目的分析讨论血清白色念珠菌菌丝壁蛋白1(hyphal wall protein 1,HWP1)检测在儿童皮肤念珠菌病诊断中的灵敏度和特异性等诊断效力。方法选取本院2014年1月至2016年12月期间收治的86例儿童皮肤病患者进行研究,所有患儿均接受血清HWP1检测...目的分析讨论血清白色念珠菌菌丝壁蛋白1(hyphal wall protein 1,HWP1)检测在儿童皮肤念珠菌病诊断中的灵敏度和特异性等诊断效力。方法选取本院2014年1月至2016年12月期间收治的86例儿童皮肤病患者进行研究,所有患儿均接受血清HWP1检测,将检测结果与金标准诊断结果对比,分析血清HWP1检测诊断儿童皮肤病念珠菌感染的灵敏度、特异性、阳性诊断价值和阴性诊断价值。结果本研究中86例患儿经组织培养确诊为念珠菌感染的患儿41例,非念珠菌感染患儿45例,两性间血清HWP1抗体阳性率相比,差异无统计学意义(P>0.05);念珠菌感染患儿血清HWP1抗体阳性率(85.37%)显著高于非念珠菌感染患儿(6.67%),差异有统计学意义(P<0.05);血清HWP1检测诊断灵敏度85.37%,特异性93.33%,阳性预测值92.11%,阴性预测值87.50%,ROC曲线下面积为0.896。结论血清HWP1能够为儿童皮肤病念珠菌感染筛查和鉴别诊断提供高价值信息,其灵敏度优良,特异性非常高,值得临床推荐。展开更多
目的：研究临床致病白念珠菌交配型基因MTL纯合子与杂合子的流行病学特点,分析其与外阴阴道念珠菌病患者的临床特征、白念珠菌致病力及毒力的关系。方法：通过普通PCR对临床外阴阴道念珠菌病患者阴道分泌物分离的致病白念珠菌MTL基因进...目的：研究临床致病白念珠菌交配型基因MTL纯合子与杂合子的流行病学特点,分析其与外阴阴道念珠菌病患者的临床特征、白念珠菌致病力及毒力的关系。方法：通过普通PCR对临床外阴阴道念珠菌病患者阴道分泌物分离的致病白念珠菌MTL基因进行检测,应用实时荧光定量PCR反应分析MTL纯合子与杂合子中毒力相关基因ASL3、SAP1和HWP1基因的表达,并采用Rosco药片扩散法对白念珠菌进行体外药物敏感试验。结果：1338株白念珠菌中,1330株为MTLa/α杂合子,8株为MTL纯合子（7株MTLa/a纯合子,1株MTLα/α纯合子）。8株MTL纯合子所致外阴阴道念珠菌病患者中6例患者达到真菌学治愈。与杂合子相比,白念珠菌MTL纯合子对唑类药物耐药性较高（0~75%vs 0~2.7%,P〈0.05）。毒力相关基因ASL3在两者中的表达无明显统计学差异,SAP1在MTL杂合子中表达稍高（1.02±0.68 vs 0.83±0.31,P〉0.05）,而HWP1在杂合子中表达明显增高（1.71±1.42 vs 1.20±0.62,P〈0.05）。结论：外阴阴道念珠菌病白念珠菌MTL纯合子在外阴阴道念珠菌病患者中流行率较低;与杂合子相比,MTL纯合子耐药性增加;而毒力相关基因HWP1、SAP1和ASL3在纯合子中表达降低,这与其临床特征相符。展开更多
Knowledge of simulated microgravity （SMG）-induced changes in the pathogenicity of microorganisms is important for success of long-term spaceflight. In a previous study using the high aspect ratio vessel bioreactor, ...Knowledge of simulated microgravity （SMG）-induced changes in the pathogenicity of microorganisms is important for success of long-term spaceflight. In a previous study using the high aspect ratio vessel bioreactor, we showed that the yeast species Saccharomyces cerevisiae underwent a significant phenotypic response when grown in modeled microgravity, which was reflected in the analysis of gene expression profiles. In this study, we establish that Candida albicans responds to SMG in a similar fashion, demonstrating that there is a conserved response among yeast to this environmental stress. We also report that the growth of C. albicans in SMG results in a morphogenic switch that is consistent with enhanced pathogenicity. Specifically, we observed an increase in filamentous forms of the organism and accompanying changes in the expression of two genes associated with the yeasthyphal transition. The morphological response may have significant implications for astronauts＇ safety, as the fungal pathogen may become more virulent during spaceflight.展开更多
文摘目的分析讨论血清白色念珠菌菌丝壁蛋白1(hyphal wall protein 1,HWP1)检测在儿童皮肤念珠菌病诊断中的灵敏度和特异性等诊断效力。方法选取本院2014年1月至2016年12月期间收治的86例儿童皮肤病患者进行研究,所有患儿均接受血清HWP1检测,将检测结果与金标准诊断结果对比,分析血清HWP1检测诊断儿童皮肤病念珠菌感染的灵敏度、特异性、阳性诊断价值和阴性诊断价值。结果本研究中86例患儿经组织培养确诊为念珠菌感染的患儿41例,非念珠菌感染患儿45例,两性间血清HWP1抗体阳性率相比,差异无统计学意义(P>0.05);念珠菌感染患儿血清HWP1抗体阳性率(85.37%)显著高于非念珠菌感染患儿(6.67%),差异有统计学意义(P<0.05);血清HWP1检测诊断灵敏度85.37%,特异性93.33%,阳性预测值92.11%,阴性预测值87.50%,ROC曲线下面积为0.896。结论血清HWP1能够为儿童皮肤病念珠菌感染筛查和鉴别诊断提供高价值信息,其灵敏度优良,特异性非常高,值得临床推荐。
文摘目的：研究临床致病白念珠菌交配型基因MTL纯合子与杂合子的流行病学特点,分析其与外阴阴道念珠菌病患者的临床特征、白念珠菌致病力及毒力的关系。方法：通过普通PCR对临床外阴阴道念珠菌病患者阴道分泌物分离的致病白念珠菌MTL基因进行检测,应用实时荧光定量PCR反应分析MTL纯合子与杂合子中毒力相关基因ASL3、SAP1和HWP1基因的表达,并采用Rosco药片扩散法对白念珠菌进行体外药物敏感试验。结果：1338株白念珠菌中,1330株为MTLa/α杂合子,8株为MTL纯合子（7株MTLa/a纯合子,1株MTLα/α纯合子）。8株MTL纯合子所致外阴阴道念珠菌病患者中6例患者达到真菌学治愈。与杂合子相比,白念珠菌MTL纯合子对唑类药物耐药性较高（0~75%vs 0~2.7%,P〈0.05）。毒力相关基因ASL3在两者中的表达无明显统计学差异,SAP1在MTL杂合子中表达稍高（1.02±0.68 vs 0.83±0.31,P〉0.05）,而HWP1在杂合子中表达明显增高（1.71±1.42 vs 1.20±0.62,P〈0.05）。结论：外阴阴道念珠菌病白念珠菌MTL纯合子在外阴阴道念珠菌病患者中流行率较低;与杂合子相比,MTL纯合子耐药性增加;而毒力相关基因HWP1、SAP1和ASL3在纯合子中表达降低,这与其临床特征相符。
文摘Knowledge of simulated microgravity （SMG）-induced changes in the pathogenicity of microorganisms is important for success of long-term spaceflight. In a previous study using the high aspect ratio vessel bioreactor, we showed that the yeast species Saccharomyces cerevisiae underwent a significant phenotypic response when grown in modeled microgravity, which was reflected in the analysis of gene expression profiles. In this study, we establish that Candida albicans responds to SMG in a similar fashion, demonstrating that there is a conserved response among yeast to this environmental stress. We also report that the growth of C. albicans in SMG results in a morphogenic switch that is consistent with enhanced pathogenicity. Specifically, we observed an increase in filamentous forms of the organism and accompanying changes in the expression of two genes associated with the yeasthyphal transition. The morphological response may have significant implications for astronauts＇ safety, as the fungal pathogen may become more virulent during spaceflight.
