Background:HLA-DMA presents pathogen-derived antigens to CD4+and CD8+T cells,respectively,and plays a significant part in initiating the immune response.So far,the impact of HLA expression on the prognosis of BC cells...Background:HLA-DMA presents pathogen-derived antigens to CD4+and CD8+T cells,respectively,and plays a significant part in initiating the immune response.So far,the impact of HLA expression on the prognosis of BC cells is controversial,because few studies have shown that the expressions of some HLA genes are related to the improvement of the survival rate.Up till now,however,the relationship between HLA-DMA and LUAD has not yet been assessed.Methods:We analyzed the TCGA database and assessed the prognostic value of HLA-DMA in LUAD.We conducted the Kruskal–Wallis and Wilcoxon signed-rank test and utilized logistic regression to assess the role of clinical-pathologic characteristics and HLA-DMA expression.Kaplan–Meier method and the multivariate and univariate Cox regression were also used for evaluating the prognosis-related factors of LUAD.GSEA was used to identify HLA-DMA-related key pathways.The ssGSEA of the TCGA data was used to investigate the correlations between HLA-DMA and cancer immune cell infiltration.Results:Low HLA-DMA expression was related to poorer disease-specific survival(DSS)and overall survival(OS)of LUAD patients.GSEA revealed that HLA-DMA was tightly interrelated with an immune response by the reactome activation of anterior hox genes in hindbrain development during the early embryogenesis signaling pathway.The expression of HLA-DMA was positively associated with cytotoxic cell infiltration and negatively related to the Th2 cell infiltration according to the ssGSEA.Western blotting and the CCK-8 assay showed that KD-HLA-DMA could significantly increase the proliferation of A549 cells and significantly reduce cell pyroptosis.Conclusion:All the observations implied that HLA-DMA was associated with patient prognosis and immune infiltration in LUAD.展开更多
目的采用高分辨融解曲线(HRM)技术建立检测类ADP核糖基化因子GTP酶15(ARL15)、主要组织相容性抗原复合体ⅡDMα(HLA-DMA)和核因子κB亚基2(NFKB2)基因单核苷酸多态性(SNP)的方法,并探讨其与中国西北地区汉族人群类风湿性关节炎(RA)易感...目的采用高分辨融解曲线(HRM)技术建立检测类ADP核糖基化因子GTP酶15(ARL15)、主要组织相容性抗原复合体ⅡDMα(HLA-DMA)和核因子κB亚基2(NFKB2)基因单核苷酸多态性(SNP)的方法,并探讨其与中国西北地区汉族人群类风湿性关节炎(RA)易感性的关系。方法针对rs255758、rs1063478、rs397514331和rs397514332四个SNP位点建立PCRHRM检测体系并测序验证。对588例RA患者和200例健康对照标本进行病例对照研究,分析这四个SNP与RA发病风险的关系。结果建立的针对四个SNP位点的PCR-HRM基因分型方法经测序验证可正确分型。rs397514331和rs397514332位点未发现突变基因型。rs255758和rs1063478位点的基因型频率在两组间存在统计学差异,而基因频率无统计学差异。其中rs255758位点的AA基因型在显性模型(AA vs AC/CC)下降低RA发病风险(OR=0.666,95%CI=0.478~0.927,P=0.016)。结论我们建立的PCR-HRM基因分型方法可对rs255758、rs1063478、rs397514331和rs397514332四个SNP位点进行常规化检测。ARL15和HLA-DMA基因多态性与中国西北地区汉族人群RA易感相关。展开更多
基金the National Natural Science Foundation of China(Grant No.81971036)China Medical and Health Development Foundation(Grant Nos.C202212-006,C202212-0014).
文摘Background:HLA-DMA presents pathogen-derived antigens to CD4+and CD8+T cells,respectively,and plays a significant part in initiating the immune response.So far,the impact of HLA expression on the prognosis of BC cells is controversial,because few studies have shown that the expressions of some HLA genes are related to the improvement of the survival rate.Up till now,however,the relationship between HLA-DMA and LUAD has not yet been assessed.Methods:We analyzed the TCGA database and assessed the prognostic value of HLA-DMA in LUAD.We conducted the Kruskal–Wallis and Wilcoxon signed-rank test and utilized logistic regression to assess the role of clinical-pathologic characteristics and HLA-DMA expression.Kaplan–Meier method and the multivariate and univariate Cox regression were also used for evaluating the prognosis-related factors of LUAD.GSEA was used to identify HLA-DMA-related key pathways.The ssGSEA of the TCGA data was used to investigate the correlations between HLA-DMA and cancer immune cell infiltration.Results:Low HLA-DMA expression was related to poorer disease-specific survival(DSS)and overall survival(OS)of LUAD patients.GSEA revealed that HLA-DMA was tightly interrelated with an immune response by the reactome activation of anterior hox genes in hindbrain development during the early embryogenesis signaling pathway.The expression of HLA-DMA was positively associated with cytotoxic cell infiltration and negatively related to the Th2 cell infiltration according to the ssGSEA.Western blotting and the CCK-8 assay showed that KD-HLA-DMA could significantly increase the proliferation of A549 cells and significantly reduce cell pyroptosis.Conclusion:All the observations implied that HLA-DMA was associated with patient prognosis and immune infiltration in LUAD.
文摘目的采用高分辨融解曲线(HRM)技术建立检测类ADP核糖基化因子GTP酶15(ARL15)、主要组织相容性抗原复合体ⅡDMα(HLA-DMA)和核因子κB亚基2(NFKB2)基因单核苷酸多态性(SNP)的方法,并探讨其与中国西北地区汉族人群类风湿性关节炎(RA)易感性的关系。方法针对rs255758、rs1063478、rs397514331和rs397514332四个SNP位点建立PCRHRM检测体系并测序验证。对588例RA患者和200例健康对照标本进行病例对照研究,分析这四个SNP与RA发病风险的关系。结果建立的针对四个SNP位点的PCR-HRM基因分型方法经测序验证可正确分型。rs397514331和rs397514332位点未发现突变基因型。rs255758和rs1063478位点的基因型频率在两组间存在统计学差异,而基因频率无统计学差异。其中rs255758位点的AA基因型在显性模型(AA vs AC/CC)下降低RA发病风险(OR=0.666,95%CI=0.478~0.927,P=0.016)。结论我们建立的PCR-HRM基因分型方法可对rs255758、rs1063478、rs397514331和rs397514332四个SNP位点进行常规化检测。ARL15和HLA-DMA基因多态性与中国西北地区汉族人群RA易感相关。
