目的研究同源盒基因A7(homeobox protein hox-A7,HOXA7)mRNA在口腔鳞状细胞癌组织(oral squamous cell carcinoma,OSCC)中的表达。方法收集20例口腔正常粘膜组织及42例OSCC组织,利用实时荧光定量聚合酶链反应(real-time quantitative pr...目的研究同源盒基因A7(homeobox protein hox-A7,HOXA7)mRNA在口腔鳞状细胞癌组织(oral squamous cell carcinoma,OSCC)中的表达。方法收集20例口腔正常粘膜组织及42例OSCC组织,利用实时荧光定量聚合酶链反应(real-time quantitative program control register,Real-Time q PCR)检测HOXA7mRNA的相对表达情况。结果对比口腔黏膜组织,HOXA7mRNA在OSCC中高表达(z=-5.739,P<0.050),不同分化程度(z=-0.863,P<0.05),不同临床分期(z=-0.756,P<0.05),组间差异有统计学意义。不同年龄(z=-0.619,P>0.05)、不同性别(z=-1.801,P>0.05),组间差异无统计学意义。结论 HOXA7mRNA在口腔鳞状细胞癌组织中高表达,并且与肿瘤分化和临床分期有关,HOXA7可能作为口腔癌治疗和口腔鳞癌早期诊断的潜在生物标记物。展开更多
Stem cell senescence is characterized by progressive functional dysfunction and secretory phenotypic changes including decreased proliferation,dysfunction of osteogenic and angiogenic differentiation,increased secreti...Stem cell senescence is characterized by progressive functional dysfunction and secretory phenotypic changes including decreased proliferation,dysfunction of osteogenic and angiogenic differentiation,increased secretion of the senescence-associated secretory phenotype(SASP),which bring difficulties for bone repair.Rescuing or delaying senescence of aged bone marrow mesenchymal stem cells(O-BMSCs)was considered as effective strategy for bone regeneration in aging microenvironment.Magnesium(Mg)ion released from bioceramics was reported to facilitate bone regeneration via enhancing osteogenesis and alleviating senescence.In this study,Akermanite biocreamics(Akt)containing Mg ion as a model was demonstrated to promote osteogenesis and angiogenesis effects of O-BMSCs by activating the MAPK signaling pathway in vitro.Moreover,the enhanced osteogenesis effects might be attributed to enhanced Mg-containing Akt-mediated exosomal miR-196a-5p cargo targeting Hoxa7 and activation of MAPK signaling pathway.Furthermore,the in vivo study confirmed that 3D-printed porous Mg-containing Akt scaffolds effectively increased bone regeneration in cranial defects of aged rats.The current results indicated that the exosomal-miR-196a-5p/Hoxa7/MAPK signaling axis might be the potential mechanism underlying Akt-mediated osteogenesis.The exosome-meditaed therapy stimulated by the released Mg ion contained in Akt biocreamics or other biomaterials might serve as a candidate strategy for bone repair in aged individuals.展开更多
文摘目的研究同源盒基因A7(homeobox protein hox-A7,HOXA7)mRNA在口腔鳞状细胞癌组织(oral squamous cell carcinoma,OSCC)中的表达。方法收集20例口腔正常粘膜组织及42例OSCC组织,利用实时荧光定量聚合酶链反应(real-time quantitative program control register,Real-Time q PCR)检测HOXA7mRNA的相对表达情况。结果对比口腔黏膜组织,HOXA7mRNA在OSCC中高表达(z=-5.739,P<0.050),不同分化程度(z=-0.863,P<0.05),不同临床分期(z=-0.756,P<0.05),组间差异有统计学意义。不同年龄(z=-0.619,P>0.05)、不同性别(z=-1.801,P>0.05),组间差异无统计学意义。结论 HOXA7mRNA在口腔鳞状细胞癌组织中高表达,并且与肿瘤分化和临床分期有关,HOXA7可能作为口腔癌治疗和口腔鳞癌早期诊断的潜在生物标记物。
基金support National Natural Science Foundation of China(No.81970973)Science and Technology Commission of Shanghai Municipality(No.20ZR1432200,21140900102,21490711700,22010502600)+3 种基金Disciplinary Characteristic Biobank Project of Ninth People’s Hospital affiliated to Shanghai Jiao Tong University School of Medicine(No.YBKB202110)Cross Disciplinary Research Fund of Shanghai Ninth People’s Hospital,Shanghai JiaoTong University School of Medicine(No.JYJC202219)Shanghai’s Top Priority Research Center(No.2022ZZ01017)CAMS Innovation Fund for Medical Sciences(No.CIFMS,2019-I2M-5-037).
文摘Stem cell senescence is characterized by progressive functional dysfunction and secretory phenotypic changes including decreased proliferation,dysfunction of osteogenic and angiogenic differentiation,increased secretion of the senescence-associated secretory phenotype(SASP),which bring difficulties for bone repair.Rescuing or delaying senescence of aged bone marrow mesenchymal stem cells(O-BMSCs)was considered as effective strategy for bone regeneration in aging microenvironment.Magnesium(Mg)ion released from bioceramics was reported to facilitate bone regeneration via enhancing osteogenesis and alleviating senescence.In this study,Akermanite biocreamics(Akt)containing Mg ion as a model was demonstrated to promote osteogenesis and angiogenesis effects of O-BMSCs by activating the MAPK signaling pathway in vitro.Moreover,the enhanced osteogenesis effects might be attributed to enhanced Mg-containing Akt-mediated exosomal miR-196a-5p cargo targeting Hoxa7 and activation of MAPK signaling pathway.Furthermore,the in vivo study confirmed that 3D-printed porous Mg-containing Akt scaffolds effectively increased bone regeneration in cranial defects of aged rats.The current results indicated that the exosomal-miR-196a-5p/Hoxa7/MAPK signaling axis might be the potential mechanism underlying Akt-mediated osteogenesis.The exosome-meditaed therapy stimulated by the released Mg ion contained in Akt biocreamics or other biomaterials might serve as a candidate strategy for bone repair in aged individuals.
