[Objective] The research aimed toexplore the method to obtain Hoxc8 pro- moter of Mongolian Sheep. [Method] Thermal asymmetric interlaced PCR was used to amplify the promoter sequence of Hoxc8 inMongolian Sheep. [Resu...[Objective] The research aimed toexplore the method to obtain Hoxc8 pro- moter of Mongolian Sheep. [Method] Thermal asymmetric interlaced PCR was used to amplify the promoter sequence of Hoxc8 inMongolian Sheep. [Result] The ob- tained sequence by usingthermal asymmetric interlaced PCRwas not ideal and the sequencing results were not matching to the known sequence. Though promoter se- quence of Hoxc8 in Mongolian Sheep was not obtained by thermal asymmetric in- terlaced PCR, but the results could provide references for the relevant studies in the future. [Conclusion] The research laid the foundation for further study on the methy- lation status Hoxc8 promoter in Mongolian Sheep.展开更多
目的探讨HOXC8在人肝门部胆管癌组织中的表达及其对预后的影响。方法采用免疫组化法检测49例肝门部胆管癌及对应癌旁正常组织中HOXC8蛋白的表达水平,并分析其与肝门部胆管癌患者临床病理特征及预后的关系。结果肝门部胆管癌组织中HOXC8...目的探讨HOXC8在人肝门部胆管癌组织中的表达及其对预后的影响。方法采用免疫组化法检测49例肝门部胆管癌及对应癌旁正常组织中HOXC8蛋白的表达水平,并分析其与肝门部胆管癌患者临床病理特征及预后的关系。结果肝门部胆管癌组织中HOXC8蛋白的阳性表达率明显高于癌旁正常组织(57.1% vs 42.9%,P<0.001)。HOXC8表达与肝门部胆管癌患者肿瘤浸润深度、淋巴结转移、TNM分期有关(P<0.05)。单因素生存分析显示HOXC8阳性表达的肝门部胆管癌患者总生存期明显较HOXC8阴性表达患者差(12个月 vs 46个月,P<0.001),多因素回归分析显示HOXC8是肝门部胆管癌患者的独立预后因素(P=0.039)。结论 HOXC8在肝门部胆管癌组织中高表达,且与不良预后有关,可能作为判断肝门部胆管癌患者预后的标志物及潜在治疗靶点。展开更多
目的研究HOXC8与头颈鳞癌的关系及判断预后的价值。方法利用公共数据库The Cancer Genome Atlas(TCGA)分析HOXC8与头颈鳞癌患者的生存关系及诊断价值。结果头颈鳞癌的HOXC8表达水平明显高于癌旁组织(P<0.05);在G3/G4组织分级和发生...目的研究HOXC8与头颈鳞癌的关系及判断预后的价值。方法利用公共数据库The Cancer Genome Atlas(TCGA)分析HOXC8与头颈鳞癌患者的生存关系及诊断价值。结果头颈鳞癌的HOXC8表达水平明显高于癌旁组织(P<0.05);在G3/G4组织分级和发生远处转移的头颈鳞癌中HOXC8表达水平明显升高(均P<0.05);年龄及HOXC8表达水平是头颈鳞癌患者预后的独立影响因素(均P<0.05);检测HOXC8表达水平的曲线下面积为0.937,敏感性和特异性分别是91.6%和85.0%。HOXC8启动子区域的10个CG位点的甲基化水平与HOXC8表达水平呈明显负相关(均P<0.05)。结论 HOXC8基因在头颈鳞癌的发生发展过程中发挥重要作用,HOXC8基因检测可能作为一个潜在的头颈鳞癌分子诊断标志物。展开更多
基金Supported by the Supporting Program of the"Eleventh Five-year Plan"for Sci&Tech Research of China(2006BDA13B08)National Natural Science Foundation of China(30960245)~~
文摘[Objective] The research aimed toexplore the method to obtain Hoxc8 pro- moter of Mongolian Sheep. [Method] Thermal asymmetric interlaced PCR was used to amplify the promoter sequence of Hoxc8 inMongolian Sheep. [Result] The ob- tained sequence by usingthermal asymmetric interlaced PCRwas not ideal and the sequencing results were not matching to the known sequence. Though promoter se- quence of Hoxc8 in Mongolian Sheep was not obtained by thermal asymmetric in- terlaced PCR, but the results could provide references for the relevant studies in the future. [Conclusion] The research laid the foundation for further study on the methy- lation status Hoxc8 promoter in Mongolian Sheep.
文摘目的探讨HOXC8在人肝门部胆管癌组织中的表达及其对预后的影响。方法采用免疫组化法检测49例肝门部胆管癌及对应癌旁正常组织中HOXC8蛋白的表达水平,并分析其与肝门部胆管癌患者临床病理特征及预后的关系。结果肝门部胆管癌组织中HOXC8蛋白的阳性表达率明显高于癌旁正常组织(57.1% vs 42.9%,P<0.001)。HOXC8表达与肝门部胆管癌患者肿瘤浸润深度、淋巴结转移、TNM分期有关(P<0.05)。单因素生存分析显示HOXC8阳性表达的肝门部胆管癌患者总生存期明显较HOXC8阴性表达患者差(12个月 vs 46个月,P<0.001),多因素回归分析显示HOXC8是肝门部胆管癌患者的独立预后因素(P=0.039)。结论 HOXC8在肝门部胆管癌组织中高表达,且与不良预后有关,可能作为判断肝门部胆管癌患者预后的标志物及潜在治疗靶点。
文摘目的研究HOXC8与头颈鳞癌的关系及判断预后的价值。方法利用公共数据库The Cancer Genome Atlas(TCGA)分析HOXC8与头颈鳞癌患者的生存关系及诊断价值。结果头颈鳞癌的HOXC8表达水平明显高于癌旁组织(P<0.05);在G3/G4组织分级和发生远处转移的头颈鳞癌中HOXC8表达水平明显升高(均P<0.05);年龄及HOXC8表达水平是头颈鳞癌患者预后的独立影响因素(均P<0.05);检测HOXC8表达水平的曲线下面积为0.937,敏感性和特异性分别是91.6%和85.0%。HOXC8启动子区域的10个CG位点的甲基化水平与HOXC8表达水平呈明显负相关(均P<0.05)。结论 HOXC8基因在头颈鳞癌的发生发展过程中发挥重要作用,HOXC8基因检测可能作为一个潜在的头颈鳞癌分子诊断标志物。
