The present study established a rat model of focal brain ischemia by occlusion of the middle cerebral artery covered with FeCl3, and investigated the protective effect of 3＇-methoxy-puerarin. Hippocampal and cortical...The present study established a rat model of focal brain ischemia by occlusion of the middle cerebral artery covered with FeCl3, and investigated the protective effect of 3＇-methoxy-puerarin. Hippocampal and cortical c-fos gene expression was determined using in situ hybridization. Results showed that 3＇-methoxy-puerarin reduced neurological deficit scores, cerebral infarcted zone and water content of brain tissues, dramatically increased the activity of catalase and glutathione peroxidase in the ischemia zone of the hippocampus, increased the activity of catalase in the cortex, decreased lipid peroxide and lactic acid contents in the hippocampus and cerebral cortex, and down-regulated c-fos gene expression in brain ischemic rats. Results demonstrated that 3＇-methoxy-puerarin exhibited cerebroprotective effects against focal brain ischemia, which involved c-fos gene expression.展开更多
目的 采用HPLC法多指标成分定量测定联合正交偏最小二乘-判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)及熵权优劣解距离法(entropy weight technique for order preference by similarity to ideal solut...目的 采用HPLC法多指标成分定量测定联合正交偏最小二乘-判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)及熵权优劣解距离法(entropy weight technique for order preference by similarity to ideal solution,EWTOPSIS)对不同厂家降糖通脉片(Jiangtang Tongmai Tablets,JTT)质量进行综合评价,为科学评价JTT质量提供参考。方法 收集3个厂家15批JTT样品,采用HPLC法同时测定JTT中3’-羟基葛根素、葛根素、3’-甲氧基葛根素、太子参环肽B、哈巴苷、哈巴俄苷、二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮Ⅱ_(A)的含量,运用SPSS26.0和SIMCA14.1统计软件进行层次聚类分析(hierarchical cluster analysis,HCA)、主成分分析(principal component analysis,PCA)和OPLS-DA,建立JTT HCA、PCA和OPLS-DA模型,获取得分图和载荷图及变量重要性投影(variable importance in projection,VIP)值,筛选并分析影响JTT质量差异的主要物质基础,并运用EW-TOPSIS法对不同厂家JTT进行质量优劣性评价。结果 3’-羟基葛根素、葛根素、3’-甲氧基葛根素、太子参环肽B、哈巴苷、哈巴俄苷、二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮Ⅱ_(A)分别在5.97~298.50、14.45~722.50、4.89~244.50、0.58~29.00、6.55~327.50、2.46~123.00、1.39~69.50、3.56~178.00、4.25~212.50和7.97~398.50μg/mL(r≥0.999 1)线性关系良好,平均加样回收率(n=9)分别为99.53%、100.10%、98.95%、96.97%、100.03%、98.84%、97.06%、98.37%、99.31%、99.18%,RSD分别为0.71%、0.63%、0.98%、1.47%、0.79%、1.30%、1.26%、1.18%、1.39%、1.55%。化学计量学分析结果显示15批JTT样品聚为3类,同一厂家JTT样品聚为一类,不同生产厂家样品质量存在一定差异;葛根素、丹参酮Ⅰ、哈巴苷和丹参酮Ⅱ_(A)是影响JTT样品质量的主要潜在标志物;EW-TOPSIS法可用于不同厂家JTT质量优劣的评价。结论 建立的HPLC多指标成分定量测定方法操作便捷、结果准确,结合HCA、PCA、OPLS-DA及EW-TOPSIS法可用于JTT质量的综合评价。展开更多
文摘The present study established a rat model of focal brain ischemia by occlusion of the middle cerebral artery covered with FeCl3, and investigated the protective effect of 3＇-methoxy-puerarin. Hippocampal and cortical c-fos gene expression was determined using in situ hybridization. Results showed that 3＇-methoxy-puerarin reduced neurological deficit scores, cerebral infarcted zone and water content of brain tissues, dramatically increased the activity of catalase and glutathione peroxidase in the ischemia zone of the hippocampus, increased the activity of catalase in the cortex, decreased lipid peroxide and lactic acid contents in the hippocampus and cerebral cortex, and down-regulated c-fos gene expression in brain ischemic rats. Results demonstrated that 3＇-methoxy-puerarin exhibited cerebroprotective effects against focal brain ischemia, which involved c-fos gene expression.
文摘目的 采用HPLC法多指标成分定量测定联合正交偏最小二乘-判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)及熵权优劣解距离法(entropy weight technique for order preference by similarity to ideal solution,EWTOPSIS)对不同厂家降糖通脉片(Jiangtang Tongmai Tablets,JTT)质量进行综合评价,为科学评价JTT质量提供参考。方法 收集3个厂家15批JTT样品,采用HPLC法同时测定JTT中3’-羟基葛根素、葛根素、3’-甲氧基葛根素、太子参环肽B、哈巴苷、哈巴俄苷、二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮Ⅱ_(A)的含量,运用SPSS26.0和SIMCA14.1统计软件进行层次聚类分析(hierarchical cluster analysis,HCA)、主成分分析(principal component analysis,PCA)和OPLS-DA,建立JTT HCA、PCA和OPLS-DA模型,获取得分图和载荷图及变量重要性投影(variable importance in projection,VIP)值,筛选并分析影响JTT质量差异的主要物质基础,并运用EW-TOPSIS法对不同厂家JTT进行质量优劣性评价。结果 3’-羟基葛根素、葛根素、3’-甲氧基葛根素、太子参环肽B、哈巴苷、哈巴俄苷、二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮Ⅱ_(A)分别在5.97~298.50、14.45~722.50、4.89~244.50、0.58~29.00、6.55~327.50、2.46~123.00、1.39~69.50、3.56~178.00、4.25~212.50和7.97~398.50μg/mL(r≥0.999 1)线性关系良好,平均加样回收率(n=9)分别为99.53%、100.10%、98.95%、96.97%、100.03%、98.84%、97.06%、98.37%、99.31%、99.18%,RSD分别为0.71%、0.63%、0.98%、1.47%、0.79%、1.30%、1.26%、1.18%、1.39%、1.55%。化学计量学分析结果显示15批JTT样品聚为3类,同一厂家JTT样品聚为一类,不同生产厂家样品质量存在一定差异;葛根素、丹参酮Ⅰ、哈巴苷和丹参酮Ⅱ_(A)是影响JTT样品质量的主要潜在标志物;EW-TOPSIS法可用于不同厂家JTT质量优劣的评价。结论 建立的HPLC多指标成分定量测定方法操作便捷、结果准确,结合HCA、PCA、OPLS-DA及EW-TOPSIS法可用于JTT质量的综合评价。
