Objective:Circular ribose nudeic acids(circRNAs)are implicated in tumor progression and drug resistance of prostate cancer(PCa).The current work explored the function of circ_0005203(aircTHSD4)in the malignancy and do...Objective:Circular ribose nudeic acids(circRNAs)are implicated in tumor progression and drug resistance of prostate cancer(PCa).The current work explored the function of circ_0005203(aircTHSD4)in the malignancy and docetaxel(DTX)resistance of PCa.Methods:circTHSD4 expression within PCa as well as matched non-carcinoma samples was measured through real time reverse transcription quantitative polymerase chain reaction(RT-qPCR).In addition,a subcellular fraction assay was conducted to determine circTHSD4 subcellular localization within PCa cells.In addition,we performed a Western blot(WB)assay to detect high mobility.group A2 protein(HMGA2)levels.Besides,functional associations of two molecules were investigated through dual luciferase reporter assay.Cell Counting Kit(CCK)-8,colony formation together with Transwell assay was conducted to assess malignant phenotypes of PCa cells,whereas flow cytometry was performed to determine cell apoptosis.Furthermore,a xenograft mouse model was constructed to verify the effect of circTHSD4 on the carcinogenesis of PCa cells.Results:According to RT-qPCR results,circTHSD4 was up-regulated within PCa tissues and cells,which predicted the dismal prognostic outcome of PCa cases.circTHSD4 silencing within PCa cells markedly suppressed cell growth,migration,and colony fomation.circTHSD4 silencing remarkably elevated PCa cell apoptosis and carcinogenesis within the xenograft model.Further,circTHSD4 silencing enhanced docetaxel(DTX)sensitivity in PCa cells.Furthermore,we demonstrated that circTHSD4 modulated the malignancy of PCa cells by regulating HMGA2 expression through sponging miR 203.Conclusion:Together,our findings suggest that cirCTHSD4 overexpression could promote the malignant phenotype and DTX resistance in PCa through the regulation of the miR 203/HMGA2 axis.展开更多
In the article“Long non-coding RNA LINC02163 accelerates malignant tumor behaviors in breast cancer by regulating the microRNA-511-3p/HMGA2 axis as a competing endogenous RNA”(Oncology Research,2020,Vol.28,No.5,pp....In the article“Long non-coding RNA LINC02163 accelerates malignant tumor behaviors in breast cancer by regulating the microRNA-511-3p/HMGA2 axis as a competing endogenous RNA”(Oncology Research,2020,Vol.28,No.5,pp.483–495.doi:10.3727/096504020X15928179818438),there was an error in the processing of data.To further confirm our observation,we repeated multiple experiments involving in this study,including Flow Cytometry,Transwell Cell Migration and Invasion Assays,Xenograft Tumor Model,and Western Blotting.We have revised the figures to correct these errors.Corrected versions of the Figs.2,4,5,6,and 7 are provided.The corrections do not change any results or conclusion of the article.We apologize for any inconvenience caused.展开更多
BACKGROUND Gastric cancer(GC)is a common malignant tumor,long non-coding RNA and microRNA(miRNA)are important regulators that affect tumor proliferation,metastasis and chemotherapy resistance,and thus participate in t...BACKGROUND Gastric cancer(GC)is a common malignant tumor,long non-coding RNA and microRNA(miRNA)are important regulators that affect tumor proliferation,metastasis and chemotherapy resistance,and thus participate in tumor progression.CASC19 is a new bio-marker which can promote tumor invasion and metastasis.However,the mechanism by which CASC19 affects the progression of GC through miRNA is not clear.AIM To explore the role of the CASC19/miR-491-5p/HMGA2 regulatory axis in GC.METHODS To explore the expression and prognosis of CASC19 in GC through clinical samples,and investigate the effects of inhibiting CASC19 on the proliferation,migration,invasion and other functions of GC cells through cell counting Kit-8(CCK-8),ethynyldeoxyuridine,Wound healing assay,Transwell,Western blot and flow cytometry experiments.The effect of miR-491-5p and HMGA2 in GC were also proved.The regulatory relationship between CASC19 and miR-491-5p,miR-491-5p and HMGA2 were validated through Dual-luciferase reporter gene assay and reverse transcription PCR.Then CCK-8,Transwell,Wound healing assay,flow cytometry and animal experiments verify the role of CASC19/miR-491-5p/HMGA2 regulatory axis.RESULTS The expression level of CASC19 is related to the T stage,N stage,and tumor size of patients.Knockdown of the expression of CASC19 can inhibit the ability of proliferation,migration,invasion and EMT conversion of GC cells,and knocking down the expression of CASC19 can promote the apoptosis of GC cells.Increasing the expression of miR-491-5p can inhibit the proliferation of GC cells,miR-491-5p mimics can inhibit EMT conversion,and promote the apoptosis of GC cells,while decreasing the expression of miR-491-5p can promote the proliferation and EMT conversion and inhibit the apoptosis of GC cells.The expression of HMGA2 in GC tissues is higher than that in adjacent tissues.At the same time,the expression level of HMGA2 is related to the N and T stages of the patients.Reducing the level of HMGA2 can promote cell apoptosis and inhibit the proliferation of GC cells.Cell experiments and animal experiments have proved that CASC19 can regulates the expression of HMGA2 through miR-491-5p,thereby affecting the biological functions of GC.CONCLUSION CASC19 regulates the expression of HMGA2 through miR-491-5p to affect the development of GC.This axis may serve as a potential biomarker and therapeutic target of GC.展开更多
目的探讨胃癌患者外周血及组织中高迁移率族蛋白A1(high mobility group AT-hook1,HMGA1)表达及临床意义。方法应用酶联免疫吸附实验及免疫组织化学染色胃癌患者病理石腊包埋组织进行外周血及组织中HMGA1的表达检测;检索数据库分析HMGA...目的探讨胃癌患者外周血及组织中高迁移率族蛋白A1(high mobility group AT-hook1,HMGA1)表达及临床意义。方法应用酶联免疫吸附实验及免疫组织化学染色胃癌患者病理石腊包埋组织进行外周血及组织中HMGA1的表达检测;检索数据库分析HMGA1在胃癌肿瘤组织的表达以及总生存期;采用Western Blot及qRT-PCR检测胃癌肿瘤组织及癌旁组织中HMGA1蛋白及mRNA的表达。结果胃癌患者外周血中HMGA1的表达高于健康体检者,差异有统计学意义(P<0.001);在胃癌患者外周血中HMGA1的表达在患者的年龄、分化程度、Lauren分型、浸润程度及有无淋巴结转移等方面,差异具有统计学意义(P<0.001);胃癌患者行根治手术后第1天、第3天、第5天、第7天的HMGA1的表达高于胃癌患者手术前,差异有统计学意义(P<0.001);高表达HMGA1与胃癌的生存期有关,且肿瘤组织中HMGA1蛋白及mRNA的表达水平增加(P<0.05)。结论检测胃癌患者外周血和肿瘤组织中HMGA1的表达对临床的诊断和治疗有一定的参考价值。展开更多
背景与目的高迁移率族蛋白A2(High mobility group A2,HMGA2)是与染色体结合的非组蛋白,在许多恶性肿瘤中高表达并与预后有关。本研究探讨HMGA2在非小细胞肺癌(NSCLC)中的表达的临床特征及其意义。方法采用免疫组化SP法检测59例手术切...背景与目的高迁移率族蛋白A2(High mobility group A2,HMGA2)是与染色体结合的非组蛋白,在许多恶性肿瘤中高表达并与预后有关。本研究探讨HMGA2在非小细胞肺癌(NSCLC)中的表达的临床特征及其意义。方法采用免疫组化SP法检测59例手术切除非小细胞肺癌组织,10例非肿瘤组织中HMGA2表达。采用c2检验,Kaplan-Meier生存曲线和Cox回归分析,比较与HMGA2表达相关因素及其对生存期的影响。结果非小细胞肺癌组织中HMGA2表达率78%(46/59),其中肺鳞癌表达率70.4%(19/27),肺腺癌表达率82.1%(23/28),4例腺鳞癌均表达,而10例非肿瘤组织中均不表达。HMGA2表达与TNM分期,淋巴结转移相关(P<0.05)。Cox多因素回归分析显示TNM分期是本组患者独立预后因子。结论HMGA2在非小细胞肺癌中呈过度表达,与TNM分期、淋巴结转移相关。展开更多
试验对 HMGA1基因的两个 SNPs 位点进行分析,研究了 HMGA1基因核苷酸多态性与生长、饲料利用性状的关联性。利用大白猪和民猪重测序结果比较发现了 HMGA1基因的两个 SNPs 位点（g.-543 T 〉 C 和g.1356 C〉T）,利用 Sequenom 质谱测序...试验对 HMGA1基因的两个 SNPs 位点进行分析,研究了 HMGA1基因核苷酸多态性与生长、饲料利用性状的关联性。利用大白猪和民猪重测序结果比较发现了 HMGA1基因的两个 SNPs 位点（g.-543 T 〉 C 和g.1356 C〉T）,利用 Sequenom 质谱测序平台对杜洛克猪 g.-543 T〉C 和 g.1356 C〉T 位点进行基因分型,并分析该位点多态性与生长、饲料利用性状的关联性。结果发现,杜洛克猪群体 g.-543 T〉C 位点,CT 与 CC 基因型相比,90日龄体重升高了2.15 kg （P 〈0.05）,30 kg 体重日龄降低了3.21 d （P 〈0.05）,断奶重升高,剩余采食量降低,100 kg 体重日龄减少,30-100 kg 平均日增重、40-90 kg 平均日采食量、40-90 kg 平均日增重均降低。杜洛克猪群体 g.1356 C〉T 位点,CC 与 TT 基因型相比,90日龄体重升高了0.37 kg （P 〈0.05）；平均日采食量降低0.13 kg/d （P 〈0.05）,100 kg 背膘厚降低0.43 mm （P 〈0.05）,剩余采食量降低了70.42 g （P 〈0.05）,30 kg 体重日龄降低了0.56 d （P 〈0.05）；40-90 kg 平均日采食量降低了0.17 kg （P 〈0.05）,断奶重升高,100 kg 体重日龄增大,30-100、40-90 kg 平均日增重均降低；CT 与 TT 基因型结果与上结果类似。结果初步表明 HMGA1基因两个 SNPs 位点的突变有利于杜洛克猪的生长、饲料利用效率的提高。展开更多
目的:研究肝细胞癌(hepatocelluar carcinoma,HCC)中高迁移率族蛋白A1(high mobility group protein A1,HMGA1)的表达情况,探讨HMGA1基因在HCC中的表达与肝内转移以及临床病理参数之间的关系。方法:采用实时定量荧光聚合酶链反应(real-t...目的:研究肝细胞癌(hepatocelluar carcinoma,HCC)中高迁移率族蛋白A1(high mobility group protein A1,HMGA1)的表达情况,探讨HMGA1基因在HCC中的表达与肝内转移以及临床病理参数之间的关系。方法:采用实时定量荧光聚合酶链反应(real-time fluorescent quantitative polymerase chain reaction,QRT-PCR)检测52例肝细胞癌组织和配对的癌旁组织、10例正常肝脏组织中HMGA1mRNA的表达,用免疫组化法(immunohistochemistry,IHC)检测HMGA1蛋白在肝细胞癌中的表达,评价HMGA1的表达与HCC临床病理学因素的相关性。结果:肝癌组织中HMGA1mRNA的表达较正常肝脏中升高6.82倍,其中有、无肝内转移组较正常肝组织分别升高13.36倍和4.63倍(P<0.05)。HMGA1mRNA的表达与TNM分期、Edmonson分级、肝内转移有关(P<0.05)。肝细胞癌中有肝内转移组较无肝内转移组HMGA1蛋白表达明显增强。结论:HMGA1基因在肝细胞癌中的表达较正常肝脏组织中明显升高,并与肿瘤的肝内转移相关,有望成为肝细胞癌诊断和治疗的新靶点。展开更多
基金Fujian Provincial Health and Middleaged and Young Backbone Talents Training Project“The role and Mechanism of C53 in mcRPC Treatment of Drug Resistance”(2019-ZQN-77).
文摘Objective:Circular ribose nudeic acids(circRNAs)are implicated in tumor progression and drug resistance of prostate cancer(PCa).The current work explored the function of circ_0005203(aircTHSD4)in the malignancy and docetaxel(DTX)resistance of PCa.Methods:circTHSD4 expression within PCa as well as matched non-carcinoma samples was measured through real time reverse transcription quantitative polymerase chain reaction(RT-qPCR).In addition,a subcellular fraction assay was conducted to determine circTHSD4 subcellular localization within PCa cells.In addition,we performed a Western blot(WB)assay to detect high mobility.group A2 protein(HMGA2)levels.Besides,functional associations of two molecules were investigated through dual luciferase reporter assay.Cell Counting Kit(CCK)-8,colony formation together with Transwell assay was conducted to assess malignant phenotypes of PCa cells,whereas flow cytometry was performed to determine cell apoptosis.Furthermore,a xenograft mouse model was constructed to verify the effect of circTHSD4 on the carcinogenesis of PCa cells.Results:According to RT-qPCR results,circTHSD4 was up-regulated within PCa tissues and cells,which predicted the dismal prognostic outcome of PCa cases.circTHSD4 silencing within PCa cells markedly suppressed cell growth,migration,and colony fomation.circTHSD4 silencing remarkably elevated PCa cell apoptosis and carcinogenesis within the xenograft model.Further,circTHSD4 silencing enhanced docetaxel(DTX)sensitivity in PCa cells.Furthermore,we demonstrated that circTHSD4 modulated the malignancy of PCa cells by regulating HMGA2 expression through sponging miR 203.Conclusion:Together,our findings suggest that cirCTHSD4 overexpression could promote the malignant phenotype and DTX resistance in PCa through the regulation of the miR 203/HMGA2 axis.
文摘In the article“Long non-coding RNA LINC02163 accelerates malignant tumor behaviors in breast cancer by regulating the microRNA-511-3p/HMGA2 axis as a competing endogenous RNA”(Oncology Research,2020,Vol.28,No.5,pp.483–495.doi:10.3727/096504020X15928179818438),there was an error in the processing of data.To further confirm our observation,we repeated multiple experiments involving in this study,including Flow Cytometry,Transwell Cell Migration and Invasion Assays,Xenograft Tumor Model,and Western Blotting.We have revised the figures to correct these errors.Corrected versions of the Figs.2,4,5,6,and 7 are provided.The corrections do not change any results or conclusion of the article.We apologize for any inconvenience caused.
基金Supported by Natural Science Foundation of Anhui Province,No.2108085QH337Research Fund of Anhui Medical University,No.2022xkj156+1 种基金Key Projects of Anhui Provincial Department of Education,No.2023AH053330Anhui Institute of Translational Medicine Research Fund,No.2022zhyx-C88.
文摘BACKGROUND Gastric cancer(GC)is a common malignant tumor,long non-coding RNA and microRNA(miRNA)are important regulators that affect tumor proliferation,metastasis and chemotherapy resistance,and thus participate in tumor progression.CASC19 is a new bio-marker which can promote tumor invasion and metastasis.However,the mechanism by which CASC19 affects the progression of GC through miRNA is not clear.AIM To explore the role of the CASC19/miR-491-5p/HMGA2 regulatory axis in GC.METHODS To explore the expression and prognosis of CASC19 in GC through clinical samples,and investigate the effects of inhibiting CASC19 on the proliferation,migration,invasion and other functions of GC cells through cell counting Kit-8(CCK-8),ethynyldeoxyuridine,Wound healing assay,Transwell,Western blot and flow cytometry experiments.The effect of miR-491-5p and HMGA2 in GC were also proved.The regulatory relationship between CASC19 and miR-491-5p,miR-491-5p and HMGA2 were validated through Dual-luciferase reporter gene assay and reverse transcription PCR.Then CCK-8,Transwell,Wound healing assay,flow cytometry and animal experiments verify the role of CASC19/miR-491-5p/HMGA2 regulatory axis.RESULTS The expression level of CASC19 is related to the T stage,N stage,and tumor size of patients.Knockdown of the expression of CASC19 can inhibit the ability of proliferation,migration,invasion and EMT conversion of GC cells,and knocking down the expression of CASC19 can promote the apoptosis of GC cells.Increasing the expression of miR-491-5p can inhibit the proliferation of GC cells,miR-491-5p mimics can inhibit EMT conversion,and promote the apoptosis of GC cells,while decreasing the expression of miR-491-5p can promote the proliferation and EMT conversion and inhibit the apoptosis of GC cells.The expression of HMGA2 in GC tissues is higher than that in adjacent tissues.At the same time,the expression level of HMGA2 is related to the N and T stages of the patients.Reducing the level of HMGA2 can promote cell apoptosis and inhibit the proliferation of GC cells.Cell experiments and animal experiments have proved that CASC19 can regulates the expression of HMGA2 through miR-491-5p,thereby affecting the biological functions of GC.CONCLUSION CASC19 regulates the expression of HMGA2 through miR-491-5p to affect the development of GC.This axis may serve as a potential biomarker and therapeutic target of GC.
文摘目的探讨胃癌患者外周血及组织中高迁移率族蛋白A1(high mobility group AT-hook1,HMGA1)表达及临床意义。方法应用酶联免疫吸附实验及免疫组织化学染色胃癌患者病理石腊包埋组织进行外周血及组织中HMGA1的表达检测;检索数据库分析HMGA1在胃癌肿瘤组织的表达以及总生存期;采用Western Blot及qRT-PCR检测胃癌肿瘤组织及癌旁组织中HMGA1蛋白及mRNA的表达。结果胃癌患者外周血中HMGA1的表达高于健康体检者,差异有统计学意义(P<0.001);在胃癌患者外周血中HMGA1的表达在患者的年龄、分化程度、Lauren分型、浸润程度及有无淋巴结转移等方面,差异具有统计学意义(P<0.001);胃癌患者行根治手术后第1天、第3天、第5天、第7天的HMGA1的表达高于胃癌患者手术前,差异有统计学意义(P<0.001);高表达HMGA1与胃癌的生存期有关,且肿瘤组织中HMGA1蛋白及mRNA的表达水平增加(P<0.05)。结论检测胃癌患者外周血和肿瘤组织中HMGA1的表达对临床的诊断和治疗有一定的参考价值。
文摘背景与目的高迁移率族蛋白A2(High mobility group A2,HMGA2)是与染色体结合的非组蛋白,在许多恶性肿瘤中高表达并与预后有关。本研究探讨HMGA2在非小细胞肺癌(NSCLC)中的表达的临床特征及其意义。方法采用免疫组化SP法检测59例手术切除非小细胞肺癌组织,10例非肿瘤组织中HMGA2表达。采用c2检验,Kaplan-Meier生存曲线和Cox回归分析,比较与HMGA2表达相关因素及其对生存期的影响。结果非小细胞肺癌组织中HMGA2表达率78%(46/59),其中肺鳞癌表达率70.4%(19/27),肺腺癌表达率82.1%(23/28),4例腺鳞癌均表达,而10例非肿瘤组织中均不表达。HMGA2表达与TNM分期,淋巴结转移相关(P<0.05)。Cox多因素回归分析显示TNM分期是本组患者独立预后因子。结论HMGA2在非小细胞肺癌中呈过度表达,与TNM分期、淋巴结转移相关。
文摘试验对 HMGA1基因的两个 SNPs 位点进行分析,研究了 HMGA1基因核苷酸多态性与生长、饲料利用性状的关联性。利用大白猪和民猪重测序结果比较发现了 HMGA1基因的两个 SNPs 位点（g.-543 T 〉 C 和g.1356 C〉T）,利用 Sequenom 质谱测序平台对杜洛克猪 g.-543 T〉C 和 g.1356 C〉T 位点进行基因分型,并分析该位点多态性与生长、饲料利用性状的关联性。结果发现,杜洛克猪群体 g.-543 T〉C 位点,CT 与 CC 基因型相比,90日龄体重升高了2.15 kg （P 〈0.05）,30 kg 体重日龄降低了3.21 d （P 〈0.05）,断奶重升高,剩余采食量降低,100 kg 体重日龄减少,30-100 kg 平均日增重、40-90 kg 平均日采食量、40-90 kg 平均日增重均降低。杜洛克猪群体 g.1356 C〉T 位点,CC 与 TT 基因型相比,90日龄体重升高了0.37 kg （P 〈0.05）；平均日采食量降低0.13 kg/d （P 〈0.05）,100 kg 背膘厚降低0.43 mm （P 〈0.05）,剩余采食量降低了70.42 g （P 〈0.05）,30 kg 体重日龄降低了0.56 d （P 〈0.05）；40-90 kg 平均日采食量降低了0.17 kg （P 〈0.05）,断奶重升高,100 kg 体重日龄增大,30-100、40-90 kg 平均日增重均降低；CT 与 TT 基因型结果与上结果类似。结果初步表明 HMGA1基因两个 SNPs 位点的突变有利于杜洛克猪的生长、饲料利用效率的提高。
