Using horseradish peroxidase (HRP)-HDL3, instead of the classical 125 I-HDL3, an enzyme linked assay for measuring HDL recptor on cultured human arterial smooth muscle cells (SMC )was developed. Horseradish peroxidase...Using horseradish peroxidase (HRP)-HDL3, instead of the classical 125 I-HDL3, an enzyme linked assay for measuring HDL recptor on cultured human arterial smooth muscle cells (SMC )was developed. Horseradish peroxidase labeled HDI3, was prepared by periodate method.Human arterial SMC were fixed on 96-wells plastic plates by glutaraldehyde mehtod. The results showed that Scatchard plot of high affinity binding of high affinity binding of HRP-HDL3 to SMC was significantly linear,r=—0.987. The Kd and Bmax were 13.12±0.8μg/ml and 106±9.3 ng/mg cell protein, respectively. HDL3 competed effectively for binding of HRP-HDL3 to SMC. On the contrary,LDL and albumin did not compete with HRP-HDL binding. The binding was Ca2+ independent and not sensitive to trypsin. These results indieated that human arterial smooth muscle cells possess specific binding sites for HDL with high affinity.展开更多
目的 研究小鼠肝细胞膜载脂蛋白 (apo) C 受体与 apo C 、C 、C 及 E的结合特性。方法 用受体的放射性配基结合分析法 (RBA )观察血浆脂蛋白 (VL DL、L DL及 HDL )以及纯化的 apo C 、C 、C 及 E与小鼠肝细胞膜 apo C 受体结合的活性...目的 研究小鼠肝细胞膜载脂蛋白 (apo) C 受体与 apo C 、C 、C 及 E的结合特性。方法 用受体的放射性配基结合分析法 (RBA )观察血浆脂蛋白 (VL DL、L DL及 HDL )以及纯化的 apo C 、C 、C 及 E与小鼠肝细胞膜 apo C 受体结合的活性。结果 apo C 受体与 1 2 5I- apo C 的结合能被非标记 apo C 、C 、C 、E及 VL DL抑制 ,其中 apo C 、C 、C 及 VL DL抑制曲线相似 ,以 apo C 的抑制作用最强 ,apo C 次之 ,apo C 最弱 ,apo E与 apo C 受体亦有一定结合 ;而含 apo B10 0的 L DL以及含 apo A 及 apo A 的 HDL和人血清白蛋白则对结合无影响。结论 小鼠肝细胞膜上存在 apo C 受体 ,除能特异结合 apo C 及 VL DL外 ,亦能在一定程度上与apo C 、C 及 E结合。展开更多
文摘Using horseradish peroxidase (HRP)-HDL3, instead of the classical 125 I-HDL3, an enzyme linked assay for measuring HDL recptor on cultured human arterial smooth muscle cells (SMC )was developed. Horseradish peroxidase labeled HDI3, was prepared by periodate method.Human arterial SMC were fixed on 96-wells plastic plates by glutaraldehyde mehtod. The results showed that Scatchard plot of high affinity binding of high affinity binding of HRP-HDL3 to SMC was significantly linear,r=—0.987. The Kd and Bmax were 13.12±0.8μg/ml and 106±9.3 ng/mg cell protein, respectively. HDL3 competed effectively for binding of HRP-HDL3 to SMC. On the contrary,LDL and albumin did not compete with HRP-HDL binding. The binding was Ca2+ independent and not sensitive to trypsin. These results indieated that human arterial smooth muscle cells possess specific binding sites for HDL with high affinity.
文摘目的 研究小鼠肝细胞膜载脂蛋白 (apo) C 受体与 apo C 、C 、C 及 E的结合特性。方法 用受体的放射性配基结合分析法 (RBA )观察血浆脂蛋白 (VL DL、L DL及 HDL )以及纯化的 apo C 、C 、C 及 E与小鼠肝细胞膜 apo C 受体结合的活性。结果 apo C 受体与 1 2 5I- apo C 的结合能被非标记 apo C 、C 、C 、E及 VL DL抑制 ,其中 apo C 、C 、C 及 VL DL抑制曲线相似 ,以 apo C 的抑制作用最强 ,apo C 次之 ,apo C 最弱 ,apo E与 apo C 受体亦有一定结合 ;而含 apo B10 0的 L DL以及含 apo A 及 apo A 的 HDL和人血清白蛋白则对结合无影响。结论 小鼠肝细胞膜上存在 apo C 受体 ,除能特异结合 apo C 及 VL DL外 ,亦能在一定程度上与apo C 、C 及 E结合。