目的:探讨高迁移率族核小体结合域5(high mobility group nucleosome 5,HMGN5)蛋白在上皮性卵巢恶性肿瘤中的表达及其临床意义。方法:本研究共纳入手术切除组织石蜡标本50例,采用免疫组化方法检测上皮性卵巢恶性肿瘤标本35例、上皮性卵...目的:探讨高迁移率族核小体结合域5(high mobility group nucleosome 5,HMGN5)蛋白在上皮性卵巢恶性肿瘤中的表达及其临床意义。方法:本研究共纳入手术切除组织石蜡标本50例,采用免疫组化方法检测上皮性卵巢恶性肿瘤标本35例、上皮性卵巢良性肿瘤标本15例中HMGN5蛋白的表达情况,并分析HMGN5蛋白阳性表达率与上皮性卵巢恶性肿瘤临床病理特征之间的关系。结果:HMGN5蛋白在上皮性卵巢恶性肿瘤组织中的阳性表达率明显高于上皮性卵巢良性肿瘤组织,差异有统计学意义(P<0.05);HMGN5蛋白在上皮性卵巢恶性肿瘤组织中的阳性表达率与年龄、有无腹水、是否为浆液性上皮性癌无关(P>0.05),而与组织分化程度、手术病理分期有关(P<0.05)。HMGN5蛋白在中低分化上皮性卵巢恶性肿瘤组织中的阳性表达率明显高于高分化上皮性卵巢恶性肿瘤组织,差异有统计学意义(P<0.05);HMGN5蛋白在手术病理分期为Ⅲ-Ⅳ期上皮性卵巢恶性肿瘤组织中的阳性表达率明显高于Ⅰ-Ⅱ期上皮性卵巢恶性肿瘤组织,差异有统计学意义(P<0.05)。结论:HMGN5蛋白可能在上皮性卵巢恶性肿瘤的发生以及恶性进展中发挥作用,对上皮性卵巢恶性肿瘤的诊断及病情评估有一定价值,有可能是上皮性卵巢恶性肿瘤治疗的一个潜在靶点。展开更多
We investigated the importance of HMGN5, a nuclear protein that binds to nucleosomes, unfolds chromatin, and affects transcription, in the LNCaP prostate cancer cell line. We also examined the molecular mechanisms tha...We investigated the importance of HMGN5, a nuclear protein that binds to nucleosomes, unfolds chromatin, and affects transcription, in the LNCaP prostate cancer cell line. We also examined the molecular mechanisms that promote apoptosis of LNCaP cells after infection with small interfering RNA （siRNA） targeting HMGN5 （siRNA-HMGN5）. The androgen-dependent LNCaP human prostate cancer cells were infected with siRNA-HMGN5. Apoptosis was detected using the Annexin V-PE/7-AAD double staining and the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling （TUNEL） assays. Mitochondrial membrane potential was measured by JC-1 staining. HMGN5and GAPDHmRNA expression were determined using real-time PCR. Bcl-2 and other apoptosis-related protein levels were determined by Western blot analysis. Caspase activity was measured by cleavage of the caspase substrate. Infection with siRNA targeting HMGN5 efficiently and specifically reduced the HMGN5 expression in LNCaP cells. The downregulation of HMGN5 induced remarkable apoptosis of LNCaP cells and resulted in the reduction of mitochondrial membrane potential. The induction of cell apoptosis was accompanied by the upregulation of Bax, the Bax/Bcl-2 ratio and the activation of caspase3. The HMGN5-targeted siRNA was effective in downregulating the expression of HMGN5 in androgen-dependent prostate cancer cells and inducing cell apoptosis via the regulation of a caspase-related mitochondrial pathway and Bcl-2 family proteins. This study suggests that HMGN5 may be a potential molecular target with therapeutic relevance for the treatment of prostate cancer.展开更多
目的:研究非小细胞肺癌中诱导细胞凋亡相关基因 19(gene associated with retinoid/interferon induced mortality 19,GRIM-19)联合高迁移率蛋白5(high mobility group protein 5,HMGN 5)的表达及其与临床各病理学特征间的关系。方法:...目的:研究非小细胞肺癌中诱导细胞凋亡相关基因 19(gene associated with retinoid/interferon induced mortality 19,GRIM-19)联合高迁移率蛋白5(high mobility group protein 5,HMGN 5)的表达及其与临床各病理学特征间的关系。方法:应用免疫组织化学技术检测67例非小细胞肺癌组织和39例远端正常组织(距癌灶>5 cm)中GRIM-19、HMGN 5的表达情况,并分析其与各临床病理特征之间的可能关系。结果:67例非小细胞肺癌组织中,GRIM-19阳性表达率为53.7%,低于在远端正常组织中的表达( P <0.01);HMGN 5阳性表达率为59.7%,高于在远端正常组织中的表达( P <0.01)。非小细胞肺癌组织中GRIM-19 、HMGN 5的表达可能有相关性( P <0.01)。结论:GRIM-19、HMGN 5在非小细胞肺癌中有不同程度的表达,可能与非小细胞肺癌发生、发展有关;临床检测非小细胞肺癌中GRIM-19、HMGN 5的表达,可能为治疗非小细胞肺癌提供新的理论研究基础和思路。展开更多
高迁移率族核小体结合蛋白5(high-mobility group nucleosome-binding protein 5,HMGN5)属于高迁移率族N(HMGN)家族,是一种广泛存在于真核生物染色质内的非组蛋白,可通过与核小体结合调节染色质结构的“开放”与“关闭”,从而影响下游...高迁移率族核小体结合蛋白5(high-mobility group nucleosome-binding protein 5,HMGN5)属于高迁移率族N(HMGN)家族,是一种广泛存在于真核生物染色质内的非组蛋白,可通过与核小体结合调节染色质结构的“开放”与“关闭”,从而影响下游基因的表达。多项临床研究发现HMGN5在不同类型肿瘤组织中都存在表达上调,可调控包括人前列腺癌、膀胱癌、肺癌、乳腺癌、胶质瘤在内的多种肿瘤细胞的增殖、迁移及耐药等生物学过程。本文总结了HMGN5在不同肿瘤中的表达情况与意义,以及它在肿瘤发生发展中涉及的分子调控,并探讨了其在肿瘤治疗中的潜在价值。展开更多
文摘目的:探讨高迁移率族核小体结合域5(high mobility group nucleosome 5,HMGN5)蛋白在上皮性卵巢恶性肿瘤中的表达及其临床意义。方法:本研究共纳入手术切除组织石蜡标本50例,采用免疫组化方法检测上皮性卵巢恶性肿瘤标本35例、上皮性卵巢良性肿瘤标本15例中HMGN5蛋白的表达情况,并分析HMGN5蛋白阳性表达率与上皮性卵巢恶性肿瘤临床病理特征之间的关系。结果:HMGN5蛋白在上皮性卵巢恶性肿瘤组织中的阳性表达率明显高于上皮性卵巢良性肿瘤组织,差异有统计学意义(P<0.05);HMGN5蛋白在上皮性卵巢恶性肿瘤组织中的阳性表达率与年龄、有无腹水、是否为浆液性上皮性癌无关(P>0.05),而与组织分化程度、手术病理分期有关(P<0.05)。HMGN5蛋白在中低分化上皮性卵巢恶性肿瘤组织中的阳性表达率明显高于高分化上皮性卵巢恶性肿瘤组织,差异有统计学意义(P<0.05);HMGN5蛋白在手术病理分期为Ⅲ-Ⅳ期上皮性卵巢恶性肿瘤组织中的阳性表达率明显高于Ⅰ-Ⅱ期上皮性卵巢恶性肿瘤组织,差异有统计学意义(P<0.05)。结论:HMGN5蛋白可能在上皮性卵巢恶性肿瘤的发生以及恶性进展中发挥作用,对上皮性卵巢恶性肿瘤的诊断及病情评估有一定价值,有可能是上皮性卵巢恶性肿瘤治疗的一个潜在靶点。
文摘We investigated the importance of HMGN5, a nuclear protein that binds to nucleosomes, unfolds chromatin, and affects transcription, in the LNCaP prostate cancer cell line. We also examined the molecular mechanisms that promote apoptosis of LNCaP cells after infection with small interfering RNA （siRNA） targeting HMGN5 （siRNA-HMGN5）. The androgen-dependent LNCaP human prostate cancer cells were infected with siRNA-HMGN5. Apoptosis was detected using the Annexin V-PE/7-AAD double staining and the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling （TUNEL） assays. Mitochondrial membrane potential was measured by JC-1 staining. HMGN5and GAPDHmRNA expression were determined using real-time PCR. Bcl-2 and other apoptosis-related protein levels were determined by Western blot analysis. Caspase activity was measured by cleavage of the caspase substrate. Infection with siRNA targeting HMGN5 efficiently and specifically reduced the HMGN5 expression in LNCaP cells. The downregulation of HMGN5 induced remarkable apoptosis of LNCaP cells and resulted in the reduction of mitochondrial membrane potential. The induction of cell apoptosis was accompanied by the upregulation of Bax, the Bax/Bcl-2 ratio and the activation of caspase3. The HMGN5-targeted siRNA was effective in downregulating the expression of HMGN5 in androgen-dependent prostate cancer cells and inducing cell apoptosis via the regulation of a caspase-related mitochondrial pathway and Bcl-2 family proteins. This study suggests that HMGN5 may be a potential molecular target with therapeutic relevance for the treatment of prostate cancer.
文摘目的:研究非小细胞肺癌中诱导细胞凋亡相关基因 19(gene associated with retinoid/interferon induced mortality 19,GRIM-19)联合高迁移率蛋白5(high mobility group protein 5,HMGN 5)的表达及其与临床各病理学特征间的关系。方法:应用免疫组织化学技术检测67例非小细胞肺癌组织和39例远端正常组织(距癌灶>5 cm)中GRIM-19、HMGN 5的表达情况,并分析其与各临床病理特征之间的可能关系。结果:67例非小细胞肺癌组织中,GRIM-19阳性表达率为53.7%,低于在远端正常组织中的表达( P <0.01);HMGN 5阳性表达率为59.7%,高于在远端正常组织中的表达( P <0.01)。非小细胞肺癌组织中GRIM-19 、HMGN 5的表达可能有相关性( P <0.01)。结论:GRIM-19、HMGN 5在非小细胞肺癌中有不同程度的表达,可能与非小细胞肺癌发生、发展有关;临床检测非小细胞肺癌中GRIM-19、HMGN 5的表达,可能为治疗非小细胞肺癌提供新的理论研究基础和思路。
文摘高迁移率族核小体结合蛋白5(high-mobility group nucleosome-binding protein 5,HMGN5)属于高迁移率族N(HMGN)家族,是一种广泛存在于真核生物染色质内的非组蛋白,可通过与核小体结合调节染色质结构的“开放”与“关闭”,从而影响下游基因的表达。多项临床研究发现HMGN5在不同类型肿瘤组织中都存在表达上调,可调控包括人前列腺癌、膀胱癌、肺癌、乳腺癌、胶质瘤在内的多种肿瘤细胞的增殖、迁移及耐药等生物学过程。本文总结了HMGN5在不同肿瘤中的表达情况与意义,以及它在肿瘤发生发展中涉及的分子调控,并探讨了其在肿瘤治疗中的潜在价值。
