Purpose:To evaluate the possibility of HSV-1 corneal latency by in situ nucleic acid hybridization in animal models.Methods:20 normal New Zealand White(NEW)rabbits were used,14of them were inoculated bilaterally with ...Purpose:To evaluate the possibility of HSV-1 corneal latency by in situ nucleic acid hybridization in animal models.Methods:20 normal New Zealand White(NEW)rabbits were used,14of them were inoculated bilaterally with 3×10PFU/ml of McKrae strain HSV-1by in-trastromal injection,22/28eyes developed typical herpes simplex keratitis(HSK) diseases.At 60day postoperation(PI),4latent corneas were transplanted to one eye of 4noninfected NZW rabbits and removed2weeks PI,Corneas at all time intervals of infection and two weeks after PKPwere detected for presence of HSV-1antigen and nucleic acid sequences by using clonal IgGHSV-1antibody and biotinylated HSV-1DNAprobe individually.Results:The results showed that the HSV-1DNA sequences were retained with-in the corneal epithelium and anterior stromal keratocytes during acute diseases,while the corneas during latent infection and postoperation,the HSV-1DNAse-quences were retained only within the stromal layer with negative HSV-1antigne staining.Conclusions:These results strongly suggest that the cornea may be capable of harburing latent HSV-1.Eye Science 1995;11:117-119.展开更多
文摘Purpose:To evaluate the possibility of HSV-1 corneal latency by in situ nucleic acid hybridization in animal models.Methods:20 normal New Zealand White(NEW)rabbits were used,14of them were inoculated bilaterally with 3×10PFU/ml of McKrae strain HSV-1by in-trastromal injection,22/28eyes developed typical herpes simplex keratitis(HSK) diseases.At 60day postoperation(PI),4latent corneas were transplanted to one eye of 4noninfected NZW rabbits and removed2weeks PI,Corneas at all time intervals of infection and two weeks after PKPwere detected for presence of HSV-1antigen and nucleic acid sequences by using clonal IgGHSV-1antibody and biotinylated HSV-1DNAprobe individually.Results:The results showed that the HSV-1DNA sequences were retained with-in the corneal epithelium and anterior stromal keratocytes during acute diseases,while the corneas during latent infection and postoperation,the HSV-1DNAse-quences were retained only within the stromal layer with negative HSV-1antigne staining.Conclusions:These results strongly suggest that the cornea may be capable of harburing latent HSV-1.Eye Science 1995;11:117-119.
