV基因型中6a占83.3%(5/6)、1b占16.7%(1/6),HCV病毒载量(log_(10)IU/mL)为3.8—5.7(5.0±0.69)。HCV单纯感染者和HCV/OBI共感染者的性别、年龄、民族、籍贯和ALT均无明显差异(P>0.05)。HCV单纯感染者、HCV/HBV共感染者和HCV/OBI共感染者HCV核心蛋白序列氨基酸变异数分别为0.8±1.3 vs 0.7±1.5 vs 0.4±0.5(P>0.05),没有在HCV/OBI共感染者中发现一致性的结构或突变规律。结论广州地区无偿献血人群HCV感染者中的OBI阳性率较高。展开更多
文摘Introduction: The prevention of transmission of infections transmitted by blood transfusion depends on the correct selection of donors and the performance of adequate serological tests for the detection of pathogens, in particular viral hepatitis B and C viruses. The main objective of our study was to evaluate the seroprevalences of viral markers B and C in blood donors in the Agadir region. The secondary objectives were to evaluate these prevalences according to the socio-demographic characteristics of the donors and to evaluate the seroprevalence of other markers, in particular the human immunodeficiency virus. Methods: A retrospective analysis of files from the blood donor registers of the Agadir regional transfusion center, covering the period from 1 January to 31 December 2020, was conducted. Results: The study included 13,092 donors, the mean age was 34 ± 11, the M/F sex ratio was 2.6. The overall seroprevalences of HBsAg, anti-HCV, anti-HIV were respectively: 1.3%, 0.2% and 0.3% respectively. For HBsAg, the prevalence was higher in men (p = 0.007). The prevalence of anti-HCV was higher in rural areas than in urban areas (p Conclusion: The low prevalences of viral markers B and C in our study compared to those recorded in the general Moroccan population reflect the effectiveness of preventive measures with regard to donor selection.
文摘目的了解无偿献血人群丙型肝炎病毒(HCV)感染者中隐匿性乙肝感染(OBI)率,为进一步阐明HCV/OBI的作用机制提供科学依据。方法采用盖立复PROCLEIX TIGRIS、罗氏cobas s 201和罗氏E601等仪器对2011年1月—2015年7月广州无偿献血者中的933(人)份HCV感染者标本做HBV DNA和抗-HBc检测,比较HCV单纯感染者和HCV/OBI共感染者之间性别、年龄、民族和转氨酶的差异;巢式PCR扩增HCV/OBI共感染者HCV C区和S区,并构建进化树和基因分型,荧光定量PCR检测HCV和HBV的病毒载量;另外,随机选取同期本地HCV 6a基因型的44名单纯HCV感染者和16名HCV/HBV共感染者最为对照组,通过巢式PCR扩增各组HCV C区,并对3组对象的HCV核心蛋白氨基酸序列做比对分析。结果本组HCV感染的献血者标本中,有6(人)份HBV DNA和抗-HBc阳性,亦即OBI感染率为6.43‰(6/933);该6份HCV/OBI共感染标本的HCV基因型中6a占83.3%(5/6)、1b占16.7%(1/6),HCV病毒载量(log_(10)IU/mL)为3.8—5.7(5.0±0.69)。HCV单纯感染者和HCV/OBI共感染者的性别、年龄、民族、籍贯和ALT均无明显差异(P>0.05)。HCV单纯感染者、HCV/HBV共感染者和HCV/OBI共感染者HCV核心蛋白序列氨基酸变异数分别为0.8±1.3 vs 0.7±1.5 vs 0.4±0.5(P>0.05),没有在HCV/OBI共感染者中发现一致性的结构或突变规律。结论广州地区无偿献血人群HCV感染者中的OBI阳性率较高。
