LncRNAHOTAIRM1/HOXA1促进胶质瘤增殖迁移和侵袭

目的探究长链非编码RNA HOTAIRM1(LncRNA HOTAIRM1)靶向调控HOXA1对胶质瘤细胞增殖、迁移和侵袭的影响。方法收集2020年10月至2021年12月于温州市中心医院接受手术治疗的60例胶质瘤患者的肿瘤组织及癌旁正常脑组织标本,采用实时荧光定量聚合酶链式反应(qRT-PCR)测定并比较正常脑组织和胶质瘤组织中HOTAIRM1 mRNA和HOXA1 mRNA表达;构建干扰LncRNA HOTAIRM1的慢病毒载体(sh-HOTAIRM1)及其阴性对照载体(sh-NC)、HOXA1过表达载体(pcDNA3.1-HOXA1)及其阴性对照载体(pcDNA3.1-NC)。培养人胶质瘤细胞LN229并进行转染干预,分为对照组(无转染)、sh-HOTAIRM1组(转染sh-HOTAIRM1)、sh-NC组(转染sh-NC)、sh-HOTAIRM1+HOXA1组(转染sh-HOTAIRM1和pcDNA3.1-HOXA1)、sh-HOTAIRM1+NC组(转染sh-HOTAIRM1和pcDNA3.1-NC)。采用MTT法检测转染后各组细胞增殖能力,采用流式细胞仪检测各组细胞凋亡水平,采用Transwell实验测定各组细胞侵袭和迁移能力。结果胶质瘤组织中HOTAIRM1 mRNA和HOXA1 mRNA的表达水平高于正常脑组织(均P<0.05);LN229细胞转染48h后,sh-HOTAIRM1组和sh-HOTAIRM1+NC组细胞中HOTAIRM1 mRNA和HOXA1 mRNA表达水平均低于对照组和sh-NC组(均P<0.05),细胞凋亡率高于对照组和sh-NC组(均P<0.05),迁移细胞数与侵袭细胞数均少于对照组和sh-NC组(均P<0.05);sh-HOTAIRM1+HOXA1组细胞中HOXA1 mRNA表达水平高于sh-HOTAIRM1组和sh-HOTAIRM1+NC(均P<0.05),凋亡率低于sh-HOTAIRM1组和sh-HOTAIRM1+NC组(均P<0.05),细胞迁移细胞数与侵袭细胞数均多于sh-HOTAIRM1组和sh-HOTAIRM1+NC组(均P<0.05)。转染后24~72h,各组细胞增殖活性均逐渐升高(均P<0.05);在不同时间点,sh-HOTAIRM1组和sh-HOTAIRM1+NC组细胞增殖活性均弱于对照组和sh-NC组(均P<0.05),sh-HOTAIRM1+HOXA1组细胞增殖活性强于sh-HOTAIRM1组和sh-HOTAIRM1+NC组(均P<0.05)。结论LncRNAHOTAIRM1和HOXA1在胶质瘤组织中呈高表达状态,HOTAIRM1或可通过靶向调控HOXA1促进胶质瘤细胞增殖,抑制其凋亡,并增强胶质瘤细胞迁移和侵袭能力。