Background: HIV-2 is comparatively less pathogenic with slow progression of infection to clinical disease and consequently there is less of information on the occurrence of HIV-2 associated disease than HIV-1. We here...Background: HIV-2 is comparatively less pathogenic with slow progression of infection to clinical disease and consequently there is less of information on the occurrence of HIV-2 associated disease than HIV-1. We hereby describe some laboratory profiles of individuals presenting with HIV-2 and dual HIV-1/2 related AIDS at the University College hospital in Ibadan over a period of seven years. Methodology: Blood samples from patients presenting with the AIDS defining illness at the University College Hospital, Ibadan, Nigeria were tested for antibodies to HIV-1/2 using rapid test devices or ELISA. Initially reactive samples were further tested by immunoblotting for differentiation into HIV-1 or HIV-2 or HIV-1/2 dual infection. Blood samples from individuals with confirmed infections were further analyzed for CD4 cell lymphocyte number, plasma HIV-1 RNA concentration, hematological and blood chemistry parameters. The data analysis was done using descriptive statistics and Levene-S test for equality of variance. Results: Thirty five patients, 18 and 17 with HIV-2 and dual HIV-1/2 infections respectively were identified during the period covered by this study (2005-2012). The median age of the patients was 48 years old (Range: 42 - 70 years old) and mean CD4 cell count of HIV-2 patients at enrollment was 324 (Range: 16 - 696) and 350 (Range 54 - 863) per microlitre of blood for patients with dual HIV-1/2 infection. HIV-1 RNA was not detected in the plasma of the 18 patients with serological HIV-2 infection but 2 (11.8%) of the 17 patients with dual HIV-1/2 serological profile had detectable HIV-1 RNA (1,287,275 copies/ml and 1,816,491 copies/ml). Conclusion: The results emphasize the need to consider HIV-2 infection in the investigation of patients presenting with the AIDS related illness but with negative HIV-1serology. The study also shows the importance of inclusion of multispot HIV-1 and 2 rapid tests for differentiating HIV-1 from HIV-2 infections in regions where both types of HIV circulate or epidemiologically indicated.展开更多
目的观察中国HIV-1感染者在高效抗反转录病毒治疗（Highly active antiretroviral therapy，HAART）中，外周血HIV-1RNA、CD4＋T淋巴细胞数量和IL-2、IL-7的相关关系，探讨这些^y链细胞因子与抗HIV病毒免疫应答及其在病毒控制中的作用...目的观察中国HIV-1感染者在高效抗反转录病毒治疗（Highly active antiretroviral therapy，HAART）中，外周血HIV-1RNA、CD4＋T淋巴细胞数量和IL-2、IL-7的相关关系，探讨这些^y链细胞因子与抗HIV病毒免疫应答及其在病毒控制中的作用。方法2009年6月至2010年12月，35椤iI接受初次HAART的慢性HIV-1感染者被纳入本研究并随访48周，检测HAART治疗0、24、48周时的外周血HIV．1RNA定量、CD4＋T淋巴细胞数、IL-2以及IL-7水平，并分析其相关性。结果35例HIV．AIDS在HAART治疗前的IL-2水平[（9．67±2．6）pg／ml]明显低于正常对照值[（27．36±5．05）pg／ml]，在经过48周治疗后显著升高[（19．8±3．3）pg／ml]，而在HAART治疗前的IL-7水平[（81．74±20．47）pg／ml]明显高于正常对照值[（2．06±1．52）pg／ml]，在48周治疗后则显著降低[（8．36±2．16）pg／ml]。IL-2水平在HAART的0、48周与CD4＋细胞计数呈相同变化趋势但相关性不明显（O周：R=0．21，P=0．063；48周：R=0．19，P=0．103），24周呈正相关（R=0．24，P=0．033），而IL-7水平在HAART的0周与CD4＋细胞计数呈负相关（R=-0．28，P=0．012），在24、48周无相关。IL-2水平和HIVRNA病毒载量呈负相关（R=-0．17，P=0．032），而IL-7水平和HIV RNA病毒载量关系不明显（P=0．76）。结论48周HAART治疗中IL-2和IL-7水平均有明显变化。与CD4＋T细胞计数变化密切相关，与外周血HIV RNA水平有一定关系。提示这些细胞因子在免疫重建和病毒控制中起重要作用。展开更多
文摘Background: HIV-2 is comparatively less pathogenic with slow progression of infection to clinical disease and consequently there is less of information on the occurrence of HIV-2 associated disease than HIV-1. We hereby describe some laboratory profiles of individuals presenting with HIV-2 and dual HIV-1/2 related AIDS at the University College hospital in Ibadan over a period of seven years. Methodology: Blood samples from patients presenting with the AIDS defining illness at the University College Hospital, Ibadan, Nigeria were tested for antibodies to HIV-1/2 using rapid test devices or ELISA. Initially reactive samples were further tested by immunoblotting for differentiation into HIV-1 or HIV-2 or HIV-1/2 dual infection. Blood samples from individuals with confirmed infections were further analyzed for CD4 cell lymphocyte number, plasma HIV-1 RNA concentration, hematological and blood chemistry parameters. The data analysis was done using descriptive statistics and Levene-S test for equality of variance. Results: Thirty five patients, 18 and 17 with HIV-2 and dual HIV-1/2 infections respectively were identified during the period covered by this study (2005-2012). The median age of the patients was 48 years old (Range: 42 - 70 years old) and mean CD4 cell count of HIV-2 patients at enrollment was 324 (Range: 16 - 696) and 350 (Range 54 - 863) per microlitre of blood for patients with dual HIV-1/2 infection. HIV-1 RNA was not detected in the plasma of the 18 patients with serological HIV-2 infection but 2 (11.8%) of the 17 patients with dual HIV-1/2 serological profile had detectable HIV-1 RNA (1,287,275 copies/ml and 1,816,491 copies/ml). Conclusion: The results emphasize the need to consider HIV-2 infection in the investigation of patients presenting with the AIDS related illness but with negative HIV-1serology. The study also shows the importance of inclusion of multispot HIV-1 and 2 rapid tests for differentiating HIV-1 from HIV-2 infections in regions where both types of HIV circulate or epidemiologically indicated.
