Objective：Homeobox B9 （HOXB9） is proposed to be involved in tumor angiogenesis and metastasis.We investigated the role of HOXB9 in the progression of colon cancer.Methods：HOXB9 expression was investigated by immun...Objective：Homeobox B9 （HOXB9） is proposed to be involved in tumor angiogenesis and metastasis.We investigated the role of HOXB9 in the progression of colon cancer.Methods：HOXB9 expression was investigated by immunohistochemically and Western blotting in 128 colon cancer patients and the results were analyzed statistically associated with clinicopathological data and survival of the patients.The effect of HOXB9 on cell invasion and metastases abilities were analyzed in vitro and in vivo.Results：HOXB9 is overexpressed in colon cancer tissues and significandy correlated with metastasis and poor survival of patients （P＜0.05,respectively）.Additionally,high levels of expression of HOXB9 were observed in metastatic lymph nodes.The down-regulation of HOXB9 expression can inhibit the migration and invasive ability of colon cancer cells,while exogenous expression of HOXB9 in colon cancer cells enhanced cell migration and invasiveness.Moreover,stable knockdown of HOXB9 reduced the liver and lung metastasis of colon cancer in vivo.Conclusions：HOXB9 may play an important role in the invasion and metastasis of colon cancer cells and may be a useful biomarker for metastasis and prognostic of colon cancer.展开更多
Pancreatic ductal adenocarcinoma(PDAC)is the ninth most common human malignancy and the sixth leading cause of cancer-related death in China.AcK27-HOXB9 is a newly identified HOXB9 post-transcriptional modification th...Pancreatic ductal adenocarcinoma(PDAC)is the ninth most common human malignancy and the sixth leading cause of cancer-related death in China.AcK27-HOXB9 is a newly identified HOXB9 post-transcriptional modification that can predict the outcome in lung adenocarcinoma and colon cancer well.However,the role of AcK27-HOXB9 in PDAC is unclear.The present study aims to investigate the differential diagnostic role of patients with AcK27-HOXB9 PDAC.Tissue microarrays consisting of 162 pancreatic tumor tissue samples from patients with PDAC and paired normal subjects were used to examine HOXB9 and AcK27-HOXB9 levels and localizations by immunohistochemical analysis and Western blot assay,respectively.HOXB9 was upregulated(P<0.0001),and AcK27-HOXB9(P=0.0023)was downregulated in patients with PDAC.HOXB9 promoted(P=0.0115),while AcK27-HOXB9(P=0.0279)inhibited PDAC progression.AcK27-HOXB9 predicted favorable outcome in patients with PDAC(P=0.0412).AcK27-HOXB9 also suppressed PDAC cell migration in a cell migration assay.The results of this study showed that HOXB9 promoted and AcK27-HOXB9 suppressed PDAC progression.The determination of ratio between HOXB9 and AcK27-HOXB9 exhibited potential diagnostic value in patients with PDAC.展开更多
目的 HOXB9(homeobox B9)是HOX转录因子家族的一员,参与多种肿瘤的发生发展。本研究旨在探讨HOXB9基因在甲状腺乳头状癌中的表达,及沉默HOXB9基因对TPC-1细胞增殖与侵袭转移能力的影响。方法收集2016-06-01—2017-06-01潍坊市中医院收治...目的 HOXB9(homeobox B9)是HOX转录因子家族的一员,参与多种肿瘤的发生发展。本研究旨在探讨HOXB9基因在甲状腺乳头状癌中的表达,及沉默HOXB9基因对TPC-1细胞增殖与侵袭转移能力的影响。方法收集2016-06-01—2017-06-01潍坊市中医院收治的88例甲状腺癌根治术患者组织标本,每例组织标本包括癌组织和癌旁组织(距离肿瘤组织1~2cm并经病理确诊的非癌组织)。利用实时定量聚合酶链反应(quantitative real time polymerase chain reaction,qRT-PCR)检测甲状腺乳头状癌组织和癌周组织中HOXB9基因表达。构建HOXB9的siRNA瞬时转染细胞TPC-1,应用蛋白质印迹法检测siRNA的沉默效果。通过MTT试验检测细胞生长能力、平板克隆试验检测细胞增殖能力、Transwell小室侵袭与迁移试验检测TPC-1细胞的侵袭与迁移能力,应用qRT-PCR及蛋白质印迹法检测转移相关蛋白MMP2与CXCR4在mRNA及蛋白水平表达的变化。结果甲状腺乳头状癌组织中HOXB9表达水平0.3±0.2,明显低于癌旁组织的1±0.2,t=4.287,P=0.013。沉默HOXB9基因后,分别在24、48及72h检测TPC-1细胞生长能力,结果显示,siHOXB9-TPC-1试验组(F=120.527,P<0.001)的生长能力明显高于sicontrol-TPC-1对照组(F=27.574,P<0.001)。siHOXB9-TPC-1试验组的克隆数90±10.8,高于sicontrol-TPC-1对照组的35±3.4,P<0.001。siHOXB9-TPC-1试验组迁移细胞数目107±5.2,高于sicontrol-TPC-1对照组的53±3.8,P<0.001;siHOXB9-TPC-1试验组侵袭细胞数目80±4.7,高于sicontrol-TPC-1对照组相的46±3.2,P<0.001。在siHOXB9-TPC-1试验组中,沉默HOXB9后MMP2和CXCR4表达明显上调。结论HOXB9基因是一个甲状腺乳头状癌的肿瘤抑制因子,沉默HOXB9基因可促进甲状腺乳头状癌的生长增殖及侵袭转移,为靶向治疗甲状腺癌提供新的思路。展开更多
基金supported by grants from the National Natural Science Foundation of China(No.81060196)the Scientific Research Project Foundation of Jiangxi Provincial Education Department(No.GJJ11333)Jiangxi Provincial Major Disciplines of Academic and Technical Leaders Project(No.20113BCB22004)
文摘Objective：Homeobox B9 （HOXB9） is proposed to be involved in tumor angiogenesis and metastasis.We investigated the role of HOXB9 in the progression of colon cancer.Methods：HOXB9 expression was investigated by immunohistochemically and Western blotting in 128 colon cancer patients and the results were analyzed statistically associated with clinicopathological data and survival of the patients.The effect of HOXB9 on cell invasion and metastases abilities were analyzed in vitro and in vivo.Results：HOXB9 is overexpressed in colon cancer tissues and significandy correlated with metastasis and poor survival of patients （P＜0.05,respectively）.Additionally,high levels of expression of HOXB9 were observed in metastatic lymph nodes.The down-regulation of HOXB9 expression can inhibit the migration and invasive ability of colon cancer cells,while exogenous expression of HOXB9 in colon cancer cells enhanced cell migration and invasiveness.Moreover,stable knockdown of HOXB9 reduced the liver and lung metastasis of colon cancer in vivo.Conclusions：HOXB9 may play an important role in the invasion and metastasis of colon cancer cells and may be a useful biomarker for metastasis and prognostic of colon cancer.
基金supported by grants from the Ministry of Science and Technology of China(Nos.2016YFC1302103 and 2015CB553906)the National Natural Science Foundation of China(Nos.81730071,81230051,81472734,and 31170711)+1 种基金the Beijing Natural Science Foundation(Nos.7120002 and 7171005)This work was also supported by a grant from the National Natural Science Foundation of China(No.81773199)to Jun Zhan.
文摘Pancreatic ductal adenocarcinoma(PDAC)is the ninth most common human malignancy and the sixth leading cause of cancer-related death in China.AcK27-HOXB9 is a newly identified HOXB9 post-transcriptional modification that can predict the outcome in lung adenocarcinoma and colon cancer well.However,the role of AcK27-HOXB9 in PDAC is unclear.The present study aims to investigate the differential diagnostic role of patients with AcK27-HOXB9 PDAC.Tissue microarrays consisting of 162 pancreatic tumor tissue samples from patients with PDAC and paired normal subjects were used to examine HOXB9 and AcK27-HOXB9 levels and localizations by immunohistochemical analysis and Western blot assay,respectively.HOXB9 was upregulated(P<0.0001),and AcK27-HOXB9(P=0.0023)was downregulated in patients with PDAC.HOXB9 promoted(P=0.0115),while AcK27-HOXB9(P=0.0279)inhibited PDAC progression.AcK27-HOXB9 predicted favorable outcome in patients with PDAC(P=0.0412).AcK27-HOXB9 also suppressed PDAC cell migration in a cell migration assay.The results of this study showed that HOXB9 promoted and AcK27-HOXB9 suppressed PDAC progression.The determination of ratio between HOXB9 and AcK27-HOXB9 exhibited potential diagnostic value in patients with PDAC.
文摘目的 HOXB9(homeobox B9)是HOX转录因子家族的一员,参与多种肿瘤的发生发展。本研究旨在探讨HOXB9基因在甲状腺乳头状癌中的表达,及沉默HOXB9基因对TPC-1细胞增殖与侵袭转移能力的影响。方法收集2016-06-01—2017-06-01潍坊市中医院收治的88例甲状腺癌根治术患者组织标本,每例组织标本包括癌组织和癌旁组织(距离肿瘤组织1~2cm并经病理确诊的非癌组织)。利用实时定量聚合酶链反应(quantitative real time polymerase chain reaction,qRT-PCR)检测甲状腺乳头状癌组织和癌周组织中HOXB9基因表达。构建HOXB9的siRNA瞬时转染细胞TPC-1,应用蛋白质印迹法检测siRNA的沉默效果。通过MTT试验检测细胞生长能力、平板克隆试验检测细胞增殖能力、Transwell小室侵袭与迁移试验检测TPC-1细胞的侵袭与迁移能力,应用qRT-PCR及蛋白质印迹法检测转移相关蛋白MMP2与CXCR4在mRNA及蛋白水平表达的变化。结果甲状腺乳头状癌组织中HOXB9表达水平0.3±0.2,明显低于癌旁组织的1±0.2,t=4.287,P=0.013。沉默HOXB9基因后,分别在24、48及72h检测TPC-1细胞生长能力,结果显示,siHOXB9-TPC-1试验组(F=120.527,P<0.001)的生长能力明显高于sicontrol-TPC-1对照组(F=27.574,P<0.001)。siHOXB9-TPC-1试验组的克隆数90±10.8,高于sicontrol-TPC-1对照组的35±3.4,P<0.001。siHOXB9-TPC-1试验组迁移细胞数目107±5.2,高于sicontrol-TPC-1对照组的53±3.8,P<0.001;siHOXB9-TPC-1试验组侵袭细胞数目80±4.7,高于sicontrol-TPC-1对照组相的46±3.2,P<0.001。在siHOXB9-TPC-1试验组中,沉默HOXB9后MMP2和CXCR4表达明显上调。结论HOXB9基因是一个甲状腺乳头状癌的肿瘤抑制因子,沉默HOXB9基因可促进甲状腺乳头状癌的生长增殖及侵袭转移,为靶向治疗甲状腺癌提供新的思路。
