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Prevalence and Factors Associated with Positivity of Antinuclear Antibodies (ANA) Patterns, Native Anti-DNA and Extractable Nuclear Antigens (ENA) Antibodies: Experience from a Laboratory in Dakar
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作者 Diop Abdou Diallo Thierno Abdoulaye +4 位作者 Ndiaye Babacar Mahou Chantal Diop Marième Gaye Dubrous Phillippe Seck Abdoulaye 《Open Journal of Rheumatology and Autoimmune Diseases》 2024年第1期26-36,共11页
Background: Diagnosis of autoimmune diseases (AID) is challenging, due to overlapping features with other non-immune disorders. Anti-nuclear antibodies (ANA) are sensitive screening tests but anti-deoxyribonucleic aci... Background: Diagnosis of autoimmune diseases (AID) is challenging, due to overlapping features with other non-immune disorders. Anti-nuclear antibodies (ANA) are sensitive screening tests but anti-deoxyribonucleic acid-antibody (anti-DNA), and anti-extractable nuclear antigens (anti-ENA) are specific for AIDs. We aimed to look at ANA patterns in our patients and correlated them with anti-ENA for proper interpretation and better patient management cost-effectively. Methods: A retrospective study was conducted over 1 year from January to December 2022 who were tested for ANA at biology medical laboratory of Pasteur Institute of Dakar. Anti-ENA and anti-DNA results were also analyzed for ANA-positive patients. Statistical analysis was performed using STATA 14.0, p Results: 216 patients were analyzed. Women predominated at 79.2% and mean age was 48 years [CI 95%, 46 - 50], with extremes of 10 and 89. Most represented age group was [41 - 60] with 38%. ANA was positive in 27 (12.5%) of patients, 59.2% of whom were strongly positive (titer of 1/1000, 1/3200 or 1/6400). The most common pattern was nuclear speckled, which was found in 77.8% of samples. Anti-ENA and anti-DNA positivity in ANA-positive patients was found respectively in 63% (17/27) and 1.4% (3/27) of the samples analyzed. Most commonly identified anti-ENA was anti-Sm 29.6%, anti-SSA 29.6%, anti-Ro-52 25.9%, anti-RNP 18.5% and anti-SSB 14.8% which was associated with speckled pattern. Association results indicated a significant relationship between both tests and between ANA titer in the anti-ENA- and ANA-positive patients (p 0.001). Conclusions: ANA, Anti-ENA and anti-DNA antibodies are essential for AIDS diagnosis. However, the testing repertoire should follow an algorithm comprising of clinical features, followed by ANA results with nuclear, mitotic, and cytoplasmic patterns, anti-ENA, and anti-DNA for a more meaningful, and cost-effective diagnostic approach. 展开更多
关键词 Antinuclear Antibodies Extractable Nuclear antigen Autoimmune Disease Indirect Immunofluorescence
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Inflammatory response in gastrointestinal cancers:Overview of six transmembrane epithelial antigens of the prostate in pathophysiology and clinical implications
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作者 Ze-Xuan Fang Wen-Jia Chen +4 位作者 Zheng Wu Yan-Yu Hou Yang-Zheng Lan Hua-Tao Wu Jing Liu 《World Journal of Clinical Oncology》 2024年第1期9-22,共14页
Chronic inflammation is known to increase the risk of gastrointestinal cancers(GICs),the common solid tumors worldwide.Precancerous lesions,such as chronic atrophic inflammation and ulcers,are related to inflammatory ... Chronic inflammation is known to increase the risk of gastrointestinal cancers(GICs),the common solid tumors worldwide.Precancerous lesions,such as chronic atrophic inflammation and ulcers,are related to inflammatory responses in vivo and likely to occur in hyperplasia and tumorigenesis.Unfortunately,due to the lack of effective therapeutic targets,the prognosis of patients with GICs is still unsatisfactory.Interestingly,it is found that six transmembrane epithelial antigens of the prostate(STEAPs),a group of metal reductases,are significantly associated with the progression of malignancies,playing a crucial role in systemic metabolic homeostasis and inflammatory responses.The structure and functions of STEAPs suggest that they are closely related to intracellular oxidative stress,responding to inflammatory reactions.Under the imbalance status of abnormal oxidative stress,STEAP members are involved in cell transformation and the development of GICs by inhibiting or activating inflammatory process.This review focuses on STEAPs in GICs along with exploring their potential molecular regulatory mechanisms,with an aim to provide a theoretical basis for diagnosis and treatment strategies for patients suffering from these types of cancers. 展开更多
关键词 Six transmembrane epithelial antigens of the prostate Gastrointestinal cancer Inflammation
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Six transmembrane epithelial antigens of the prostate to illustrate inflammatory response in gastrointestinal cancers
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作者 Yi-Han Wu Lian-Xiang Luo 《World Journal of Clinical Oncology》 2024年第8期961-964,共4页
Gastrointestinal cancer(GIC)is a common and widespread form of tumor,with colonoscopy and upper gastrointestinal endoscopy available to detect relevant precancerous polyps and lesions.However,many patients are already... Gastrointestinal cancer(GIC)is a common and widespread form of tumor,with colonoscopy and upper gastrointestinal endoscopy available to detect relevant precancerous polyps and lesions.However,many patients are already in the late stages when first diagnosed with such cancer,resulting in a poor prognosis.Thus,it is necessary to explore new methods and research directions in order to improve the treatment of GIC.Given the specific nature of the gastrointestinal tract,research should focus on the mechanisms of various inflammations and the interactions between food entering and exiting from the gastrointestinal tract and cancer cells.Interestingly,six transmembrane epithelial antigens of the prostates(STEAPs)have been found to be significantly linked to the progression of malignant tumors,associated with intracellular oxidative stress and playing a major role in inflammation with their structure and function.This paper explores the mechanism of STEAPs in the inflammatory response of GIC,providing a theoretical basis for the prevention and early intervention of GIC.The basic properties of the STEAP family as metal reductase are also explained.When it comes to intervention for GIC prevention,STEAPs can affect the activity of Fe3+,Cu2+reductase and regulate metal ion uptake in vivo,participating in inflammation-related iron and copper homeostasis.Thus,the mechanism of STEAPs on inflammation is of important value in the prevention of GIC. 展开更多
关键词 Six transmembrane epithelial antigens of the prostate Gastrointestinal cancer Inflammation Gastric cancer Colorectal cancer Hepatocellular carcinoma
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Tumor neoantigens: Novel strategies for application of cancer immunotherapy 被引量:3
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作者 HANYANG GUAN YUE WU +10 位作者 LU LI YABING YANG SHENGHUI QIU ZHAN ZHAO XIAODONG CHU JIASHUAI HE ZUYANG CHEN YIRAN ZHANG HUI DING JINGHUA PAN YUNLONG PAN 《Oncology Research》 SCIE 2023年第4期437-448,共12页
Neoantigen-targeted immunotherapy is a rapidly advancing field that holds great promise for treating cancer.The recognition of antigens by immune cells is a crucial step in tumor-specific killing,and neoantigens gener... Neoantigen-targeted immunotherapy is a rapidly advancing field that holds great promise for treating cancer.The recognition of antigens by immune cells is a crucial step in tumor-specific killing,and neoantigens generated by mutations in cancer cells possess high immunogenicity and are selectively expressed in tumor cells,making them an attractive therapeutic target.Currently,neoantigens find utility in various domains,primarily in the realm of neoantigen vaccines such as DC vaccines,nucleic acid vaccines,and synthetic long peptide vaccines.Additionally,they hold promise in adoptive cell therapy,encompassing tumor-infiltrating cells,T cell receptors,and chimeric antigen receptors which are expressed by genetically modified T cells.In this review,we summarized recent progress in the clinical use of tumor vaccines and adoptive cell therapy targeting neoantigens,discussed the potential of neoantigen burden as an immune checkpoint in clinical settings.With the aid of state-of-the-art sequencing and bioinformatics technologies,together with significant advancements in artificial intelligence,we anticipated that neoantigens will be fully exploited for personalized tumor immunotherapy,from screening to clinical application. 展开更多
关键词 IMMUNOTHERAPY Tumor vaccine Adoptive T cell therapy Chimeric antigen receptor
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Expression and Evaluation of Wb-SXP-1 and Wb-123 Recombinant Antigens as Potential Diagnostic Biomarkers for Lymphatic Filariasis
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作者 Sharlene Kerubo Mageto Rebecca Wanjiku Waihenya +11 位作者 Anne Wanjiru Mwangi Peter Kipkemboi Rotich Matthew Mutinda Munyao Tonny Teya Robinson Mugasiali Irekwa Joanne Jepkemei Yego Caroline Wangui Njoroge Grace Ng’endo Kanyita Nicole Sian Tanchu Dawala Koromtili Oumar Primrose Muthoni Ndungu Samson Muuo Nzou 《American Journal of Molecular Biology》 CAS 2023年第2期95-112,共18页
Lymphatic filariasis (LF) remains a public health concern as it can cause permanent morbidity and disability to those infected. While the global elimination of LF in these endemic areas is ongoing through mass drug ad... Lymphatic filariasis (LF) remains a public health concern as it can cause permanent morbidity and disability to those infected. While the global elimination of LF in these endemic areas is ongoing through mass drug administration, there is the need to develop diagnostic tools that would be utilized to track the progress of total global eradication as well as perform surveillance for the recurrence of lymphatic filariasis transmission. Currently, approved LF diagnosis tools are faced with lack of specificity, low sensitivity, and periodicity dependence. Recombinant filarial antigen-based assays can address these drawbacks and offer practical instruments for LF diagnosis and surveillance. This present study, evaluated rWb-SXP-1 and rWb-123 antigens as potential diagnostic biomarker tools for Wuchereria banchrofti in human sera using microspheres-based multiplex serological assay. Based on statistical analysis using XLSTAT 2019 (Addinsoft) on data generated from multiplex technology assay, generated ROC curves for both rWb-SXP-1 and rWb-123 demonstrated 87.1% sensitivity to Wuchereria banchrofti human sera with rWb-SXP-1 antigens having the highest specificity of 96%. Indication that rWb-SXP-1 and rWb-123 antigens are capable of detecting immunoglobulin G4 (IgG4) antibodies in human sera synthesized specifically against W. banchrofti infections. Therefore, rWb-SXP-1 and rWb-123 antigens can be utilized to detect W. banchrofti infections by antibody profiling with excellent diagnostic sensitivity and specificity using microsphere-based multiplex serological tests. This method can be particularly practical for screening a large number of sera samples and/or for quick, extensive field-testing due to the high-throughput and quick formats applied. 展开更多
关键词 Lymphatic Filariasis Recombinant antigens DIAGNOSIS
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Identification of tumor antigens and immune subtypes of hepatocellular carcinoma for mRNA vaccine development
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作者 Tai-Liang Lu Cheng-Long Li +2 位作者 Yong-Qiang Gong Fu-Tao Hou Chao-Wu Chen 《World Journal of Gastrointestinal Oncology》 SCIE 2023年第10期1717-1738,共22页
BACKGROUND mRNA vaccines have been investigated in multiple tumors,but limited studies have been conducted on their use for hepatocellular carcinoma(HCC).AIM To identify candidate mRNA vaccine antigens for HCC and sui... BACKGROUND mRNA vaccines have been investigated in multiple tumors,but limited studies have been conducted on their use for hepatocellular carcinoma(HCC).AIM To identify candidate mRNA vaccine antigens for HCC and suitable subpopu-lations for mRNA vaccination.METHODS Gene expression profiles and clinical information of HCC datasets were obtained from International Cancer Genome Consortium and The Cancer Genome Atlas.Genes with somatic mutations and copy number variations were identified by cBioPortal analysis.The differentially expressed genes with significant prognostic value were identified by Gene Expression Profiling Interactive Analysis 2 website analysis.The Tumor Immune Estimation Resource database was used to assess the correlation between candidate antigens and the abundance of antigen-presenting cells(APCs).Tumor-associated antigens were overexpressed in tumors and associated with prognosis,genomic alterations,and APC infiltration.A consensus cluster analysis was performed with the Consensus Cluster Plus package to identify the immune subtypes.The weighted gene coexpression network analysis(WGCNA)was used to determine the candidate biomarker molecules for appropriate populations for mRNA vaccines.immune subtypes showed distinct cellular and clinical characteristics.The IS1 and IS3 immune subtypes were immunologically“cold”.The IS2 and IS4 immune subtypes were immunologically“hot”,and the immune checkpoint genes and immunogenic cell death genes were upregulated in these subtypes.IS1-related modules were identified with the WGCNA algorithm.Ultimately,five hub genes(RBP4,KNG1,METTL7A,F12,and ABAT)were identified,and they might be potential biomarkers for mRNA vaccines.CONCLUSION AURKA,CCNB1,CDC25C,CDK1,TRIP13,PES1,MCM3,PPM1G,NEK2,KIF2C,PTTG1,KPNA2,and PRC1 have been identified as candidate HCC antigens for mRNA vaccine development.The IS1 and IS3 immune subtypes are suitable populations for mRNA vaccination.RBP4,KNG1,METTL7A,F12,and ABAT are potential biomarkers for mRNA vaccines. 展开更多
关键词 mRNA vaccine Hepatocellular carcinoma Immunotype antigens Immune subtypes
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False positive detection of serum cryptococcal antigens due to insufficient sample dilution:A case series
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作者 Wen-Yu Chen Cheng Zhong +1 位作者 Jian-Ying Zhou Hua Zhou 《World Journal of Clinical Cases》 SCIE 2023年第8期1837-1846,共10页
At present,with the development of technology,the detection of cryptococcal antigen(CRAG)plays an increasingly important role in the diagnosis of cryptococcosis.However,the three major CRAG detection technologies,late... At present,with the development of technology,the detection of cryptococcal antigen(CRAG)plays an increasingly important role in the diagnosis of cryptococcosis.However,the three major CRAG detection technologies,latex agglutination test(LA),lateral flow assay(LFA)and Enzyme-linked Immunosorbent Assay,have certain limitations.Although these techniques do not often lead to false-positive results,once this result occurs in a particular group of patients(such as human immunodeficiency virus patients),it might lead to severe consequences. 展开更多
关键词 CRYPTOCOCCOSIS Capsular antigen detection False positive TISSUE Case report
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HLA-G14bp插入/缺失多态性与1型糖尿病的相关性研究
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作者 章康威 谢玉霜 +4 位作者 付晓俐 潘欣怡 吴志农 单士刚 包永芬 《湖北科技学院学报(医学版)》 2024年第5期405-408,共4页
目的探讨湖北汉族糖尿病患者HLA-G14bp插入/缺失多态性与1型糖尿病易感性的相关性。方法采用聚合酶链反应-序列特异引物(PCR-SSP)方法,对湖北地区70例汉族1型糖尿病患者和100例正常对照者进行HLA-G14bp基因的插入/缺失位点检测,并对各... 目的探讨湖北汉族糖尿病患者HLA-G14bp插入/缺失多态性与1型糖尿病易感性的相关性。方法采用聚合酶链反应-序列特异引物(PCR-SSP)方法,对湖北地区70例汉族1型糖尿病患者和100例正常对照者进行HLA-G14bp基因的插入/缺失位点检测,并对各基因型与临床症状的相关性进行分析。结果HLA-G14bp插入/缺失多态性在健康者和1型糖尿病患者均显示Hardy-Weinberg平衡;1型糖尿病患者与健康对照组比较,HLA-G14bp+/+、+/-及-/-基因型存在显著性差异(P<0.05);HLA-G14bp的插入/缺失位点在不同的病程中无显著性差异(P>0.05)。结论在湖北地区汉族人群中,HLA-G14bp插入/缺失多态性可能与1型糖尿病疾病易感性相关。 展开更多
关键词 1型糖尿病 hla-g14bp 插入/缺失 多态性
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HLA-G 3′UTR基因多态性及血清可溶性HLA-G水平与儿童诺如病毒感染的相关性研究
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作者 朱明武 李多多 +1 位作者 刘向群 王亚南 《检验医学与临床》 CAS 2024年第8期1041-1045,共5页
目的探讨人类白细胞抗原-G(HLA-G)3′非翻译区(3′UTR)基因多态性(SNP)及血清可溶性HLA-G(sHLA-G)水平与儿童诺如病毒感染的相关性。方法选取2021年1月至2022年12月新乡医学院第一附属医院收治的346例诺如病毒感染患儿作为病例组,另选... 目的探讨人类白细胞抗原-G(HLA-G)3′非翻译区(3′UTR)基因多态性(SNP)及血清可溶性HLA-G(sHLA-G)水平与儿童诺如病毒感染的相关性。方法选取2021年1月至2022年12月新乡医学院第一附属医院收治的346例诺如病毒感染患儿作为病例组,另选取同期320例健康体检儿童作为对照组,留取所有研究对象全血标本并提取DNA。采用聚合酶链反应扩增HLA-G 3′UTR基因,产物外送测序。使用SNPstats在线分析软件计算两组间SNP位点基因型和等位基因分布频率。采用酶联免疫吸附试验检测病例组和对照组血清sHLA-G水平。结果病例组和对照组HLA-G 3′UTR 14 bp+/-和+3142 C/G基因型和等位基因分布频率比较,差异均无统计学意义(P>0.05)。病例组血清sHLA-G水平明显高于对照组,差异有统计学意义(P<0.05);诺如病毒不同程度感染组血清sHLA-G水平比较,差异均无统计学意义(P>0.05)。病例组和对照组14 bp+/-基因型和+3142 C/G基因型对应的血清sHLA-G水平比较,差异均无统计学意义(P>0.05)。结论HLA-G 3′UTR SNP与诺如病毒感染的易感性无关。血清sHLA-G水平对诺如病毒感染早期诊断有较好的预测价值,可能是一种新的血清生物标志物,与患者病情严重程度及预后的关联尚需进一步验证。 展开更多
关键词 诺如病毒 人类白细胞抗原-G 可溶性人类白细胞抗原-G 基因多态性 免疫调节 相关性
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人类白细胞抗原G(HLA-G)与非小细胞肺癌的研究进展
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作者 李蕊 郭宗伟 +1 位作者 张效云 肖漓 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2024年第2期174-178,共5页
人类白细胞抗原G(HLA-G)属于非经典主要组织相容性复合体Ⅰb(MHCⅠb)类分子,包括膜结合型和可溶性HLA-G两种形式,通过相应受体调节多种免疫细胞的功能,是形成母胎耐受、肿瘤免疫逃逸的重要免疫学机制之一。非小细胞肺癌(NSCLC)在肺癌中... 人类白细胞抗原G(HLA-G)属于非经典主要组织相容性复合体Ⅰb(MHCⅠb)类分子,包括膜结合型和可溶性HLA-G两种形式,通过相应受体调节多种免疫细胞的功能,是形成母胎耐受、肿瘤免疫逃逸的重要免疫学机制之一。非小细胞肺癌(NSCLC)在肺癌中占比最高且预后差,研究发现HLA-G的基因多态性及表达水平与NSCLC发生发展密切相关,提示HLA-G可作为NSCLC早期诊断、亚型区分、治疗及预后等潜在的生物标志物,具有辅助诊断依据的临床价值,对其机制的深入研究更可能提供NSCLC诊疗的新策略。 展开更多
关键词 人类白细胞抗原G(hla-g) hla-g多态性 非小细胞肺癌(NSCLC) 免疫逃逸 生物标志物 综述
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Tumor-Specific Histo-Blood Group Antigens: Apropos of Two Cases
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作者 Ferenc Baranyay 《Case Reports in Clinical Medicine》 2023年第7期232-250,共19页
Cancer cells with immunogenic properties having altered protein glycosylation, modified blood group substances have been widely studied. Due to the genetic instability occurring during carcinogenesis the glycosyltrans... Cancer cells with immunogenic properties having altered protein glycosylation, modified blood group substances have been widely studied. Due to the genetic instability occurring during carcinogenesis the glycosyltransferases may suffer from posttranslation sequence modification. The author describes 2 autopsy cases, where in the background of the unusual metastatic tumor presentation, incompatible blood group antigenic determinants have been demonstrated using blood group specific lectins and monoclonal antibodies (mAb). In the first case, reported here, a 10-year-old girl developed an acute myeloid leukemia and died in a septic endotoxin shock after successful cytostatic treatment of a juvenile signet ring cell cancer of her colon. At autopsy there were no signs of tumor except bilateral apple-sized mucinous ovarian (Krukenberg) metastases. While she had erythrocyte phenotype of blood group A, the signet ring adenocarcinoma cells expressed blood group B incompatible antigenic determinants with lectin/mAb. In the second case, the autopsy of a 78-year-old female resulted in no macroscopic tumor sign except a moderately enlarged, ham hard spleen. Light microscopy revealed adenocarcinomatous infiltration in the splenic sinusoids. The patient had blood group O, while the metastatic cells in the spleen reacted with Breast Carcinoma Antigen (BioGenex) and incompatible anti-B Banderiaeasimplicifolia agglutinin I and anti-B mAb. It proved to be a case of an occult, completely regressed breast cancer. Based on these observations the expression of tumor specific incompatible blood group antigens might occur from time to time, mostly in adenocarcinomas. Accordingly, blood group-based specific immuno-oncotherapy could be considered in some cancer cases. 展开更多
关键词 Occult Breast Cancer Krukenberg Metastasis Incompatible Tumor-Specific Histo-Blood Group antigens
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Three-dimensional models of antigens with serodiagnostic potential for leprosy:An in silico study
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作者 Bianca Luiza Melo de Assis Rafaela Viana Vieira +4 位作者 Ian Theodoro Rudenco Gomes Palma Matheus Bertolini Coutinho Juliana de Moura Gabrielle Caroline Peiter Kádima Nayara Teixeira 《World Journal of Clinical Infectious Diseases》 2023年第1期1-10,共10页
BACKGROUND Leprosy is a disease caused by Mycobacterium leprae(M.leprae),an intracellular pathogen that has tropism and affects skin and nervous system cells.The disease has two forms of presentation:Paucibacillary an... BACKGROUND Leprosy is a disease caused by Mycobacterium leprae(M.leprae),an intracellular pathogen that has tropism and affects skin and nervous system cells.The disease has two forms of presentation:Paucibacillary and multibacillary,with different clinical and immunological manifestations.Unlike what occurs in the multibacillary form,the diagnostic tests for the paucibacillary form are nonspecific and not very sensitive,allowing the existence of infected individuals without treatment,which contributes to the spread of the pathogen in the population.To mitigate this contamination,more sensitive diagnostic tests capable of detecting paucibacillary patients are needed.AIM To predict the three-dimensional structure models of M.leprae antigens with serodiagnostic potential for leprosy.METHODS In this in silico study,satisfactory templates were selected in the Protein Data Bank(PDB)using Basic Local Alignment Search Tool to predict the structural templates of ML2038,ML0286,ML0050,and 85B antigens by comparative modeling.The templates were selected according to general criteria such as sequence identity,coverage,X-ray resolution,Global Model Quality Estimate value and phylogenetic relationship;Clustal X 2.1 software was used in this analysis.Molecular modeling was completed using the software Modeller 9v13.Visualization of the models was made using ViewerLite 4.2 and PyMol software,and analysis of the quality of the predicted models was performed using the QMEAN score and Z-score.Finally,the three-dimensional moels were validated using the MolProbity and Verify 3D platforms.RESULTS The three-dimensional structure models of ML2038,ML0286,ML0050,and 85B antigens of M.leprae were predicted using the templates PDB:3UOI(90.51%identity),PDB:3EKL(87.46%identity),PDB:3FAV(40.00%identity),and PDB:1F0N(85.21%identity),respectively.The QMEAN and Z-score values indicated the good quality of the structure models.These data refer to the monomeric units of antigens,since some of these antigens have quaternary structure.The validation of the models was performed with the final three-dimensional structure-monomer(ML0050 and 85B antigens)and quaternary structures(ML2038 and ML0286).The majority of amino acid residues were observed in favorable and allowed regions in the Ramachandran plot,indicating correct positioning of the side chain and absence of steric impediment.The MolProbity score value and Verify 3D results of all models indicated a satisfactory prediction.CONCLUSION The polarized immune response against M.leprae creates a problem in leprosy detection.The selection of immunodominant epitopes is essential for the development of more sensitive serodiagnostic tests,for this it is important to know the three-dimensional structure of the antigens,which can be predicted with bioinformatics tools. 展开更多
关键词 antigens Leprosy diagnosis Mycobacterium leprae Molecular modelling Serological test In silico study
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HPV感染相关CIN患者血清HLA-E和HLA-G表达与疾病进展及治疗后复发的相关性 被引量:2
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作者 魏艳飞 徐春燕 +3 位作者 魏玲 刘勃 于倩 董闯 《安徽医学》 2024年第1期34-38,共5页
目的探讨人乳头瘤病毒(HPV)感染相关宫颈上皮内瘤变(CIN)患者血清人类白细胞抗原E(HLA-E)、人类白细胞抗原G(HLA-G)表达及临床意义。方法选取2021年6~12月在河北中石油中心医院治疗的HPV感染相关CIN患者120例作为观察组,选取同期无HPV... 目的探讨人乳头瘤病毒(HPV)感染相关宫颈上皮内瘤变(CIN)患者血清人类白细胞抗原E(HLA-E)、人类白细胞抗原G(HLA-G)表达及临床意义。方法选取2021年6~12月在河北中石油中心医院治疗的HPV感染相关CIN患者120例作为观察组,选取同期无HPV感染的CIN患者60例作为对照组,检测两组血清HLA-E和HLA-G表达水平,分析血清HLA-E和HLA-G表达与患者临床资料、治疗后复发的关系。结果观察组血清HLA-E和HLA-G水平分别为(75.59±18.28)pg/mL和(30.20±9.92)ng/mL,高于对照组,差异有统计学意义(P<0.05)。观察组CIN分级Ⅱ/Ⅲ级患者血清HLA-E和HLA-G分别为(83.33±13.90)pg/mL和(34.54±7.18)ng/mL,高于CIN分级Ⅰ级(P<0.05);观察组高危型HPV感染者血清HLA-E和HLA-G分别为(78.82±17.70)pg/mL和(32.23±9.43)ng/mL,高于低危型HPV感染者(P<0.05)。观察组血清HLA-E和HLA-G表达与CIN分级呈正相关(P<0.05)。复发患者治疗后1周血清HLA-E和HLA-G分别为(68.32±8.73)pg/mL和(24.43±5.58)ng/mL,高于未复发患者(P<0.05)。结论HPV感染相关CIN患者血清HLA-E和HLA-G表达与CIN分级呈正相关,同时与患者治疗后复发有一定关系。 展开更多
关键词 人乳头瘤病毒 宫颈上皮内瘤变 人类白细胞抗原E 人类白细胞抗原G 治疗后复发
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Serum tumor markers (carcinoembryonic antigen, carbohydrate antigen 19-9, carbohydrate antigen 72-4, carbohydrate antigen 24-2, ferritin) and gastric cancer prognosis correlation
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作者 Jie-Wen Zhu Ling-Zhen Gong Qian-Wen Wang 《World Journal of Gastrointestinal Surgery》 SCIE 2024年第9期2808-2814,共7页
BACKGROUND Gastric cancer is a kind of malignant tumor which is prevalent all over the world.Although some progress has been made in the treatment of gastric cancer,its prognosis is still not optimistic,so it is of gr... BACKGROUND Gastric cancer is a kind of malignant tumor which is prevalent all over the world.Although some progress has been made in the treatment of gastric cancer,its prognosis is still not optimistic,so it is of great significance to find reliable prog-nostic indicators to guide the treatment and management of patients with gastric cancer.AIM To explore the relationship between serum levels of five biomarkers[carcinoem-bryonic antigen(CEA),carbohydrate antigen(CA)19-9,CA72-4,CA24-2,and ferritin]and prognosis in patients with gastric cancer.METHODS This study included 200 patients with gastric adenocarcinoma,and conducted an in-depth analysis of their baseline characteristics,relationship between tumor markers and staging,and prognosis.The study found that CA19-9 has a signi-ficant correlation with tumor stage,the average levels of CA24-2,CEA,CA72-4 and ferritin were slightly increased disregarding the stage of tumor.Survival analysis showed that increases in CEA,CA19-9,CA24-2,and ferritin were all associated with shortened overall survival of patients.Further multivariate ana-lysis revealed that elevated serum CA72-4 levels were an inde-pendent adverse prognostic factor.RESULTS This study reveals that there is a significant correlation between the expression levels of serum tumor markers CEA,CA19-9,CA72-4,CA24-2 and ferritin in patients with gastric cancer and prognosis,and can be used as important indicators for prognostic evaluation of gastric cancer.In particular,markers that appear abnormally elevated initially may help identify gastric cancer patients with poor prognosis.CONCLUSION Serum CEA and CA19-9 play an important role in the prognosis assessment of gastric cancer,and are effective tools to guide clinical practice and optimize individualized treatment strategies for gastric cancer patients. 展开更多
关键词 Gastric cancer PROGNOSIS Carcinoembryonic antigen Carbohydrate antigen 19-9 Carbohydrate antigen 72-4 Carbohydrate antigen 24-2 FERRITIN Serum markers Retrospective study
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The combined detection of carcinoembryonic antigen,carcinogenic antigen 125,and carcinogenic antigen 19-9 in colorectal cancer patients
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作者 Ling-Zhen Gong Qian-Wen Wang Jie-Wen Zhu 《World Journal of Gastrointestinal Surgery》 SCIE 2024年第7期2073-2079,共7页
BACKGROUND Hepatic metastases are common and difficult to treat after colorectal cancer(CRC)surgery.The predictive value of carcinoembryonic antigen(CEA),cancer antigen(CA)125 and CA19-9 combined tests for liver metas... BACKGROUND Hepatic metastases are common and difficult to treat after colorectal cancer(CRC)surgery.The predictive value of carcinoembryonic antigen(CEA),cancer antigen(CA)125 and CA19-9 combined tests for liver metastasis is unclear.AIM To evaluate predictive value of combined tests for CEA,CA125,and CA19-9 levels in patients with liver metastases of CRC.METHODS The retrospective study included patients with CRC alone(50 cases)and patients with CRC combined with liver metastases(50 cases)who were hospitalized between January 2021 and January 2023.Serum CEA,CA125 and CA19-9 levels were compared between the two groups,and binary logistic regression was used to analyze the predictive value of the combination of these tumor markers in liver metastasis.In addition,we performed receiver operating characteristic(ROC)curve analysis to assess its diagnostic accuracy.RESULTS The results showed that the serum CEA,CA125 and CA19-9 levels in the CRC with liver metastasis group were significantly higher than those in the CRC alone group.Specifically,the average serum CEA level in the CRC with liver metastasis group was 162.03±810.01 ng/mL,while that in the CRC alone group was 5.71±9.76 ng/mL;the average serum CA125 levels were 43.47±83.52 U/mL respectively.and 13.5±19.68 U/mL;the average serum CA19-9 levels were 184.46±473.13 U/mL and 26.55±43.96 U/mL respectively.In addition,binary logistic regression analysis showed that CA125 was significant in predicting CRC liver metastasis(P<0.05).ROC curve analysis results showed that the areas under the ROC curves of CEA,CA125 and CA19-9 were 0.607,0.692 and 0.586.CONCLUSION These results suggest that combined detection of these tumor markers may help early detection and intervention of CRC liver metastasis,thereby improving patient prognosis. 展开更多
关键词 Colorectal cancer Liver metastasis Serum markers Carcinoembryonic antigen Cancer antigen 125 Cancer antigen 19-9 Receiver operating characteristic analysis
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Prostate cancer with elevated free prostate-specific antigen density:A case report
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作者 Deng-Hui Huang Yun-Xi Hu +1 位作者 Shuang Guo Wen-Jiang Yang 《World Journal of Clinical Cases》 SCIE 2024年第17期3259-3264,共6页
BACKGROUND Prostate cancer is the second most common cancer among men worldwide,and prostate-specific antigen(PSA)is often used in clinical practice to screen for prostate cancer.Normal total PSA(tPSA)level initially ... BACKGROUND Prostate cancer is the second most common cancer among men worldwide,and prostate-specific antigen(PSA)is often used in clinical practice to screen for prostate cancer.Normal total PSA(tPSA)level initially excludes prostate cancer.Here,we report a case of prostate cancer with elevated free PSA density(fPSAD).CASE SUMMARY A patient diagnosed with benign prostatic hyperplasia underwent prostatectomy,and the postoperative pathological results showed acinar adenocarcinoma of the prostate.The patient is currently undergoing endocrine chemotherapy.CONCLUSION We provide a clinical reference for diagnosis and treatment of patients with normal tPSA but elevated fPSAD. 展开更多
关键词 Prostate cancer Free prostate-specific antigen density Total prostate-specific antigen Case report
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Engineering W/O/W pickering emulsion for the enhanced antigen delivery and immune responses
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作者 Sihua Wu Xiaonan Huang +2 位作者 Yin Ma Naoki Asakawa Yufei Xia 《Particuology》 SCIE EI CAS CSCD 2024年第9期224-233,共10页
Tumor immunotherapy,particularly cancer vaccines,holds promise for combating cancer by harnessing tailored immune responses against malignant cells.However,conventional approaches face challenges in efficiently delive... Tumor immunotherapy,particularly cancer vaccines,holds promise for combating cancer by harnessing tailored immune responses against malignant cells.However,conventional approaches face challenges in efficiently delivering antigens for optimal immune activation.Emulsion adjuvants,like MF59,enhance cellular uptake but struggle to induce robust CD8^(+)T cell responses.Here,we introduce a novel strategy employing a water-in-oil-in-water(W/O/W)multiple Pickering emulsion(mPE)for antigen delivery.The mPE,utilizing biocompatible,pH-sensitive particles,encapsulates antigens within the inner water phase,ensuring enhanced intracellular processing and dictating the intracellular fate of antigens for improved cross-presentation.In vitro and in vivo studies demonstrated that mPEs induced robust dendritic cells activation and antigen cross-presentation,leading to a significantly enhanced immune response.Notably,calcium phosphate-stabilized mPE(CaP-mPE)illustrated the more robust IFN-γ^(+)T cell responses.In comparison with traditional surfactant-stabilized multiple emulsions,CaP-mPE significantly inhibit tumor growth and effectively prolong the survival of tumor-bearing mice.This innovative approach offers a promising avenue for the development of effective cancer vaccines with potent cellular immune responses. 展开更多
关键词 Cancer vaccine Multiple pickeringemulsion ADJUVANT antigen delivery Anti-tumor response
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Human leukocyte antigen compatibility and incidence of donorspecific antibodies in pediatric liver transplant recipients
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作者 Melina U Melere Flavia H Feier +8 位作者 Jorge Neumann Antônio N Kalil Juliana de M Montagner Luiza S Nader Carolina S da Silva Marco Aurélio F Junior Gabriela P Coral Guilherme P Bobsin Cristina T Ferreira 《World Journal of Gastroenterology》 SCIE CAS 2024年第33期3837-3845,共9页
BACKGROUND Antibody-mediated rejection following liver transplantation(LT)has been increasingly recognized,particularly with respect to the emergence of de novo donor-specific antibodies(DSAs)and their impact on graft... BACKGROUND Antibody-mediated rejection following liver transplantation(LT)has been increasingly recognized,particularly with respect to the emergence of de novo donor-specific antibodies(DSAs)and their impact on graft longevity.While substantial evidence for adult populations exists,research focusing on pediatric LT outcomes remains limited.AIM To investigate the prevalence of human leukocyte antigen(HLA)mismatches and DSA and evaluate their association with rejection episodes after pediatric LT.METHODS A cohort of pediatric LT recipients underwent HLA testing at Santa Casa de Porto Alegre,Brazil,between December 2013 and December 2023.Only patients who survived for>30 days after LT with at least one DSA analysis were included.DSA classes I and II and cross-matches were analyzed.The presence of de novo DSA(dnDSA)was evaluated at least 3 months after LT using the Luminex®single antigen bead method,with a positive reaction threshold set at 1000 MFI.Rejection episodes were confirmed by liver biopsy.RESULTS Overall,67 transplanted children were analyzed;61 received grafts from living donors,85%of whom were related to recipients.Pre-transplant DSA(class I or II)was detected in 28.3%of patients,and dnDSA was detected in 48.4%.The median time to DSA detection after LT was 19.7[interquartile range(IQR):4.3-35.6]months.Biopsyproven rejection occurred in 13 patients at follow-up,with C4d positivity observed in 5/13 Liver biopsies.The median time to rejection was 7.8(IQR:5.7-12.8)months.The presence of dnDSA was significantly associated with rejection(36%vs 3%,P<0.001).The rejection-free survival rates at 12 and 24 months were 76%vs 100%and 58%vs 95%for patients with dnDSA anti-DQ vs those without,respectively.CONCLUSION Our findings highlight the importance of incorporating DSA assessment into pre-and post-transplantation protocols for pediatric LT recipients.Future implications may include immunosuppression minimization strategies based on this analysis in pediatric LT recipients. 展开更多
关键词 Human leukocyte antigens Donor-specific antibodies Liver transplantation PEDIATRIC REJECTION
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阴道微生态失衡联合血清HLA-G、HLA-E水平评估HSIL患者CKC术后HPV持续感染价值
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作者 郑晶晶 张凡 +2 位作者 秦晶晶 刘馨雨 辛德梅 《中国计划生育学杂志》 2024年第7期1658-1662,共5页
目的:探究阴道微生态失衡联合血清人类白细胞抗原-G(HLA-G)、人类白细胞抗原-E(HLA-E)水平对宫颈高级别上皮内瘤变(HSIL)患者冷刀锥切(CKC)术后人乳头瘤病毒(HPV)持续感染的预测价值。方法:回顾性收集2021年1月-2022年12月本院收治HSIL... 目的:探究阴道微生态失衡联合血清人类白细胞抗原-G(HLA-G)、人类白细胞抗原-E(HLA-E)水平对宫颈高级别上皮内瘤变(HSIL)患者冷刀锥切(CKC)术后人乳头瘤病毒(HPV)持续感染的预测价值。方法:回顾性收集2021年1月-2022年12月本院收治HSIL患者137例临床资料,均行CKC治疗并术后随访1年,观察术后HPV持续感染情况,测定阴道微生态及血清HLA-G、HLA-E,分析阴道微生态及血清HLA-G、HLA-E水平与HPV持续感染关系以及评估价值。结果:137例术后1年内HPV持续感染31例(阳性组),HPV持续感染率为22.6%,未持续感染106例(阴性组),两组阴道pH值,阴道菌群密集度、多样性及滴虫阴道炎发生等均有差异,阳性组阴道微生态失衡率高于阴性组,血清HLA-G(19.84±3.53 ng/ml)、HLA-E(75.39±15.83 pg/ml)水平均高于阴性组(16.93±2.96 ng/ml、68.42±12.51 pg/ml),阳性组中阴道微生态失衡者血清HLA-G、HLA-E水平高于阴道微生态正常者(均P<0.05);Spearman相关分析显示,阴道微生态失衡、血清HLA-G、HLA-E水平与术后HPV持续感染呈正相关(r=0.633、0.529、0.608,均P<0.05);受试者工作特征曲线分析显示,阴道微生态失衡、血清HLA-G、HLA-E水平及联合评估术后HPV持续感染的曲线下面积分别为0.601、0.773、0.805、0.885(P<0.05)。结论:阴道微生态失衡联合血清HLA-G、HLA-E水平有对HSIL患者CKC术后HPV持续感染有一定预测价值。 展开更多
关键词 宫颈高级别上皮内瘤变 冷刀锥切 人乳头瘤病毒 阴道微生态失衡 人类白细胞抗原-G 人类白细胞抗原-E 持续感染 预测
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Technetium-99m-methylene diphosphonate single photon emission computed tomography/computed tomography combined with prostate-specific antigen/free prostate-specific antigen ratio for bone metastasis of prostate cancer
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作者 Jian He Yi-Pu Zhong Shuang Zhang 《World Journal of Clinical Cases》 SCIE 2024年第20期4082-4090,共9页
BACKGROUND Prostate cancer(PC)is one of the most common malignant tumors in men,and bone metastasis is one of its common complications,which seriously affects the quality of life and prognosis of patients.AIM To inves... BACKGROUND Prostate cancer(PC)is one of the most common malignant tumors in men,and bone metastasis is one of its common complications,which seriously affects the quality of life and prognosis of patients.AIM To investigate the diagnostic value of technetium-99m-methylene diphosphonate(99mTc-MDP)single photon emission computed tomography(SPECT)/CT imaging combined with the serum prostate-specific antigen(PSA)/free PSA ratio for PC bone metastasis(PCBM).METHODS One hundred patients with PC who visited the Hospital of Chengdu University of Traditional Chinese Medicine from January 2020 to January 2022 were recruited as the experimental(Exp)group,while 30 patients with benign prostatic lesions(BPLs)were recruited as the control(Ctrl)group.All patients underwent 99mTc-MDP SPECT/CT imaging and serum PSA/fPSA testing.The SPECT/CT imaging results and serum PSA/fPSA ratios of patients were analyzed to evaluate their diagnostic values for PCBM.RESULTS The difference in general information of the patients was not obvious,showing comparability.The two methods showed no visible differences in negative predictive value and sensitivity for patients with PCBM,but had great differences in positive predictive value and specificity(P<0.05).The PSA/fPSA ratio of patients with PC in the Exp group was lower than those with BPLs,and patients with PCBM had a much lower PSA/fPSA ratio than those without PC(P<0.05).The results confirmed that the combined use of 99mTc-MDP SPECT/CT imaging and serum PSA/fPSA ratio achieved a detection rate of 95%for PCBM.CONCLUSION The combination of 99mTc-MDP SPECT/CT and PSA/fPSA ratio is accurate and reliable for the diagnosis of PCBM,which provides an important reference for clinical practice. 展开更多
关键词 Matrix metalloproteinase 9 Single photon emission computed tomography Prostate-specific antigen to free prostate-specific antigen ratio Prostate cancer Bone metastasis
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