高效液相荧光法测定养殖水产中四种氟喹诺酮类药物残留

建立了同时检测人工养殖水产品中环丙沙星、恩诺沙星、沙拉沙星、达氟沙星4种氟喹诺酮类药物的HPLC-FLC检测方法。水产品用流动相溶液提取,HLB固相萃取柱净化萃取,采用乙腈-甲酸水溶液作为流动相,梯度洗脱,荧光检测器检测。该法测得4种FQs在对虾肉、大闸蟹肉、黑鱼肉中的回收率分别为79.5%~99.6%、76.8%~101.4%、77.9%~103.2%。该方法用来测定水产品中氟喹诺酮类药物残留灵敏、快速、准确,适用于人工养殖水产中氟喹诺酮类抗生素的检测。

Antifungal activity of total and fractionated phenolic extracts from two wild edible herbs

The chemical characterization of phenolic extracts of salad burnet (Sanguisorba minor Scop. s.l.) and broomrape (Orobanche crenata Forsk.) was performed. Active fractions were separated and characterized and, in the case of O. crenata, two phenolic compounds, verbascoside and is overbascoside, were quantitatively extracted, purified and characterized. These compounds proved to be effective in reducing the growth of some important fungi responsible for the decay of fresh fruit and vegetables in postharvest. Verbascoside showed significant activity against Penicillium italicum, fairly inhibited Monilinia laxa, P. expansum, and Aspergillus carbonarius, and showed little activity against Botrytis cinerea, M. fructicola and P. digitatum. Isoverbascoside completely inhibited B. cinerea, P. digitatum, P. italicum, and P. expansum;it fairly inhibited M. laxa and A. carbonarius;while it showed to be less effective against M. fructicola. The obtained results support the possible use of some wild edible plants as a source of phenolic substances for the postharvest biological control of fresh fruit and vegetable rots.

疳积合剂等制剂质量标准提高的研究

目的通过薄层色谱鉴别(TLC)、高效液相色谱法(HPLC)的实验方法,为医院中药制剂质量标准的提高奠定基础。方法对本院制剂-疳积合剂中威灵仙、使君子,补血复明合剂中何首乌进行薄层鉴别,并通过高效液相色谱法对多动安口服液有效成分芍药苷进行含量测定。结果疳积合剂中威灵仙和补血复明合剂中何首乌在薄层色谱上具有鉴别特征,斑点清晰无干扰,而使君子在薄层色谱中无清晰斑点,还有待研究;初步摸索出HPLC测定多动安口服液中芍药苷的含量方法。结论薄层色谱鉴别方法可用于疳积合剂中威灵仙和补血复明合剂中何首乌的质量控制;高效液相色谱法简便、准确,能精确反映有效成分的含量。