A novel hydrogen peroxide(H2O2)biosensor was prepared.First,zirconium dioxide(ZrO2)were solubilized in the aqueous solution of a biopolymer chitosan(CS)and the mutlicarbon nanotubes(MWNTs)was introduced into the ZrO2-...A novel hydrogen peroxide(H2O2)biosensor was prepared.First,zirconium dioxide(ZrO2)were solubilized in the aqueous solution of a biopolymer chitosan(CS)and the mutlicarbon nanotubes(MWNTs)was introduced into the ZrO2-CS solution to get a ZrO2-CS-MWNTs composite.Then the ZrO2-CS-MWNTs composite was immobilized onto the surface of the glass carbon electrode(GCE).Subquently,a stable nano-Au film was electrodeposited on the ZrO2-CS-MWNTs modified electrode at a constant potential.Finally,horseradish peroxidase(HRP)was immobilized onto the nano-Au layer to obtain the HRP/nano-Au/ ZrO2-CS-MWNTs /GCE electrode.The results showed that the biosensor exhibited well reduction ability for hydrogen peroxide with high sensitivity and fastresponse time resulting from.The biosensor displayed a rapid response to H2O2 and the linear range of the biosensor was from 7.80×10-6 to 9.26×10-3 mol/L with the detection limit of 2.8×10-6 mol/L(S/N=3).In addition,the biosensor showed fast response,high sensitivity,good stability and reproducibility.展开更多
Animals exhibit behavioral differences in their sensitivity to ethanol,a trait that is at least in part due to genetic predispositions.This study has implicated a large neuronal protein involving Highwire,a Drosophila...Animals exhibit behavioral differences in their sensitivity to ethanol,a trait that is at least in part due to genetic predispositions.This study has implicated a large neuronal protein involving Highwire,a Drosophila E3 ubiquitin ligase(Hiw,a homolog of Pam,a protein associated with Myc found in humans)in acute sensitivity to ethanol sedation.Flies lacking Hiw were hypersensitive to the sedating effect of ethanol whereas those overexpressing Hiw showed decreased sensitivity to ethanol.Furthermore,RNAi functional knockdown of Hiw in adult neurons or ellipsoid body neurons showed increased sensitivity to ethanol sedation.None of these manipulations of the hiw gene caused changes in the rate of ethanol absorption and/or metabolism.These results suggest a previously unknown role for this highly conserved gene in regulating the behavioral responses to an addictive drug.展开更多
文摘A novel hydrogen peroxide(H2O2)biosensor was prepared.First,zirconium dioxide(ZrO2)were solubilized in the aqueous solution of a biopolymer chitosan(CS)and the mutlicarbon nanotubes(MWNTs)was introduced into the ZrO2-CS solution to get a ZrO2-CS-MWNTs composite.Then the ZrO2-CS-MWNTs composite was immobilized onto the surface of the glass carbon electrode(GCE).Subquently,a stable nano-Au film was electrodeposited on the ZrO2-CS-MWNTs modified electrode at a constant potential.Finally,horseradish peroxidase(HRP)was immobilized onto the nano-Au layer to obtain the HRP/nano-Au/ ZrO2-CS-MWNTs /GCE electrode.The results showed that the biosensor exhibited well reduction ability for hydrogen peroxide with high sensitivity and fastresponse time resulting from.The biosensor displayed a rapid response to H2O2 and the linear range of the biosensor was from 7.80×10-6 to 9.26×10-3 mol/L with the detection limit of 2.8×10-6 mol/L(S/N=3).In addition,the biosensor showed fast response,high sensitivity,good stability and reproducibility.
基金supported by a fellowship from Tai Solarin University of Education.
文摘Animals exhibit behavioral differences in their sensitivity to ethanol,a trait that is at least in part due to genetic predispositions.This study has implicated a large neuronal protein involving Highwire,a Drosophila E3 ubiquitin ligase(Hiw,a homolog of Pam,a protein associated with Myc found in humans)in acute sensitivity to ethanol sedation.Flies lacking Hiw were hypersensitive to the sedating effect of ethanol whereas those overexpressing Hiw showed decreased sensitivity to ethanol.Furthermore,RNAi functional knockdown of Hiw in adult neurons or ellipsoid body neurons showed increased sensitivity to ethanol sedation.None of these manipulations of the hiw gene caused changes in the rate of ethanol absorption and/or metabolism.These results suggest a previously unknown role for this highly conserved gene in regulating the behavioral responses to an addictive drug.
