Objective To explore the relationship between heat-shock protein 70-2 gene +1267A/G polymorphism(HSP70-2 gene +1267A/G polymorphism) and serum level of high sensitivity C-reactive protein(Hs-CRP) in patients with coro...Objective To explore the relationship between heat-shock protein 70-2 gene +1267A/G polymorphism(HSP70-2 gene +1267A/G polymorphism) and serum level of high sensitivity C-reactive protein(Hs-CRP) in patients with coronary artery disease(CAD).Methods Serum Hs-CRP level and HSP70-2 gene +1267A/G polymorphism were detected in 185 patients with CAD.HSP70-2 gene +1267A/G polymorphism was screened by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Results The genotype distribution of the HSP70-2 gene +1267A/G polymorphism was as follows: AA was in 24 of 185 patients(12.97%),AG in 93 of 185 patients(50.27%) and GG in 68 of 185 patients(36.76%).Patients with GG、AG subtype had higher Hs-CRP levels compared with carriers of the AA subtype(4.5±1.8mg/L,3.2±1.2mg/L and 2.7±1.1mg/L,respectively,P<0.05).Among patients who had Hs-CRP >3mg/L,GG,and AG gene subtypes had higher percentage than A allele homozygotes(92.6%,74.2% and 45.8%,respectively.χ2=17.75,28.04,P<0.05).Conclusions This study indicated G allele of HSP70-2 gene +1267A/G polymorphism may be an independent risk factor in systemic inflammation with stable CAD.展开更多
目的:研究镍铬合金修复体对口腔牙龈组织的影响。方法:本文运用免疫组化染色SP(Streptavidin-peroxidase)法检测COX-2(cyclooxygenase-2)、HSP60(heat shock protein 60)、HSP70(heat shock protein 70)在镍铬合金修复体患者牙龈组织和...目的:研究镍铬合金修复体对口腔牙龈组织的影响。方法:本文运用免疫组化染色SP(Streptavidin-peroxidase)法检测COX-2(cyclooxygenase-2)、HSP60(heat shock protein 60)、HSP70(heat shock protein 70)在镍铬合金修复体患者牙龈组织和正常牙龈组织中的表达情况。经患者同意,从临床采集镍铬合金修复体患者牙龈30例作为实验组,采集口内无任何修复体患者的正常牙龈26例,将实验组中同一患者的牙龈组织分成3份,用免疫组织化学技术分别作COX-2、HSP60、HSP70的检测。每种指标检测30例,对照组同实验组。结果进行统计学分析。结果:HSP60和HSP70在镍铬合金修复体患者牙龈组织中表达明显高于对照组正常牙龈组织(P<0.05)。COX-2在实验组中的表达亦高于对照组(P<0.05)。结论:镍铬合金修复体在口腔中游离出的金属离子对牙龈组织具有致敏性和致炎性,但是否具有致癌性还有待研究。展开更多
文摘Objective To explore the relationship between heat-shock protein 70-2 gene +1267A/G polymorphism(HSP70-2 gene +1267A/G polymorphism) and serum level of high sensitivity C-reactive protein(Hs-CRP) in patients with coronary artery disease(CAD).Methods Serum Hs-CRP level and HSP70-2 gene +1267A/G polymorphism were detected in 185 patients with CAD.HSP70-2 gene +1267A/G polymorphism was screened by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Results The genotype distribution of the HSP70-2 gene +1267A/G polymorphism was as follows: AA was in 24 of 185 patients(12.97%),AG in 93 of 185 patients(50.27%) and GG in 68 of 185 patients(36.76%).Patients with GG、AG subtype had higher Hs-CRP levels compared with carriers of the AA subtype(4.5±1.8mg/L,3.2±1.2mg/L and 2.7±1.1mg/L,respectively,P<0.05).Among patients who had Hs-CRP >3mg/L,GG,and AG gene subtypes had higher percentage than A allele homozygotes(92.6%,74.2% and 45.8%,respectively.χ2=17.75,28.04,P<0.05).Conclusions This study indicated G allele of HSP70-2 gene +1267A/G polymorphism may be an independent risk factor in systemic inflammation with stable CAD.
文摘目的:研究镍铬合金修复体对口腔牙龈组织的影响。方法:本文运用免疫组化染色SP(Streptavidin-peroxidase)法检测COX-2(cyclooxygenase-2)、HSP60(heat shock protein 60)、HSP70(heat shock protein 70)在镍铬合金修复体患者牙龈组织和正常牙龈组织中的表达情况。经患者同意,从临床采集镍铬合金修复体患者牙龈30例作为实验组,采集口内无任何修复体患者的正常牙龈26例,将实验组中同一患者的牙龈组织分成3份,用免疫组织化学技术分别作COX-2、HSP60、HSP70的检测。每种指标检测30例,对照组同实验组。结果进行统计学分析。结果:HSP60和HSP70在镍铬合金修复体患者牙龈组织中表达明显高于对照组正常牙龈组织(P<0.05)。COX-2在实验组中的表达亦高于对照组(P<0.05)。结论:镍铬合金修复体在口腔中游离出的金属离子对牙龈组织具有致敏性和致炎性,但是否具有致癌性还有待研究。
