目的了解青岛市男男性行为者(men who have sex with men,MSM)新发HIV-1感染者的病毒基因分型特征,构建HIV-1分子传播网络,为精准干预提供依据.方法对2022年青岛市MSM新发HIV-1感染者进行问卷调查并采集血样,提取HIV-1病毒RNA,巢式PCR扩...目的了解青岛市男男性行为者(men who have sex with men,MSM)新发HIV-1感染者的病毒基因分型特征,构建HIV-1分子传播网络,为精准干预提供依据.方法对2022年青岛市MSM新发HIV-1感染者进行问卷调查并采集血样,提取HIV-1病毒RNA,巢式PCR扩增pol区基因片段并进行测序,确定基因分型,计算基因距离,使用MEGA v11软件、HIV-Trace等构建系统进化树与分子网络,使用SPSS 27.0进行入网影响因素分析.结果共调查158名MSM,采集152人血液样本,成功扩增135条pol区序列,共11种基因分型,主要基因分型为:CRF07_BC占42.23%(57/135),CRF01_AE占35.56%(48/135),CRF55_01B占9.63%(13/135);检出了较为特殊的复杂重组体CRF01_BC和二代重组体CRF0107.鉴定出13个传播簇,CRF07_BC形成最大的传播簇,包含43个节点,CRF01_AE包含15个节点,CRF55_01B包含11个节点.在0.03%的基因距离阈值下构建分子网络,入网率为51.11%(69/135).经χ^(2)检验与Fisher精确检验显示,HIV-1基因分型(χ^(2)=44.691,P<0.01)、是否独居(χ^(2)=4.972,P=0.026)、对艾滋病了解程度(P=0.005)进入分子网络的差异有统计学意义.检测出44条序列存在耐药突变位点,耐药突变率为32.59%(44/135),21例进入分子网络.在基因型为CRF55_01B的一个传播簇中,所有感染者均对NNRTI耐药,耐药位点均为V179E.结论青岛市MSM新发HIV-1感染者的病毒基因分型复杂多样,存在性活跃人群,尤其要把分型为CRF07_BC、非独居、对传染艾滋病抱有侥幸心理的MSM作为教育的重点,及时阻断传播链,避免分子网络的进一步扩大.展开更多
目的分析安徽省蚌埠市2021-2022年新报告HIV感染者基因亚型、治疗前耐药特征和HIV分子传播网络情况,为当地制定干预措施提供依据。方法收集蚌埠市2021-2022年新报告HIV感染者样本90份,扩增pol基因并测序,借助Blast HIV sequence databas...目的分析安徽省蚌埠市2021-2022年新报告HIV感染者基因亚型、治疗前耐药特征和HIV分子传播网络情况,为当地制定干预措施提供依据。方法收集蚌埠市2021-2022年新报告HIV感染者样本90份,扩增pol基因并测序,借助Blast HIV sequence database对每条序列进行同源性分析,采用MEGA 7.0构建NJ系统进化树判定所有HIV感染者基因亚型。将所得序列上传至美国斯坦福大学HIV耐药数据库,分析基因型耐药情况。利用Cytoscape实现传播网络的可视化,分析新报告HIV感染者耐药传播特点和网络关联情况。结果蚌埠市85例新报告HIV感染者基因亚型主要为CRF01_AE(58.82%)和CRF07_BC(23.53%),耐药率为10.59%(9/85),主要为非核苷类药物耐药(5/85,5.88%)。在0.5%基因距离阈值下的分子传播网络中,CRF01_AE入网率为44%,存在1个较大的耐药传播簇,耐药位点为L33F;CRF07_BC入网率为10%。分子传播网络中的HIV感染者主要为五河县50岁及以上男性,文化程度较低。结论蚌埠市新报告HIV感染者耐药率较高,形成了耐药传播簇,传播风险较高,应采取有效措施进行干预,减少HIV的传播。展开更多
The human immunodeficiency virus(HIV)-infected population with a transmission link has a strong genetic similarity in viral gene sequences that tend to cluster.In recent years,a strategy for guiding HIV/acquired immun...The human immunodeficiency virus(HIV)-infected population with a transmission link has a strong genetic similarity in viral gene sequences that tend to cluster.In recent years,a strategy for guiding HIV/acquired immunodeficiency syndrome(AIDS)prevention using sequence-based transmission networks has been proposed.The execution of this strategy provides an opportunity to effectively control the spread of the AIDS epidemic in China.HIV molecular transmission network research has been actively pursued by a number of Chinese research groups.Medium-and long-term strategies were created with the goal of using HIV molecular transmission networks to guide targeted prevention and control in China.Technical guidelines for HIV transmission network monitoring and intervention have been written and released.Pilot studies are continuing in the meantime.Despite the fact that this strategy has a bright future,there are still many challenges to overcome to achieve the intended AIDS prevention and control goals.展开更多
文摘目的了解青岛市男男性行为者(men who have sex with men,MSM)新发HIV-1感染者的病毒基因分型特征,构建HIV-1分子传播网络,为精准干预提供依据.方法对2022年青岛市MSM新发HIV-1感染者进行问卷调查并采集血样,提取HIV-1病毒RNA,巢式PCR扩增pol区基因片段并进行测序,确定基因分型,计算基因距离,使用MEGA v11软件、HIV-Trace等构建系统进化树与分子网络,使用SPSS 27.0进行入网影响因素分析.结果共调查158名MSM,采集152人血液样本,成功扩增135条pol区序列,共11种基因分型,主要基因分型为:CRF07_BC占42.23%(57/135),CRF01_AE占35.56%(48/135),CRF55_01B占9.63%(13/135);检出了较为特殊的复杂重组体CRF01_BC和二代重组体CRF0107.鉴定出13个传播簇,CRF07_BC形成最大的传播簇,包含43个节点,CRF01_AE包含15个节点,CRF55_01B包含11个节点.在0.03%的基因距离阈值下构建分子网络,入网率为51.11%(69/135).经χ^(2)检验与Fisher精确检验显示,HIV-1基因分型(χ^(2)=44.691,P<0.01)、是否独居(χ^(2)=4.972,P=0.026)、对艾滋病了解程度(P=0.005)进入分子网络的差异有统计学意义.检测出44条序列存在耐药突变位点,耐药突变率为32.59%(44/135),21例进入分子网络.在基因型为CRF55_01B的一个传播簇中,所有感染者均对NNRTI耐药,耐药位点均为V179E.结论青岛市MSM新发HIV-1感染者的病毒基因分型复杂多样,存在性活跃人群,尤其要把分型为CRF07_BC、非独居、对传染艾滋病抱有侥幸心理的MSM作为教育的重点,及时阻断传播链,避免分子网络的进一步扩大.
文摘目的分析安徽省蚌埠市2021-2022年新报告HIV感染者基因亚型、治疗前耐药特征和HIV分子传播网络情况,为当地制定干预措施提供依据。方法收集蚌埠市2021-2022年新报告HIV感染者样本90份,扩增pol基因并测序,借助Blast HIV sequence database对每条序列进行同源性分析,采用MEGA 7.0构建NJ系统进化树判定所有HIV感染者基因亚型。将所得序列上传至美国斯坦福大学HIV耐药数据库,分析基因型耐药情况。利用Cytoscape实现传播网络的可视化,分析新报告HIV感染者耐药传播特点和网络关联情况。结果蚌埠市85例新报告HIV感染者基因亚型主要为CRF01_AE(58.82%)和CRF07_BC(23.53%),耐药率为10.59%(9/85),主要为非核苷类药物耐药(5/85,5.88%)。在0.5%基因距离阈值下的分子传播网络中,CRF01_AE入网率为44%,存在1个较大的耐药传播簇,耐药位点为L33F;CRF07_BC入网率为10%。分子传播网络中的HIV感染者主要为五河县50岁及以上男性,文化程度较低。结论蚌埠市新报告HIV感染者耐药率较高,形成了耐药传播簇,传播风险较高,应采取有效措施进行干预,减少HIV的传播。
基金supported by the Ministry of Science and Technology of China(2017ZX10201101002-004 and 2018ZX10721102-006)the China Key Project of the State Key Laboratory of Infectious Diseases Control and Prevention Chinese State Key Laboratory for Infectious Disease and Develop Grant(2019SKLID602).
文摘The human immunodeficiency virus(HIV)-infected population with a transmission link has a strong genetic similarity in viral gene sequences that tend to cluster.In recent years,a strategy for guiding HIV/acquired immunodeficiency syndrome(AIDS)prevention using sequence-based transmission networks has been proposed.The execution of this strategy provides an opportunity to effectively control the spread of the AIDS epidemic in China.HIV molecular transmission network research has been actively pursued by a number of Chinese research groups.Medium-and long-term strategies were created with the goal of using HIV molecular transmission networks to guide targeted prevention and control in China.Technical guidelines for HIV transmission network monitoring and intervention have been written and released.Pilot studies are continuing in the meantime.Despite the fact that this strategy has a bright future,there are still many challenges to overcome to achieve the intended AIDS prevention and control goals.
