Background: Vitiligo is an acquired depigmentary disorder of the skin and hair which results from selective destruction of melanocytes. Serological typing and genotyping of human leukocyte antigen (HLA) have shown dis...Background: Vitiligo is an acquired depigmentary disorder of the skin and hair which results from selective destruction of melanocytes. Serological typing and genotyping of human leukocyte antigen (HLA) have shown discrepancies in HLA associations with vitiligo in different ethnic populations. Methods: Polymerase chain reaction sequence-specific primer (PCR-SSP) method was used to analyze the distribution of HLA-DQA1, and -DQB1, alleles among 187 patients with vitiligo and 273 healthy controls through Epi Info version 6 package (Centers for Disease Control and Prevention, Atlanta, GA, USA). Results: The frequencies of HLA-DQA1 0302(OR = 1.98, Pc< 0.01), -DQB1 0303 (OR = 3.14, Pc< 0.001), and -DQB1 0503 (OR = 3.36, Pc < 0.05) alleles were significantly increased in patients with vitiligo compared with controls, and HLA-DQA1 0501 (OR = 0.40, Pc < 0.01) allele frequencywas highly decreased. HLA-DQA1 0302 (OR = 5.19, Pc < 0.001), -DQA1 0601 (OR= 2.95,Pc <0.05), -DQB1 0303 (OR= 4.50, Pc < 0.001), and -DQB1 0503 (OR = 6.69, Pc < 0.001) alleles were positively associated, whereas HLA-DQA1 0501 (OR = 0.05, Pc < 0.001) allele was negatively associated with childhood vitiligo patients, and HLA-DQB1 0303 (OR = 2.76, Pc < 0.001) allele was positively associated with adult vitiligo patients compared with controls. The frequency of HLA-DQB1 0303 (OR = 3.72, Pc < 0.001) allele was significantly increased in localized vitiligo patients vs. controls, whereas HLA-DQA1 0302 (OR = 2.47, Pc < 0.01), -DQB1 0303 (OR = 2.67, Pc <0.01), and -DQB1 0503 (OR = 4.46, Pc <0.01) allele frequencies were significantly increased and -DQA1 0501 (OR = 0.27, Pc <0.01) allele frequency was highly decreased in generalized vitiligo patients. Conclusions: HLA-DQA1 0302, DQA1 0601, -DQB1 0303, and -DQB1 0503 alleles could be susceptible alleles of vitiligo, while HLA-DQA1 0501 allele could be a protective allele in Chinese Hans. There may be different genetic backgrounds between vitiligo patients of childhood and adult, localized and generalized.展开更多
目的从基因水平探讨湖北地区汉族人食管癌 HEN-DQB1等位基因的遗传易感性.方法运用序列特异性引物聚合酶链反应技术,检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的 HLA-DQB1等位基因.SAS system 统计软件数据处理.结果湖北汉...目的从基因水平探讨湖北地区汉族人食管癌 HEN-DQB1等位基因的遗传易感性.方法运用序列特异性引物聚合酶链反应技术,检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的 HLA-DQB1等位基因.SAS system 统计软件数据处理.结果湖北汉族人食管癌患者与正常人比较,HEN-DQB1*0301基因频率显著增高(0.2976 vs 0.1875),P=0.046,OR=1.835,病因分数=0.1354);两组间 HLA-DQB1其余各等位基因分布频率的比较,HLA-DQB1*0201(0.0833 vs 0.1016),*0301(0.2976 vs 0.1875),*0302(0.0595 vs 0859),*0303(0.2381 vs 0.1875),*0304(0.0000 vs 0.0039),*0401(0.0714 vs 0.0469),*0402(0.0119 vs 0.0156),*0501(0.0357 vs 0.0703),*0502(0.0595 vs 0.0664),*0503(0.0119 vs 0.0195),*0504(0.0000 vs 0.0039),*0601(0.0595 vs 0.0781),*0602(0.0476 vs 0.0742),*0603(0.0000 vs 0.0078),*0604(0.0238 vs 0.0508),差异均无显著性.结论 HLA-DQB1*0301等位基因与湖北汉族人食管癌正关联,为其易感基因.展开更多
文摘Background: Vitiligo is an acquired depigmentary disorder of the skin and hair which results from selective destruction of melanocytes. Serological typing and genotyping of human leukocyte antigen (HLA) have shown discrepancies in HLA associations with vitiligo in different ethnic populations. Methods: Polymerase chain reaction sequence-specific primer (PCR-SSP) method was used to analyze the distribution of HLA-DQA1, and -DQB1, alleles among 187 patients with vitiligo and 273 healthy controls through Epi Info version 6 package (Centers for Disease Control and Prevention, Atlanta, GA, USA). Results: The frequencies of HLA-DQA1 0302(OR = 1.98, Pc< 0.01), -DQB1 0303 (OR = 3.14, Pc< 0.001), and -DQB1 0503 (OR = 3.36, Pc < 0.05) alleles were significantly increased in patients with vitiligo compared with controls, and HLA-DQA1 0501 (OR = 0.40, Pc < 0.01) allele frequencywas highly decreased. HLA-DQA1 0302 (OR = 5.19, Pc < 0.001), -DQA1 0601 (OR= 2.95,Pc <0.05), -DQB1 0303 (OR= 4.50, Pc < 0.001), and -DQB1 0503 (OR = 6.69, Pc < 0.001) alleles were positively associated, whereas HLA-DQA1 0501 (OR = 0.05, Pc < 0.001) allele was negatively associated with childhood vitiligo patients, and HLA-DQB1 0303 (OR = 2.76, Pc < 0.001) allele was positively associated with adult vitiligo patients compared with controls. The frequency of HLA-DQB1 0303 (OR = 3.72, Pc < 0.001) allele was significantly increased in localized vitiligo patients vs. controls, whereas HLA-DQA1 0302 (OR = 2.47, Pc < 0.01), -DQB1 0303 (OR = 2.67, Pc <0.01), and -DQB1 0503 (OR = 4.46, Pc <0.01) allele frequencies were significantly increased and -DQA1 0501 (OR = 0.27, Pc <0.01) allele frequency was highly decreased in generalized vitiligo patients. Conclusions: HLA-DQA1 0302, DQA1 0601, -DQB1 0303, and -DQB1 0503 alleles could be susceptible alleles of vitiligo, while HLA-DQA1 0501 allele could be a protective allele in Chinese Hans. There may be different genetic backgrounds between vitiligo patients of childhood and adult, localized and generalized.
文摘目的从基因水平探讨湖北地区汉族人食管癌 HEN-DQB1等位基因的遗传易感性.方法运用序列特异性引物聚合酶链反应技术,检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的 HLA-DQB1等位基因.SAS system 统计软件数据处理.结果湖北汉族人食管癌患者与正常人比较,HEN-DQB1*0301基因频率显著增高(0.2976 vs 0.1875),P=0.046,OR=1.835,病因分数=0.1354);两组间 HLA-DQB1其余各等位基因分布频率的比较,HLA-DQB1*0201(0.0833 vs 0.1016),*0301(0.2976 vs 0.1875),*0302(0.0595 vs 0859),*0303(0.2381 vs 0.1875),*0304(0.0000 vs 0.0039),*0401(0.0714 vs 0.0469),*0402(0.0119 vs 0.0156),*0501(0.0357 vs 0.0703),*0502(0.0595 vs 0.0664),*0503(0.0119 vs 0.0195),*0504(0.0000 vs 0.0039),*0601(0.0595 vs 0.0781),*0602(0.0476 vs 0.0742),*0603(0.0000 vs 0.0078),*0604(0.0238 vs 0.0508),差异均无显著性.结论 HLA-DQB1*0301等位基因与湖北汉族人食管癌正关联,为其易感基因.
