Objective:To compare the genotype frequencies of HLA class-ⅡDRB1 alleles in Giardia(G.)lamblia-infected children.Methods:A total of 490 Egyptian children aged 2-16 years were subjected to microscopic stool examinatio...Objective:To compare the genotype frequencies of HLA class-ⅡDRB1 alleles in Giardia(G.)lamblia-infected children.Methods:A total of 490 Egyptian children aged 2-16 years were subjected to microscopic stool examination to detect G.lamblia infection,and to exclude other intestinal pathogens.On the basis of their microscopic findings,a group of 80 children were chosen as giardiasis cases,another 80 children were confirmed as Giardia free control group by immunochromatographic test,and the remaining children were excluded.Both giardiasis and control groups were then subjected to blood examination to identify their genetic type of HLA-DRB1 alleles.Results:HLA class-ⅡDRB1*03:01 and DRB1*13:01 alleles were significantly associated with G.lamblia infection(P<0.001 for each variable).On the other hand,HLA class-ⅡDRB1*04:02,DRB1*10:01,DRB1*14:01 and DRB1*15:01 alleles were significantly demonstrated in Giardia free children.However,other HLA-DRB1 alleles did not show any significant association with giardiasis.Conclusions:HLA class-ⅡDRB1*03,DRB1*13,DRB1*04,DRB1*10,DRB1*14 and DRB1*15 alleles may be involved in the establishment of host immune response to G.lamblia infection.展开更多
Background: In Iranian patients with opticospinal multiple sclerosis (OSMS), a paucity of brain lesions and short spinal cord lesions extending less than three spinal segments are characteristic findings on magnetic r...Background: In Iranian patients with opticospinal multiple sclerosis (OSMS), a paucity of brain lesions and short spinal cord lesions extending less than three spinal segments are characteristic findings on magnetic resonance imaging (MRI). It also shows a relatively benign course with negative CSF oligo-coonal bands. Objective: We aimed to clarify the possible relationship between clinical phenotype and MRI features of OSMS and human leucocyte antigen (HLA) system in Iran. Methods: Genotyping of HLA class II allele frequencies in 20 patients with OSMS were done, using polymerase chain reaction sequence-specific primer amplification method. Blood samples were extracted and typed for HLA-DRB, DQA, and DQB loci and compared with 100 controls. Results: Significant positive association was observed in DRB1*03, DQA1*0201, DQA1*03, DQB1*0201, and DQB1*0611, while DQB1*0602 was absent in our patients. Conclusion: These finding suggest that HLA-DRB association pattern in OSMS is different from conventional MS in Iran which is mostly associated with DRB1*1501 and from similar Japanese OSMS who are negative for brain lesions fulfilling the Barkhof criteria and negative for the presence of longitudinally extensive spinal cord lesions who carries the DRB*0405 allele. OSMS is immunogenetically heterogeneous. Also absence of DQB1*0602 allele may negatively be associated with the absence of Barkhof brain lesion.展开更多
Objective To investigate the association of major histocompatibility complex (MHC) alleles with the susceptibility of systemic lupus orythematosus (SLE) in Chinese Han nationality. Methods Genotyping of HLA class ...Objective To investigate the association of major histocompatibility complex (MHC) alleles with the susceptibility of systemic lupus orythematosus (SLE) in Chinese Han nationality. Methods Genotyping of HLA class Ⅱ alleles was performed by polymerase chain reaction and sequence specific oligonucleotide (PCR/SSO) probe hybridizations. The oligonucleotide probes were DIG ddUTP labeled. Chemiluminescent detection method was used. A total of 157 Chinese patients with SLE, their 110 first degree relatives and 160 healthy controls were studied. The subjects mainly came from east China and all were Han nationality. Cases and controls were matched in ethnicity. Transmission/disequilibrium test was performed on 33 nuclear pedigrees of SLE patients, using the TDTEX program of S.A.G.E. (Statistical Analysis for Genetic Epidemiology, version 3.0). Results DR2 was increased in patients P<0.0002, Odds ratio (OR)=2.58)]. Among all the 27 alleles, only DQA1*0102 (OR=2.43) and DRB1*1501 (OR= 2.58) were significantly increased in patients after correction (Pc<0.02). DQB1*0502 and *0602 (both P<0.005, Pc>0.05), DRB1*1602 (P< 0.03 , Pc>0.05) were increased in patients but not significant after correction. In contrast to the above alleles, DQB1*0301 (P<0.05, Pc>0.05, OR= 0.60 ), and DQB1*0302 (P<0.003, Pc>0.05, OR=0.29) were decreased Department of Epidemiology, Shanghai Medical University, Shanghai 200032, China (Shen FM and Zhang WH) Department of Maternal and Child Health, Shanghai Medical University, Shanghai, China (Meng W) Abstract in patients as compared with controls, but not significant after correction. Three point haplotype HLA DRB1*1501 DQA1*0102 DQB1*0602 was increased in patients (P<0.004, Pc<0.05, OR=2.49) as compared with controls and increased in probands with family history of SLE as compared with those without. Other two DR2 haplotypes, HLA DRB1*1602 DQA1*0102 DQB1*0502 and HLA DRB1*1501 DQA1* 0102 DQB1*0502 were also increased in patients with marginal significance, we call these three haplotypes susceptibility haplotypes. Familial study showed that these susceptibility haplotypes transmitted more frequently from fathers than from mothers. TDT analysis showed that there was linkage between DQA1, DQB1 alleles and the susceptibility gene of SLE (P>0.025 and P<0.05, respectively). We could not do TDT analysis for DRB1 locus due to the lack of genotyping data for all of the DRB1 alleles. Several autoantibodies were associated with HLA alleles. ACL and Anti RNP autoantibodies were positively associated with haplotypes DRB1*0901 DQB1*0303 and DRB1* 07 DQA1*0201 DQB1*0201, respectively. Conclusions HLA class II alleles are associated with the genetic susceptibility of SLE. There is linkage between HLA DQA1, DQB1 (and possibly DRB1 if we do the analysis) alleles and susceptibility gene associated with SLE. The susceptibility gene(s) of SLE in this region is (are) located in DR2 haplotypes and most likely between DRB1 and DQA1 loci.展开更多
文摘Objective:To compare the genotype frequencies of HLA class-ⅡDRB1 alleles in Giardia(G.)lamblia-infected children.Methods:A total of 490 Egyptian children aged 2-16 years were subjected to microscopic stool examination to detect G.lamblia infection,and to exclude other intestinal pathogens.On the basis of their microscopic findings,a group of 80 children were chosen as giardiasis cases,another 80 children were confirmed as Giardia free control group by immunochromatographic test,and the remaining children were excluded.Both giardiasis and control groups were then subjected to blood examination to identify their genetic type of HLA-DRB1 alleles.Results:HLA class-ⅡDRB1*03:01 and DRB1*13:01 alleles were significantly associated with G.lamblia infection(P<0.001 for each variable).On the other hand,HLA class-ⅡDRB1*04:02,DRB1*10:01,DRB1*14:01 and DRB1*15:01 alleles were significantly demonstrated in Giardia free children.However,other HLA-DRB1 alleles did not show any significant association with giardiasis.Conclusions:HLA class-ⅡDRB1*03,DRB1*13,DRB1*04,DRB1*10,DRB1*14 and DRB1*15 alleles may be involved in the establishment of host immune response to G.lamblia infection.
文摘Background: In Iranian patients with opticospinal multiple sclerosis (OSMS), a paucity of brain lesions and short spinal cord lesions extending less than three spinal segments are characteristic findings on magnetic resonance imaging (MRI). It also shows a relatively benign course with negative CSF oligo-coonal bands. Objective: We aimed to clarify the possible relationship between clinical phenotype and MRI features of OSMS and human leucocyte antigen (HLA) system in Iran. Methods: Genotyping of HLA class II allele frequencies in 20 patients with OSMS were done, using polymerase chain reaction sequence-specific primer amplification method. Blood samples were extracted and typed for HLA-DRB, DQA, and DQB loci and compared with 100 controls. Results: Significant positive association was observed in DRB1*03, DQA1*0201, DQA1*03, DQB1*0201, and DQB1*0611, while DQB1*0602 was absent in our patients. Conclusion: These finding suggest that HLA-DRB association pattern in OSMS is different from conventional MS in Iran which is mostly associated with DRB1*1501 and from similar Japanese OSMS who are negative for brain lesions fulfilling the Barkhof criteria and negative for the presence of longitudinally extensive spinal cord lesions who carries the DRB*0405 allele. OSMS is immunogenetically heterogeneous. Also absence of DQB1*0602 allele may negatively be associated with the absence of Barkhof brain lesion.
文摘Objective To investigate the association of major histocompatibility complex (MHC) alleles with the susceptibility of systemic lupus orythematosus (SLE) in Chinese Han nationality. Methods Genotyping of HLA class Ⅱ alleles was performed by polymerase chain reaction and sequence specific oligonucleotide (PCR/SSO) probe hybridizations. The oligonucleotide probes were DIG ddUTP labeled. Chemiluminescent detection method was used. A total of 157 Chinese patients with SLE, their 110 first degree relatives and 160 healthy controls were studied. The subjects mainly came from east China and all were Han nationality. Cases and controls were matched in ethnicity. Transmission/disequilibrium test was performed on 33 nuclear pedigrees of SLE patients, using the TDTEX program of S.A.G.E. (Statistical Analysis for Genetic Epidemiology, version 3.0). Results DR2 was increased in patients P<0.0002, Odds ratio (OR)=2.58)]. Among all the 27 alleles, only DQA1*0102 (OR=2.43) and DRB1*1501 (OR= 2.58) were significantly increased in patients after correction (Pc<0.02). DQB1*0502 and *0602 (both P<0.005, Pc>0.05), DRB1*1602 (P< 0.03 , Pc>0.05) were increased in patients but not significant after correction. In contrast to the above alleles, DQB1*0301 (P<0.05, Pc>0.05, OR= 0.60 ), and DQB1*0302 (P<0.003, Pc>0.05, OR=0.29) were decreased Department of Epidemiology, Shanghai Medical University, Shanghai 200032, China (Shen FM and Zhang WH) Department of Maternal and Child Health, Shanghai Medical University, Shanghai, China (Meng W) Abstract in patients as compared with controls, but not significant after correction. Three point haplotype HLA DRB1*1501 DQA1*0102 DQB1*0602 was increased in patients (P<0.004, Pc<0.05, OR=2.49) as compared with controls and increased in probands with family history of SLE as compared with those without. Other two DR2 haplotypes, HLA DRB1*1602 DQA1*0102 DQB1*0502 and HLA DRB1*1501 DQA1* 0102 DQB1*0502 were also increased in patients with marginal significance, we call these three haplotypes susceptibility haplotypes. Familial study showed that these susceptibility haplotypes transmitted more frequently from fathers than from mothers. TDT analysis showed that there was linkage between DQA1, DQB1 alleles and the susceptibility gene of SLE (P>0.025 and P<0.05, respectively). We could not do TDT analysis for DRB1 locus due to the lack of genotyping data for all of the DRB1 alleles. Several autoantibodies were associated with HLA alleles. ACL and Anti RNP autoantibodies were positively associated with haplotypes DRB1*0901 DQB1*0303 and DRB1* 07 DQA1*0201 DQB1*0201, respectively. Conclusions HLA class II alleles are associated with the genetic susceptibility of SLE. There is linkage between HLA DQA1, DQB1 (and possibly DRB1 if we do the analysis) alleles and susceptibility gene associated with SLE. The susceptibility gene(s) of SLE in this region is (are) located in DR2 haplotypes and most likely between DRB1 and DQA1 loci.
