目的:探讨改进血清和试剂对高密度脂蛋白-胆固醇(high density lipoprotein cholesterol,HDL-ch)含量的测定结果是否有影响。方法:用磷钨酸-镁沉淀法测定HDL-ch的含量。结果:使用两种不同试剂测定的HDL-ch值差异无统计学意义(P>0.05...目的:探讨改进血清和试剂对高密度脂蛋白-胆固醇(high density lipoprotein cholesterol,HDL-ch)含量的测定结果是否有影响。方法:用磷钨酸-镁沉淀法测定HDL-ch的含量。结果:使用两种不同试剂测定的HDL-ch值差异无统计学意义(P>0.05)。结论:通过血清和试剂的改进,对实验结果无影响,但对学生和教师的安全性得到了提高。展开更多
Objective: To observe the effect of herb-medicine-cake-separated moxibustion on serum lipoprotein in hyperlipemia rabbits. Methods: 55 New-Zealand rabbits were randomly divided into control group (n=13), model group (...Objective: To observe the effect of herb-medicine-cake-separated moxibustion on serum lipoprotein in hyperlipemia rabbits. Methods: 55 New-Zealand rabbits were randomly divided into control group (n=13), model group (n=14), direct moxibustion group (n=14) and herb-medicine-cake-separated moxibustion (indirect moxibustion) group (n=14). Hyperlipemia model was established by feeding the animals with specialized forage (15% vitellus powder, 5% lard, 0.5% cholesterol and common forage) for 6 weeks. Moxibustion was applied to “Juque”(CV 14), “Tianshu”(ST 25), “Fenglong”(ST 40), etc., 4 moxa-cones for every acupoint, once daily and continuously for 40 days. Serum high density lipoprotein-cholesterol (HDL-Ch), low density lipoprotein-cholesterol (LDL-Ch) and total cholesterol (TCh) contents were assayed with colorimetric method. Results: Compared with control group, serum LDL-Ch content, HDL-Ch/LDL-Ch and HDL-Ch/TCh of model group were significantly higher (P<0.05～0.01), while compared with model group, LDL-Ch contents of two moxibustion groups were strikingly lower (P<0.01). No significant differences were found between two moxibustion groups in all the 4 indexes. Conclusion: Both direct and indirect moxibustion can effectively lower serum LDL-Ch, raise HDL-Ch, HDL-Ch/LDL-Ch and HDL-Ch/TCh, and regulate lipoprotein metabolism in hyperlipemia rabbits.展开更多
The differences of serum lipid and lipoprotein (LP,profiles of animals susceptible (rabbit)and nonsusceptible (Beijing duck) to atherosclerosis as well as the distribution of apolipo-protein (apo) A-I and its cataboli...The differences of serum lipid and lipoprotein (LP,profiles of animals susceptible (rabbit)and nonsusceptible (Beijing duck) to atherosclerosis as well as the distribution of apolipo-protein (apo) A-I and its catabolism in vivo were studied.Eight items,i.e.total cholesterol(TC),high density lipoprotein (HDL) cholesterol,triglyceride,percentage of a-LP and β-LP,β-LP concentration,lecithin cholesterol acyl transferase (LCAT),and agarose and polyacryla-mide gel electrophoresis of both species were assayed before and after feeding a high fat,high cholesterol diet.Results indicate that the exogenous cholesterol consumed by Beijingducks was carried and transported by HDL,while that in rabbits was transported by lowdensity lipoprotein (LDL).The biological half lives of apo A-I in serum and in HDL were41.11±2.4 and 42.8±1.7 h respectively.and its distribution in different organs was in theorder of liver】kidney】spleen】lung】heart】intestine】muscles】aorta.These resultsshow that the liver is the major organ for metabolizing HDL apo A-I.and the kidneyis also important.The results also imply that in Beijing ducks the cholesterol carried byHDL may be catabolized through apo A-I receptors in the liver and kidney.The differencesin cholesterol binding and in lipoprotein and apolipoprotein metabolism of the two speciesprovide important clues for the elucidation of the pathogenesis of atherosclerosis.展开更多
Objective: To observe effects of herbal cake-separated moxibustion on blood lipids, including total cholesterol (TCh), triglyceride (TG), high density lipoprotein-Ch (HDL-Ch), low density lipoprotein-Ch (LDL-Ch), apol...Objective: To observe effects of herbal cake-separated moxibustion on blood lipids, including total cholesterol (TCh), triglyceride (TG), high density lipoprotein-Ch (HDL-Ch), low density lipoprotein-Ch (LDL-Ch), apolipoprotein A (Apo A), apolipoprotein B (Apo B), and plasma thromboxane B2 (TXB2) and 6-keto-prostaglandin F 1α (6-keto-PGF 1α) contents and analyse its mechanism. Methods: The hyperlipemia rabbit model was established by breeding of high fat forage and injection of bovine serum albumin. They were treated respectively by direct moxibustion and herbal cake-separated moxibustion at Juque (巨阙 CV 14), Tianshu (天枢 ST 25), Fenglong (丰隆 ST 40), etc., once daily, continuously for 40 days. Blood TCh and TG contents were detected with enzymatic method, LDL-Ch and HDL-Ch with colorimetric method, Apo A and Apo B with electrophoretic method, and TXB2 and 6-keto-PGF 1α with radioimmunoassay. Results: Both the herbal cake-separated moxibustion and direct moxibustion could effectively decrease TCh, TG, LDL-Ch, Apo B and TXB2 contents and TXB2/6-keto-PGF 1α, and increase HDL-CH and 6-keto-PGF 1α contents in the rabbit of hyperlipemia. Conclusion: 6-keto-PGF 1α and TXB2 are possibly involved in the mechanism of herbal cake-separated moxibustion decreasing blood lipids.展开更多
文摘目的:探讨改进血清和试剂对高密度脂蛋白-胆固醇(high density lipoprotein cholesterol,HDL-ch)含量的测定结果是否有影响。方法:用磷钨酸-镁沉淀法测定HDL-ch的含量。结果:使用两种不同试剂测定的HDL-ch值差异无统计学意义(P>0.05)。结论:通过血清和试剂的改进,对实验结果无影响,但对学生和教师的安全性得到了提高。
文摘Objective: To observe the effect of herb-medicine-cake-separated moxibustion on serum lipoprotein in hyperlipemia rabbits. Methods: 55 New-Zealand rabbits were randomly divided into control group (n=13), model group (n=14), direct moxibustion group (n=14) and herb-medicine-cake-separated moxibustion (indirect moxibustion) group (n=14). Hyperlipemia model was established by feeding the animals with specialized forage (15% vitellus powder, 5% lard, 0.5% cholesterol and common forage) for 6 weeks. Moxibustion was applied to “Juque”(CV 14), “Tianshu”(ST 25), “Fenglong”(ST 40), etc., 4 moxa-cones for every acupoint, once daily and continuously for 40 days. Serum high density lipoprotein-cholesterol (HDL-Ch), low density lipoprotein-cholesterol (LDL-Ch) and total cholesterol (TCh) contents were assayed with colorimetric method. Results: Compared with control group, serum LDL-Ch content, HDL-Ch/LDL-Ch and HDL-Ch/TCh of model group were significantly higher (P<0.05～0.01), while compared with model group, LDL-Ch contents of two moxibustion groups were strikingly lower (P<0.01). No significant differences were found between two moxibustion groups in all the 4 indexes. Conclusion: Both direct and indirect moxibustion can effectively lower serum LDL-Ch, raise HDL-Ch, HDL-Ch/LDL-Ch and HDL-Ch/TCh, and regulate lipoprotein metabolism in hyperlipemia rabbits.
基金This investigation was supported by grants from the National Committee of Science and Technology and from the Chinese Ministry of Public Healthand was reported in the International Symposium on Cardiovascular Medicine and Surgery.1987,Beijing.
文摘The differences of serum lipid and lipoprotein (LP,profiles of animals susceptible (rabbit)and nonsusceptible (Beijing duck) to atherosclerosis as well as the distribution of apolipo-protein (apo) A-I and its catabolism in vivo were studied.Eight items,i.e.total cholesterol(TC),high density lipoprotein (HDL) cholesterol,triglyceride,percentage of a-LP and β-LP,β-LP concentration,lecithin cholesterol acyl transferase (LCAT),and agarose and polyacryla-mide gel electrophoresis of both species were assayed before and after feeding a high fat,high cholesterol diet.Results indicate that the exogenous cholesterol consumed by Beijingducks was carried and transported by HDL,while that in rabbits was transported by lowdensity lipoprotein (LDL).The biological half lives of apo A-I in serum and in HDL were41.11±2.4 and 42.8±1.7 h respectively.and its distribution in different organs was in theorder of liver】kidney】spleen】lung】heart】intestine】muscles】aorta.These resultsshow that the liver is the major organ for metabolizing HDL apo A-I.and the kidneyis also important.The results also imply that in Beijing ducks the cholesterol carried byHDL may be catabolized through apo A-I receptors in the liver and kidney.The differencesin cholesterol binding and in lipoprotein and apolipoprotein metabolism of the two speciesprovide important clues for the elucidation of the pathogenesis of atherosclerosis.
文摘Objective: To observe effects of herbal cake-separated moxibustion on blood lipids, including total cholesterol (TCh), triglyceride (TG), high density lipoprotein-Ch (HDL-Ch), low density lipoprotein-Ch (LDL-Ch), apolipoprotein A (Apo A), apolipoprotein B (Apo B), and plasma thromboxane B2 (TXB2) and 6-keto-prostaglandin F 1α (6-keto-PGF 1α) contents and analyse its mechanism. Methods: The hyperlipemia rabbit model was established by breeding of high fat forage and injection of bovine serum albumin. They were treated respectively by direct moxibustion and herbal cake-separated moxibustion at Juque (巨阙 CV 14), Tianshu (天枢 ST 25), Fenglong (丰隆 ST 40), etc., once daily, continuously for 40 days. Blood TCh and TG contents were detected with enzymatic method, LDL-Ch and HDL-Ch with colorimetric method, Apo A and Apo B with electrophoretic method, and TXB2 and 6-keto-PGF 1α with radioimmunoassay. Results: Both the herbal cake-separated moxibustion and direct moxibustion could effectively decrease TCh, TG, LDL-Ch, Apo B and TXB2 contents and TXB2/6-keto-PGF 1α, and increase HDL-CH and 6-keto-PGF 1α contents in the rabbit of hyperlipemia. Conclusion: 6-keto-PGF 1α and TXB2 are possibly involved in the mechanism of herbal cake-separated moxibustion decreasing blood lipids.
