明确不同水氮互作对强筋优质小麦师栾02-1产量和加工品质的影响,为强筋小麦生产中如何通过合理灌溉和优化氮肥施用量来实现协同提高籽粒产量和加工品质的目标提供理论依据。2017-2020年,大田条件下设置浇水次数和施氮量二因子裂区试验,...明确不同水氮互作对强筋优质小麦师栾02-1产量和加工品质的影响,为强筋小麦生产中如何通过合理灌溉和优化氮肥施用量来实现协同提高籽粒产量和加工品质的目标提供理论依据。2017-2020年,大田条件下设置浇水次数和施氮量二因子裂区试验,主区为浇水次数,设春浇一水(W1,拔节水)和春浇两水(W2,拔节水+开花水);副区为氮肥施用量,设N0、N1、N2、N3、N4和N5(0、60、120、180、240和300 kg hm^(-2))6个水平。结果表明,施氮量0~300 kg hm^(-2)时,不同降水年型春浇一水、春浇两水小麦产量随施氮量的增加均先增加后减少,产量最高值对应的施氮量均为240 kg hm^(-2)。施氮量120~300 kg hm^(-2)时,春浇两水处理产量显著高于春浇一水处理。水氮互作对小麦单位面积收获穗数的影响最大,其次是千粒重,对穗粒数的影响最小。施氮量0~300 kg hm^(-2)时,2017-2018年度(丰水年型),春浇两水小麦湿面筋含量、沉降值、吸水率、面团稳定时间、拉伸能量、最大拉伸阻力平均值均高于春浇一水,而2018-2019、2019-2020年度(干旱年型)则相反:春浇一水高于春浇两水。不同降水年型春浇一水、春浇两水小麦湿面筋含量和沉降值随施氮量的增加先增加后减少或逐渐增加,二者最大值对应的施氮量为240 kg hm^(-2)或300 kg hm^(-2);稳定时间、拉伸能量和最大拉伸阻力随施氮量的增加均先增加后减少,施氮量240 kg hm^(-2)时达到最大值。不同降水年型强筋优质小麦师栾02-1生育期春浇两水、施氮量240 kg hm^(-2)时,籽粒产量和加工品质表现最佳。展开更多
Macromolecular crystallography beamline BL17U1 at the Shanghai Synchrotron Radiation Facility has been relocated,upgraded,and given a new ID(BL02U1).It now delivers X-rays in the energy range of 6–16 keV,with a focus...Macromolecular crystallography beamline BL17U1 at the Shanghai Synchrotron Radiation Facility has been relocated,upgraded,and given a new ID(BL02U1).It now delivers X-rays in the energy range of 6–16 keV,with a focused beam of 11.6μm×4.8μm and photon flux greater than 1012 phs/s.The high credibility and stability of the beam and good timing synchronization of the equipment significantly improve the experimental efficiency.Since June 2021,when it officially opened to users,over 4200 h of beamtime have been provided to over 200 research groups to collect data at the beamline.Its good performance and stable operation have led to the resolution of several structures based on data collected at the beamline.展开更多
BACKGROUND Celiac Disease(CD)is an immune-mediated disorder,in which the HLA immunogenetic background(DQ2 and DQ8 heterodimers)and environmental trigger(gluten)are well established.Indeed,both factors are necessary–b...BACKGROUND Celiac Disease(CD)is an immune-mediated disorder,in which the HLA immunogenetic background(DQ2 and DQ8 heterodimers)and environmental trigger(gluten)are well established.Indeed,both factors are necessary–but not sufficient–to develop CD.However,it is very likely that CD is underdiagnosed in both developing and developed countries,due to several aspects,including the fact that a lot of patients present mild and/or atypical symptoms,without the presence of any recognized risk factors.Therefore,the possibility and feasibility of widened screening strategies to identify CD patients are debated.AIM To provide further evidence of the main epidemiological importance of HLADQB1*02 allele in the population of CD patients.METHODS We performed a systematic search in PubMed,EMBASE,Cochrane,Web of Science and Scopus databases,in order to produce a systematic review assessing the carrier frequency of HLA-DQB1*02 allele in the celiac population.Following the PRISMA guidelines,we retrieved all the original articles describing CD patients’HLA-DQB1 genotype in such a way that could allow to assess the HLADQB1*02 carrier frequency among CD patients,along with the evidence of the appropriate diagnostic work-up to achieve a correct and final diagnosis of CD.RESULTS The final output of this systematic search in the medical literature consisted of 38 studies providing the appropriate HLA-DQB1 genotype information of the respective CD population.According to this systematic review,including a pool of 4945 HLA-DQ genotyped CD patients,the HLA-DQB1*02 carrier frequency was 94.94%,meaning that only 5.06%of CD patients were completely lacking this allelic variant.Interestingly,if we consider only the studies whereby the prevalence of CD patients affected with type 1 diabetes mellitus was supposed or clearly established to be very low,the frequency of non-HLA-DQB1*02 carriers among CD patients dropped to 3.65%.CONCLUSION Such a high carrier frequency of the HLA-DQB1*02 allelic variant(which is>95%-96%in CD patients without risk factors,like type 1 diabetes mellitus comorbidity)might be exploited to consider a cost-effective and widened screening approach.If a sustainable strategy could be implemented through a low-cost targeted genetic test to detect the individual presence of HLA-DQB1*02 allele,an appropriate algorithm for serological screening in individuals resulting to be genetically predisposed to CD,might be considered.展开更多
目的从基因水平探讨湖北地区汉族人食管癌 HEN-DQB1等位基因的遗传易感性.方法运用序列特异性引物聚合酶链反应技术,检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的 HLA-DQB1等位基因.SAS system 统计软件数据处理.结果湖北汉...目的从基因水平探讨湖北地区汉族人食管癌 HEN-DQB1等位基因的遗传易感性.方法运用序列特异性引物聚合酶链反应技术,检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的 HLA-DQB1等位基因.SAS system 统计软件数据处理.结果湖北汉族人食管癌患者与正常人比较,HEN-DQB1*0301基因频率显著增高(0.2976 vs 0.1875),P=0.046,OR=1.835,病因分数=0.1354);两组间 HLA-DQB1其余各等位基因分布频率的比较,HLA-DQB1*0201(0.0833 vs 0.1016),*0301(0.2976 vs 0.1875),*0302(0.0595 vs 0859),*0303(0.2381 vs 0.1875),*0304(0.0000 vs 0.0039),*0401(0.0714 vs 0.0469),*0402(0.0119 vs 0.0156),*0501(0.0357 vs 0.0703),*0502(0.0595 vs 0.0664),*0503(0.0119 vs 0.0195),*0504(0.0000 vs 0.0039),*0601(0.0595 vs 0.0781),*0602(0.0476 vs 0.0742),*0603(0.0000 vs 0.0078),*0604(0.0238 vs 0.0508),差异均无显著性.结论 HLA-DQB1*0301等位基因与湖北汉族人食管癌正关联,为其易感基因.展开更多
文摘明确不同水氮互作对强筋优质小麦师栾02-1产量和加工品质的影响,为强筋小麦生产中如何通过合理灌溉和优化氮肥施用量来实现协同提高籽粒产量和加工品质的目标提供理论依据。2017-2020年,大田条件下设置浇水次数和施氮量二因子裂区试验,主区为浇水次数,设春浇一水(W1,拔节水)和春浇两水(W2,拔节水+开花水);副区为氮肥施用量,设N0、N1、N2、N3、N4和N5(0、60、120、180、240和300 kg hm^(-2))6个水平。结果表明,施氮量0~300 kg hm^(-2)时,不同降水年型春浇一水、春浇两水小麦产量随施氮量的增加均先增加后减少,产量最高值对应的施氮量均为240 kg hm^(-2)。施氮量120~300 kg hm^(-2)时,春浇两水处理产量显著高于春浇一水处理。水氮互作对小麦单位面积收获穗数的影响最大,其次是千粒重,对穗粒数的影响最小。施氮量0~300 kg hm^(-2)时,2017-2018年度(丰水年型),春浇两水小麦湿面筋含量、沉降值、吸水率、面团稳定时间、拉伸能量、最大拉伸阻力平均值均高于春浇一水,而2018-2019、2019-2020年度(干旱年型)则相反:春浇一水高于春浇两水。不同降水年型春浇一水、春浇两水小麦湿面筋含量和沉降值随施氮量的增加先增加后减少或逐渐增加,二者最大值对应的施氮量为240 kg hm^(-2)或300 kg hm^(-2);稳定时间、拉伸能量和最大拉伸阻力随施氮量的增加均先增加后减少,施氮量240 kg hm^(-2)时达到最大值。不同降水年型强筋优质小麦师栾02-1生育期春浇两水、施氮量240 kg hm^(-2)时,籽粒产量和加工品质表现最佳。
基金supported by the National Key Research and Development Program of China(No.2021YFC2301405)the National Natural Science Foundation of China(No.31971121)Shanghai Science and Technology Plan Project(No.21ZR14718)。
文摘Macromolecular crystallography beamline BL17U1 at the Shanghai Synchrotron Radiation Facility has been relocated,upgraded,and given a new ID(BL02U1).It now delivers X-rays in the energy range of 6–16 keV,with a focused beam of 11.6μm×4.8μm and photon flux greater than 1012 phs/s.The high credibility and stability of the beam and good timing synchronization of the equipment significantly improve the experimental efficiency.Since June 2021,when it officially opened to users,over 4200 h of beamtime have been provided to over 200 research groups to collect data at the beamline.Its good performance and stable operation have led to the resolution of several structures based on data collected at the beamline.
基金the Nazarbayev University Faculty Development Competitive Research Grant 2020-2022,No.240919FD3912.
文摘BACKGROUND Celiac Disease(CD)is an immune-mediated disorder,in which the HLA immunogenetic background(DQ2 and DQ8 heterodimers)and environmental trigger(gluten)are well established.Indeed,both factors are necessary–but not sufficient–to develop CD.However,it is very likely that CD is underdiagnosed in both developing and developed countries,due to several aspects,including the fact that a lot of patients present mild and/or atypical symptoms,without the presence of any recognized risk factors.Therefore,the possibility and feasibility of widened screening strategies to identify CD patients are debated.AIM To provide further evidence of the main epidemiological importance of HLADQB1*02 allele in the population of CD patients.METHODS We performed a systematic search in PubMed,EMBASE,Cochrane,Web of Science and Scopus databases,in order to produce a systematic review assessing the carrier frequency of HLA-DQB1*02 allele in the celiac population.Following the PRISMA guidelines,we retrieved all the original articles describing CD patients’HLA-DQB1 genotype in such a way that could allow to assess the HLADQB1*02 carrier frequency among CD patients,along with the evidence of the appropriate diagnostic work-up to achieve a correct and final diagnosis of CD.RESULTS The final output of this systematic search in the medical literature consisted of 38 studies providing the appropriate HLA-DQB1 genotype information of the respective CD population.According to this systematic review,including a pool of 4945 HLA-DQ genotyped CD patients,the HLA-DQB1*02 carrier frequency was 94.94%,meaning that only 5.06%of CD patients were completely lacking this allelic variant.Interestingly,if we consider only the studies whereby the prevalence of CD patients affected with type 1 diabetes mellitus was supposed or clearly established to be very low,the frequency of non-HLA-DQB1*02 carriers among CD patients dropped to 3.65%.CONCLUSION Such a high carrier frequency of the HLA-DQB1*02 allelic variant(which is>95%-96%in CD patients without risk factors,like type 1 diabetes mellitus comorbidity)might be exploited to consider a cost-effective and widened screening approach.If a sustainable strategy could be implemented through a low-cost targeted genetic test to detect the individual presence of HLA-DQB1*02 allele,an appropriate algorithm for serological screening in individuals resulting to be genetically predisposed to CD,might be considered.
文摘目的从基因水平探讨湖北地区汉族人食管癌 HEN-DQB1等位基因的遗传易感性.方法运用序列特异性引物聚合酶链反应技术,检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的 HLA-DQB1等位基因.SAS system 统计软件数据处理.结果湖北汉族人食管癌患者与正常人比较,HEN-DQB1*0301基因频率显著增高(0.2976 vs 0.1875),P=0.046,OR=1.835,病因分数=0.1354);两组间 HLA-DQB1其余各等位基因分布频率的比较,HLA-DQB1*0201(0.0833 vs 0.1016),*0301(0.2976 vs 0.1875),*0302(0.0595 vs 0859),*0303(0.2381 vs 0.1875),*0304(0.0000 vs 0.0039),*0401(0.0714 vs 0.0469),*0402(0.0119 vs 0.0156),*0501(0.0357 vs 0.0703),*0502(0.0595 vs 0.0664),*0503(0.0119 vs 0.0195),*0504(0.0000 vs 0.0039),*0601(0.0595 vs 0.0781),*0602(0.0476 vs 0.0742),*0603(0.0000 vs 0.0078),*0604(0.0238 vs 0.0508),差异均无显著性.结论 HLA-DQB1*0301等位基因与湖北汉族人食管癌正关联,为其易感基因.
