Catharanthus roseus is the sole source of two most important monoterpene indole alkaloid （MIA） anti- cancer agents： vinblastine and vincristine. MIAs possess a terpene and an indole moiety derived from terpenoid an...Catharanthus roseus is the sole source of two most important monoterpene indole alkaloid （MIA） anti- cancer agents： vinblastine and vincristine. MIAs possess a terpene and an indole moiety derived from terpenoid and shikimate pathways, respectively. Geranyl diphosphate （GPP）, the entry point to the formation of terpene moiety, is a product of the condensation of isopentenyl diphosphate （IPP） and dimethylallyl diphosphate （DMAPP） by GPP synthase （GPPS）. Here, we report three genes encoding proteins with sequence similarity to large subunit （CrGPPS.LSU） and small subunit （CrGPPS.SSU） of heteromeric GPPSs, and a homomeric GPPSs. CrGPPS.LSU is a bifunctional enzyme producing both GPP and geranyl geranyl diphosphate （GGPP）, CrGPPS.SSU is inactive, whereas CrGPPS is a homomeric enzyme forming GPP. Co-expression of both subunits in Escherichia coil resulted in heteromeric enzyme with enhanced activity producing only GPR While CrGPPS.LSU and CrGPPS showed higher expression in older and younger leaves, respectively, CrGPPS.SSU showed an increasing trend and decreased gradually. Methyl jasmonate （MelA） treatment of leaves sig- nificantly induced the expression of only CrGPPS.SSU. GFP localization indicated that CrGPPS.SSU is plastidial whereas CrGPPS is mitochondrial. Transient overexpression of AmGPPS.SSU in C. roseus leaves resulted in increased vindoline, immediate monomeric precursor of vinblastine and vincristine. Although C. roseus has both heteromeric and homomeric GPPS enzymes, our results implicate the involvement of only heteromeric GPPS with CrGPPS.SSU regulating the GPP flux for MIA biosynthesis.展开更多
In order to fast analyze the aircraft Radar Cross Section(RCS) and accurately reduce it with Radar Absorbing Materials(RAM), a comprehensive analysis method based on Higher-Order Method of Moments(HOMOM), termed Local...In order to fast analyze the aircraft Radar Cross Section(RCS) and accurately reduce it with Radar Absorbing Materials(RAM), a comprehensive analysis method based on Higher-Order Method of Moments(HOMOM), termed Locally Coating Method(LCM), is proposed in this paper. There are two steps to fast analyze coatings for RCS reduction in this method: analyze the RCS of various parts before coating the aircraft;model a coating over the aircraft and analyze the wave absorbing effect of it. The aircraft RCS is calculated as a whole but analyzed in various parts by LCM, and thus the RCS contribution of different parts can be compared without disturbing the current continuity. A model expansion algorithm is also presented in LCM to model absorption coatings on specified aircraft parts for later stage RCS calculation of the coated aircraft.展开更多
文摘Catharanthus roseus is the sole source of two most important monoterpene indole alkaloid （MIA） anti- cancer agents： vinblastine and vincristine. MIAs possess a terpene and an indole moiety derived from terpenoid and shikimate pathways, respectively. Geranyl diphosphate （GPP）, the entry point to the formation of terpene moiety, is a product of the condensation of isopentenyl diphosphate （IPP） and dimethylallyl diphosphate （DMAPP） by GPP synthase （GPPS）. Here, we report three genes encoding proteins with sequence similarity to large subunit （CrGPPS.LSU） and small subunit （CrGPPS.SSU） of heteromeric GPPSs, and a homomeric GPPSs. CrGPPS.LSU is a bifunctional enzyme producing both GPP and geranyl geranyl diphosphate （GGPP）, CrGPPS.SSU is inactive, whereas CrGPPS is a homomeric enzyme forming GPP. Co-expression of both subunits in Escherichia coil resulted in heteromeric enzyme with enhanced activity producing only GPR While CrGPPS.LSU and CrGPPS showed higher expression in older and younger leaves, respectively, CrGPPS.SSU showed an increasing trend and decreased gradually. Methyl jasmonate （MelA） treatment of leaves sig- nificantly induced the expression of only CrGPPS.SSU. GFP localization indicated that CrGPPS.SSU is plastidial whereas CrGPPS is mitochondrial. Transient overexpression of AmGPPS.SSU in C. roseus leaves resulted in increased vindoline, immediate monomeric precursor of vinblastine and vincristine. Although C. roseus has both heteromeric and homomeric GPPS enzymes, our results implicate the involvement of only heteromeric GPPS with CrGPPS.SSU regulating the GPP flux for MIA biosynthesis.
基金supported by the National Key Research and Development Program of China (No. 2017YFB0202102),the National Key Research and Development Program of China (No. 2016YFE0121600)the China Postdoctoral Science Foundation funded project (No. 2017M613068)+2 种基金the National High Technology Research and Development Program of China (863 Program) (No. 2014AA01A302)the Key Research and Development Program of Shandong Province, China (No. 2015GGX101028)the Special Program for Applied Research on Super Computation of the NSFC (National Natural Science Foundation of China)-Guangdong Joint Fund, China (the second phase) (No. U1501501)
文摘In order to fast analyze the aircraft Radar Cross Section(RCS) and accurately reduce it with Radar Absorbing Materials(RAM), a comprehensive analysis method based on Higher-Order Method of Moments(HOMOM), termed Locally Coating Method(LCM), is proposed in this paper. There are two steps to fast analyze coatings for RCS reduction in this method: analyze the RCS of various parts before coating the aircraft;model a coating over the aircraft and analyze the wave absorbing effect of it. The aircraft RCS is calculated as a whole but analyzed in various parts by LCM, and thus the RCS contribution of different parts can be compared without disturbing the current continuity. A model expansion algorithm is also presented in LCM to model absorption coatings on specified aircraft parts for later stage RCS calculation of the coated aircraft.
