Analysis of Multi-ingredient Food Supplements by Fingerprint HPTLC Approach

The increase of import/export of every kind of herbal products calls urgently for adequate controls. Analysis of herbal food supplement （botanicals） is a difficult task, like in the composition determination of a multi-ingredient product, where several botanical drugs were used. Actually, this is an important argument in consideration of health security. The authors reported the results of an analytical approach based on HPTLC （high performance thin layer chromatography） fingerprints comparison and tailored to determine the composition of marketed multi-ingredient botanicals. The method gave positive data in case of the presence of 3-5 species, whereas difficulties were recorded when the number of plants is increased.

救必应及其常用同属药用植物高效薄层指纹图谱研究

目的以丁香苷、具栖冬青苷和铁冬青酸为对照建立救必应药材主要有效成分的高效薄层指纹图谱,分析比较取自救必应不同部位和不同横切面厚度的茎皮的指纹图谱,并与较易与之混淆的同属药用植物毛冬青及岗梅的指纹图谱进行比较。方法采用硅胶GF254高效预制薄层板,以三氯甲烷-乙酸乙酯-甲醇-水-甲酸(体积比8∶24∶5∶2∶2)为展开剂,10%硫酸乙醇溶液显色后获得薄层荧光色谱指纹图谱,经指纹图谱系统解决方案软件(ChromafingerTM)生成共有模式,并进行相似度与主成分分析。结果救必应薄层荧光色谱指纹图谱由11条特征荧光斑条组成。将荧光色谱图像与轮廓扫描图谱结合分析,建立了救必应指纹图谱模式。结论救必应不同药用部位之间、不同厚度药材之间及与其同属药用植物之间指纹图谱差异均较大,应加以区别,不可混用。

水样中农药“灭扫利”的色谱分析

本文研究了从水样中提取“灭扫利”的方法,并采用GC/FID法,HPTLC法进行了定性、定量检测。GC/FID法线性范围为0～30ng/μl,HPTLC法检测灵敏度为2μg,回收率可达88％,利用该法进行案件检验,取得了满意的结果。