To investigate the biological and immunological characteristics of the Nanjing local strains of HHV-7,four strains of herpesvirus were isolated from saliva specimens of one healthy individual and three children suffer...To investigate the biological and immunological characteristics of the Nanjing local strains of HHV-7,four strains of herpesvirus were isolated from saliva specimens of one healthy individual and three children suffering from a kidney disease in Nanjing.The viruses were identified by transmission electron microscopy (EM),indirect immunofluorescence assay(IFA) with a specific monoclonal antibody;nested polymerase chain reaction,restriction mapping and DNA sequencing.The virus-infected cells showed the typical cytophathic effect(CPE) under microscopy and could be detected by IFA with the human herpesvirus-7(HHV-7) specific antibody.Under EM,herpesvirus-like and virions capsids could be found in their cytoplasm or nucleoplasm. HHV-7 DNA fragments amplified from infected cells by nested PCR were confirmed by restriction mapping and DNA sequencing.Similarly to DC strain,an known HHV-7 strain used in the present study as the positive control,the virus could be inactivated by ultraviolet irradiation for 10 min,heated at 45℃ for 30 min,pH<5 or>9 at 4℃ for 2 h and ether or chloroform for 10 h.The virus induced the production of TNF-α,IL-10 and IL-12p70 while inhibited IFN-γ secretion,increased the percentage of CD2^+ cells while decreased that of CD4^+ or CD45RA^+ cells.The results indicate that the viruses isolated in Nanjing are HHV-7,which has similar biological characteristic to the known HHV-7 strain,DC.Infection with HHV-7 in vitro could affect immune function of lymphocytes by disturbing cytokine production and CD antigen expression.Cellular & Molecular Immunology.2004;1(5):367-372.展开更多
Background:Human herpesvirus(HHV)-7 is usually associated with febrile seizures.Later onset and higher frequency of seizures are characteristic of pediatric HHV-7,compared with HHV-6 infection.The HHV-7-related severe...Background:Human herpesvirus(HHV)-7 is usually associated with febrile seizures.Later onset and higher frequency of seizures are characteristic of pediatric HHV-7,compared with HHV-6 infection.The HHV-7-related severe neurological disorders are predominantly observed in immunocompromised individuals.Reports of healthy individuals with HHV-7 infection and diverse neurological disorders are limited.Patient Description:We present a case of HHV-7-related epilepsy in an immunocompetent 11-year old boy and extensive infectious and autoimmune testing positive only for HHV-7 in the cerebrospinal fluid.The patient made a good recovery after treatment with intravenous immunoglobulin and methylprednisolone.Discussion:This is the first reported case of epilepsy associated with HHV-7 in a previously healthy individual.It also demonstrates that intravenous immunoglobulin and steroids may be used in the course of this disorder and may be beneficial for recovery.展开更多
Background: Lichen planus (LP) is a common inflammatory skin disease of unknown aetiology. Viral causes have been suggested. Objectives: To find candidate viruses associated with LP.Methods: Lesional and nonlesional s...Background: Lichen planus (LP) is a common inflammatory skin disease of unknown aetiology. Viral causes have been suggested. Objectives: To find candidate viruses associated with LP.Methods: Lesional and nonlesional skin samples,peripheral blood mononuclear cells and serum were obtained from patients with LP. Ultrastructural, viral DNA, immunohistochemical and serological analyses were performed, and comparisons were made with psoriatic and normal skin. Results: Electron microscopy revealed typical 120-200-nm enveloped particles with a 100-nm nucleus resembling human herpesvirus (HHV) virions both in dermis and in epidermis of lesional LP tissue. HHV-7 DNA was found in 11 of 18 lesional LP samples, as opposed to only one of 11 nonlesional LP samples (P =0.06), two of 11 lesional psoriasis samples (P = 0.05) and none of four normal skin samples. No relation was found between LP skin and DNA of other known HHVs (HHV-1,-6 and 8). With immunohistochemistry, significantly more HHV-7+cells were found in lesional LP epidermis than in normal epidermis. Lesional LP dermis contained significantly more HHV-7+cells than nonlesional LP, psoriatic or normal dermis. Moreover, LP skin contained overwhelmingly and consistently more plasmacytoid dendritic cells (upregulated in virally induced conditions) than nonlesional LP samples. Conclusions: We conclude that HHV-7 replicates in LP lesions, but not in psoriasis, another inflammatory skin condition. HHV-7 is possibly involved in the pathogenesis of LP. These preliminary data make further research on this topic of interest.展开更多
基金This work was supported by grants from Natural Science Foundation of Nation(NO.30371345)Jiangsu Province(NO.BK970S1 and BK2001115).
文摘To investigate the biological and immunological characteristics of the Nanjing local strains of HHV-7,four strains of herpesvirus were isolated from saliva specimens of one healthy individual and three children suffering from a kidney disease in Nanjing.The viruses were identified by transmission electron microscopy (EM),indirect immunofluorescence assay(IFA) with a specific monoclonal antibody;nested polymerase chain reaction,restriction mapping and DNA sequencing.The virus-infected cells showed the typical cytophathic effect(CPE) under microscopy and could be detected by IFA with the human herpesvirus-7(HHV-7) specific antibody.Under EM,herpesvirus-like and virions capsids could be found in their cytoplasm or nucleoplasm. HHV-7 DNA fragments amplified from infected cells by nested PCR were confirmed by restriction mapping and DNA sequencing.Similarly to DC strain,an known HHV-7 strain used in the present study as the positive control,the virus could be inactivated by ultraviolet irradiation for 10 min,heated at 45℃ for 30 min,pH<5 or>9 at 4℃ for 2 h and ether or chloroform for 10 h.The virus induced the production of TNF-α,IL-10 and IL-12p70 while inhibited IFN-γ secretion,increased the percentage of CD2^+ cells while decreased that of CD4^+ or CD45RA^+ cells.The results indicate that the viruses isolated in Nanjing are HHV-7,which has similar biological characteristic to the known HHV-7 strain,DC.Infection with HHV-7 in vitro could affect immune function of lymphocytes by disturbing cytokine production and CD antigen expression.Cellular & Molecular Immunology.2004;1(5):367-372.
文摘Background:Human herpesvirus(HHV)-7 is usually associated with febrile seizures.Later onset and higher frequency of seizures are characteristic of pediatric HHV-7,compared with HHV-6 infection.The HHV-7-related severe neurological disorders are predominantly observed in immunocompromised individuals.Reports of healthy individuals with HHV-7 infection and diverse neurological disorders are limited.Patient Description:We present a case of HHV-7-related epilepsy in an immunocompetent 11-year old boy and extensive infectious and autoimmune testing positive only for HHV-7 in the cerebrospinal fluid.The patient made a good recovery after treatment with intravenous immunoglobulin and methylprednisolone.Discussion:This is the first reported case of epilepsy associated with HHV-7 in a previously healthy individual.It also demonstrates that intravenous immunoglobulin and steroids may be used in the course of this disorder and may be beneficial for recovery.
文摘Background: Lichen planus (LP) is a common inflammatory skin disease of unknown aetiology. Viral causes have been suggested. Objectives: To find candidate viruses associated with LP.Methods: Lesional and nonlesional skin samples,peripheral blood mononuclear cells and serum were obtained from patients with LP. Ultrastructural, viral DNA, immunohistochemical and serological analyses were performed, and comparisons were made with psoriatic and normal skin. Results: Electron microscopy revealed typical 120-200-nm enveloped particles with a 100-nm nucleus resembling human herpesvirus (HHV) virions both in dermis and in epidermis of lesional LP tissue. HHV-7 DNA was found in 11 of 18 lesional LP samples, as opposed to only one of 11 nonlesional LP samples (P =0.06), two of 11 lesional psoriasis samples (P = 0.05) and none of four normal skin samples. No relation was found between LP skin and DNA of other known HHVs (HHV-1,-6 and 8). With immunohistochemistry, significantly more HHV-7+cells were found in lesional LP epidermis than in normal epidermis. Lesional LP dermis contained significantly more HHV-7+cells than nonlesional LP, psoriatic or normal dermis. Moreover, LP skin contained overwhelmingly and consistently more plasmacytoid dendritic cells (upregulated in virally induced conditions) than nonlesional LP samples. Conclusions: We conclude that HHV-7 replicates in LP lesions, but not in psoriasis, another inflammatory skin condition. HHV-7 is possibly involved in the pathogenesis of LP. These preliminary data make further research on this topic of interest.
