Serum tumor markers (carcinoembryonic antigen, carbohydrate antigen 19-9, carbohydrate antigen 72-4, carbohydrate antigen 24-2, ferritin) and gastric cancer prognosis correlation

BACKGROUND Gastric cancer is a kind of malignant tumor which is prevalent all over the world.Although some progress has been made in the treatment of gastric cancer,its prognosis is still not optimistic,so it is of great significance to find reliable prog-nostic indicators to guide the treatment and management of patients with gastric cancer.AIM To explore the relationship between serum levels of five biomarkers[carcinoem-bryonic antigen(CEA),carbohydrate antigen(CA)19-9,CA72-4,CA24-2,and ferritin]and prognosis in patients with gastric cancer.METHODS This study included 200 patients with gastric adenocarcinoma,and conducted an in-depth analysis of their baseline characteristics,relationship between tumor markers and staging,and prognosis.The study found that CA19-9 has a signi-ficant correlation with tumor stage,the average levels of CA24-2,CEA,CA72-4 and ferritin were slightly increased disregarding the stage of tumor.Survival analysis showed that increases in CEA,CA19-9,CA24-2,and ferritin were all associated with shortened overall survival of patients.Further multivariate ana-lysis revealed that elevated serum CA72-4 levels were an inde-pendent adverse prognostic factor.RESULTS This study reveals that there is a significant correlation between the expression levels of serum tumor markers CEA,CA19-9,CA72-4,CA24-2 and ferritin in patients with gastric cancer and prognosis,and can be used as important indicators for prognostic evaluation of gastric cancer.In particular,markers that appear abnormally elevated initially may help identify gastric cancer patients with poor prognosis.CONCLUSION Serum CEA and CA19-9 play an important role in the prognosis assessment of gastric cancer,and are effective tools to guide clinical practice and optimize individualized treatment strategies for gastric cancer patients.

11th INTERNATIONAL CONFERENCE ON HYDRODYNAMICS19-24 October 2014 SINGAPORE First Announcement and Call for Paper

CONFERENCE THEMES The overall aim of the ICHD Conference is to provide a forum for participants from around the world to review, discuss and present the latest developments in the broad discipline of hydrodynamics and fluid mechanics.

24-亚甲基胆甾-5-烯-3β,19-二醇的合成

从豆甾醇出发 ,通过 1 0步反应 ,合成得到具有显著细胞毒活性的 2 4-亚甲基胆甾 -5 -烯 -3β,1 9-二醇( 1 ) ,总产率为 1 6% .目标产物 1的熔点、 [α]值和波谱数据与天然产物一致 .化合物 1的合成是首次报道 ,它对人体鼻咽癌 ( CN2 )和胃癌 ( Mgc80 3 )细胞有显著抑制活性 .

3-乙酰氧基胆甾-5-烯-19-羟基-24-酮的制备与表征

通过3β-乙酰氧基-5-烯胆甾-24-酮(2)与N-溴代乙酰胺(NBA)反应得到3β-乙酰氧基-5α-溴-6β羟基胆甾-24-酮(3),(3)通过光环化反应后不经分离直接通过锌粉/乙酸水解得到52％产率的化合物3-乙酰氧基胆甾-5-烯-19羟基-24-酮(5)。缩短了合成天然多羟基甾醇24-亚甲基胆甾-5-烯-3β,7β,19-三醇(1)的时间并减少中间过程的物损耗,进一步提高了反应产率。

老年胆管细胞癌患者外周血糖蛋白抗原19-9与24-2水平变化及其临床意义

目的探讨血清糖蛋白抗原(CA)19-9与CA24-2水平动态表达在老年胆管细胞癌患者诊断、预后及复发中的临床意义。方法采用电化学发光免疫分析法,分别以年龄匹配的20例胆系良性疾病及20例正常人作对照,对26例老年胆管细胞癌患者外周血CA19-9与CA24-2水平进行检测。结果胆管细胞癌组外周血CA19-9与CA24-2表达阳性率分别为76.9%,69.2%,明显高于胆系良性疾病组(5.0%,10.0%)及正常对照组(0.0%,5.0%)(均P<0.01);CA19-9、CA24-2的均值分别[(1 571.82±291.75)、(181.69±71.80)U/ml]明显高于胆系良性疾病组[(28.52±10.61)、(16.26±11.50)U/ml]及正常对照组[(22.53±6.42)、(10.68±5.87)U/ml](P<0.01)。CA19-9与CA24-2联合检测阳性率(88.5%)明显高于单一检测(P<0.05)。临床影像学检查提示胆管细胞癌占位性病变的,同时CA19-9、CA24-2联合检测明显升高的患者术后经病理证实均为胆管细胞癌。未复发胆管细胞癌组术后1 w CA19-9、CA24-2水平较术前均明显下降(P<0.05);而术后复发组术后1 w血清CA19-9、CA24-2较术前亦明显下降(P<0.05);术后4 w下降最明显,术后12 w水平回升;姑息手术组手术前后CA19-9、CA24-2水平无显著变化(P<0.05)。结论动态监测血清CA19-9、CA24-2水平变化对于老年胆管细胞癌的临床诊断及术后复发的判断均有重要价值。

结直肠癌患者血清循环肿瘤细胞、糖类抗原24-2、糖类抗原19-9水平及其与临床病理特征关系

目的:检测结直肠癌患者血清循环肿瘤细胞(CTC)、糖类抗原24-2(CA_(242))和糖类抗原19-9(CA_(199))水平,探讨它们与直肠癌患者不同临床病理特征的关系。方法:收集106例结直肠癌患者作为结直肠癌组,收集结直肠腺瘤患者100例作为结直肠腺瘤组,收集同期健康体检者100例作为对照组。采用实时荧光定量聚合酶链反应(qRT-PCR)检测CTC水平。采用全自动免疫分析仪检测CA_(242)和CA_(199)水平。比较三组研究对象CTC、CA_(242)、CA_(199)水平。比较不同临床病理特征结直肠癌患者CTC、CA_(242)、CA_(199)水平。分析CTC、CA_(242)、CA_(199)与结直肠癌患者不同临床病理特征的相关性。结果:结直肠癌组和结直肠腺瘤组患者CTC、CA_(242)、CA_(199)水平明显高于对照组,且结直肠癌组CTC、CA_(242)、CA_(199)水平明显高于结直肠腺瘤组(均P<0.05)。CTC、CA_(242)、CA_(199)水平在TNM分期为Ⅲ-Ⅳ期、分化程度为低分化、有淋巴结转移和肝转移的结直肠癌患者中明显升高(均P<0.05)。Spearman相关性分析结果显示,CTC、CA_(242)、CA_(199)水平与TNM分期、分化程度、淋巴结转移、肝转移密切相关(均P<0.05)。结论:CTC、CA_(242)、CA_(199)水平在结直肠癌患者中明显升高,且与结直肠癌患者临床病理特征密切相关。

血清CA19-9、CA24-2、CEA联合检测在胰癌诊断中的价值

对１１２例ＣＡ１９－９异常的病例联合检测ＣＡ２４－２和ＣＥＡ，其中胰腺癌组６４例中，ＣＡ２４－２异常者５４例，阳性率８４．４％；ＣＥＡ异常者４５例，阳性率７０．３％。ＣＡ１９－９、ＣＡ２４－２、ＣＥＡ３项中２项阳性者６３例，阳性率９８．４％。与非胰腺癌组比较Ｐ＜０．０１。结果表明，联合检测ＣＡ１９－９、ＣＡ２４－２和ＣＥＡ可显著提高胰腺癌诊断的特异性。

女性血清CA19-9,CA24-2水平与雌二醇变化的关系

目的:探讨女性生理周期血清CA19-9,CA24-2水平与雌二醇之间的关系。方法:检测150例处于不同生理周期的女性血清CA19-9、CA24-2和雌二醇水平,分析三者之间的关系,同时以150例男性为对照。结果:女性血清CA19-9和CA24-2水平明显高于对照组(t=4.848,P<0.001;t=8.11,P<0.001)。女性血清CA19-9与CA24-2的阳性率也明显高于男性(χ2=6.621,P=0.010 1;χ2=8.219,P=0.004 1)。不同生理周期(经期、卵泡期、黄体期)的女性血清CA19-9和CA24-2水平之间差异有统计学意义(F=10.53,P<0.001;F=12.61,P<0.001)。女性血清CA19-9和CA24-2水平与雌二醇水平呈负相关,方程式分别为Y=-0.027 01X+38.62(r2=0.523 9,P<0.001)和Y=-0.016 88X+23.40(r2=0.653 1,P<0.001)。分别随访CA19-9和CA24-2水平极高的10位女性5年,结果显示CA19-9和CA24-2水平多在6个月之后明显下降并至正常水平。结论:女性血清CA19-9和CA24-2水平存在非恶性升高,与女性生理周期有关。

CEA、CA199、CA724及HSP60在胃癌患者血清中的表达及应用价值

目的:探讨肿瘤标记物癌胚抗原(CEA)、糖类抗原19-9(CA199)、糖类抗原7-24(CA724)及热休克蛋白60(HSP60)在胃癌患者血清中的表达及应用价值。方法:采用电化学发光法和酶联免疫法检测胃癌组与正常对照组血清CEA、CA199、CA724及HSP60水平,分析其与胃癌淋巴转移、临床分期的关系及4项指标单一、联合检测的阳性率。结果:CEA、CA199、CA724及HSP60在胃癌组中的表达明显高于对照组,差异具有统计学意义(P<0.05);血清CEA、CA199、CA724及HSP60的阳性率与胃癌淋巴转移、临床分期有关(P<0.05);4项指标联合检测阳性率达74.36%,明显高于单项指标检测(P<0.05)。结论:胃癌患者血清CEA、CA199、CA724及HSP60水平与胃癌的发生、发展关系密切,联合检测血清水平变化对胃癌诊断及病情变化的判断具有重要意义。

单纯性肝囊肿伴囊液CA19-9水平超高表达1例报道

0引言CA19-9是诊断消化道肿瘤时常用的标志物之一,在慢性胰腺炎、胰腺癌、囊腺癌等多种常见的良、恶性病变患者的血清或体液中均可有异常表达,但仅在单纯性肝囊肿(simple liver cyst)囊液中呈超高水平表达则较为罕见。现将天津医科大学肿瘤医院收治的1例患者情况报道如下,以加强对该病的认识,减少误诊误治。

11th INTERNATIONAL CONFERENCE ON HYDRODYNAMICS 19-24 October 2014 SINGAPORE First Announcement and Call for Paper

CONFERENCE THEMES The overall aim of the ICHD Conference is to provide a forum for participants from around the world to review, discuss and present the latest developments in the broad discipline of hydrodynamics and fluid mechanics.

11th INTERNATIONAL CONFERENCE ON HYDRODYNAMICS 19-24 October 2014 SINGAPORE First Announcement and Call for Paper

CONFERENCE THEMES The overall aim of the ICHD Conference is to provide a forum for participants from around the world to review, discuss and present the latest deve- lopments in the broad discipline of hydrodynamics and fluid mechanics. The first International Conference on Hydrodynamics （ICHD） was initiated in 1994 in Wuxi, China. Since then, 9 more ICHD conferences were held subsequently in Hong Kong, Seoul, Yokohama, Tainan, Perth, Ischia, Nantes, Shanghai and St Petersburg.

11th INTERNATIONAL CONFERENCE ON HYDRODYNAMICS 19-24 October 2014 SINGAPORE First Announcement and Call for Paper

The overall aim of the ICHD Conference is to provide a forum for participants from around the world to review, discuss and present the latest deve- lopments in the broad discipline of hydrodynamics and fluid mechanics.

水稻施肥效果及肥料利用率研究

为了明确不同肥料对水稻产量及植株氮磷钾含量的影响,选用天禾水稻专用配方肥(24-7-19)和雅冉复合肥(15-15-15)作为试验材料,进行了施肥效果及肥料利用率研究。结果表明:2种肥料均可以提高水稻产量,各养分元素对产量的贡献率大致表现为氮>钾>磷,表明水稻生产中要特别注意氮素和钾素的投入;总体来看,天禾水稻专用配方肥(24-7-19)的表观利用率、生理效率、农学效率、产量贡献率和偏生产力均高于雅冉复合肥(15-15-15),表明水稻生产中可使用水稻专用配方肥替代普通复合肥。

含有大的吸电子取代基化合物GeR_2Cl_2(R=Ar,Ar′和Ar″)的合成、晶体结构与^(19)F NMR

GeCl4与ArLi和Ar′Li(Ar″Li与Ar′Li 1∶1的混合物)反应生成化合物GeAr2Cl2,GeArCl3和GeAr″2Cl2,测定了GeAr2Cl2和GeAr″2Cl2的晶体结构,并进行了19F NMR表征.

血清CA19-9、CA24-2、CA72-4水平与胃癌病程发展的关系

目的:测定胃癌患者血清中糖链抗原19-9(CA19-9)、糖链抗原24-2(CA24-2)、糖链抗原72-4(CA72-4)的水平,探讨CA19-9、CA24-2、CA72-4在胃癌不同病程分期中的临床意义。方法:采用电化学发光法分别测定65例正常对照组、69例良性胃病组、157例胃癌患者血清中CA19-9、CA24-2、CA72-4的含量,组间采用SPSS13.0统计软件对数据进行分析处理。结果:血清中CA19-9、CA24-2、CA72-4水平与诊断阳性可随胃癌的TNM分期递增而明显升高。不同病程分期的胃癌患者血清CA19-9、CA24-2、CA72-4差异有统计学意义(P<0.05)。血清CA19-9、CA24-2、CA72-4水平与胃癌分化程度具有统计学意义(P<0.05;r=0.7243;r=0.8701;r=0.9213)。结论:不同病程分期胃癌患者血清中CA19-9、CA24-2、CA72-4的水平具有显著性差异,与胃癌分化程度有关,癌组织分期越高、分化程度越差血清CA19-9、CA24-2、CA72-4的水平越高。血清CA19-9、CA24-2、CA72-4的水平变化对判断进展期胃癌病情演变、疗效及预后具有临床意义。

毛冬青化学成分研究

目的研究江西产毛冬青根的化学成分,阐明其药理作用的物质基础。方法通过反复的硅胶、反相硅胶以及SephadexLH-20柱色谱等方法分离纯化化合物,利用核磁共振等现代波谱技术解析化合物结构。结果从毛冬青根中分得14个化合物,并鉴定结构为3-O-β-D-吡喃木糖-3β,19α,24-三羟基取代齐墩果酸-28-β-D-吡喃葡萄糖酯苷(3-O-β-D-xylopyranosylspathod-icacid28-β-D-glucopyranosylester,1),丁香脂素-4-O-β-D-葡萄糖苷(syringaresinolmono-β-D-glucoside,2),广玉兰赖宁苷C(magnoleninC,3),丁香脂素-4,4′-O-β-D-双葡萄苷(liriodendrin,4)、ilexsaponinA1(5)、ilexsaponinB1(6)、ilexsaponinB2(7)、il-exosideA(8)、ilexosideO(9)、ilexgeninA(10)、ilexgeninB3-O-β-D-xylopyranoside(11)、肌醇(myoinositol,12)、β-谷甾醇(β-sitosterol,13)和胡萝卜苷(daucosterol,14)。结论化合物1为首次发现的新化合物,2为首次从冬青属植物中分离得到,3为首次从该植物中分离得到。

尾叶香茶菜三萜类化学成分研究

为了研究尾叶香茶菜乙酸乙酯提取物的化学成分,本文采用十八烷基键合硅胶(octadecyl binded silica gel,ODS)柱色谱和制备高效液相色谱(high performance liquid chromatography,HPLC)方法对尾叶香茶菜乙酸乙酯提取物进行分离,根据理化性质和波谱数据鉴定化合物的结构。研究结果表明,从尾叶香茶菜乙酸乙酯提取物中分离得到7个三萜类化合物,分别鉴定为2α,3β,19α-三羟基-28-降乌苏-12-烯;2α,3β,24-三羟基齐墩果-12-烯-28-酸;山香三萜二烯酸;2α,3α-二羟基乌苏-12-烯-28-酸;3-表马斯里酸;马斯里酸;齐墩果酸。其中,化合物2α,3β,19α-三羟基-28-降乌苏-12-烯和2α,3β,24-三羟基齐墩果-12-烯-28-酸为首次从香茶菜属植物中分离得到。该研究具有一定的实际意义。