Hoxa gene plays an essential role in the generation and development of the human female reproductive system. This gene is expressed in the Mullerian duct and the adult female reproductive system. Expression of Hoxa10 ...Hoxa gene plays an essential role in the generation and development of the human female reproductive system. This gene is expressed in the Mullerian duct and the adult female reproductive system. Expression of Hoxa10 dramatically increased during the midsecretory phase of the menstrual cycle, corresponding to the time of implantation. Female mice lacking Hoxa10 have a uterine factor defect that results in death of the preimplantation embryo and failure of implantation. These results suggest Hoxa10 may have an important function in implantation. Hoxa10 - deficient males exhibit cryptorchidism that lead to male infertility.展开更多
Homeobox A10 （HOXA 10） is a well-known transcription factor that plays an important role in directing endometrial differ- entiation and establishing the conditions required for implantation. Interestingly, the expre...Homeobox A10 （HOXA 10） is a well-known transcription factor that plays an important role in directing endometrial differ- entiation and establishing the conditions required for implantation. Interestingly, the expression level of HOXAIO may be associated with litter size. To study the effects of the porcine HOXAIO promoter fragment on the expression of HOXAIO gene in vivo, we generated a transgenic mouse model using pronuclear microinjection, and measured the expression of HOXAIO in the endometrium. There was no difference in the expression level of HOXAIO between transgenic and wild- type mice in the absence of hormone stimulation. However, following treatment with progesterone and estradiol benzoate, the expression level of HOXAIO was significantly increased in transgenic mice compared with that of wild-type mice. Fur- thermore, the litter size of transgenic females was larger than that of wild-type females （7.02±1.73 vs. 6.48＋1.85; P=0.14）. Moreover, the difference of litter size was greater in the later parities （7.33±1.62 vs. 6.37±2.02; P=0.08） compared with the first parity （6.76±1.81 vs. 6.61v1.67; P=0.77） between transgenic and wild-type mice. Therefore, our transgenic mouse model provides exciting insights regarding the actions of HOXAIO and its hormone-inducible promoter in vivo. The present study offers valuable proof of principle to develop transgenic pigs with a hormone-inducible promoter regulating HOXAIO to alter litter size.展开更多
文摘Hoxa gene plays an essential role in the generation and development of the human female reproductive system. This gene is expressed in the Mullerian duct and the adult female reproductive system. Expression of Hoxa10 dramatically increased during the midsecretory phase of the menstrual cycle, corresponding to the time of implantation. Female mice lacking Hoxa10 have a uterine factor defect that results in death of the preimplantation embryo and failure of implantation. These results suggest Hoxa10 may have an important function in implantation. Hoxa10 - deficient males exhibit cryptorchidism that lead to male infertility.
基金supported by the National Transgenic Project of China (2014ZX08006-005, 2014ZX0800950B)the Guangdong Science and Technology Project, China (2011A020102003)the Fundamental Research Funds for the Central Universities, China (2013PY050)
文摘Homeobox A10 （HOXA 10） is a well-known transcription factor that plays an important role in directing endometrial differ- entiation and establishing the conditions required for implantation. Interestingly, the expression level of HOXAIO may be associated with litter size. To study the effects of the porcine HOXAIO promoter fragment on the expression of HOXAIO gene in vivo, we generated a transgenic mouse model using pronuclear microinjection, and measured the expression of HOXAIO in the endometrium. There was no difference in the expression level of HOXAIO between transgenic and wild- type mice in the absence of hormone stimulation. However, following treatment with progesterone and estradiol benzoate, the expression level of HOXAIO was significantly increased in transgenic mice compared with that of wild-type mice. Fur- thermore, the litter size of transgenic females was larger than that of wild-type females （7.02±1.73 vs. 6.48＋1.85; P=0.14）. Moreover, the difference of litter size was greater in the later parities （7.33±1.62 vs. 6.37±2.02; P=0.08） compared with the first parity （6.76±1.81 vs. 6.61v1.67; P=0.77） between transgenic and wild-type mice. Therefore, our transgenic mouse model provides exciting insights regarding the actions of HOXAIO and its hormone-inducible promoter in vivo. The present study offers valuable proof of principle to develop transgenic pigs with a hormone-inducible promoter regulating HOXAIO to alter litter size.
