This letter presents an autofocus (AF) method to position a high-magnification microscope lens that automatically captures hundreds of images from a single moving slide. These images are taken by a mobile clinic uni...This letter presents an autofocus (AF) method to position a high-magnification microscope lens that automatically captures hundreds of images from a single moving slide. These images are taken by a mobile clinic unit in a rural location, and are later automatically processed and revised by a remote specialist. This process requires high focus precision to enable image processing techniques to achieve proper results. Low focusing times are also required for the system to be operative. We propose a novel method that combines two focus measures with an adapted searching scheme to cope with both constraints.展开更多
Over the last years, major improvements in the field of male infertility diagnosis have been achieved. The aim of this study was to determine the diagnostic usefulness of sperm DNA integrity and sperm vacuolisation fo...Over the last years, major improvements in the field of male infertility diagnosis have been achieved. The aim of this study was to determine the diagnostic usefulness of sperm DNA integrity and sperm vacuolisation for predicting outcome in infertile couples undergoing in vitroferti lisation (IVF) and intracytoplasmic sperm injection (ICSI) treatments. A cohort study from 152 infertile couples undergoing sperm DNA fragmentation and high-magnification tests prior to an assisted reproduction treatment was designed. We found that the most predictive cutoff for pregnancy was 25.5% of DNA fragmentation with a negative predictive value of 72.7% (P=0.02). For the degree of vacuolisation, the best predictor of pregnancy was 73.5% of vacuolated sperm grades Ⅲ + Ⅳ with a negative predictive value of 39.4% (P=0.09), which was not statistically significant. In conclusion, sperm DNA fragmentation greater than 25.5% could be associated with higher probability of failure IVF treatment. Regarding the results of the sperm analysis at high magnification, they do not allow us to predict whether or not oatients will become pregnant.展开更多
基金supported by the CONACYT/204212the DGEST of the Mexican Government under the PROMEP/107.5/10/5417
文摘This letter presents an autofocus (AF) method to position a high-magnification microscope lens that automatically captures hundreds of images from a single moving slide. These images are taken by a mobile clinic unit in a rural location, and are later automatically processed and revised by a remote specialist. This process requires high focus precision to enable image processing techniques to achieve proper results. Low focusing times are also required for the system to be operative. We propose a novel method that combines two focus measures with an adapted searching scheme to cope with both constraints.
文摘Over the last years, major improvements in the field of male infertility diagnosis have been achieved. The aim of this study was to determine the diagnostic usefulness of sperm DNA integrity and sperm vacuolisation for predicting outcome in infertile couples undergoing in vitroferti lisation (IVF) and intracytoplasmic sperm injection (ICSI) treatments. A cohort study from 152 infertile couples undergoing sperm DNA fragmentation and high-magnification tests prior to an assisted reproduction treatment was designed. We found that the most predictive cutoff for pregnancy was 25.5% of DNA fragmentation with a negative predictive value of 72.7% (P=0.02). For the degree of vacuolisation, the best predictor of pregnancy was 73.5% of vacuolated sperm grades Ⅲ + Ⅳ with a negative predictive value of 39.4% (P=0.09), which was not statistically significant. In conclusion, sperm DNA fragmentation greater than 25.5% could be associated with higher probability of failure IVF treatment. Regarding the results of the sperm analysis at high magnification, they do not allow us to predict whether or not oatients will become pregnant.