HKT transport protein is an ion transporter thai exists on plasmalemma and a ubiquitous transmembrane protein responsible for Na ＋ transport and K＋ - Na ＋ symport. HKT transport protein is regulated by the expressi...HKT transport protein is an ion transporter thai exists on plasmalemma and a ubiquitous transmembrane protein responsible for Na ＋ transport and K＋ - Na ＋ symport. HKT transport protein is regulated by the expression of ItKT family genes. The overexpression of HKT family genes can improve activity of Na＋ transport and K＋ - Na＋ s.vmport proteins in plants, lead to Na ＋ recycle, reduce Na ＋ concentration in vivo, maintain K ＋/Na＋ ratio, and ensure normal physiological functions of eells, thereby improving salt toleranee of plants. This paper introduced the discovery and claning of HKT family genes, revealed interactions between HKT family genes and SOS, NHX genes, and summarized the relationship between the structure, function of HKT protein and salt tolerance of plants to clarify the function and mechanism of action of HKT transport protein, aiming at laying the foundation for genetic engineering of plants for salt tolerauee and providing basis for breeding haloduric transgenie plant.展开更多
In order to study the roles of members of HKT gene fanfily in wheat, TaHKT2 ; 2 was isolated by using homologous cloning strategy and screening genomic BAC library. TaHKT2; 2 genes were mapped on chromosomes 7A, 7B an...In order to study the roles of members of HKT gene fanfily in wheat, TaHKT2 ; 2 was isolated by using homologous cloning strategy and screening genomic BAC library. TaHKT2; 2 genes were mapped on chromosomes 7A, 7B and 7D, named as TaHKT2; 2-7A, TaHKT2; 2-7B, and TaHKT2; 2-7D, respectively. TaHKT2 ; 2 and TaHKT2 ; 1 had the same genetic structure, composed of three exons and two introns, and formed a cluster with TaHKT2 ; 1 on the phylogenetic tree of plant HKT transporters. The coding sequences of TaHKT2 ; 2-7A, TaHKT2 ; 2-7B, and TaHKT2 ; 2-7D were 1 602, 1 602 and 1 596 bp long, respectively, but TaHKT2 ;2-7D cDNA sequence was not isolated by RT-PCR in eight wheat varieties. The natural diversity of TaHKT2 ;2 genes was analyzed by cloning and sequencing from 12 wheat varieties. The results showed that TaHKT2;2-7A was found to be more diverse than TaHKT2; 2-7B and TaHKT2; 2-7D. Only a few bases changed in the alleles of TaHKT2 ; 2 genes in wheat. No amino-acid natural variation lay in the P-loops of deduced protein sequences of all alleles of TaHKT2 ; 2 in 12 wheat varieties. The identity of coding sequences was nmch higher than that of 5＇ flanking regions of TaHKT2 ; 2 genes. TaHKT2 ; 2 nfight be selected over the comse of wheat domestication and belonged to domestication gene.展开更多
基金Supported by Graduate Science and Technology Innovation Project of Inner Mongolia Autonomous Region(S2016013604)National Natural Science Foundation of China(31260336)
文摘HKT transport protein is an ion transporter thai exists on plasmalemma and a ubiquitous transmembrane protein responsible for Na ＋ transport and K＋ - Na ＋ symport. HKT transport protein is regulated by the expression of ItKT family genes. The overexpression of HKT family genes can improve activity of Na＋ transport and K＋ - Na＋ s.vmport proteins in plants, lead to Na ＋ recycle, reduce Na ＋ concentration in vivo, maintain K ＋/Na＋ ratio, and ensure normal physiological functions of eells, thereby improving salt toleranee of plants. This paper introduced the discovery and claning of HKT family genes, revealed interactions between HKT family genes and SOS, NHX genes, and summarized the relationship between the structure, function of HKT protein and salt tolerance of plants to clarify the function and mechanism of action of HKT transport protein, aiming at laying the foundation for genetic engineering of plants for salt tolerauee and providing basis for breeding haloduric transgenie plant.
基金Supported by National Key Research and Development Program of China(2016YFD0101802)Modern Agricultural Science and Technology Innovation Engineering Project of Hebei Province(2017038997)National Natural Science Foundation of Hebei Province(C2014106075)
文摘In order to study the roles of members of HKT gene fanfily in wheat, TaHKT2 ; 2 was isolated by using homologous cloning strategy and screening genomic BAC library. TaHKT2; 2 genes were mapped on chromosomes 7A, 7B and 7D, named as TaHKT2; 2-7A, TaHKT2; 2-7B, and TaHKT2; 2-7D, respectively. TaHKT2 ; 2 and TaHKT2 ; 1 had the same genetic structure, composed of three exons and two introns, and formed a cluster with TaHKT2 ; 1 on the phylogenetic tree of plant HKT transporters. The coding sequences of TaHKT2 ; 2-7A, TaHKT2 ; 2-7B, and TaHKT2 ; 2-7D were 1 602, 1 602 and 1 596 bp long, respectively, but TaHKT2 ;2-7D cDNA sequence was not isolated by RT-PCR in eight wheat varieties. The natural diversity of TaHKT2 ;2 genes was analyzed by cloning and sequencing from 12 wheat varieties. The results showed that TaHKT2;2-7A was found to be more diverse than TaHKT2; 2-7B and TaHKT2; 2-7D. Only a few bases changed in the alleles of TaHKT2 ; 2 genes in wheat. No amino-acid natural variation lay in the P-loops of deduced protein sequences of all alleles of TaHKT2 ; 2 in 12 wheat varieties. The identity of coding sequences was nmch higher than that of 5＇ flanking regions of TaHKT2 ; 2 genes. TaHKT2 ; 2 nfight be selected over the comse of wheat domestication and belonged to domestication gene.
